##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146574_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1144561
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.242989233426616	31.0	31.0	34.0	28.0	34.0
2	31.482322916821385	33.0	31.0	34.0	28.0	34.0
3	31.66185900096194	33.0	31.0	34.0	29.0	34.0
4	35.17866500780649	37.0	35.0	37.0	32.0	37.0
5	35.23109995884885	37.0	35.0	37.0	32.0	37.0
6	35.271769700347996	37.0	35.0	37.0	32.0	37.0
7	35.18551566932649	37.0	35.0	37.0	32.0	37.0
8	35.16992628614814	37.0	35.0	37.0	32.0	37.0
9	36.82206540324194	39.0	37.0	39.0	33.0	39.0
10-11	36.7908953738595	39.0	37.0	39.0	33.0	39.0
12-13	36.75185988339634	39.0	37.0	39.0	33.0	39.0
14-15	38.14554532261714	40.0	38.0	41.0	33.0	41.0
16-17	38.16274798809325	40.0	38.0	41.0	33.0	41.0
18-19	38.20238458238573	40.0	38.5	41.0	33.0	41.0
20-21	38.27684894033608	40.0	39.0	41.0	33.0	41.0
22-23	38.29503757335782	40.0	39.0	41.0	33.0	41.0
24-25	38.33785661052578	40.0	38.5	41.0	33.0	41.0
26-27	38.34597893864984	40.0	38.0	41.0	33.0	41.0
28-29	38.29610654215896	40.0	38.0	41.0	33.0	41.0
30-31	38.28691349783891	40.0	38.0	41.0	33.0	41.0
32-33	38.29395025691073	40.0	38.0	41.0	33.0	41.0
34-35	38.22981780787568	40.0	38.0	41.0	33.0	41.0
36-37	38.134878350738845	40.0	38.0	41.0	33.0	41.0
38-39	38.08419079454917	40.0	38.0	41.0	33.0	41.0
40-41	38.02451158129624	40.0	38.0	41.0	32.0	41.0
42-43	37.9133777055133	40.0	38.0	41.0	32.0	41.0
44-45	37.80166981052124	40.0	38.0	41.0	32.0	41.0
46-47	37.69524603756375	40.0	37.0	41.0	32.0	41.0
48-49	37.572939319092654	40.0	37.0	41.0	31.0	41.0
50-51	37.44854577431871	40.0	37.0	41.0	31.0	41.0
52-53	37.31181256394373	40.0	36.0	41.0	31.0	41.0
54-55	37.14353013950327	40.0	36.0	41.0	31.0	41.0
56-57	37.011813699750384	39.0	35.0	41.0	31.0	41.0
58-59	36.78770113606876	39.0	35.0	41.0	30.0	41.0
60-61	36.60245806033929	39.0	35.0	41.0	30.0	41.0
62-63	36.35528600048403	38.5	35.0	40.5	30.0	41.0
64-65	36.09545974395424	38.0	35.0	40.0	30.0	41.0
66-67	35.837659591756136	37.0	35.0	40.0	30.0	41.0
68-69	35.5551185126874	37.0	35.0	40.0	29.0	41.0
70-71	35.278386210957734	36.0	34.5	39.0	29.0	41.0
72-73	34.95545016823044	36.0	34.0	39.0	29.0	41.0
74-75	34.631348613136396	35.0	34.0	38.5	29.0	40.0
76-77	34.30140857499076	35.0	34.0	37.0	29.0	39.5
78-79	34.027973170499436	35.0	34.0	37.0	28.5	39.0
80-81	33.754015294947145	35.0	34.0	36.5	28.0	39.0
82-83	33.50163031939757	35.0	34.0	36.0	28.0	37.5
84-85	33.28830311359552	35.0	34.0	36.0	28.0	37.0
86-87	33.089715183375986	35.0	33.0	35.0	27.0	37.0
88-89	32.929932087499054	35.0	33.0	35.0	27.0	36.0
90-91	32.768897856907586	35.0	33.0	35.0	27.0	36.0
92-93	32.68868719098414	35.0	33.0	35.0	27.0	36.0
94-95	32.62621695130272	35.0	33.0	35.0	27.0	36.0
96-97	32.56053631042819	35.0	33.0	35.0	27.0	35.0
98-99	32.50171899968635	35.0	33.0	35.0	27.0	35.0
100	31.549117085065802	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22306408350076268
1101	2	0.28733932826033026
1101	3	0.3693720676198602
1101	4	0.36460643822513816
1101	5	0.26861691972789004
1101	6	0.31645198792399754
1101	7	0.37098660686996254
1101	8	0.37472461183536865
1101	9	0.5456930375563402
1101	10-11	0.6916288066099483
1101	12-13	0.6208118740068116
1101	14-15	0.7015963884039635
1101	16-17	0.747846331265805
1101	18-19	0.6649443400459276
1101	20-21	0.6823713575671491
1101	22-23	0.6258799351430326
1101	24-25	0.6861917623204619
1101	26-27	0.6594007507369568
1101	28-29	0.7722971323359431
1101	30-31	0.6796188378211241
1101	32-33	0.7634017077519175
1101	34-35	0.7909949740955113
1101	36-37	0.8131635374480837
1101	38-39	0.8246726601459855
1101	40-41	0.7263750145868926
1101	42-43	0.6904409135683025
1101	44-45	0.5990387963971813
1101	46-47	0.5785141766337176
1101	48-49	0.6353283889871619
1101	50-51	0.6526898211985994
1101	52-53	0.6951393801737993
1101	54-55	0.7043911759119652
1101	56-57	0.7066299649476662
1101	58-59	0.6313101311426053
1101	60-61	0.6870025223750815
1101	62-63	0.6428503128472158
1101	64-65	0.5828711085717728
1101	66-67	0.6432326959475603
1101	68-69	0.6883307466247501
1101	70-71	0.6800962725179929
1101	72-73	0.6477892199525073
1101	74-75	0.5721683469284073
1101	76-77	0.5597498584560725
1101	78-79	0.5166965914716499
1101	80-81	0.5607320692300988
1101	82-83	0.5451647124372911
1101	84-85	0.5672538118631394
1101	86-87	0.5699545297903086
1101	88-89	0.49908835675002905
1101	90-91	0.5345633449525451
1101	92-93	0.3877979799631106
1101	94-95	0.5266049699125475
1101	96-97	0.5946647471872737
1101	98-99	0.46447055682397576
1101	100	0.4855827848806449
1102	1	0.1762905029454842
1102	2	0.09604808553246613
1102	3	0.11949833762431439
1102	4	0.0697407238268255
1102	5	0.12308456765404685
1102	6	0.1277178543446027
1102	7	0.11412102927359058
1102	8	0.27066499155588275
1102	9	0.12492469080780921
1102	10-11	0.17593146324024644
1102	12-13	0.1259795447181986
1102	14-15	0.2812407124143732
1102	16-17	0.23328779611563277
1102	18-19	0.2552279594629283
1102	20-21	0.1687387269518723
1102	22-23	0.13946228424981655
1102	24-25	0.20291059038986958
1102	26-27	0.23859933596931882
1102	28-29	0.23524034005156835
1102	30-31	0.161632779841554
1102	32-33	0.23305364646645899
1102	34-35	0.309930718404523
1102	36-37	0.2647354234712296
1102	38-39	0.2537442505954033
1102	40-41	0.33059476561641077
1102	42-43	0.29566131191562306
1102	44-45	0.17614526318805446
1102	46-47	0.18789253864336786
1102	48-49	0.2837954145097896
1102	50-51	0.3350178188032018
1102	52-53	0.1937464464923977
1102	54-55	0.11289264653284192
1102	56-57	0.18606029810568714
1102	58-59	0.08856292165308588
1102	60-61	0.09539595570066695
1102	62-63	0.11624935641138023
1102	64-65	0.12301976064230047
1102	66-67	0.23013907404366307
1102	68-69	0.08968590122680098
1102	70-71	0.22033099203550677
1102	72-73	0.2734440686527293
1102	74-75	0.2567042010413658
1102	76-77	0.16291369780710596
1102	78-79	0.2683188496006039
1102	80-81	0.2195097484078019
1102	82-83	0.15176173694974437
1102	84-85	0.20748305024324054
1102	86-87	0.14052145606358124
1102	88-89	0.11424524185134288
1102	90-91	0.17047833930607226
1102	92-93	0.24535431676949315
1102	94-95	0.1940206498612298
1102	96-97	0.016137488041366055
1102	98-99	0.09249746966165873
1102	100	-0.06897922014660196
1103	1	0.08769682803395895
1103	2	-0.03619433770655078
1103	3	0.06579821706884559
1103	4	-0.07588653713285254
1103	5	-7.72998258248947E-4
1103	6	0.033261968192192626
1103	7	0.11701444769430935
1103	8	0.12933189291432257
1103	9	0.18628163041548618
1103	10-11	0.08772250274906668
1103	12-13	0.10045485393840892
1103	14-15	0.11181191529829704
1103	16-17	0.06471425880377524
1103	18-19	0.07741670131606782
1103	20-21	0.019636183349085456
1103	22-23	-0.003305977812601668
1103	24-25	0.09687253459625822
1103	26-27	0.07900319461574412
1103	28-29	0.08673316706645551
1103	30-31	0.08584316765373501
1103	32-33	0.040088430662585495
1103	34-35	0.04581381227528425
1103	36-37	0.05633479416034248
1103	38-39	0.13685140989984745
1103	40-41	0.043736575360100005
1103	42-43	0.1211756768383836
1103	44-45	-0.04179785223193022
1103	46-47	0.028042825610697264
1103	48-49	-0.025377954887076726
1103	50-51	-0.012249467095379885
1103	52-53	-0.0437951020114653
1103	54-55	-0.01960814097714092
1103	56-57	-0.14788859282319322
1103	58-59	0.002524351745073261
1103	60-61	-0.20462639520506798
1103	62-63	-0.10531630492925359
1103	64-65	-0.13469903533662375
1103	66-67	0.006106970649156551
1103	68-69	-0.09292056293397621
1103	70-71	-0.187407475354604
1103	72-73	-0.18978629511806133
1103	74-75	-0.2294201562611704
1103	76-77	-0.32268499930501093
1103	78-79	-0.14782386332880293
1103	80-81	-0.16506176555890306
1103	82-83	-0.23866741351105958
1103	84-85	-0.13134513490242483
1103	86-87	-0.06497181274113473
1103	88-89	-0.1251233733815198
1103	90-91	-0.2302777574154078
1103	92-93	-0.22322871858806792
1103	94-95	-0.25350068697563444
1103	96-97	-0.236881953761106
1103	98-99	-0.2597292774450324
1103	100	-0.32179976938634525
1104	1	0.02190389058188913
1104	2	0.042216620802683735
1104	3	0.14493617638816758
1104	4	0.12538077576618178
1104	5	0.0822068318143181
1104	6	0.07861155808318898
1104	7	0.08084626336929546
1104	8	0.0721162932997359
1104	9	0.08246252347769456
1104	10-11	0.09419005456217633
1104	12-13	0.0572879621063791
1104	14-15	0.24822248974763284
1104	16-17	0.21737870617506871
1104	18-19	0.17184075347020666
1104	20-21	0.1919644129812994
1104	22-23	0.19658321285110247
1104	24-25	0.2682666847619828
1104	26-27	0.32497130747000824
1104	28-29	0.2593500774868218
1104	30-31	0.3234787490657425
1104	32-33	0.325954899053734
1104	34-35	0.20874507954154353
1104	36-37	0.2105223400218037
1104	38-39	0.17514400504687444
1104	40-41	0.31610838265019936
1104	42-43	0.2156681900017574
1104	44-45	0.15374226274017389
1104	46-47	0.15416008596643138
1104	48-49	0.20301284683585408
1104	50-51	0.16333733701330289
1104	52-53	0.06418465387734784
1104	54-55	0.20503002245348512
1104	56-57	0.11375899872697914
1104	58-59	0.1766111603047804
1104	60-61	0.14684267631528058
1104	62-63	0.13987732951099474
1104	64-65	0.17654972314522865
1104	66-67	0.18652991979682554
1104	68-69	0.021275791718629478
1104	70-71	0.14636940618919425
1104	72-73	0.16058422095077418
1104	74-75	0.1685070224203642
1104	76-77	0.19533275138415718
1104	78-79	0.1165100280076743
1104	80-81	0.06924466083152936
1104	82-83	0.15388674169068395
1104	84-85	0.1782202881760142
1104	86-87	0.2552945111824414
1104	88-89	0.05522729959232464
1104	90-91	0.1059711388362814
1104	92-93	0.1794608452835078
1104	94-95	0.12118048183819496
1104	96-97	0.14177058944881082
1104	98-99	0.18269692137506865
1104	100	0.09703451130888041
1105	1	0.06996548161396632
1105	2	0.10933592813641013
1105	3	0.1629011641120357
1105	4	0.17531068377143555
1105	5	0.16454445202650447
1105	6	0.1449014836234923
1105	7	0.1438704479175854
1105	8	0.13298553038089267
1105	9	0.3171558930800913
1105	10-11	0.2904097034438422
1105	12-13	0.3432257656978521
1105	14-15	0.27506880762967967
1105	16-17	0.19904700002511788
1105	18-19	0.17598364800464594
1105	20-21	0.1756925122437707
1105	22-23	0.2956776772695804
1105	24-25	0.1968960932112367
1105	26-27	0.15675955256878638
1105	28-29	0.33108176725787786
1105	30-31	0.2688981207909791
1105	32-33	0.31619601390985963
1105	34-35	0.24131296761274257
1105	36-37	0.24360608269345363
1105	38-39	0.2655428068189991
1105	40-41	0.3535766887041234
1105	42-43	0.3378946184573408
1105	44-45	0.27019372905185435
1105	46-47	0.26551181771240806
1105	48-49	0.2336477855675767
1105	50-51	0.28105836766268766
1105	52-53	0.37561350287526807
1105	54-55	0.4025599821646466
1105	56-57	0.28172286240969413
1105	58-59	0.24933983443400365
1105	60-61	0.09062215228729542
1105	62-63	0.18409409763575724
1105	64-65	0.14891898511586277
1105	66-67	0.2149549133335853
1105	68-69	0.241784765581464
1105	70-71	0.0883959801278138
1105	72-73	0.06648772522935786
1105	74-75	0.06949023727441528
1105	76-77	0.08127629561437288
1105	78-79	0.04390078325092617
1105	80-81	0.08739752400521184
1105	82-83	0.14262405006173395
1105	84-85	0.09240034832827604
1105	86-87	0.13589103179334927
1105	88-89	0.05284513523502454
1105	90-91	0.09655287830602788
1105	92-93	0.11105571515968649
1105	94-95	0.07346292768750828
1105	96-97	0.08177047704469942
1105	98-99	-0.05214217213391237
1105	100	0.04641974108697866
1106	1	0.2435130666572931
1106	2	0.12093933800935375
1106	3	0.2934401726737157
1106	4	0.19257760548791936
1106	5	0.29086662878046354
1106	6	0.21931596670362552
1106	7	0.15171359968208975
1106	8	0.28303991963031905
1106	9	0.33345638813325706
1106	10-11	0.32206221163922777
1106	12-13	0.32973852682174964
1106	14-15	0.3800502338859957
1106	16-17	0.3751277551106966
1106	18-19	0.3085972179373755
1106	20-21	0.35169889835563595
1106	22-23	0.36435039458608287
1106	24-25	0.4308412747677295
1106	26-27	0.3649184949753774
1106	28-29	0.35798983092660563
1106	30-31	0.3294629549962593
1106	32-33	0.282692791254334
1106	34-35	0.23213524426532217
1106	36-37	0.315511630396891
1106	38-39	0.22581553005542077
1106	40-41	0.34399678090684915
1106	42-43	0.348806692870717
1106	44-45	0.25228227622990573
1106	46-47	0.2317917780489509
1106	48-49	0.14838172223925739
1106	50-51	0.15320570702940728
1106	52-53	0.3898401765471675
1106	54-55	0.3856854400333276
1106	56-57	0.24632027096545528
1106	58-59	0.21708589953830426
1106	60-61	0.2644087540321891
1106	62-63	0.26416228769473094
1106	64-65	0.18180135633914318
1106	66-67	0.18117548948963957
1106	68-69	0.2220312931793842
1106	70-71	0.2522506239337332
1106	72-73	0.2626455132518757
1106	74-75	0.29234334190156375
1106	76-77	0.3138299263742681
1106	78-79	0.30066188494772206
1106	80-81	0.2408332965915463
1106	82-83	0.25919741634928783
1106	84-85	0.23186566870685255
1106	86-87	0.19919327322241998
1106	88-89	0.06138461739017487
1106	90-91	0.07283435562163731
1106	92-93	0.04585077498786205
1106	94-95	0.1283993995642021
1106	96-97	0.16765406185123055
1106	98-99	0.15187116248206678
1106	100	0.21366261313534451
1107	1	0.025642579001296184
1107	2	0.06618503604440562
1107	3	0.06751018258843544
1107	4	0.12016169536255461
1107	5	0.04989202245833013
1107	6	-0.0032885347906415063
1107	7	-0.014701611826041017
1107	8	0.12514395714394766
1107	9	0.0032246095172965283
1107	10-11	0.15466293777774354
1107	12-13	0.09926187933765362
1107	14-15	0.02763929876213922
1107	16-17	0.05453424801690687
1107	18-19	0.021852774237814288
1107	20-21	0.01967982398819146
1107	22-23	0.05675469637461106
1107	24-25	0.02441928878947408
1107	26-27	-0.04160563731026201
1107	28-29	0.08396286712414991
1107	30-31	-0.021577384856328763
1107	32-33	0.09980291520596296
1107	34-35	0.07014160944454773
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2216	44-45	-0.47099684431726274
2216	46-47	-0.3991739612583345
2216	48-49	-0.47680020590612315
2216	50-51	-0.48591447556928813
2216	52-53	-0.42154919590652185
2216	54-55	-0.5585838071116171
2216	56-57	-0.4724579427078339
2216	58-59	-0.5116952697756361
2216	60-61	-0.5603562595155793
2216	62-63	-0.45158346471111344
2216	64-65	-0.5273030585026035
2216	66-67	-0.6006013583391407
2216	68-69	-0.578767380818654
2216	70-71	-0.594085097782937
2216	72-73	-0.530243762199504
2216	74-75	-0.5070510772759818
2216	76-77	-0.48756651618018054
2216	78-79	-0.6016219008243198
2216	80-81	-0.38086605355812253
2216	82-83	-0.44474569472941994
2216	84-85	-0.4389764805516947
2216	86-87	-0.5180351170731896
2216	88-89	-0.5585011151985668
2216	90-91	-0.6074317301596466
2216	92-93	-0.5278587363158351
2216	94-95	-0.5248698384664294
2216	96-97	-0.4951863739169724
2216	98-99	-0.6061041846104018
2216	100	-0.6627939513165018
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	34.0
1	20.0
2	21.0
3	13.0
4	15.0
5	19.0
6	41.0
7	211.0
8	686.0
9	934.0
10	1037.0
11	1187.0
12	1561.0
13	1901.0
14	2155.0
15	2389.0
16	2576.0
17	2952.0
18	3181.0
19	3514.0
20	4002.0
21	4462.0
22	5120.0
23	6065.0
24	7379.0
25	9394.0
26	10499.0
27	10201.0
28	10726.0
29	13195.0
30	15268.0
31	17242.0
32	21875.0
33	29249.0
34	44070.0
35	70325.0
36	128319.0
37	268933.0
38	333073.0
39	110574.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58294225026053	21.094706215598094	13.567989005378411	23.754362528762965
2	14.161911078140196	21.224123875107804	39.942574684803	24.671390361949
3	18.294611711906942	27.169593361808026	30.366704881475837	24.169090044809195
4	12.153081785406586	17.52237350515626	39.921742975130066	30.40280173430709
5	12.82663176070073	39.02428154993673	35.483673654850364	12.665413034512166
6	29.264366892053665	38.592295364740714	18.70251887011732	13.440818873088304
7	25.563794810230654	33.49174021271781	23.310537770822904	17.63392720622863
8	24.683154431906054	35.00674976080945	21.560614599579726	18.749481207704775
9	25.698298150832578	16.283910067708728	21.127937081298533	36.889854700160164
10-11	23.968470885111483	26.364872350947955	29.198464611443242	20.468192152497323
12-13	25.279909268679695	24.386008293737408	28.17542145851646	22.15866097906644
14-15	22.73541294119652	25.7964114028768	26.236368219826367	25.23180743610031
16-17	21.759525846288007	28.475392747014038	27.27034817932629	22.494733227371665
18-19	21.59872987967672	28.973920854273004	28.073101820642687	21.354247445407587
20-21	22.894490232356972	28.174107172112794	26.78048961634996	22.150912979180273
22-23	22.64823873195166	27.74565640671	26.40729102457296	23.198813836765382
24-25	22.640580473712195	27.97380721153573	26.879586828814162	22.50602548593791
26-27	22.868494079490013	27.565408703918582	26.893016664103676	22.673080552487733
28-29	22.170747935846652	27.97816294174673	27.36976250713754	22.481326615269076
30-31	22.569893323418736	28.09840266925192	26.915029658597366	22.41667434873198
32-33	22.406566851433638	28.327979067025105	26.532475776914744	22.732978304626513
34-35	22.724814116730062	27.96393047541902	26.947372468216997	22.36388293963392
36-37	23.000862512682083	27.57829683893908	27.027669441796125	22.39317120658271
38-39	23.10829546144714	27.463070814637707	27.23455052068109	22.194083203234065
40-41	22.580594420031712	27.516995819808265	27.515204371483463	22.387205388676563
42-43	22.377308247093374	27.865494942273887	27.00116540287239	22.756031407760354
44-45	22.558928444234887	28.15547556798145	26.718452236071233	22.567143751712432
46-47	22.380807548469253	27.772708640868256	26.645481170341355	23.20100264032114
48-49	22.862355653152417	27.506276424895937	26.978117181781435	22.65325074017021
50-51	22.587990985569835	27.794755216793376	26.953312261206968	22.663941536429828
52-53	22.315287253251668	27.87974290803386	26.9080006502462	22.89696918846827
54-55	22.183735347819663	27.64372924702368	27.070577818799368	23.10195758635729
56-57	22.943433860493816	27.671533290125605	26.51816669296132	22.866866156419256
58-59	22.59298040751255	27.51478876193939	27.18156089613604	22.710669934412014
60-61	22.603591618973034	27.45767882053093	27.20355071374381	22.73517884675223
62-63	22.934012148756718	27.301490189223443	26.78232749202465	22.982170169995193
64-65	22.28147626322837	27.682033191974355	27.213905388539082	22.822585156258192
66-67	22.77115957869417	27.296600543916473	26.927798431880866	23.004441445508494
68-69	22.234226013322463	27.830236093010736	26.904866167926627	23.030671725740177
70-71	22.34025955296056	27.455843437863965	26.6897273579484	23.514169651227075
72-73	22.80138620611294	27.22924842456714	26.899696712786138	23.069668656533786
74-75	22.71713773521743	27.796759202203305	26.80082440274067	22.6852786598386
76-77	22.745502673944024	27.18302171189231	26.948819439618948	23.122656174544716
78-79	22.506331271345505	27.447978691092956	27.16177682022686	22.88391321733468
80-81	22.395138398739583	27.74171978489031	27.14599444525782	22.717147371112283
82-83	22.71432492151063	27.269286708201353	27.326230832162114	22.690157538125906
84-85	22.84844384894466	27.42997560606815	26.584797100347245	23.13678344463993
86-87	22.487696203062686	27.593892107652866	27.159817822522918	22.758593866761526
88-89	23.165716428222147	26.839505622553666	27.01792933232476	22.97684861689943
90-91	22.752547279144036	26.961173330350075	27.31606188634127	22.97021750416462
92-93	22.75629035810553	27.531919393916088	27.014146969056018	22.69764327892236
94-95	22.17714092924956	27.55203626237429	27.12069511041809	23.150127697958055
96-97	22.268923721273335	27.336939516460312	26.974442072952225	23.419694689314127
98-99	22.428274148453525	27.522039743642406	26.766828383221913	23.282857724682156
100	22.43386715049309	27.486951718737433	26.866961716884635	23.212219413884842
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1289.0
1	881.0
2	458.5
3	403.5
4	340.0
5	282.0
6	217.5
7	191.0
8	191.0
9	203.0
10	181.0
11	148.0
12	151.5
13	152.5
14	171.5
15	201.5
16	209.5
17	258.5
18	388.5
19	505.5
20	607.5
21	836.0
22	1160.5
23	1679.0
24	2321.5
25	2970.0
26	4062.0
27	5505.0
28	7040.0
29	9405.5
30	12133.5
31	14465.5
32	17507.0
33	21225.5
34	24570.5
35	27345.5
36	30430.5
37	34064.0
38	35189.0
39	34741.5
40	34895.0
41	35037.5
42	35406.0
43	36431.5
44	38213.0
45	40870.0
46	43280.5
47	47220.0
48	60300.0
49	60229.5
50	49735.5
51	47377.0
52	43571.5
53	40573.5
54	37849.0
55	33535.5
56	29472.5
57	25563.0
58	21759.5
59	19858.0
60	17163.0
61	13229.0
62	10231.5
63	7916.5
64	5720.0
65	3992.0
66	2961.0
67	2135.0
68	1586.5
69	1237.0
70	939.5
71	700.5
72	570.0
73	430.0
74	306.5
75	243.0
76	187.5
77	130.0
78	76.0
79	40.0
80	25.5
81	21.5
82	13.0
83	10.5
84	12.0
85	8.0
86	7.5
87	7.5
88	6.0
89	4.0
90	3.0
91	5.0
92	4.5
93	4.0
94	2.0
95	0.0
96	0.5
97	3.0
98	4.5
99	3.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0636925423808779
2	0.010309629630923996
3	0.013367570623147217
4	0.011095957314638537
5	0.013367570623147217
6	0.01389178907895691
7	0.003494789705397965
8	0.006640100440256133
9	0.008736974263494913
10-11	0.008300125550320166
12-13	0.007251688638700777
14-15	0.01140175141386086
16-17	0.010659108601463792
18-19	0.008955398620082284
20-21	0.008169070936367743
22-23	0.002664777150365948
24-25	0.004150062775160083
26-27	0.005679033271271693
28-29	0.0073827432526532
30-31	0.010528053987511369
32-33	0.012799667296020046
34-35	0.01397915882159186
36-37	0.0200950408060383
38-39	0.021187162588975163
40-41	0.020575574390530517
42-43	0.02808937225713614
44-45	0.031059943506724412
46-47	0.033375241686550566
48-49	0.034816842440027224
50-51	0.03455473321212238
52-53	0.033113132458645715
54-55	0.0391416447004572
56-57	0.04154431262291831
58-59	0.03922901444309215
60-61	0.03957849341363195
62-63	0.035908964222964086
64-65	0.03748161959039317
66-67	0.03944743879967953
68-69	0.04176273697950568
70-71	0.04180642185082315
72-73	0.037656359075663066
74-75	0.0401900816120766
76-77	0.04058324545393387
78-79	0.03831163214542519
80-81	0.04311696799034739
82-83	0.03992797238417175
84-85	0.03704477087721843
86-87	0.03512263653924955
88-89	0.03271996861678845
90-91	0.0348605273113447
92-93	0.03748161959039317
94-95	0.03975323289890185
96-97	0.03625844319350388
98-99	0.033025762716010765
100	0.030492040179597245
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1144561.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.62651559745346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.45764724342843	53.1564843669319
2	13.607486830152554	18.676688143832138
3	4.506600753147983	9.278169206322168
4	1.933280253472584	5.306971498767403
5	0.9548728637974251	3.276479874048953
6	0.5342611130592202	2.199868716508285
7	0.30888573794422375	1.4838426339002138
8	0.20071181347424163	1.101932191838657
9	0.13365721563932048	0.8255186094405625
>10	0.3591245901907873	3.399179265251135
>50	0.001420550085509617	0.065420992760262
>100	0.0014199477396431824	0.2498950184941348
>500	0.0	0.0
>1k	6.310878680404371E-4	0.9795494819042003
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	3391	0.29627079727511246	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	2652	0.23170455746788507	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1616	0.1411895040980778	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1388	0.12126920277730938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.736974263494912E-5
2	0.0	0.0	0.0	0.0	8.736974263494912E-5
3	0.0	0.0	0.0	0.0	8.736974263494912E-5
4	0.0	0.0	0.0	0.0	8.736974263494912E-5
5	0.0	0.0	0.0	0.0	8.736974263494912E-5
6	0.0	0.0	0.0	0.0	1.7473948526989825E-4
7	0.0	0.0	0.0	0.0	1.7473948526989825E-4
8	0.0	0.0	0.0	0.0	1.7473948526989825E-4
9	0.0	0.0	0.0	0.0	1.7473948526989825E-4
10-11	0.0	0.0	0.0	1.7473948526989825E-4	1.7473948526989825E-4
12-13	0.0	0.0	0.0	1.7473948526989825E-4	5.242184558096947E-4
14-15	0.0	0.0	0.0	1.7473948526989825E-4	5.242184558096947E-4
16-17	0.0	0.0	0.0	2.184243565873728E-4	6.115881984446438E-4
18-19	0.0	0.0	0.0	3.0579409922232195E-4	6.98957941079593E-4
20-21	0.0	0.0	0.0	3.9316384185727104E-4	7.426428123970675E-4
22-23	0.0	0.0	0.0	6.115881984446439E-4	8.736974263494912E-4
24-25	0.0	0.0	0.0	0.001179491525571813	8.736974263494912E-4
26-27	0.0	0.0	0.0	0.0020095040806038297	0.0010484369116193894
28-29	0.0	0.0	0.0	0.0049800753301921	0.0011358066542543385
30-31	0.0	0.0	0.0	0.01196965474098803	0.0011358066542543385
32-33	0.0	0.0	0.0	0.02511880100754787	0.0012231763968892876
34-35	0.0	0.0	0.0	0.048883371004254036	0.0016600251100640333
36-37	0.0	0.0	0.0	0.08640867546596467	0.0016600251100640333
38-39	0.0	0.0	0.0	0.13520467672758377	0.0016600251100640333
40-41	0.0	0.0	0.0	0.19138342124185603	0.0016600251100640333
42-43	0.0	0.0	0.0	0.24406737605073037	0.0016600251100640333
44-45	0.0	0.0	0.0	0.30732306971843354	0.0017037099813815078
46-47	0.0	0.0	0.0	0.3814999812155053	0.001791079724016457
48-49	0.0	0.0	0.0	0.45908431267534017	0.0018347645953339315
50-51	0.0	0.0	0.0	0.5360570559367304	0.0019221343379688806
52-53	0.0	0.0	0.0	0.6097534338493099	0.0021405586945562535
54-55	0.0	0.0	0.0	0.6925362649959242	0.002184243565873728
56-57	0.0	0.0	0.0	0.7861529442292722	0.002184243565873728
58-59	0.0	0.0	0.0	0.8944040553539742	0.002184243565873728
60-61	0.0	0.0	0.0	1.0084652543639003	0.002184243565873728
62-63	0.0	0.0	0.0	1.114313697566141	0.002184243565873728
64-65	0.0	0.0	0.0	1.2207737289668268	0.002184243565873728
66-67	0.0	0.0	0.0	1.3397276335643098	0.002184243565873728
68-69	0.0	0.0	0.0	1.4742333523508138	0.002184243565873728
70-71	0.0	0.0	0.0	1.6220629568891478	0.002184243565873728
72-73	0.0	0.0	0.0	1.7705041496259264	0.002358983051143626
74-75	0.0	0.0	0.0	1.9186832331348	0.0025337225364135247
76-77	0.0	0.0	0.0	2.0799240931675986	0.0026210922790484738
78-79	0.0	0.0	0.0	2.256673082518101	0.0026210922790484738
80-81	0.0	0.0	0.0	2.4483622978591795	0.0026210922790484738
82-83	0.0	0.0	0.0	2.6445510549459574	0.002795831764318372
84-85	0.0	0.0	0.0	2.8448898748078957	0.002795831764318372
86-87	0.0	0.0	0.0	3.058028361965854	0.002883201506953321
88	0.0	0.0	0.0	3.217740251502541	0.00297057124958827
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATCGT	525	0.0	27.750265	8
GTACTAG	535	0.0	27.248234	1
GTGATCG	480	0.0	25.456392	8
CATCGTC	655	0.0	24.395086	9
TTAAGGT	355	0.0	23.82917	4
GTCTAGG	185	2.8002432E-7	22.877115	1
TGATCGC	545	0.0	22.42031	9
TAGGCAT	795	0.0	21.281397	5
CTAGGCA	975	0.0	20.726625	4
ATAAGGT	650	0.0	20.24461	3
GGCATCG	710	0.0	19.857641	7
CAATGCG	335	0.0	19.640293	18-19
TCTAACG	360	5.456968E-12	19.58184	2
CATGGGG	2620	0.0	19.551945	4
TGCGCGA	385	0.0	19.532686	94
GTATAAG	915	0.0	19.529583	1
CGACGAG	350	0.0	19.469093	24-25
GTATTAT	365	7.2759576E-12	19.325418	1
GTACATG	5695	0.0	18.991734	1
TACATGG	5585	0.0	18.9332	2
>>END_MODULE