##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146573_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4001905
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.533908476088264	34.0	31.0	34.0	31.0	34.0
2	32.59589045716977	34.0	31.0	34.0	31.0	34.0
3	32.73124049671344	34.0	31.0	34.0	31.0	34.0
4	36.16458811491027	37.0	37.0	37.0	35.0	37.0
5	36.19076764690816	37.0	37.0	37.0	35.0	37.0
6	36.27642810111684	37.0	37.0	37.0	35.0	37.0
7	36.26425839693846	37.0	37.0	37.0	35.0	37.0
8	36.278534348016755	37.0	37.0	37.0	35.0	37.0
9	38.10227279258253	39.0	39.0	39.0	37.0	39.0
10-11	38.07048168309842	39.0	38.0	39.0	36.0	39.0
12-13	38.04906488284954	39.0	38.0	39.0	35.0	39.0
14-15	39.479060847271484	41.0	39.0	41.0	37.0	41.0
16-17	39.458253506767406	41.0	39.0	41.0	36.5	41.0
18-19	39.53230386528416	41.0	39.5	41.0	37.0	41.0
20-21	39.57053390822621	41.0	40.0	41.0	37.0	41.0
22-23	39.538373974394695	41.0	39.5	41.0	37.0	41.0
24-25	39.52474109205491	41.0	39.0	41.0	37.0	41.0
26-27	39.418966966981976	41.0	39.0	41.0	36.0	41.0
28-29	39.31396022644216	41.0	39.0	41.0	36.0	41.0
30-31	39.26298825184506	41.0	39.0	41.0	36.0	41.0
32-33	39.18542681547913	41.0	39.0	41.0	35.0	41.0
34-35	39.12545137878085	41.0	39.0	41.0	35.0	41.0
36-37	39.045628394477134	41.0	39.0	41.0	35.0	41.0
38-39	38.93539027038373	40.5	39.0	41.0	35.0	41.0
40-41	38.8351385152821	40.0	38.0	41.0	35.0	41.0
42-43	38.77561998598168	40.0	38.0	41.0	35.0	41.0
44-45	38.67021506007764	40.0	38.0	41.0	35.0	41.0
46-47	38.489869199793596	40.0	38.0	41.0	35.0	41.0
48-49	38.30388664898342	40.0	38.0	41.0	34.0	41.0
50-51	38.14404527343852	40.0	38.0	41.0	34.0	41.0
52-53	37.965312769793385	40.0	37.0	41.0	34.0	41.0
54-55	37.831023724950995	40.0	37.0	41.0	33.5	41.0
56-57	37.63791681711585	40.0	36.5	41.0	33.0	41.0
58-59	37.4481570651977	39.5	36.0	41.0	33.0	41.0
60-61	37.248526014485606	39.0	35.5	41.0	33.0	41.0
62-63	36.95050207338755	39.0	35.0	41.0	32.5	41.0
64-65	36.62528908107514	38.5	35.0	41.0	32.0	41.0
66-67	36.34420432269132	37.5	35.0	40.0	32.0	41.0
68-69	36.04225350176978	37.0	35.0	40.0	32.0	41.0
70-71	35.7251060432469	36.5	35.0	39.0	31.0	41.0
72-73	35.390936566460226	36.0	35.0	39.0	31.0	41.0
74-75	35.05838831756376	35.5	35.0	38.0	31.0	40.0
76-77	34.70849982695741	35.0	35.0	37.0	31.0	39.0
78-79	34.41560719207477	35.0	34.5	37.0	31.0	39.0
80-81	34.19017780282141	35.0	34.0	36.0	31.0	39.0
82-83	33.94845154994934	35.0	34.0	36.0	30.0	37.0
84-85	33.77090835489598	35.0	34.0	36.0	30.0	37.0
86-87	33.58924737093959	35.0	34.0	35.0	30.0	37.0
88-89	33.41432018001427	35.0	34.0	35.0	30.0	36.0
90-91	33.31520825706757	35.0	34.0	35.0	30.0	36.0
92-93	33.26054891357991	35.0	34.0	35.0	30.0	36.0
94-95	33.188227231780864	35.0	34.0	35.0	30.0	36.0
96-97	33.13672513465462	35.0	34.0	35.0	29.5	35.5
98-99	33.087499953147315	35.0	34.0	35.0	29.0	35.0
100	32.475828636611816	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3136193485722245
1101	2	0.30917985715176854
1101	3	0.317968049157173
1101	4	0.2152210352374766
1101	5	0.2386737103557124
1101	6	0.2098362614324003
1101	7	0.23297020857197737
1101	8	0.22468069656083856
1101	9	0.26218231073031717
1101	10-11	0.2548677397258814
1101	12-13	0.2956861532026238
1101	14-15	0.4390572623260951
1101	16-17	0.3707474353834854
1101	18-19	0.38455416061651704
1101	20-21	0.3765997530429317
1101	22-23	0.3530693262224034
1101	24-25	0.3552911105648846
1101	26-27	0.37426152464023943
1101	28-29	0.4021595831482969
1101	30-31	0.430280133708834
1101	32-33	0.41949613594019297
1101	34-35	0.4217557034804429
1101	36-37	0.4114018959548744
1101	38-39	0.4356723919918295
1101	40-41	0.37451836641356095
1101	42-43	0.3673106325474862
1101	44-45	0.40445710182886074
1101	46-47	0.43916366815602714
1101	48-49	0.3789569845577887
1101	50-51	0.36244900978383043
1101	52-53	0.365608403744929
1101	54-55	0.34229464533860465
1101	56-57	0.3912494660496151
1101	58-59	0.4318324350125522
1101	60-61	0.3199104507832189
1101	62-63	0.2517410547258976
1101	64-65	0.30377595190906703
1101	66-67	0.3035878697900358
1101	68-69	0.30145246192714126
1101	70-71	0.29857355961770793
1101	72-73	0.28963554046768536
1101	74-75	0.28753049977635925
1101	76-77	0.2513838621961355
1101	78-79	0.25541660383081677
1101	80-81	0.2890440962694214
1101	82-83	0.32178631046816975
1101	84-85	0.3240863487460004
1101	86-87	0.3561715907011944
1101	88-89	0.3341722836054757
1101	90-91	0.3155456657320954
1101	92-93	0.3098993792329736
1101	94-95	0.3184555288330486
1101	96-97	0.2805994436163388
1101	98-99	0.29303344925448016
1101	100	0.33818648653775085
1102	1	0.27081499808324594
1102	2	0.2879770601246818
1102	3	0.24034237261044922
1102	4	0.20969240661062827
1102	5	0.2019556629561734
1102	6	0.17392667623680325
1102	7	0.18851111266767617
1102	8	0.1673895915383099
1102	9	0.2239720130166063
1102	10-11	0.23714805245631965
1102	12-13	0.24490420932672663
1102	14-15	0.31510303281199725
1102	16-17	0.3093722504662395
1102	18-19	0.29062405279131553
1102	20-21	0.3245989650398471
1102	22-23	0.3488903230149063
1102	24-25	0.315721808519946
1102	26-27	0.3222221086070647
1102	28-29	0.3446454127030165
1102	30-31	0.35965572763388565
1102	32-33	0.3196597565107808
1102	34-35	0.31321510491915916
1102	36-37	0.3729150512092758
1102	38-39	0.3718638156705154
1102	40-41	0.37895777619994675
1102	42-43	0.3427882241894906
1102	44-45	0.367490796813037
1102	46-47	0.3694460065569416
1102	48-49	0.3261853590553869
1102	50-51	0.27645265438536626
1102	52-53	0.30595383474040005
1102	54-55	0.27490816681552843
1102	56-57	0.3344633172803526
1102	58-59	0.3069331165409963
1102	60-61	0.2794310889261382
1102	62-63	0.29134671641293153
1102	64-65	0.30214552623035473
1102	66-67	0.28745677388991453
1102	68-69	0.3232692505109327
1102	70-71	0.21611833236188005
1102	72-73	0.22791327037666065
1102	74-75	0.21730962683784583
1102	76-77	0.21454652812435882
1102	78-79	0.1767818872344904
1102	80-81	0.1745401019871622
1102	82-83	0.1856532803310671
1102	84-85	0.21430738483395118
1102	86-87	0.18541918647166256
1102	88-89	0.2029193630559405
1102	90-91	0.21017459410023775
1102	92-93	0.21792056492984813
1102	94-95	0.21074875916063007
1102	96-97	0.22048308316460918
1102	98-99	0.21355977808488547
1102	100	0.1964236227711993
1103	1	0.19174370942577923
1103	2	0.1951220442256698
1103	3	0.19977637908171886
1103	4	0.1329408732067492
1103	5	0.08238642681330077
1103	6	0.08250195659647375
1103	7	0.11896743325609549
1103	8	0.12607637933967197
1103	9	0.17242946155751326
1103	10-11	0.15971090739245142
1103	12-13	0.16609619405118536
1103	14-15	0.2113469618420467
1103	16-17	0.21569530233876577
1103	18-19	0.2139366812396446
1103	20-21	0.21342211578268433
1103	22-23	0.21698830233821553
1103	24-25	0.23348932834053926
1103	26-27	0.21007385846216664
1103	28-29	0.2866210809666825
1103	30-31	0.2637977542660579
1103	32-33	0.2874933989731048
1103	34-35	0.22136252189468308
1103	36-37	0.25575287115031387
1103	38-39	0.26044276087874607
1103	40-41	0.18253599691306022
1103	42-43	0.20958526951230994
1103	44-45	0.21138534272741794
1103	46-47	0.20668693308648756
1103	48-49	0.20578345203801973
1103	50-51	0.251502064619757
1103	52-53	0.248703370963824
1103	54-55	0.2729708693750581
1103	56-57	0.28291148115050646
1103	58-59	0.2618645769769188
1103	60-61	0.2912669254787019
1103	62-63	0.2903209991627662
1103	64-65	0.2662628494070205
1103	66-67	0.28996677142397687
1103	68-69	0.24226128754526144
1103	70-71	0.18042947044399682
1103	72-73	0.1977692470625385
1103	74-75	0.21828626203660662
1103	76-77	0.17763275434230508
1103	78-79	0.19191207867401516
1103	80-81	0.18436540228705667
1103	82-83	0.21067993377513972
1103	84-85	0.11178675666345583
1103	86-87	0.10752516083118735
1103	88-89	0.15516582964674797
1103	90-91	0.142648741588836
1103	92-93	0.19682460904162724
1103	94-95	0.1906858942543721
1103	96-97	0.19436154478896128
1103	98-99	0.19207369985669231
1103	100	0.18471322274355373
1104	1	0.18551558370698729
1104	2	0.17725930035765458
1104	3	0.22073827895207643
1104	4	0.12374076943869028
1104	5	0.11426888595953244
1104	6	0.11585950029929393
1104	7	0.07215442944517036
1104	8	0.10023573061939572
1104	9	0.1382952093410097
1104	10-11	0.12345204005874422
1104	12-13	0.11991854907648047
1104	14-15	0.16446130241823909
1104	16-17	0.2075932825862381
1104	18-19	0.15902043245506547
1104	20-21	0.21032884084175407
1104	22-23	0.23339001085189892
1104	24-25	0.23864170990903233
1104	26-27	0.23074199114675054
1104	28-29	0.2697011039158852
1104	30-31	0.241311107542721
1104	32-33	0.20999689657101328
1104	34-35	0.22089706038818946
1104	36-37	0.19589416201715437
1104	38-39	0.1728077986874368
1104	40-41	0.20561560982160643
1104	42-43	0.17133394758039344
1104	44-45	0.19085133091563478
1104	46-47	0.17956919554387696
1104	48-49	0.2345355528838411
1104	50-51	0.19994484123874656
1104	52-53	0.18775086608907543
1104	54-55	0.14189724404049286
1104	56-57	0.16814391540025042
1104	58-59	0.16970891638996477
1104	60-61	0.11970333584871895
1104	62-63	0.08862305505115131
1104	64-65	0.12704289215579934
1104	66-67	0.1207486634398407
1104	68-69	0.14180773518813794
1104	70-71	0.09118599711526798
1104	72-73	0.12761937474692076
1104	74-75	0.03976684701925848
1104	76-77	0.11642348395984925
1104	78-79	0.1409071849774719
1104	80-81	0.08926194134775756
1104	82-83	0.09770539746416063
1104	84-85	0.081144956431352
1104	86-87	0.08100451438491518
1104	88-89	0.12649369489987095
1104	90-91	0.08402158389274206
1104	92-93	0.07759691166013738
1104	94-95	0.09763508711137092
1104	96-97	0.015786055484653616
1104	98-99	0.018785897374101523
1104	100	-0.02329044442496553
1105	1	0.14734512941441125
1105	2	0.1675510856688831
1105	3	0.15120468415069155
1105	4	0.10708484600554868
1105	5	0.10914448888506456
1105	6	0.06787909528888747
1105	7	0.10799889203171631
1105	8	0.10566522185494165
1105	9	0.1137608007273414
1105	10-11	0.10443921296450043
1105	12-13	0.09601772161585131
1105	14-15	0.12804692439448928
1105	16-17	0.11286342535161964
1105	18-19	0.12156983936705501
1105	20-21	0.11736607273465438
1105	22-23	0.17141811065452828
1105	24-25	0.14235267872388135
1105	26-27	0.1186931984104973
1105	28-29	0.16355296289022903
1105	30-31	0.12896065741681184
1105	32-33	0.16135894794607708
1105	34-35	0.14684473776489426
1105	36-37	0.14610265441983472
1105	38-39	0.16985872601950547
1105	40-41	0.1759688992903392
1105	42-43	0.1375155092143885
1105	44-45	0.14964409982704296
1105	46-47	0.15350519579664734
1105	48-49	0.2264305825680566
1105	50-51	0.17771194920205602
1105	52-53	0.1362561819200181
1105	54-55	0.10418676647508818
1105	56-57	0.12708192113255734
1105	58-59	0.12337170584681445
1105	60-61	0.07836144408111778
1105	62-63	0.16686578939357588
1105	64-65	0.16333931866543594
1105	66-67	0.12621478192438218
1105	68-69	0.15082760823342056
1105	70-71	0.1904288478901961
1105	72-73	0.08216898414360685
1105	74-75	0.049225468557203556
1105	76-77	0.022731227890687933
1105	78-79	0.08512401768386724
1105	80-81	0.07250168057238682
1105	82-83	0.10121265117847145
1105	84-85	0.06979495236419098
1105	86-87	0.07183317655727706
1105	88-89	0.059213938592399984
1105	90-91	0.021857661212735024
1105	92-93	-0.01172346736144192
1105	94-95	0.040172193039950344
1105	96-97	-0.004574422722527061
1105	98-99	-0.020178375165443185
1105	100	0.09710968387103946
1106	1	0.11378310766334465
1106	2	0.10947742809819516
1106	3	0.13849846382339592
1106	4	0.06262505355441306
1106	5	0.03943427507500985
1106	6	0.024643585036841387
1106	7	0.02738750508130039
1106	8	0.03398145237202499
1106	9	0.0730409566082173
1106	10-11	0.07035537262663638
1106	12-13	0.07240213773780368
1106	14-15	0.1546474130129809
1106	16-17	0.09986007026488863
1106	18-19	0.07288567176354377
1106	20-21	0.07331965923994943
1106	22-23	0.10114458251687353
1106	24-25	0.11733944603730606
1106	26-27	0.08726373503144913
1106	28-29	0.09349140327474004
1106	30-31	0.06369748567600197
1106	32-33	0.10603112983983465
1106	34-35	0.06504835452384583
1106	36-37	0.09887036420921902
1106	38-39	0.10055989179376468
1106	40-41	0.10074010271943479
1106	42-43	0.0652447486286789
1106	44-45	0.09879303375831938
1106	46-47	0.07121659870696107
1106	48-49	0.13561347074877972
1106	50-51	0.09110498893168284
1106	52-53	0.08763455607970627
1106	54-55	0.03875691023696248
1106	56-57	0.034770524185276486
1106	58-59	0.01740349235596028
1106	60-61	0.051225316654466724
1106	62-63	0.018039718984105946
1106	64-65	0.1259862242863008
1106	66-67	0.11596293118978451
1106	68-69	0.0714967489769407
1106	70-71	-4.1618401965592966E-5
1106	72-73	0.03160865133584423
1106	74-75	0.022416098166502252
1106	76-77	0.017843552266015195
1106	78-79	-0.003187284556311454
1106	80-81	0.02800600465306502
1106	82-83	0.05797049389102682
1106	84-85	0.02441818471753976
1106	86-87	0.014873362183195127
1106	88-89	0.04466003534447793
1106	90-91	0.050232487363821576
1106	92-93	0.057436971728733965
1106	94-95	0.008368776919752463
1106	96-97	0.01772676690389119
1106	98-99	0.07257251534438325
1106	100	0.041968473829982145
1107	1	0.08837669772935186
1107	2	0.12373964554473815
1107	3	0.13382632544772832
1107	4	0.11800752340067788
1107	5	0.0485667816642561
1107	6	0.05800202334620508
1107	7	0.053472990464129566
1107	8	0.07585576854448561
1107	9	0.05639361402327836
1107	10-11	0.11699268846270172
1107	12-13	0.06711128036912584
1107	14-15	0.10230889429366385
1107	16-17	0.11020316797505814
1107	18-19	0.1065852154974749
1107	20-21	0.10253280440571899
1107	22-23	0.11353712157056606
1107	24-25	0.1125425448579378
1107	26-27	0.1364834122811871
1107	28-29	0.11735415112650571
1107	30-31	0.16125223270472588
1107	32-33	0.11171123409189221
1107	34-35	0.15960229484019095
1107	36-37	0.1867531524945818
1107	38-39	0.1847873912140443
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2216	36-37	-0.48882318230354116
2216	38-39	-0.45670079297334354
2216	40-41	-0.4287912062525905
2216	42-43	-0.5208956026093574
2216	44-45	-0.4867811448882904
2216	46-47	-0.467685085701234
2216	48-49	-0.4547403794078946
2216	50-51	-0.4472744353522202
2216	52-53	-0.4758274637498019
2216	54-55	-0.5446125746091255
2216	56-57	-0.5092048601516552
2216	58-59	-0.48651587530451224
2216	60-61	-0.4900442267072904
2216	62-63	-0.49466558722564713
2216	64-65	-0.49329427970952366
2216	66-67	-0.49011191128580833
2216	68-69	-0.4765252977553871
2216	70-71	-0.4269750164556285
2216	72-73	-0.45174313483204287
2216	74-75	-0.449883321098838
2216	76-77	-0.4131343279856168
2216	78-79	-0.4667273389995401
2216	80-81	-0.43630663154499416
2216	82-83	-0.3879307143702704
2216	84-85	-0.3714006902388789
2216	86-87	-0.3602857665194179
2216	88-89	-0.4199050409778593
2216	90-91	-0.40829998266617906
2216	92-93	-0.4142153505414683
2216	94-95	-0.4474408424815337
2216	96-97	-0.44150402447692727
2216	98-99	-0.48297265227886044
2216	100	-0.6361503037374305
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	23.0
8	100.0
9	152.0
10	304.0
11	278.0
12	199.0
13	180.0
14	358.0
15	605.0
16	1134.0
17	2111.0
18	3877.0
19	6029.0
20	8586.0
21	12734.0
22	18306.0
23	22776.0
24	23073.0
25	20595.0
26	19337.0
27	20973.0
28	25624.0
29	31899.0
30	39382.0
31	50688.0
32	66125.0
33	88645.0
34	132757.0
35	213743.0
36	403423.0
37	1009636.0
38	1319802.0
39	457207.0
40	1244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.326115743495386	19.074538835018178	13.098992192987957	23.50035322849848
2	14.511456090198108	21.135629803592018	40.10301852532695	24.249895580882924
3	18.886521397009563	27.237198614355062	30.933801045555715	22.942478943079657
4	12.22242906815629	17.364330237724285	39.24188605176784	31.171354642351577
5	12.48455448034435	39.36987029388171	35.05977350487495	13.08580172089899
6	28.758070303980197	37.71242179589315	19.383598203567075	14.145909696559569
7	25.407206576875986	34.13985856718059	22.744846198055924	17.7080886578875
8	24.589464267642537	34.255485824875905	21.557683153398195	19.597366754083367
9	26.45847415168526	16.42217893728112	20.88250470713323	36.236842203900395
10-11	23.966161115768614	25.63682796068372	28.591320883429265	21.805690040118396
12-13	26.860557659414702	23.222902592640253	28.172307938344364	21.744231809600677
14-15	24.40979343832336	24.342213106561278	26.819134092172124	24.42885936294324
16-17	21.583383413649248	27.551653525008714	28.469578863066463	22.395384198275572
18-19	21.16028491430956	27.89910055336146	29.863802364124087	21.076812168204892
20-21	22.226014860422723	27.305920555335522	28.320262474996284	22.14780210924547
22-23	22.208487026549616	26.447302514602804	27.41534644490614	23.928864013941432
24-25	22.304764855737456	26.889144045148498	28.175993683008464	22.63009741610558
26-27	23.194191266409373	25.88984496133716	28.718760190459292	22.19720358179417
28-29	22.2518164797868	25.998297059960855	29.518410306098165	22.23147615415418
30-31	22.058958024417414	27.0350343761885	29.20815323129175	21.697854368102334
32-33	22.02872823473761	27.487882470269355	28.301156940782597	22.182232354210445
34-35	22.34597985988026	26.95009799186029	28.507485138796607	22.196437009462844
36-37	23.076288759746824	25.94961293821742	28.48980622984619	22.484292072189564
38-39	23.75793501260906	25.414517262369422	28.910170809548703	21.91737691547281
40-41	22.91613410087777	25.46807691336988	29.89035329383663	21.72543569191572
42-43	22.712383145634192	26.157586316241627	28.754526306741102	22.375504231383076
44-45	22.064445323751524	27.28057016081283	28.50514200982861	22.14984250560704
46-47	22.3400608111755	27.02211651677105	27.018105917794966	23.619716754258484
48-49	22.965029582952525	26.771363227181205	27.123534986347053	23.140072203519217
50-51	23.423538797053638	26.634852472555103	26.842292346164587	23.09931638422667
52-53	22.577904896747143	26.952980877221677	27.573968592779764	22.895145633251417
54-55	22.51432181187178	26.77591279334398	27.826926568096432	22.882838826687806
56-57	22.851529410000825	27.105353247356863	27.316879358899715	22.7262379837426
58-59	21.9546566828323	27.094203174694403	27.704765540028525	23.24637460244477
60-61	22.631873746745082	26.652297405474172	27.78542913934739	22.930399708433356
62-63	23.745843968480195	26.46503898370372	26.90363454334848	22.885482504467607
64-65	22.59233409250727	26.429873369387845	27.821400708395743	23.15639182970914
66-67	22.843057877467995	25.998630592268846	28.15037496282858	23.007936567434584
68-69	22.828577572295995	26.756573762972575	27.537767440105426	22.87708122462601
70-71	22.343701018643543	26.808033251691754	27.201901924401557	23.646363805263153
72-73	22.43779454676626	26.378883069416265	27.31291134090795	23.870411042909524
74-75	22.633850776427025	27.405238269506633	27.007813186221412	22.953097767844927
76-77	23.13757628060392	26.162087154521846	26.806367057249513	23.893969507624718
78-79	22.30629527094411	26.523628787191612	27.424893368786694	23.745182573077585
80-81	22.31748245772074	27.287480481161086	27.550130525402537	22.844906535715634
82-83	22.76756325340004	26.31275473059731	27.64258572710284	23.27709628889981
84-85	22.604011467045986	26.357126391759923	27.15537172632267	23.883490414871428
86-87	22.55983651781752	26.921810218336073	27.64281701909891	22.8755362447475
88-89	23.208267369899037	26.02250455189864	27.534623228482758	23.234604849719563
90-91	22.56921391187447	25.824351152763498	27.879472401268895	23.726962534093136
92-93	22.568589209389028	26.792402618253057	27.264590238898723	23.374417933459192
94-95	22.20997527552194	26.72933599489943	27.568124126900077	23.492564602678552
96-97	22.43462311893685	25.965739959281763	27.04196826876284	24.557668653018542
98-99	22.05756107397795	26.649118285029683	27.39997628619588	23.893344354796493
100	22.264521807011608	27.2660236019189	26.69821518796925	23.771239403100246
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	417.0
1	264.5
2	312.0
3	519.5
4	347.5
5	236.0
6	485.5
7	1169.0
8	2227.5
9	2770.0
10	2092.0
11	1370.0
12	1369.5
13	1572.0
14	1847.0
15	2146.0
16	2533.5
17	3029.0
18	3628.0
19	4302.0
20	5056.5
21	5896.0
22	7142.5
23	8825.0
24	10834.5
25	13377.5
26	16916.5
27	21492.5
28	26370.0
29	32620.5
30	40228.0
31	47311.0
32	55471.0
33	63146.0
34	69601.0
35	76290.0
36	83801.5
37	95161.0
38	101737.0
39	101787.5
40	102703.0
41	104168.5
42	105643.5
43	115954.0
44	147400.5
45	175309.5
46	173177.5
47	177812.5
48	258064.0
49	256184.5
50	184164.5
51	168442.0
52	148590.5
53	140561.0
54	134196.5
55	120244.5
56	108081.5
57	93966.0
58	78323.5
59	69876.5
60	58456.0
61	43866.0
62	32562.0
63	25156.0
64	18528.5
65	12143.5
66	8503.0
67	6254.0
68	4552.5
69	3301.0
70	2526.0
71	2005.0
72	1579.0
73	1280.5
74	944.0
75	690.5
76	477.5
77	299.0
78	190.0
79	112.0
80	58.5
81	28.0
82	11.5
83	5.5
84	3.0
85	2.5
86	2.5
87	2.0
88	1.5
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006147072456742477
2	0.08983221740646018
3	3.4983339184713284E-4
4	0.0
5	0.03883150649503174
6	0.004922655585277512
7	1.249404970882617E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.4988099417652342E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.12256038061873E-4
24-25	0.0
26-27	0.0
28-29	6.247024854413085E-5
30-31	1.7491669592356642E-4
32-33	0.0014493097662238359
34-35	0.0010495001755413983
36-37	6.122084357324824E-4
38-39	5.247500877706992E-4
40-41	0.0012993811697179219
42-43	5.497381871883515E-4
44-45	4.1230364039126366E-4
46-47	2.248928947588711E-4
48-49	5.622322368971777E-4
50-51	5.622322368971777E-4
52-53	0.0013618514182620527
54-55	0.0013618514182620527
56-57	8.745834796178321E-4
58-59	0.0025612801903093655
60-61	0.0018866015060327518
62-63	0.00101201802641492
64-65	0.004672774591100988
66-67	0.004372917398089159
68-69	0.0036107803658507634
70-71	0.001586744313020924
72-73	0.005147548480036382
74-75	0.0036107803658507634
76-77	0.0037607089623566776
78-79	6.996667836942657E-4
80-81	4.9976198835304684E-5
82-83	1.3743454679708789E-4
84-85	6.247024854413085E-5
86-87	2.4988099417652342E-5
88-89	8.745834796178321E-5
90-91	0.0
92-93	0.0
94-95	1.2494049708826171E-5
96-97	3.123512427206543E-4
98-99	4.1230364039126366E-4
100	6.746786842766133E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4001905.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.63335584039196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.36966724677076	35.81788713482098
2	15.269219274381573	15.768020643982306
3	6.143675965891606	9.516558219448351
4	3.182088218580413	6.572075732219256
5	1.7993629209882651	4.645357299269707
6	1.1229408773325575	3.478872354422032
7	0.7397485434825976	2.673698984463403
8	0.5209368369135525	2.1518173656580553
9	0.37718848579778297	1.7527956575486003
>10	1.437525442825588	11.633180970699767
>50	0.026912729188862648	0.9215149783682713
>100	0.008489573455309705	0.7899665113955416
>500	9.97281951673586E-4	0.3734101723485282
>1k	6.648546344489845E-4	0.754801761849006
>5k	4.155341465306154E-4	1.405072705513579
>10k+	1.6621365861224614E-4	1.7449695079925998
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	29978	0.7490932443423819	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10687	0.2670478184764506	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9520	0.2378867064560503	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	7045	0.17604116039736076	No Hit
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA	5718	0.1428819524701361	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	5231	0.13071274805373942	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5230	0.13068775995432175	No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT	4519	0.11292122126837092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.4988099417652342E-5	0.0
10-11	0.0	0.0	0.0	3.748214912647851E-5	0.0
12-13	0.0	0.0	0.0	6.247024854413085E-5	0.0
14-15	0.0	0.0	0.0	1.1244644737943554E-4	0.0
16-17	0.0	0.0	0.0	1.749166959235664E-4	0.0
18-19	0.0	0.0	0.0	1.9990479534121874E-4	0.0
20-21	0.0	0.0	0.0	2.9985719301182814E-4	0.0
22-23	0.0	0.0	0.0	6.871727339854395E-4	0.0
24-25	0.0	0.0	0.0	0.0011244644737943555	0.0
26-27	0.0	0.0	0.0	0.0022989051464240157	0.0
28-29	0.0	0.0	0.0	0.0060846022081983454	0.0
30-31	0.0	0.0	0.0	0.016667062311574114	0.0
32-33	0.0	0.0	0.0	0.03929378633425831	0.0
34-35	0.0	0.0	0.0	0.07951213234696976	0.0
36-37	0.0	0.0	0.0	0.14261957742625075	0.0
38-39	0.0	0.0	0.0	0.22380591243420322	0.0
40-41	0.0	0.0	0.0	0.3051546700883704	0.0
42-43	0.0	0.0	0.0	0.3792318908120008	0.0
44-45	0.0	0.0	0.0	0.4686143224289432	0.0
46-47	0.0	0.0	0.0	0.5808358769136199	0.0
48-49	0.0	0.0	0.0	0.7013534804049572	0.0
50-51	0.0	0.0	0.0	0.8201094228873499	0.0
52-53	0.0	0.0	0.0	0.9351796207056389	0.0
54-55	0.0	0.0	0.0	1.0530609797084138	0.0
56-57	0.0	0.0	0.0	1.1869846985373216	0.0
58-59	0.0	0.0	0.0	1.3490575113602148	0.0
60-61	0.0	0.0	0.0	1.5258857968892316	0.0
62-63	0.0	0.0	0.0	1.7037136063949543	0.0
64-65	0.0	0.0	0.0	1.8881132860475198	0.0
66-67	0.0	0.0	0.0	2.079584597835281	0.0
68-69	0.0	0.0	0.0	2.274504267342678	0.0
70-71	0.0	0.0	0.0	2.4855912371732964	0.0
72-73	0.0	0.0	0.0	2.701113594650548	0.0
74-75	0.0	0.0	0.0	2.912500421674178	0.0
76-77	0.0	0.0	0.0	3.1387051916524755	0.0
78-79	0.0	0.0	0.0	3.3815770239423477	0.0
80-81	0.0	0.0	0.0	3.641290835239717	0.0
82-83	0.0	0.0	0.0	3.9035409386279785	0.0
84-85	0.0	0.0	0.0	4.195776761317423	0.0
86-87	0.0	0.0	0.0	4.50053162181511	0.0
88	0.0	0.0	0.0	4.716803622274892	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACT	11435	0.0	22.213907	12-13
CTAACGC	995	0.0	21.737068	3
TCTAACG	1065	0.0	21.651201	2
GTACCCG	355	0.0	21.211199	1
CGCCTAC	1115	0.0	20.653164	7
AGTACTT	12465	0.0	20.585707	12-13
CATGGGG	8285	0.0	20.543613	4
CTGTGCG	2325	0.0	20.41568	9
TACCTGG	3170	0.0	20.189018	2
TAACGCC	1150	0.0	20.033602	4
GTACTTT	13110	0.0	19.30405	14-15
CTAGTAC	2655	0.0	19.126059	3
TAGTACT	2640	0.0	19.05639	4
GGCGTCG	1555	0.0	18.73815	8
CTATTGA	2780	0.0	18.595732	9
TAGGCAT	3015	0.0	18.55754	5
ATTTAGG	3165	0.0	18.438328	1
CTAGGCA	3175	0.0	18.362795	4
GCTATTG	2745	0.0	18.319214	8
GGGCTAT	2875	0.0	18.308884	6
>>END_MODULE