FastQCFastQC Report
Mon 16 Jan 2017
ERR1146572_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146572_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5847969
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3118035.331816909426162No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT836511.430428239274182No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC695061.1885493921051906No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC602471.0302209194337384No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA519820.8888898008864274No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA493720.8442589213451713No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC401430.6864434472891358No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT95050.16253506131786952No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC78990.13507253543922684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20200.028.1647951
GAGTACG49000.022.2620141
CTAACGC9000.021.4078223
GTACGCA49350.021.3300553
TACGCAG49850.021.1159344
CATGGGG92700.020.88554
GCATCGT23200.020.8622828
TAACGCC11000.020.0785624
CGCAGTG51850.019.8474276
AGTACGC53800.019.8278062
CGACGAG12500.019.35986524-25
TAGGCAT30100.019.2028665
TACTAGG30200.018.8282322
CAATGCG13000.018.79638718-19
CTAGGCA34500.018.6607424
CGCCTAC9850.018.6048457
CTAGTAC29400.018.5413683
TAGTACT27850.018.3919894
GTATATA14700.018.2318231
TCTAACG10850.018.1907542
Produced by FastQC (version 0.11.5)