FastQCFastQC Report
Mon 16 Jan 2017
ERR1146574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144561
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39150.34205254241582583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.30885204021454515No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33510.2927760075697145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.1727299811892944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4600.030.681321
GTTACGT650.006145303628.93163
CTAACGC2600.027.1233753
TTCGACG350.00883830126.85684238-39
TAACGCC2950.025.499044
TCTAACG3000.025.0872122
GAGTACT40550.023.23793612-13
GGGCTAT8400.022.3807036
AGTACTT43250.021.51558112-13
GTACTTT46450.020.23570314-15
CATGGGG26050.020.2132514
GGACGCA1200.005064446519.5831156
GCTACGC600.00506724419.58225832-33
TAGGCAT8750.019.3428425
TAGTACT6600.019.2329394
TACCTGG7900.019.0535772
CGCCTAC3801.2732926E-1118.5516157
GCTATTG9200.018.3902978
ACTTTTT51850.018.21886416-17
AGGGCTA10350.018.1696055
Produced by FastQC (version 0.11.5)