FastQCFastQC Report
Mon 16 Jan 2017
ERR1146573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001905
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG267140.6675320878431648No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA230890.576950227454175No Hit
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA58760.14683007217812516No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG55100.1376844277912644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19250.027.1136571
CATGGGG82050.021.1345944
GTGATCG16900.020.8531578
TAGGCAT29800.020.342965
TGATCGC17050.020.1185079
TACCTGG33750.019.7724592
CTAGGCA29900.019.646494
CGACGAG10450.019.5599424-25
GTACCCG4350.019.4571461
CTGTGCG26300.019.1172459
GGGCTAT27900.018.863256
GCATCGT22500.018.7956478
GTATAAG42550.018.6759641
CTATTGA28650.018.0411979
GTACATG195750.017.8717481
ATGGGGG58150.017.8600735
ACATGGG187700.017.626033
GGCTATT28550.017.6104137
TAAGGTG22200.017.5699794
TAGTACT31350.017.5385824
Produced by FastQC (version 0.11.5)