##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146573_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4001905 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.668883694140664 33.0 31.0 34.0 30.0 34.0 2 31.84693064927828 33.0 31.0 34.0 30.0 34.0 3 31.90614419882531 34.0 31.0 34.0 30.0 34.0 4 35.4026689788988 37.0 35.0 37.0 33.0 37.0 5 35.45105943294506 37.0 35.0 37.0 33.0 37.0 6 35.452678661787324 37.0 35.0 37.0 33.0 37.0 7 35.44268367190126 37.0 35.0 37.0 33.0 37.0 8 35.41904692890011 37.0 35.0 37.0 33.0 37.0 9 37.059508409120156 39.0 38.0 39.0 34.0 39.0 10-11 37.04697800172667 39.0 38.0 39.0 34.0 39.0 12-13 37.01952395171799 39.0 38.0 39.0 34.0 39.0 14-15 38.42286823400356 40.5 39.0 41.0 34.0 41.0 16-17 38.41220681150602 40.0 39.0 41.0 34.0 41.0 18-19 38.464193802701466 40.5 39.0 41.0 34.0 41.0 20-21 38.545267941143024 41.0 39.0 41.0 34.0 41.0 22-23 38.546423765681595 41.0 39.0 41.0 34.0 41.0 24-25 38.58204455128245 41.0 39.0 41.0 34.0 41.0 26-27 38.56684728897862 41.0 39.0 41.0 34.0 41.0 28-29 38.50580748418565 40.0 38.5 41.0 34.0 41.0 30-31 38.502262922283265 40.0 38.5 41.0 34.0 41.0 32-33 38.518011921822236 40.0 38.0 41.0 34.0 41.0 34-35 38.456936633928095 40.0 38.0 41.0 33.5 41.0 36-37 38.35554879488643 40.0 38.0 41.0 33.0 41.0 38-39 38.2918212701201 40.0 38.0 41.0 33.0 41.0 40-41 38.20944050395999 40.0 38.0 41.0 33.0 41.0 42-43 38.098228843513276 40.0 38.0 41.0 33.0 41.0 44-45 37.98557299586072 40.0 38.0 41.0 33.0 41.0 46-47 37.886877624531316 40.0 38.0 41.0 33.0 41.0 48-49 37.76268876947354 40.0 37.0 41.0 32.0 41.0 50-51 37.61082534442971 40.0 37.0 41.0 32.0 41.0 52-53 37.44504629670119 40.0 36.5 41.0 31.0 41.0 54-55 37.27099468877947 40.0 36.0 41.0 31.0 41.0 56-57 37.13153735533452 39.0 35.5 41.0 31.0 41.0 58-59 36.91687571294171 39.0 35.0 41.0 31.0 41.0 60-61 36.713924243578994 39.0 35.0 41.0 31.0 41.0 62-63 36.43086330135273 38.0 35.0 40.0 30.5 41.0 64-65 36.11555096885108 37.5 35.0 40.0 30.0 41.0 66-67 35.82101086857384 37.0 35.0 40.0 30.0 41.0 68-69 35.54412660970213 36.5 35.0 39.5 30.0 41.0 70-71 35.22954143089353 36.0 34.0 39.0 29.0 41.0 72-73 34.89955471206838 35.5 34.0 39.0 29.0 40.5 74-75 34.57190812875368 35.0 34.0 37.5 29.0 40.0 76-77 34.224462349805904 35.0 34.0 37.0 29.0 39.0 78-79 33.9351727489783 35.0 34.0 37.0 29.0 39.0 80-81 33.67754856749473 35.0 34.0 36.0 28.5 39.0 82-83 33.449438579876336 35.0 34.0 36.0 28.0 37.0 84-85 33.25880062120415 35.0 34.0 35.5 28.0 37.0 86-87 33.05938284392059 35.0 33.0 35.0 28.0 36.5 88-89 32.89301595115326 35.0 33.0 35.0 27.0 36.0 90-91 32.70994951154513 35.0 33.0 35.0 27.0 36.0 92-93 32.6364124085904 35.0 33.0 35.0 27.0 36.0 94-95 32.565578768111685 35.0 33.0 35.0 27.0 36.0 96-97 32.495121948172184 35.0 33.0 35.0 26.5 35.0 98-99 32.42803714730859 35.0 33.0 35.0 26.0 35.0 100 31.41463028232804 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23945148844390118 1101 2 0.281804293413348 1101 3 0.30884348651722604 1101 4 0.2916003574649224 1101 5 0.25874246749845753 1101 6 0.24595277525805415 1101 7 0.2970855726428354 1101 8 0.325634370079257 1101 9 0.3944593817010542 1101 10-11 0.39338612422820063 1101 12-13 0.3575113044439604 1101 14-15 0.4678148026799889 1101 16-17 0.46953243493477714 1101 18-19 0.4250386358982894 1101 20-21 0.41085939966511376 1101 22-23 0.4058833172530498 1101 24-25 0.42411690292055226 1101 26-27 0.3929548383038153 1101 28-29 0.45632836122658205 1101 30-31 0.4453794123648436 1101 32-33 0.4068999440656711 1101 34-35 0.360066158835302 1101 36-37 0.4057306009546906 1101 38-39 0.3968593732188026 1101 40-41 0.3696071670775609 1101 42-43 0.387913516798271 1101 44-45 0.3023575378774268 1101 46-47 0.28724563719979557 1101 48-49 0.29961228283578833 1101 50-51 0.34480683088845154 1101 52-53 0.33673560307790495 1101 54-55 0.3192487901116152 1101 56-57 0.20397069515728106 1101 58-59 0.20525010616648132 1101 60-61 0.2611124926028765 1101 62-63 0.23582838465634381 1101 64-65 0.26885578133980204 1101 66-67 0.272074569464003 1101 68-69 0.23269829353059635 1101 70-71 0.1775568406184007 1101 72-73 0.22458957307952687 1101 74-75 0.22924571343852307 1101 76-77 0.2549832952467739 1101 78-79 0.2857249099309058 1101 80-81 0.26569421582676966 1101 82-83 0.18157261742739905 1101 84-85 0.2333985034311894 1101 86-87 0.326092132294697 1101 88-89 0.31416716842029047 1101 90-91 0.327607494357153 1101 92-93 0.40124707424327966 1101 94-95 0.285101690080225 1101 96-97 0.3096761964141521 1101 98-99 0.2827700475612289 1101 100 0.277620722699929 1102 1 0.16410706862600577 1102 2 0.1508025313993535 1102 3 0.24351033535289446 1102 4 0.13003115121575348 1102 5 0.14699172913816483 1102 6 0.06509943561511733 1102 7 0.13324623349055997 1102 8 0.0974843120259763 1102 9 0.22949265371811833 1102 10-11 0.22981338609893953 1102 12-13 0.2808799016134955 1102 14-15 0.3333998365190993 1102 16-17 0.27475725661330586 1102 18-19 0.22074935849852295 1102 20-21 0.24593766864776967 1102 22-23 0.2306676197289832 1102 24-25 0.28055926440318757 1102 26-27 0.2766361118187106 1102 28-29 0.30126076937170154 1102 30-31 0.2928479000933919 1102 32-33 0.2675818572659736 1102 34-35 0.3004983774827821 1102 36-37 0.35201545691727887 1102 38-39 0.3105671894905484 1102 40-41 0.27237332663737135 1102 42-43 0.22223556193500116 1102 44-45 0.27258673405493994 1102 46-47 0.21024970005718302 1102 48-49 0.19705364151644034 1102 50-51 0.2023914784036407 1102 52-53 0.19941260713761721 1102 54-55 0.2117332629958355 1102 56-57 0.2377851404962854 1102 58-59 0.2725701034723329 1102 60-61 0.2219857225186601 1102 62-63 0.2776055261803734 1102 64-65 0.1774348159906438 1102 66-67 0.15463307816989413 1102 68-69 0.20203926313036646 1102 70-71 0.15837816545469963 1102 72-73 0.15787128749924761 1102 74-75 0.0737338228976725 1102 76-77 0.14252839690720265 1102 78-79 0.09696602003113242 1102 80-81 0.05927350436257939 1102 82-83 0.04855643963323786 1102 84-85 0.08757009947944994 1102 86-87 0.05090203265663007 1102 88-89 0.05105281043167054 1102 90-91 0.05335415171295921 1102 92-93 0.056038010009160644 1102 94-95 0.059464417010630655 1102 96-97 0.025414180675731757 1102 98-99 -0.04930088423323298 1102 100 -0.07693512518686063 1103 1 0.1388604910345741 1103 2 0.07095627391511883 1103 3 0.10497885205217727 1103 4 0.06071225638320499 1103 5 0.07570477490232719 1103 6 0.033146126998808256 1103 7 0.10394048924132449 1103 8 0.183846123556215 1103 9 0.27444578918673557 1103 10-11 0.2444216834955455 1103 12-13 0.1718225744421389 1103 14-15 0.262766859595267 1103 16-17 0.23408366891349885 1103 18-19 0.22426178890711412 1103 20-21 0.2350007419294542 1103 22-23 0.23016331878699958 1103 24-25 0.18186371286964942 1103 26-27 0.19174603484277242 1103 28-29 0.2844989891808609 1103 30-31 0.25974057129357675 1103 32-33 0.25663963392340605 1103 34-35 0.28680382046833586 1103 36-37 0.25581276678052234 1103 38-39 0.24320040187529202 1103 40-41 0.25327168479186213 1103 42-43 0.27400146476890797 1103 44-45 0.21276323253017893 1103 46-47 0.1894789568980073 1103 48-49 0.19918764104186693 1103 50-51 0.2515035232346037 1103 52-53 0.2573443829615485 1103 54-55 0.2404237472177897 1103 56-57 0.1999348404622623 1103 58-59 0.2332791631840223 1103 60-61 0.1596819053267211 1103 62-63 0.25046412799785855 1103 64-65 0.23632642321326358 1103 66-67 0.22116389230964728 1103 68-69 0.18881396625354085 1103 70-71 0.14598655723506937 1103 72-73 0.14712395915806553 1103 74-75 0.1823779926063338 1103 76-77 0.11081937690980226 1103 78-79 0.08318366368057184 1103 80-81 0.14710378938826807 1103 82-83 0.18301797608797443 1103 84-85 0.20569771912748536 1103 86-87 0.2017147674528701 1103 88-89 0.18371347386979409 1103 90-91 0.2083161373214324 1103 92-93 0.1867827286724193 1103 94-95 0.15416384210049472 1103 96-97 0.1839637256728608 1103 98-99 0.14020150975784418 1103 100 0.1243523408482794 1104 1 0.1231454781223782 1104 2 0.14173860537696825 1104 3 0.14097102958704966 1104 4 0.13463327764912236 1104 5 0.10522370081140764 1104 6 0.11126965673345524 1104 7 0.09501483500591235 1104 8 0.07184588308614792 1104 9 0.17831028365069557 1104 10-11 0.20199162238478152 1104 12-13 0.14876922210644494 1104 14-15 0.2521640001578689 1104 16-17 0.23519355589257174 1104 18-19 0.22073258028886755 1104 20-21 0.19810239084837633 1104 22-23 0.15934320967371463 1104 24-25 0.13654956290519493 1104 26-27 0.1631886216341698 1104 28-29 0.1592324219041359 1104 30-31 0.18220706721342594 1104 32-33 0.19641041792117164 1104 34-35 0.23983296733543114 1104 36-37 0.1670591368587253 1104 38-39 0.21346191634009415 1104 40-41 0.16975322054609876 1104 42-43 0.21870172177128921 1104 44-45 0.14852664494418377 1104 46-47 0.1487735170468909 1104 48-49 0.1353969522650189 1104 50-51 0.13884738105405603 1104 52-53 0.11234861079125125 1104 54-55 0.15639943000901724 1104 56-57 0.15387649118471813 1104 58-59 0.15737565245174778 1104 60-61 0.1790865899842089 1104 62-63 0.09842709168687946 1104 64-65 0.18439771278627148 1104 66-67 0.18639591488508245 1104 68-69 0.12304201323145492 1104 70-71 0.05106246866889563 1104 72-73 0.013209985364078136 1104 74-75 0.008885741558195548 1104 76-77 0.06381021519630536 1104 78-79 0.06826863848348808 1104 80-81 0.1538888189503922 1104 82-83 0.08649427965790579 1104 84-85 0.05140149757875889 1104 86-87 0.12141193689800645 1104 88-89 0.08050027428486572 1104 90-91 0.10793597570986435 1104 92-93 0.11147259253238673 1104 94-95 0.09340998641128806 1104 96-97 0.10175804502494401 1104 98-99 0.053551788716191595 1104 100 -0.003844587800070798 1105 1 0.18573698453485932 1105 2 0.1718849009513086 1105 3 0.15913423362106727 1105 4 0.08420957499995296 1105 5 0.07727298950605643 1105 6 0.10839268031456584 1105 7 8.21133100302518E-4 1105 8 0.08432743939599163 1105 9 0.14714898225891204 1105 10-11 0.15029885866080406 1105 12-13 0.14903077750032878 1105 14-15 0.21139208079052452 1105 16-17 0.13025411408349186 1105 18-19 0.14556450317880376 1105 20-21 0.16668752787910535 1105 22-23 0.12073638034939904 1105 24-25 0.1370349261704149 1105 26-27 0.14913558735235455 1105 28-29 0.14996422788308195 1105 30-31 0.17420924441761088 1105 32-33 0.1469601760294239 1105 34-35 0.13780450889336038 1105 36-37 0.18412770071435602 1105 38-39 0.22459559368475368 1105 40-41 0.17441709025047203 1105 42-43 0.18684564190998998 1105 44-45 0.10111302240255071 1105 46-47 0.13431471588541655 1105 48-49 0.18594656226931505 1105 50-51 0.1337712886563267 1105 52-53 0.14059581483530081 1105 54-55 0.20485853360637662 1105 56-57 0.17143248796348587 1105 58-59 0.15892371860515198 1105 60-61 0.23080398073874164 1105 62-63 0.1371092641547733 1105 64-65 0.10631231042160749 1105 66-67 0.13287285202223842 1105 68-69 0.16153964436555412 1105 70-71 0.15464909471406685 1105 72-73 0.13064007858527305 1105 74-75 0.11080904618746246 1105 76-77 0.07836029017108359 1105 78-79 0.07101336565966676 1105 80-81 0.11615221432714407 1105 82-83 0.07624117530394159 1105 84-85 0.14748887952376322 1105 86-87 0.1118261927929467 1105 88-89 0.11518016504034989 1105 90-91 0.16565795677579587 1105 92-93 0.16691494982490696 1105 94-95 0.15527046685902235 1105 96-97 0.10649604266090762 1105 98-99 0.09565603613274476 1105 100 0.08561719252955058 1106 1 0.05062941305292412 1106 2 0.008327561940227213 1106 3 0.07369573097540183 1106 4 0.06398845145804444 1106 5 0.07361150776085168 1106 6 0.0778438877424108 1106 7 0.02750443781688716 1106 8 0.018492429688514278 1106 9 0.1142661507270546 1106 10-11 0.07961709030384867 1106 12-13 0.06046732637357621 1106 14-15 0.15269386496706971 1106 16-17 0.09129908417182975 1106 18-19 0.047203076328642624 1106 20-21 0.017998765227375202 1106 22-23 0.053821236157837404 1106 24-25 0.046426801353469216 1106 26-27 0.0789276877278482 1106 28-29 0.1260037347747769 1106 30-31 0.0406958389782659 1106 32-33 0.05288182519574036 1106 34-35 0.016178405511794836 1106 36-37 0.08493585043576246 1106 38-39 0.03743756085646055 1106 40-41 0.0535370683626013 1106 42-43 -0.01635475656136265 1106 44-45 -0.06554227356591014 1106 46-47 -0.04398132798852572 1106 48-49 -0.042724591877011164 1106 50-51 -0.05852891882237543 1106 52-53 -0.0022211952615336372 1106 54-55 0.03686481584405499 1106 56-57 -0.04076092848994506 1106 58-59 0.0017944486741328092 1106 60-61 0.014574884262017918 1106 62-63 0.042361912884388175 1106 64-65 0.03236554984291473 1106 66-67 0.03674954688472809 1106 68-69 0.022696035604091946 1106 70-71 -0.04087350432939729 1106 72-73 -0.026518831160615264 1106 74-75 0.011361311353311976 1106 76-77 -0.0014746914750318751 1106 78-79 -0.008627659351780892 1106 80-81 -0.007721551630567092 1106 82-83 -0.016535794735297316 1106 84-85 0.06619623282860232 1106 86-87 0.0015558390970014102 1106 88-89 -0.02750627202591005 1106 90-91 0.02690777481539186 1106 92-93 0.025653556473002936 1106 94-95 0.002085852904457397 1106 96-97 0.03036684463712902 1106 98-99 -0.0033560635762981406 1106 100 0.008959791958851326 1107 1 0.14396962684802972 1107 2 0.11454721632766507 1107 3 0.20408383474408964 1107 4 0.1452548918968688 1107 5 0.15710395177272574 1107 6 0.12337551772598232 1107 7 0.10067311908711929 1107 8 0.12266238144907504 1107 9 0.16067138161989192 1107 10-11 0.24457552991962928 1107 12-13 0.19211749408483314 1107 14-15 0.2434910078335477 1107 16-17 0.17272807552008373 1107 18-19 0.13637559193216475 1107 20-21 0.18885420694321908 1107 22-23 0.17321888003515795 1107 24-25 0.2117445382476646 1107 26-27 0.26908720121823393 1107 28-29 0.22091419623384922 1107 30-31 0.19397301816313473 1107 32-33 0.18650458428268735 1107 34-35 0.24501559658504846 1107 36-37 0.19640762693397562 1107 38-39 0.2597664120915155 1107 40-41 0.21857261922957605 1107 42-43 0.17059152684615952 1107 44-45 0.22226346878910874 1107 46-47 0.24136047963506968 1107 48-49 0.2095040245296147 1107 50-51 0.25729508723939176 1107 52-53 0.1496493976230724 1107 54-55 0.17122524317299792 1107 56-57 0.1873181162580977 1107 58-59 0.13514805324136603 1107 60-61 0.20288036370065043 1107 62-63 0.24609450896318208 1107 64-65 0.29013614958619627 1107 66-67 0.2927357445913188 1107 68-69 0.3037330365937052 1107 70-71 0.27158569464115345 1107 72-73 0.3077162781374767 1107 74-75 0.22210802653159334 1107 76-77 0.2137263497391544 1107 78-79 0.22143465672922957 1107 80-81 0.2093349606938375 1107 82-83 0.20613713402252642 1107 84-85 0.17801747581886218 1107 86-87 0.1627325940230122 1107 88-89 0.13984111399739163 1107 90-91 0.12847240488714817 1107 92-93 0.1149517820323922 1107 94-95 0.21225598103128362 1107 96-97 0.15308520403892345 1107 98-99 0.1693307785203828 1107 100 0.21779267185669227 1108 1 0.14295735529574216 1108 2 0.22435454891963857 1108 3 0.14083340870799077 1108 4 0.16059017282378818 1108 5 0.13672352264152465 1108 6 0.11247076753054586 1108 7 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-0.22641879440615043 2214 62-63 -0.2565532162323052 2214 64-65 -0.23468624569429863 2214 66-67 -0.1683991444835371 2214 68-69 -0.1904316932760608 2214 70-71 -0.2799085078399415 2214 72-73 -0.18682247310575661 2214 74-75 -0.17506092970123888 2214 76-77 -0.17209273557783433 2214 78-79 -0.11539829452080141 2214 80-81 -0.1457262706518847 2214 82-83 -0.13289417779652268 2214 84-85 -0.10329162559607141 2214 86-87 -0.14584819516220193 2214 88-89 -0.22670292487308785 2214 90-91 -0.1503368606091442 2214 92-93 -0.20482742971571355 2214 94-95 -0.166614411881163 2214 96-97 -0.1933141912501739 2214 98-99 -0.14531873571917941 2214 100 -0.09372934690766854 2215 1 -0.3152804592926124 2215 2 -0.2926053689822794 2215 3 -0.30846080538074006 2215 4 -0.2002281299782993 2215 5 -0.28542936459014356 2215 6 -0.2056741143205798 2215 7 -0.19479367001538606 2215 8 -0.2270727373974779 2215 9 -0.3146239267170188 2215 10-11 -0.2688607161569223 2215 12-13 -0.34090625531927543 2215 14-15 -0.4181252193481839 2215 16-17 -0.41427677991082135 2215 18-19 -0.515412448506396 2215 20-21 -0.5160596534727233 2215 22-23 -0.3482569829013684 2215 24-25 -0.38957596021811725 2215 26-27 -0.3800467650061634 2215 28-29 -0.3500864583715071 2215 30-31 -0.358992856588479 2215 32-33 -0.42415401520070617 2215 34-35 -0.4420921193505407 2215 36-37 -0.34867995273950214 2215 38-39 -0.43671401995225523 2215 40-41 -0.40263553531912777 2215 42-43 -0.41046485921979325 2215 44-45 -0.3408826945691317 2215 46-47 -0.33576232335497735 2215 48-49 -0.33329873679948463 2215 50-51 -0.35556028394470474 2215 52-53 -0.4057345037443625 2215 54-55 -0.43201461917987416 2215 56-57 -0.3805366735957918 2215 58-59 -0.3665920512794827 2215 60-61 -0.34715775631343604 2215 62-63 -0.3967375200656349 2215 64-65 -0.3838410004302446 2215 66-67 -0.3775850289738898 2215 68-69 -0.3976205627509728 2215 70-71 -0.4007669709749635 2215 72-73 -0.33846994151979004 2215 74-75 -0.3794659594389742 2215 76-77 -0.4172285841448584 2215 78-79 -0.3839883282415357 2215 80-81 -0.3319861603988272 2215 82-83 -0.304347331118727 2215 84-85 -0.3221704558505891 2215 86-87 -0.28505577805967164 2215 88-89 -0.3179504156931259 2215 90-91 -0.37766169835752805 2215 92-93 -0.4183821583974634 2215 94-95 -0.44868804205759005 2215 96-97 -0.36627468044896716 2215 98-99 -0.3945669526822755 2215 100 -0.3695313857477345 2216 1 -0.1524071289071358 2216 2 -0.24341288349879164 2216 3 -0.1847472995401631 2216 4 -0.15773962238894512 2216 5 -0.1405119551742473 2216 6 -0.10243943508415754 2216 7 -0.19289980369551074 2216 8 -0.15400505845666146 2216 9 -0.15781415869643212 2216 10-11 -0.218177146364809 2216 12-13 -0.25369005302911063 2216 14-15 -0.36171077144901176 2216 16-17 -0.3795120418281499 2216 18-19 -0.42871816506755067 2216 20-21 -0.38442186067440076 2216 22-23 -0.37393091858681515 2216 24-25 -0.42592958071173115 2216 26-27 -0.4422697240075806 2216 28-29 -0.41418741300661566 2216 30-31 -0.4543846894933594 2216 32-33 -0.4520385307523469 2216 34-35 -0.4480358647031153 2216 36-37 -0.4291552077724319 2216 38-39 -0.44947171105015116 2216 40-41 -0.4860327437187806 2216 42-43 -0.41517755278798063 2216 44-45 -0.3705803293234311 2216 46-47 -0.32465700408111076 2216 48-49 -0.36749987214036395 2216 50-51 -0.408364077385464 2216 52-53 -0.34644849524126897 2216 54-55 -0.3867068308940276 2216 56-57 -0.4298984752947632 2216 58-59 -0.40251060825267615 2216 60-61 -0.43839621063930423 2216 62-63 -0.4192121588091453 2216 64-65 -0.42056533481333247 2216 66-67 -0.4784284425824481 2216 68-69 -0.4272528942994782 2216 70-71 -0.49643698766785604 2216 72-73 -0.47324785627299804 2216 74-75 -0.4855057757897967 2216 76-77 -0.3946743985881298 2216 78-79 -0.4515962244013707 2216 80-81 -0.41360179091752514 2216 82-83 -0.33044033293965214 2216 84-85 -0.3752821672767084 2216 86-87 -0.4049847120610224 2216 88-89 -0.43291633939606555 2216 90-91 -0.3779820995044503 2216 92-93 -0.3993512718415957 2216 94-95 -0.4102392042485974 2216 96-97 -0.4190041684039514 2216 98-99 -0.4516612994438276 2216 100 -0.47016220551335053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 109.0 1 58.0 2 54.0 3 46.0 4 41.0 5 68.0 6 150.0 7 689.0 8 2125.0 9 2963.0 10 3152.0 11 3552.0 12 4448.0 13 5230.0 14 6482.0 15 7165.0 16 7862.0 17 8550.0 18 9591.0 19 10800.0 20 12116.0 21 13648.0 22 15715.0 23 18771.0 24 23353.0 25 29012.0 26 32752.0 27 32503.0 28 35266.0 29 44598.0 30 52009.0 31 59952.0 32 77306.0 33 104694.0 34 159373.0 35 251673.0 36 473478.0 37 996593.0 38 1138306.0 39 357092.0 40 560.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42710884448142 19.182133063898103 12.737315403989072 23.653442687631408 2 14.27635705699627 21.049159188202395 40.42849038733072 24.245993367470618 3 18.992519381307623 27.200282300700728 30.48275269174068 23.324445626250967 4 12.216496867798792 17.104440111068378 39.711618071127845 30.967444950004985 5 12.80944995394082 39.52519809445455 34.99920277384718 12.666149177757442 6 28.87162045817544 37.68367665960329 19.657129404684817 13.787573477536446 7 25.94652041874272 33.78034174215582 22.70442044795465 17.56871739114681 8 24.47019804662237 34.77615923696124 21.626895077172513 19.12674763924388 9 26.373571450705363 17.167831363960325 20.750762442735578 35.707834742598735 10-11 23.961723497199074 25.986278805368677 28.58370217358247 21.468295523849783 12-13 26.482861492138298 23.505324620752983 27.87143991854353 22.14037396856519 14-15 24.313974075280576 24.795859422179923 25.961118543795337 24.929047958744167 16-17 22.06463532638793 27.977811298574334 27.668139615783698 22.28941375925404 18-19 21.634976779241864 29.019412773716063 28.085721882690688 21.259888564351385 20-21 22.814015361515143 28.426012592139166 26.194243411455375 22.565728634890313 22-23 22.85236179791959 27.503690211637107 25.274907335518513 24.36904065492479 24-25 22.89600227500412 27.898612382429594 26.083069636266725 23.122315706299567 26-27 23.78399355972987 26.869298276193177 26.649428973293944 22.697279190783007 28-29 22.87975394091498 27.020043404698647 27.391566033980315 22.708636620406057 30-31 22.606655347674167 28.129174961402303 27.069106497658147 22.195063193265383 32-33 22.51264846088005 28.523831837010107 26.2850823758739 22.67843732623595 34-35 22.965915341532593 27.90435093150705 26.488953043907674 22.640780683052686 36-37 23.595321823629536 26.89666496674914 26.574459574307497 22.93355363531383 38-39 24.227851565453246 26.445697276350653 26.96998306115168 22.35646809704442 40-41 23.42630523598955 26.380426439946486 28.08007801712148 22.11319030694249 42-43 23.021051200326326 27.041022146950493 27.113093214747035 22.824833437976142 44-45 22.385303145678417 28.090123549827666 27.024592363303658 22.499980941190262 46-47 22.557962704302973 27.80167651666868 25.741899618964393 23.89846116006395 48-49 23.150799006105668 27.346166906608254 26.101274387898993 23.401759699387085 50-51 23.581204575699918 27.06155958138405 26.04507760312672 23.312158239789312 52-53 22.85345097691725 27.37634169663768 26.700167709345795 23.07003961709928 54-55 22.729592375582456 27.173998768376396 27.06860958394145 23.0277992720997 56-57 23.022661789034927 27.534821121005365 26.620252670923346 22.82226441903636 58-59 22.234659830037508 27.45632199883412 26.982445808081767 23.326572363046605 60-61 22.864481454863604 27.01544230228416 27.085835702902855 23.03424053994938 62-63 23.979007991361232 26.799956006269106 26.21331460266912 23.007721399700543 64-65 22.8271031424839 26.830486748293826 27.06787159295942 23.274538516262854 66-67 23.15355213447287 26.396760516254602 27.345717874292525 23.103969474980005 68-69 23.122361128818163 27.24407480457667 26.701464143849574 22.9320999227556 70-71 22.620811921910327 27.295550569538747 26.408829241117882 23.674808267433043 72-73 22.753246545477634 26.88489991549689 26.604356090929393 23.75749744809608 74-75 22.895921482399185 27.86717882492577 26.38833685054668 22.848562842128366 76-77 23.445810214511337 26.578987628076355 26.218606481236133 23.756595676176172 78-79 22.705732690426615 26.91481519348321 26.80888757123398 23.570564544856193 80-81 22.783817924147893 27.608189533518374 26.982767521153427 22.625225021180302 82-83 23.212342352442835 26.654950161105077 27.11787337049768 23.014834115954407 84-85 22.97527302905394 26.759584450904768 26.661533962089646 23.60360855795165 86-87 23.006777057988938 27.30767923258667 27.133490944758677 22.55205276466571 88-89 23.7981173012318 26.32128859382499 26.938227383618624 22.94236672132459 90-91 23.18769085711836 26.152454225849848 27.28736393862976 23.372490978402034 92-93 23.189916121409794 27.088068957647504 26.795241926321673 22.926772994621025 94-95 22.77294313438628 27.071226848134007 27.05472830206838 23.10110171541133 96-97 22.992591848245937 26.30001983489419 26.53902593546773 24.168362381392143 98-99 22.668938805964554 27.02638456145087 26.867597268933558 23.43707936365102 100 22.871880138114516 27.592284223560753 26.11145743610304 23.42437820222169 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1487.0 1 1045.0 2 604.0 3 613.5 4 598.5 5 494.0 6 396.0 7 395.0 8 416.5 9 437.5 10 401.0 11 332.5 12 331.0 13 346.5 14 396.0 15 451.0 16 517.0 17 610.5 18 795.0 19 1099.0 20 1521.5 21 2156.5 22 3289.5 23 4886.0 24 6912.0 25 9670.0 26 13503.5 27 18290.5 28 23677.5 29 30613.0 30 39084.5 31 46904.5 32 55301.0 33 63590.0 34 70712.0 35 77835.0 36 85815.5 37 97431.5 38 103711.0 39 104157.0 40 105132.0 41 105621.5 42 107038.5 43 118061.0 44 149717.5 45 176944.0 46 174900.0 47 178866.5 48 254815.5 49 255584.0 50 186114.5 51 171638.0 52 155479.5 53 146394.0 54 137643.5 55 122083.0 56 110267.0 57 95260.0 58 78762.5 59 70033.5 60 58551.5 61 44609.5 62 33653.5 63 25984.0 64 19266.0 65 12788.5 66 9024.0 67 6702.5 68 5008.0 69 3769.5 70 2837.0 71 2197.5 72 1749.0 73 1396.5 74 1068.0 75 785.0 76 575.5 77 411.5 78 291.0 79 219.5 80 150.0 81 97.0 82 62.5 83 47.5 84 39.5 85 29.5 86 19.0 87 16.0 88 14.5 89 13.0 90 11.5 91 13.0 92 10.5 93 7.5 94 7.5 95 6.0 96 3.5 97 3.0 98 3.5 99 6.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.060671105386059895 2 0.010370061258325722 3 0.012444073509990867 4 0.010145168363566852 5 0.013268680790773396 6 0.012793906901837999 7 0.0028986195324476717 8 0.005522369971301167 9 0.007321513129372136 10-11 0.007134102383739744 12-13 0.00645942369946313 14-15 0.010857329196969943 16-17 0.010220132661819809 18-19 0.007821275117725183 20-21 0.007034149986069134 22-23 0.002548786140600539 24-25 0.0038481673103184606 26-27 0.005235006827998165 28-29 0.006609352295969045 30-31 0.00927058488394902 32-33 0.010744882749590507 34-35 0.01214421631697904 36-37 0.018253806624595036 38-39 0.018678604314695126 40-41 0.018291288773721513 42-43 0.02481318272172878 44-45 0.02824904639165597 46-47 0.03071037418429473 48-49 0.03194728510546852 50-51 0.03230961254702448 52-53 0.030822820631674165 54-55 0.03734471457968142 56-57 0.04005592336649671 58-59 0.03730723243055495 60-61 0.03823179210900809 62-63 0.03335911272256588 64-65 0.035957875062001717 66-67 0.037844476568034476 68-69 0.04035578055950854 70-71 0.04014338171445849 72-73 0.03649511919948125 74-75 0.03945620898047305 76-77 0.0389064707932847 78-79 0.03828176830784339 80-81 0.04312945959486794 82-83 0.03911886963833474 84-85 0.035358160675978065 86-87 0.03370894611441301 88-89 0.03160994576333022 90-91 0.034146237854221925 92-93 0.03549559522277515 94-95 0.038794024345905266 96-97 0.034471083146651406 98-99 0.031734886260418475 100 0.029161112020400285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4001905.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.82807736931454 #Duplication Level Percentage of deduplicated Percentage of total 1 71.47396374563422 39.18780014137216 2 14.434051625469888 15.827825985478869 3 5.78126051123543 9.509261958065371 4 2.886348046837983 6.330116561072114 5 1.6164454623911961 4.431329843763096 6 1.000045682239475 3.2898349223204932 7 0.6738949124645821 2.5863853679576896 8 0.48237493512629304 2.1158152211297985 9 0.332617835734124 1.6413116788840059 >10 1.294628466668138 11.056594561427938 >50 0.020475697940223497 0.7192985520259746 >100 0.003115882069900066 0.2553111850291952 >500 1.5543923770232335E-4 0.07265375454702147 >1k 3.108784754046467E-4 0.36906402822976714 >5k 1.5543923770232335E-4 0.48518219898673687 >10k+ 1.5543923770232335E-4 2.1222140397098594 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 26714 0.6675320878431648 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 23089 0.576950227454175 No Hit GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA 5876 0.14683007217812516 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5510 0.1376844277912644 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.4988099417652342E-5 0.0 10-11 0.0 0.0 0.0 3.748214912647851E-5 0.0 12-13 0.0 0.0 0.0 6.247024854413085E-5 0.0 14-15 0.0 0.0 0.0 1.1244644737943554E-4 0.0 16-17 0.0 0.0 0.0 1.749166959235664E-4 0.0 18-19 0.0 0.0 0.0 1.9990479534121874E-4 0.0 20-21 0.0 0.0 0.0 2.9985719301182814E-4 0.0 22-23 0.0 0.0 0.0 6.621846345677871E-4 0.0 24-25 0.0 0.0 0.0 0.0010744882749590507 0.0 26-27 0.0 0.0 0.0 0.002248928947588711 0.0 28-29 0.0 0.0 0.0 0.006022131959654215 0.0 30-31 0.0 0.0 0.0 0.016454663466524067 0.0 32-33 0.0 0.0 0.0 0.03891896484299352 0.0 34-35 0.0 0.0 0.0 0.07893740606036376 0.0 36-37 0.0 0.0 0.0 0.1418324522945947 0.0 38-39 0.0 0.0 0.0 0.2227938944077883 0.0 40-41 0.0 0.0 0.0 0.30363039602389363 0.0 42-43 0.0 0.0 0.0 0.37740775955451217 0.0 44-45 0.0 0.0 0.0 0.46621546488484855 0.0 46-47 0.0 0.0 0.0 0.5779372573811723 0.0 48-49 0.0 0.0 0.0 0.6977302059893975 0.0 50-51 0.0 0.0 0.0 0.8157739876383873 0.0 52-53 0.0 0.0 0.0 0.930506846114538 0.0 54-55 0.0 0.0 0.0 1.0479009371786687 0.0 56-57 0.0 0.0 0.0 1.1810375308759204 0.0 58-59 0.0 0.0 0.0 1.3423482066665748 0.0 60-61 0.0 0.0 0.0 1.5189141171517064 0.0 62-63 0.0 0.0 0.0 1.6959298134263556 0.0 64-65 0.0 0.0 0.0 1.8797297786928975 0.0 66-67 0.0 0.0 0.0 2.070413965349003 0.0 68-69 0.0 0.0 0.0 2.264459051376782 0.0 70-71 0.0 0.0 0.0 2.4744465448330235 0.0 72-73 0.0 0.0 0.0 2.688594556842304 0.0 74-75 0.0 0.0 0.0 2.8984196276523306 0.0 76-77 0.0 0.0 0.0 3.1229377009199366 0.0 78-79 0.0 0.0 0.0 3.3642352829464963 0.0 80-81 0.0 0.0 0.0 3.6217876236442397 0.0 82-83 0.0 0.0 0.0 3.8820386790790886 0.0 84-85 0.0 0.0 0.0 4.172587805057841 0.0 86-87 0.0 0.0 0.0 4.475381099751244 0.0 88 0.0 0.0 0.0 4.690241272593927 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 1925 0.0 27.113657 1 CATGGGG 8205 0.0 21.134594 4 GTGATCG 1690 0.0 20.853157 8 TAGGCAT 2980 0.0 20.34296 5 TGATCGC 1705 0.0 20.118507 9 TACCTGG 3375 0.0 19.772459 2 CTAGGCA 2990 0.0 19.64649 4 CGACGAG 1045 0.0 19.55994 24-25 GTACCCG 435 0.0 19.457146 1 CTGTGCG 2630 0.0 19.117245 9 GGGCTAT 2790 0.0 18.86325 6 GCATCGT 2250 0.0 18.795647 8 GTATAAG 4255 0.0 18.675964 1 CTATTGA 2865 0.0 18.041197 9 GTACATG 19575 0.0 17.871748 1 ATGGGGG 5815 0.0 17.860073 5 ACATGGG 18770 0.0 17.62603 3 GGCTATT 2855 0.0 17.610413 7 TAAGGTG 2220 0.0 17.569979 4 TAGTACT 3135 0.0 17.538582 4 >>END_MODULE