FastQCFastQC Report
Mon 16 Jan 2017
ERR1146573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4001905
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA299780.7490932443423819No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106870.2670478184764506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95200.2378867064560503No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70450.17604116039736076No Hit
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA57180.1428819524701361No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52310.13071274805373942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52300.13068775995432175No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT45190.11292122126837092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT114350.022.21390712-13
CTAACGC9950.021.7370683
TCTAACG10650.021.6512012
GTACCCG3550.021.2111991
CGCCTAC11150.020.6531647
AGTACTT124650.020.58570712-13
CATGGGG82850.020.5436134
CTGTGCG23250.020.415689
TACCTGG31700.020.1890182
TAACGCC11500.020.0336024
GTACTTT131100.019.3040514-15
CTAGTAC26550.019.1260593
TAGTACT26400.019.056394
GGCGTCG15550.018.738158
CTATTGA27800.018.5957329
TAGGCAT30150.018.557545
ATTTAGG31650.018.4383281
CTAGGCA31750.018.3627954
GCTATTG27450.018.3192148
GGGCTAT28750.018.3088846
Produced by FastQC (version 0.11.5)