##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146574_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1144561 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.535110841624 34.0 31.0 34.0 31.0 34.0 2 32.61080886034034 34.0 31.0 34.0 31.0 34.0 3 32.72967364780033 34.0 31.0 34.0 31.0 34.0 4 36.165448586838096 37.0 37.0 37.0 35.0 37.0 5 36.19323129129859 37.0 37.0 37.0 35.0 37.0 6 36.278332041717306 37.0 37.0 37.0 35.0 37.0 7 36.2636661567186 37.0 37.0 37.0 35.0 37.0 8 36.27970287297925 37.0 37.0 37.0 35.0 37.0 9 38.09896720227231 39.0 39.0 39.0 37.0 39.0 10-11 38.06734110283331 39.0 38.0 39.0 36.0 39.0 12-13 38.040553976590154 39.0 38.0 39.0 35.0 39.0 14-15 39.47180097871586 41.0 39.0 41.0 36.5 41.0 16-17 39.471998871182926 41.0 39.0 41.0 36.5 41.0 18-19 39.53522748023042 41.0 39.5 41.0 37.0 41.0 20-21 39.561370254621636 41.0 40.0 41.0 37.0 41.0 22-23 39.5199312225386 41.0 39.0 41.0 37.0 41.0 24-25 39.50900476252467 41.0 39.0 41.0 36.0 41.0 26-27 39.42127855133977 41.0 39.0 41.0 36.0 41.0 28-29 39.33254409332486 41.0 39.0 41.0 36.0 41.0 30-31 39.25714575282575 41.0 39.0 41.0 36.0 41.0 32-33 39.14229735243469 41.0 39.0 41.0 35.0 41.0 34-35 39.07830687923143 41.0 39.0 41.0 35.0 41.0 36-37 38.99747501443785 41.0 39.0 41.0 35.0 41.0 38-39 38.88779802911334 40.0 38.5 41.0 35.0 41.0 40-41 38.80462902370428 40.0 38.0 41.0 35.0 41.0 42-43 38.738342473664574 40.0 38.0 41.0 35.0 41.0 44-45 38.59981468877587 40.0 38.0 41.0 35.0 41.0 46-47 38.37102653331714 40.0 38.0 41.0 34.5 41.0 48-49 38.1650606651808 40.0 38.0 41.0 34.0 41.0 50-51 38.01455929391268 40.0 38.0 41.0 34.0 41.0 52-53 37.874990061691776 40.0 37.0 41.0 33.0 41.0 54-55 37.75694698666126 40.0 37.0 41.0 33.0 41.0 56-57 37.56893079530056 40.0 36.5 41.0 33.0 41.0 58-59 37.36593506156509 39.5 36.0 41.0 33.0 41.0 60-61 37.16906088884734 39.0 35.5 41.0 33.0 41.0 62-63 36.89708150111702 39.0 35.0 41.0 32.5 41.0 64-65 36.6028097235534 38.5 35.0 41.0 32.0 41.0 66-67 36.3497707854802 37.5 35.0 40.0 32.0 41.0 68-69 36.045621858511694 37.0 35.0 40.0 31.5 41.0 70-71 35.76314936469092 36.5 35.0 39.5 31.0 41.0 72-73 35.43182932146037 36.0 35.0 39.0 31.0 41.0 74-75 35.1020303854491 35.5 35.0 39.0 31.0 41.0 76-77 34.77256389130854 35.0 35.0 37.5 31.0 39.5 78-79 34.485152822785324 35.0 35.0 37.0 31.0 39.0 80-81 34.24099720329454 35.0 34.5 36.5 30.5 39.0 82-83 33.98686090125385 35.0 34.0 36.0 30.0 38.0 84-85 33.779187828346416 35.0 34.0 36.0 30.0 37.0 86-87 33.587611319973334 35.0 34.0 35.5 30.0 37.0 88-89 33.41607393577101 35.0 34.0 35.0 30.0 36.5 90-91 33.314125677879986 35.0 34.0 35.0 30.0 36.0 92-93 33.23952327573629 35.0 34.0 35.0 30.0 36.0 94-95 33.1734831083708 35.0 34.0 35.0 30.0 36.0 96-97 33.11761365274546 35.0 34.0 35.0 29.5 35.5 98-99 33.06159654225506 35.0 34.0 35.0 29.0 35.0 100 32.49218958185715 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33391959429825846 1101 2 0.3130099129830768 1101 3 0.2999187929773015 1101 4 0.1738883112264702 1101 5 0.18570923026332764 1101 6 0.19004472014095342 1101 7 0.21664003918812824 1101 8 0.1521925987598678 1101 9 0.24863910813463264 1101 10-11 0.29906258284910336 1101 12-13 0.29714760495024706 1101 14-15 0.38106750846286275 1101 16-17 0.43135692811331694 1101 18-19 0.41115742177242964 1101 20-21 0.38610623887640116 1101 22-23 0.41247733171940837 1101 24-25 0.38755583050499354 1101 26-27 0.4235623590913562 1101 28-29 0.4581178978601841 1101 30-31 0.46657466699261363 1101 32-33 0.48288894202843835 1101 34-35 0.47358945811029685 1101 36-37 0.3909315066339616 1101 38-39 0.4241986653994161 1101 40-41 0.47631530469099204 1101 42-43 0.4571058787469724 1101 44-45 0.4712357180939222 1101 46-47 0.5714824242379422 1101 48-49 0.4992733774940561 1101 50-51 0.4913952949460594 1101 52-53 0.5821276814564484 1101 54-55 0.6723837879085934 1101 56-57 0.5359291453076196 1101 58-59 0.5794567551288381 1101 60-61 0.630521893957237 1101 62-63 0.5978846776481177 1101 64-65 0.5727287956154967 1101 66-67 0.4792203731139182 1101 68-69 0.5414392307424833 1101 70-71 0.5640230978751788 1101 72-73 0.4831996142070025 1101 74-75 0.3915091965669433 1101 76-77 0.4292244369638638 1101 78-79 0.35361346595287557 1101 80-81 0.3813707371385391 1101 82-83 0.4251721861666695 1101 84-85 0.432488918159855 1101 86-87 0.316004094787651 1101 88-89 0.25924348612327464 1101 90-91 0.2429997399676651 1101 92-93 0.3274154999683816 1101 94-95 0.32233695565179943 1101 96-97 0.29572846348943216 1101 98-99 0.41556797921003863 1101 100 0.36282973662923723 1102 1 0.15328757838574347 1102 2 0.14062904731670045 1102 3 0.2159060930460157 1102 4 0.06890822225084747 1102 5 0.05077631077852374 1102 6 0.014308130015422194 1102 7 0.057199640668663676 1102 8 0.13336375927973876 1102 9 0.1668501192414169 1102 10-11 0.13143535817546592 1102 12-13 0.09570380087781416 1102 14-15 0.136633467609812 1102 16-17 0.18370555019613732 1102 18-19 0.15135422631460926 1102 20-21 0.15063159276083127 1102 22-23 0.14007454150337395 1102 24-25 0.15573384154064485 1102 26-27 0.1665198902421139 1102 28-29 0.19833395433130363 1102 30-31 0.1519723280417935 1102 32-33 0.01764003270683645 1102 34-35 0.06859342400765911 1102 36-37 0.1640932758364002 1102 38-39 0.16521990548636722 1102 40-41 0.15224237566628318 1102 42-43 0.10408824080916901 1102 44-45 0.11219539730694805 1102 46-47 0.026100418404382708 1102 48-49 0.09315348372846444 1102 50-51 -0.007907785226670683 1102 52-53 0.07505406978098961 1102 54-55 0.09253969682387009 1102 56-57 0.04619064992061084 1102 58-59 -0.00563654424862392 1102 60-61 -0.03833700118135397 1102 62-63 0.028129956282413104 1102 64-65 0.02196236500012816 1102 66-67 0.07239042291072906 1102 68-69 -0.06500989365714815 1102 70-71 0.06768979760985872 1102 72-73 0.026040138928522083 1102 74-75 0.0780825842405406 1102 76-77 -0.002057048433435682 1102 78-79 0.14898131595634112 1102 80-81 0.10477012044262324 1102 82-83 0.10537930068302614 1102 84-85 0.1623161807937734 1102 86-87 0.10530600172518945 1102 88-89 0.1465610401226769 1102 90-91 0.14160866353116575 1102 92-93 0.12691345496054396 1102 94-95 0.2627748849414999 1102 96-97 0.03737097464762229 1102 98-99 0.03982151397742939 1102 100 0.18375282358770306 1103 1 0.16925562312589904 1103 2 0.18920327301901807 1103 3 0.16205565938180655 1103 4 0.07945453851224471 1103 5 0.15275003081882943 1103 6 0.07716515087932407 1103 7 0.09096475748373933 1103 8 0.03893227177685077 1103 9 0.12253215081239688 1103 10-11 0.14082012444293923 1103 12-13 0.14326831461054468 1103 14-15 0.2761601948054704 1103 16-17 0.23017626375643374 1103 18-19 0.25590222172591837 1103 20-21 0.28070101127566005 1103 22-23 0.28884552488658954 1103 24-25 0.18691836547724705 1103 26-27 0.21591669898589316 1103 28-29 0.2993957748452516 1103 30-31 0.2700622274801532 1103 32-33 0.23287598725389103 1103 34-35 0.26380614755483833 1103 36-37 0.26245686712590555 1103 38-39 0.24342999441509505 1103 40-41 0.16579386487707382 1103 42-43 0.17381740272862345 1103 44-45 0.27315045630157897 1103 46-47 0.34797764178578205 1103 48-49 0.19988515827313336 1103 50-51 0.17418761436439212 1103 52-53 0.0981129720421805 1103 54-55 0.05815894979821934 1103 56-57 0.14179218337573474 1103 58-59 0.15718675419608985 1103 60-61 0.16844874251344066 1103 62-63 0.10663827513268131 1103 64-65 0.12473579434796278 1103 66-67 0.08638609825010235 1103 68-69 0.12014906136612069 1103 70-71 0.09101904562169238 1103 72-73 0.12864343126486233 1103 74-75 0.05350944079260245 1103 76-77 -0.08368730519872969 1103 78-79 0.06837907014779177 1103 80-81 0.0751597450911845 1103 82-83 0.08130110126850809 1103 84-85 0.12388591148060613 1103 86-87 0.11097237023454198 1103 88-89 0.03575086989416576 1103 90-91 0.09485344428969 1103 92-93 0.06946160634893772 1103 94-95 0.2355048193396314 1103 96-97 0.15251669776042576 1103 98-99 0.09274153128304619 1103 100 0.10616163254584166 1104 1 0.2072741622824168 1104 2 0.20654489175944946 1104 3 0.19855661084146448 1104 4 0.1381962039672402 1104 5 0.10999390504218098 1104 6 0.13521542372717477 1104 7 0.17166735819666457 1104 8 0.09983671165346664 1104 9 0.19313813036226435 1104 10-11 0.20699309467998717 1104 12-13 0.18162788709167899 1104 14-15 0.2421973875734409 1104 16-17 0.2893670588664108 1104 18-19 0.2821456792485151 1104 20-21 0.20690724974803487 1104 22-23 0.3593510384352996 1104 24-25 0.27385282561238 1104 26-27 0.29074860919217826 1104 28-29 0.40724751076633936 1104 30-31 0.3625244372150078 1104 32-33 0.2940907271847166 1104 34-35 0.2841585670262532 1104 36-37 0.36124398390852264 1104 38-39 0.30162100444600526 1104 40-41 0.24718425498613072 1104 42-43 0.18993668512160156 1104 44-45 0.19329736557315158 1104 46-47 0.13960034574554214 1104 48-49 0.21101144997128785 1104 50-51 0.0960299403939473 1104 52-53 0.16690144956682929 1104 54-55 0.11576708745952402 1104 56-57 0.21120889133802478 1104 58-59 0.14816261819310483 1104 60-61 0.07092868428555477 1104 62-63 0.15616229095798673 1104 64-65 0.15668059145962587 1104 66-67 0.27062739130263935 1104 68-69 0.13345847503043728 1104 70-71 -0.0022265445779083848 1104 72-73 0.0722317716131542 1104 74-75 0.05967326311275656 1104 76-77 -0.027237917808186296 1104 78-79 0.004723260733065615 1104 80-81 0.06315045474147496 1104 82-83 0.1223512126077324 1104 84-85 0.06743737190441834 1104 86-87 0.12659568122938225 1104 88-89 0.06265350307838702 1104 90-91 0.008246664403067427 1104 92-93 0.11328555430669951 1104 94-95 0.00804406996500262 1104 96-97 0.05548646427659065 1104 98-99 0.007077732913643331 1104 100 0.08473778768230744 1105 1 0.17462156968149856 1105 2 0.21652798296896236 1105 3 0.21175325541804568 1105 4 0.1081730647772261 1105 5 0.1745700692678014 1105 6 0.169983220911611 1105 7 0.12171777947123275 1105 8 0.10904541879738616 1105 9 0.14318534009068173 1105 10-11 0.1334426661794481 1105 12-13 0.08695973615543551 1105 14-15 0.18662740236260333 1105 16-17 0.13326744955099912 1105 18-19 0.12537867049866236 1105 20-21 0.22396517640595448 1105 22-23 0.24535279193949577 1105 24-25 0.24678310455121988 1105 26-27 0.21593845171400972 1105 28-29 0.23376882017792866 1105 30-31 0.26631193402353404 1105 32-33 0.16440106691813128 1105 34-35 0.2605444696232695 1105 36-37 0.22336146868980222 1105 38-39 0.30416976775987337 1105 40-41 0.2351505915593819 1105 42-43 0.22109490515425279 1105 44-45 0.24476540346297782 1105 46-47 0.3206865870001039 1105 48-49 0.24209078640162573 1105 50-51 0.15442628693904226 1105 52-53 0.2164554059508248 1105 54-55 0.21630333537805058 1105 56-57 0.3243111164577783 1105 58-59 0.34887241003674063 1105 60-61 0.2876060828689404 1105 62-63 0.317378294734759 1105 64-65 0.37381663897766515 1105 66-67 0.2482107991706073 1105 68-69 0.2714419532312604 1105 70-71 0.1695564496429114 1105 72-73 0.15029663039343433 1105 74-75 0.23971956557938512 1105 76-77 0.19680817269640016 1105 78-79 0.21183687560123587 1105 80-81 0.16859735744607462 1105 82-83 0.12907358086609122 1105 84-85 0.17194610168974123 1105 86-87 0.22375759775166415 1105 88-89 0.1979699929226726 1105 90-91 0.24899881654043554 1105 92-93 0.16961527090380457 1105 94-95 0.22623651544498813 1105 96-97 0.14146939009147985 1105 98-99 0.243402567960608 1105 100 0.12132492576112952 1106 1 0.10465627520255794 1106 2 0.06395688845006475 1106 3 0.10514995654357051 1106 4 0.03266598677490151 1106 5 0.03492067642942942 1106 6 0.03162918019737759 1106 7 0.06078443035144687 1106 8 -0.05052412914285043 1106 9 0.062139068529226904 1106 10-11 0.050163105437391664 1106 12-13 0.03328439187518484 1106 14-15 0.12148147637638829 1106 16-17 0.15510676816840885 1106 18-19 0.17046865325689708 1106 20-21 0.046560949584282696 1106 22-23 0.09527213305258897 1106 24-25 0.09136119937350884 1106 26-27 0.08045705135733527 1106 28-29 0.05673495824522945 1106 30-31 0.1464729928009234 1106 32-33 0.16045562636520572 1106 34-35 0.09529180011011107 1106 36-37 0.13116207104149424 1106 38-39 0.13572404231088342 1106 40-41 0.1248491937186671 1106 42-43 0.016538973026122505 1106 44-45 0.0921944939415198 1106 46-47 0.190720700529333 1106 48-49 0.15716104843200895 1106 50-51 0.24245621212607205 1106 52-53 0.2525471128852672 1106 54-55 0.2402371950907778 1106 56-57 0.28810896604745295 1106 58-59 0.3038719660428697 1106 60-61 0.3276654300499473 1106 62-63 0.2219148021309607 1106 64-65 0.24530316746356817 1106 66-67 0.1668370821279055 1106 68-69 0.19831523737994416 1106 70-71 0.08987527424313413 1106 72-73 0.06540703686982852 1106 74-75 0.1399646871967093 1106 76-77 0.26690685033464945 1106 78-79 0.23372484854721165 1106 80-81 0.13576419554371455 1106 82-83 0.10574928774133951 1106 84-85 0.20516849822066519 1106 86-87 0.23428635745478488 1106 88-89 0.15911349914228623 1106 90-91 0.10038574240085296 1106 92-93 0.2185404357365357 1106 94-95 0.13468571884597935 1106 96-97 0.13430758392440367 1106 98-99 0.1316670699104776 1106 100 0.03688210107210921 1107 1 0.11833541230442535 1107 2 0.13238785765798156 1107 3 0.15900981674955972 1107 4 0.0401771805282749 1107 5 0.01752510735629187 1107 6 0.06670470495418357 1107 7 0.04296475122140464 1107 8 0.050390777161290146 1107 9 -0.015414148109151427 1107 10-11 0.04981256189201133 1107 12-13 0.03226257596435289 1107 14-15 0.07412810660675007 1107 16-17 0.09562004013162095 1107 18-19 0.15958380866394606 1107 20-21 0.09852854503551356 1107 22-23 0.03518558213998091 1107 24-25 0.08952909332482761 1107 26-27 0.08795060326141879 1107 28-29 0.14309516246727583 1107 30-31 0.048875812674111785 1107 32-33 0.09803717581354476 1107 34-35 0.09774842762362823 1107 36-37 0.08656982710411398 1107 38-39 0.1545549522421794 1107 40-41 0.13340213867257233 1107 42-43 0.10981654635446603 1107 44-45 0.05158419180720841 1107 46-47 0.13503226264452195 1107 48-49 0.09924338647101649 1107 50-51 0.1538117412580604 1107 52-53 0.14346420677872374 1107 54-55 0.05959048017953705 1107 56-57 0.07925416905221283 1107 58-59 0.09585639181831596 1107 60-61 0.0872493646763175 1107 62-63 0.10924301462350883 1107 64-65 0.17318081771038152 1107 66-67 0.0566623219688438 1107 68-69 0.06388081742630192 1107 70-71 -0.016417749816824312 1107 72-73 -0.11017399743651168 1107 74-75 -0.04174124434585735 1107 76-77 -0.01029705768780076 1107 78-79 -0.05147593333954603 1107 80-81 -0.11708372818704049 1107 82-83 -0.0658296930537503 1107 84-85 -0.03705391610169073 1107 86-87 0.015259446577871927 1107 88-89 0.12216070520261013 1107 90-91 -0.02066998114430163 1107 92-93 -0.010947738931889717 1107 94-95 -0.07364546806235239 1107 96-97 -0.10357087784747421 1107 98-99 0.018524068608513744 1107 100 0.10094706541793386 1108 1 0.07265385800130986 1108 2 0.029934431532666395 1108 3 0.09096671382617671 1108 4 0.070948586244306 1108 5 0.016406032626036904 1108 6 -0.009367181775019162 1108 7 0.07620875920259351 1108 8 0.07870007849892602 1108 9 -0.007073577066101677 1108 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-0.4522055174481139 2214 64-65 -0.4297905923507557 2214 66-67 -0.4490487808778454 2214 68-69 -0.4210347039629596 2214 70-71 -0.39798841032392573 2214 72-73 -0.4693785232033463 2214 74-75 -0.41863410529799694 2214 76-77 -0.39193783721947995 2214 78-79 -0.43093963708003713 2214 80-81 -0.4094078253728526 2214 82-83 -0.400587227956656 2214 84-85 -0.37410083010275486 2214 86-87 -0.3583924199425965 2214 88-89 -0.2566844838422284 2214 90-91 -0.31927304651667754 2214 92-93 -0.35959208564548817 2214 94-95 -0.4196079384416507 2214 96-97 -0.4316497520152822 2214 98-99 -0.3486596353351388 2214 100 -0.3163164359508741 2215 1 -0.2837161943449331 2215 2 -0.27687171357143825 2215 3 -0.3513338114173834 2215 4 -0.2583898950858412 2215 5 -0.24543448148278202 2215 6 -0.2117650105272162 2215 7 -0.16197892786634327 2215 8 -0.2835182774182883 2215 9 -0.3009241820690747 2215 10-11 -0.2063020229917072 2215 12-13 -0.1982649885984813 2215 14-15 -0.360470150637056 2215 16-17 -0.33471264606049544 2215 18-19 -0.28888927609519754 2215 20-21 -0.32492151905164235 2215 22-23 -0.3361007841157786 2215 24-25 -0.2791913334939693 2215 26-27 -0.28828214077542214 2215 28-29 -0.3520072543080204 2215 30-31 -0.2993325002827234 2215 32-33 -0.3273228271522868 2215 34-35 -0.40975107761592255 2215 36-37 -0.4029873200444598 2215 38-39 -0.4455232280861381 2215 40-41 -0.4488146443650223 2215 42-43 -0.4500463327642379 2215 44-45 -0.47252536205788687 2215 46-47 -0.4460965926024798 2215 48-49 -0.46249603860372446 2215 50-51 -0.49243603913481593 2215 52-53 -0.638739110051695 2215 54-55 -0.6330631040941128 2215 56-57 -0.5853116057371963 2215 58-59 -0.574460734634819 2215 60-61 -0.5624626929219829 2215 62-63 -0.4685303289376037 2215 64-65 -0.5526248512902541 2215 66-67 -0.4575374391286431 2215 68-69 -0.4519411582244004 2215 70-71 -0.4811481449000752 2215 72-73 -0.4300322642429606 2215 74-75 -0.4725722347227048 2215 76-77 -0.4110759169419467 2215 78-79 -0.5163856961216737 2215 80-81 -0.5226580837308887 2215 82-83 -0.4705093908655158 2215 84-85 -0.43377723897281584 2215 86-87 -0.5265238227228295 2215 88-89 -0.44386964228549886 2215 90-91 -0.40345032767833544 2215 92-93 -0.5428750530341659 2215 94-95 -0.49562515317118283 2215 96-97 -0.5034212775539473 2215 98-99 -0.3849097324438162 2215 100 -0.3054082756233072 2216 1 -0.31247628462963206 2216 2 -0.37526042665638215 2216 3 -0.34604460403599546 2216 4 -0.17948755550182227 2216 5 -0.27909830477295827 2216 6 -0.27570406372891654 2216 7 -0.23689233193301362 2216 8 -0.3161216438006136 2216 9 -0.29985608005355857 2216 10-11 -0.3354254771962104 2216 12-13 -0.3235381251588052 2216 14-15 -0.481029776541817 2216 16-17 -0.4884992175898333 2216 18-19 -0.4348209018736142 2216 20-21 -0.4409687207511084 2216 22-23 -0.39486188637130937 2216 24-25 -0.4375298624827053 2216 26-27 -0.41586701706249585 2216 28-29 -0.3241588431122864 2216 30-31 -0.34656146059435855 2216 32-33 -0.41168518191787484 2216 34-35 -0.3306166687768908 2216 36-37 -0.3724261027501754 2216 38-39 -0.2999367386813532 2216 40-41 -0.24919142746064438 2216 42-43 -0.2883271572850816 2216 44-45 -0.2883704110157481 2216 46-47 -0.3352755261461695 2216 48-49 -0.3100761306628854 2216 50-51 -0.30129104544303686 2216 52-53 -0.309965077504863 2216 54-55 -0.3758841971071121 2216 56-57 -0.2774170663760529 2216 58-59 -0.29302369984213783 2216 60-61 -0.38856767755229527 2216 62-63 -0.40653564792066277 2216 64-65 -0.32694831458839957 2216 66-67 -0.3157599234305195 2216 68-69 -0.3135314130458937 2216 70-71 -0.2616369851553486 2216 72-73 -0.31598596828757053 2216 74-75 -0.347039868656438 2216 76-77 -0.35688397363806956 2216 78-79 -0.3153635251082605 2216 80-81 -0.34275220305372045 2216 82-83 -0.39864849722083306 2216 84-85 -0.45025757625887053 2216 86-87 -0.4230869243349602 2216 88-89 -0.3581700978333231 2216 90-91 -0.3794677255291319 2216 92-93 -0.5145338341746566 2216 94-95 -0.43539538549594425 2216 96-97 -0.5069423526009373 2216 98-99 -0.4992269267536784 2216 100 -0.6195195952312567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 19.0 9 77.0 10 162.0 11 162.0 12 124.0 13 114.0 14 138.0 15 196.0 16 342.0 17 656.0 18 1225.0 19 1892.0 20 2707.0 21 4073.0 22 6164.0 23 7452.0 24 7417.0 25 6568.0 26 5714.0 27 6074.0 28 7383.0 29 8975.0 30 11171.0 31 14020.0 32 18587.0 33 24768.0 34 36823.0 35 61942.0 36 111517.0 37 267152.0 38 379100.0 39 151484.0 40 357.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48530998658786 20.94461701114475 13.922943507341731 22.647129494925665 2 15.32531903153281 22.009306197689803 40.07375349681996 22.591621273957426 3 18.4538646830171 27.28767549366261 31.128288062542975 23.130171760777312 4 12.121940202400745 17.91490361806841 39.80679055113707 30.156365628393765 5 12.393653190972385 39.023401105115305 35.40372019361369 13.17922551029862 6 28.568957141409236 39.06001530788776 18.3964638080293 13.974563742673707 7 25.152962576918135 34.00211958995632 23.321518031804334 17.523399801321204 8 25.15051622412436 34.55342266598285 21.175542413204713 19.120518696688073 9 26.37779899891749 15.408003592643817 21.20585971389904 37.008337694539655 10-11 24.202598201406477 26.229794654893883 29.02750486867891 20.54010227502073 12-13 26.09310469254151 24.165466060786624 28.61564390189776 21.12578534477411 14-15 23.09314361276665 25.364495804284704 27.317909363463095 24.22445121948556 16-17 21.153437868318072 28.192992771901192 28.29023529545389 22.36333406432685 18-19 21.031426022728365 27.70459591057183 30.37653737983384 20.88744068686597 20-21 22.3914671214553 26.76939018540733 29.463523569298623 21.375619123838746 22-23 22.134191891171678 26.336392746525945 29.165265103031306 22.36415025927107 24-25 22.17007219361834 26.68617050554754 29.570027285570628 21.573730015263497 26-27 22.38526386972822 26.361635596530025 29.56089714746527 21.692203386276486 28-29 21.580117433722375 26.87511931435691 30.04022067859235 21.504542573328365 30-31 22.073678968337177 26.90138568532886 29.526455581184035 21.498479765149927 32-33 21.96256395044815 27.198211331466787 29.104962393139246 21.734262324945817 34-35 22.11886223634139 26.83904106746489 29.53352144326537 21.50857525292835 36-37 22.619582762695465 26.35545333219752 29.581736093364004 21.443227811743018 38-39 22.73363487754608 26.242777748168937 29.813210921643645 21.210376452641334 40-41 21.999139394124725 26.45292769818508 29.94384219022011 21.604090717470083 42-43 22.0830655797217 26.790316049009505 29.149926150241118 21.976692221027676 44-45 22.31953802152888 27.13857038824242 28.613990322007105 21.927901268221596 46-47 22.36910564204451 26.822656945887868 28.274876295095446 22.533361116972177 48-49 22.926484901542434 26.7489782046107 28.415391497474126 21.909145396372736 50-51 22.674548644360414 27.17871271903767 28.121264914132343 22.025473722469577 52-53 22.222911479941654 27.44898971078989 28.095663923372406 22.232434885896055 54-55 22.08449136467568 27.257036214454157 28.030969566018925 22.627502854851237 56-57 22.872599981564782 27.159268480103727 27.575327738405143 22.392803799926348 58-59 22.511202260154555 26.983281688842563 28.140400786877684 22.3651152641252 60-61 22.442447226453677 27.040465725645273 28.30905785879262 22.20802918910843 62-63 22.879633322659547 26.824857137989344 27.88514422936206 22.410365309989047 64-65 22.175724357512514 27.19264445428865 28.180807813388764 22.45082337481007 66-67 22.535638909725247 26.784610404276798 28.11663253969745 22.563118146300503 68-69 22.17472979703104 27.14257629902753 27.992022787044235 22.69067111689719 70-71 22.316432802698028 26.80378143184161 27.801169028605376 23.078616736854983 72-73 22.66596708225243 26.566179316708155 27.984497885082337 22.783355715957075 74-75 22.71858300684661 27.37940669702615 27.616582846078842 22.285427450048406 76-77 22.830186865831784 26.74775515482158 27.69676307173981 22.72529490760683 78-79 22.382634260988464 26.89265018424231 28.095216252640228 22.629499302129 80-81 22.198083893337227 27.365613263781164 27.954835065512047 22.481467777369566 82-83 22.498097520529743 26.88852212948393 28.094182999740514 22.519197350245815 84-85 22.7638568532886 26.975562661634168 27.304029496050887 22.95655098902635 86-87 22.40038862078501 27.199566995366343 27.787216141042787 22.612828242805865 88-89 22.910211308367977 26.463936562494126 27.930439614541662 22.695412514596228 90-91 22.530952915571998 26.5540674546835 28.112656293548355 22.80232333619615 92-93 22.54353415851143 27.223931271465652 27.622424667623658 22.610109902399262 94-95 21.901060756045332 27.24883164811661 27.758503041777587 23.091604554060467 96-97 22.07318367737429 26.88905809532671 27.62078128815609 23.41697693914291 98-99 22.329007929712482 27.048829415074493 27.4676381059555 23.15452454925753 100 22.341822528096124 27.170607215218517 27.444513272867223 23.043056983818136 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 262.0 1 169.0 2 228.5 3 337.0 4 184.0 5 115.0 6 268.0 7 683.0 8 1293.0 9 1565.0 10 1070.5 11 529.5 12 458.0 13 513.0 14 628.5 15 742.0 16 858.0 17 1022.0 18 1242.0 19 1491.5 20 1749.5 21 2020.5 22 2398.0 23 2928.5 24 3497.5 25 4258.5 26 5361.0 27 6652.0 28 8181.0 29 10306.5 30 12766.5 31 15105.5 32 18018.5 33 21386.0 34 24493.0 35 27069.0 36 29946.5 37 33551.0 38 35018.5 39 34817.0 40 34831.0 41 35028.5 42 35220.0 43 36369.0 44 38331.0 45 41111.5 46 43588.0 47 47134.0 48 60585.5 49 60127.0 50 49312.0 51 46181.5 52 41084.0 53 38542.0 54 36697.0 55 32712.5 56 28630.0 57 24651.5 58 20826.0 59 18774.0 60 15883.5 61 12064.5 62 9099.0 63 6995.5 64 5054.0 65 3431.0 66 2475.5 67 1772.0 68 1290.5 69 959.0 70 719.5 71 523.0 72 405.5 73 342.5 74 252.5 75 175.0 76 138.5 77 89.0 78 45.0 79 23.0 80 13.5 81 8.5 82 5.5 83 3.0 84 0.5 85 1.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006640100440256133 2 0.089291876972918 3 3.4947897053979644E-4 4 0.0 5 0.03730688010512327 6 0.0046305963596523034 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.3684871317474555E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.242184558096947E-4 24-25 0.0 26-27 0.0 28-29 4.3684871317474555E-5 30-31 8.736974263494911E-5 32-33 0.0019658192092863553 34-35 0.0015289704961116096 36-37 7.426428123970675E-4 38-39 6.989579410795929E-4 40-41 0.0016163402387465587 42-43 6.552730697621184E-4 44-45 4.8053358449222014E-4 46-47 2.1842435658737282E-4 48-49 6.115881984446438E-4 50-51 7.863276837145421E-4 52-53 0.0010921217829368641 54-55 0.0015726553674290842 56-57 0.0010921217829368641 58-59 0.003276365348810592 60-61 0.0021405586945562535 62-63 0.0010921217829368641 64-65 0.005591663528636743 66-67 0.005285869429414422 68-69 0.004455856874382405 70-71 0.0018347645953339317 72-73 0.006290621469716337 74-75 0.0046305963596523034 76-77 0.004368487131747457 78-79 7.426428123970675E-4 80-81 4.3684871317474555E-5 82-83 1.7473948526989822E-4 84-85 8.736974263494911E-5 86-87 4.3684871317474555E-5 88-89 1.3105461395242367E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 2.1842435658737282E-4 98-99 4.3684871317474565E-4 100 6.989579410795929E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1144561.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.91122875850786 #Duplication Level Percentage of deduplicated Percentage of total 1 75.88590565638637 50.01733287264627 2 14.436740887001054 19.0308666226086 3 4.795756102427968 9.482825326114195 4 2.077508298424621 5.477244988206544 5 1.0817074785144971 3.5648334533078865 6 0.5808073988756516 2.2969037599156192 7 0.35434299772544 1.634862766743983 8 0.21863619179964466 1.1528464042076303 9 0.1449850013994283 0.8600525634411269 >10 0.41028601938526377 3.8853833413723518 >50 0.0075679411688408995 0.34101404878017383 >100 0.004933737376364456 0.8118877440636851 >500 1.6445790299153955E-4 0.05885914998614381 >1k 6.578316119661582E-4 1.3850869586057932 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3915 0.34205254241582583 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3535 0.30885204021454515 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3351 0.2927760075697145 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1977 0.1727299811892944 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.736974263494912E-5 2 0.0 0.0 0.0 8.736974263494912E-5 8.736974263494912E-5 3 0.0 0.0 0.0 8.736974263494912E-5 8.736974263494912E-5 4 0.0 0.0 0.0 8.736974263494912E-5 8.736974263494912E-5 5 0.0 0.0 0.0 8.736974263494912E-5 8.736974263494912E-5 6 0.0 0.0 0.0 8.736974263494912E-5 8.736974263494912E-5 7 0.0 0.0 0.0 8.736974263494912E-5 8.736974263494912E-5 8 0.0 0.0 0.0 8.736974263494912E-5 8.736974263494912E-5 9 0.0 0.0 0.0 8.736974263494912E-5 8.736974263494912E-5 10-11 0.0 0.0 0.0 8.736974263494912E-5 8.736974263494912E-5 12-13 0.0 0.0 0.0 8.736974263494912E-5 1.7473948526989825E-4 14-15 0.0 0.0 0.0 8.736974263494912E-5 2.184243565873728E-4 16-17 0.0 0.0 0.0 1.310546139524237E-4 5.242184558096947E-4 18-19 0.0 0.0 0.0 2.184243565873728E-4 5.242184558096947E-4 20-21 0.0 0.0 0.0 3.0579409922232195E-4 6.115881984446439E-4 22-23 0.0 0.0 0.0 5.242184558096947E-4 7.863276837145421E-4 24-25 0.0 0.0 0.0 0.001092121782936864 7.863276837145421E-4 26-27 0.0 0.0 0.0 0.0019221343379688806 9.610671689844403E-4 28-29 0.0 0.0 0.0 0.004805335844922202 9.610671689844403E-4 30-31 0.0 0.0 0.0 0.011838600127035605 9.610671689844403E-4 32-33 0.0 0.0 0.0 0.025031431264912925 0.0010484369116193894 34-35 0.0 0.0 0.0 0.0491017953608414 0.0010484369116193894 36-37 0.0 0.0 0.0 0.08675815443650448 0.0010484369116193894 38-39 0.0 0.0 0.0 0.1354667859554886 0.0010484369116193894 40-41 0.0 0.0 0.0 0.19195132456898323 0.0010484369116193894 42-43 0.0 0.0 0.0 0.24555266167552453 0.0010484369116193894 44-45 0.0 0.0 0.0 0.3092015191850849 0.0010484369116193894 46-47 0.0 0.0 0.0 0.3836405399100616 0.0010484369116193894 48-49 0.0 0.0 0.0 0.4615743503404362 0.0011358066542543385 50-51 0.0 0.0 0.0 0.538241299502604 0.0013105461395242369 52-53 0.0 0.0 0.0 0.6118939925438662 0.0013105461395242369 54-55 0.0 0.0 0.0 0.6949826177897027 0.0013105461395242369 56-57 0.0 0.0 0.0 0.7892545700928129 0.001397915882159186 58-59 0.0 0.0 0.0 0.8981172694159595 0.001397915882159186 60-61 0.0 0.0 0.0 1.012396892782473 0.001397915882159186 62-63 0.0 0.0 0.0 1.1185948149552536 0.0015726553674290842 64-65 0.0 0.0 0.0 1.2256227496830663 0.0015726553674290842 66-67 0.0 0.0 0.0 1.344358229923962 0.0015726553674290842 68-69 0.0 0.0 0.0 1.4786892092251964 0.0015726553674290842 70-71 0.0 0.0 0.0 1.627873044774372 0.0015726553674290842 72-73 0.0 0.0 0.0 1.7777121533933098 0.0016163402387465587 74-75 0.0 0.0 0.0 1.926677564585898 0.0017473948526989824 76-77 0.0 0.0 0.0 2.0887047523024114 0.0018347645953339315 78-79 0.0 0.0 0.0 2.266764287792437 0.0018347645953339315 80-81 0.0 0.0 0.0 2.4602445828575323 0.001878449466651406 82-83 0.0 0.0 0.0 2.6572196676280253 0.0020095040806038297 84-85 0.0 0.0 0.0 2.8586506092729005 0.0020095040806038297 86-87 0.0 0.0 0.0 3.0732306971843353 0.0020095040806038297 88 0.0 0.0 0.0 3.2340783933752766 0.0020095040806038297 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 460 0.0 30.68132 1 GTTACGT 65 0.0061453036 28.9316 3 CTAACGC 260 0.0 27.123375 3 TTCGACG 35 0.008838301 26.856842 38-39 TAACGCC 295 0.0 25.49904 4 TCTAACG 300 0.0 25.087212 2 GAGTACT 4055 0.0 23.237936 12-13 GGGCTAT 840 0.0 22.380703 6 AGTACTT 4325 0.0 21.515581 12-13 GTACTTT 4645 0.0 20.235703 14-15 CATGGGG 2605 0.0 20.213251 4 GGACGCA 120 0.0050644465 19.583115 6 GCTACGC 60 0.005067244 19.582258 32-33 TAGGCAT 875 0.0 19.342842 5 TAGTACT 660 0.0 19.232939 4 TACCTGG 790 0.0 19.053577 2 CGCCTAC 380 1.2732926E-11 18.551615 7 GCTATTG 920 0.0 18.390297 8 ACTTTTT 5185 0.0 18.218864 16-17 AGGGCTA 1035 0.0 18.169605 5 >>END_MODULE