##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146572_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5847969
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75755941934713	31.0	31.0	34.0	30.0	34.0
2	32.00200445658997	33.0	31.0	34.0	30.0	34.0
3	32.16243690758279	34.0	31.0	34.0	30.0	34.0
4	35.59666048161336	37.0	35.0	37.0	33.0	37.0
5	35.611540690451676	37.0	35.0	37.0	35.0	37.0
6	35.809414174391144	37.0	36.0	37.0	35.0	37.0
7	35.82370973580743	37.0	36.0	37.0	35.0	37.0
8	35.80580591312984	37.0	37.0	37.0	35.0	37.0
9	37.53641084622713	39.0	38.0	39.0	35.0	39.0
10-11	37.51389234450456	39.0	38.0	39.0	35.0	39.0
12-13	37.508703363509625	39.0	38.0	39.0	35.0	39.0
14-15	38.85277504036017	40.0	39.0	41.0	36.0	41.0
16-17	38.932557525527244	40.5	39.0	41.0	36.0	41.0
18-19	38.983456478650965	41.0	39.0	41.0	36.0	41.0
20-21	39.006647521558335	41.0	39.0	41.0	36.0	41.0
22-23	38.95792727355429	41.0	39.0	41.0	36.0	41.0
24-25	38.91650186586146	40.5	39.0	41.0	35.5	41.0
26-27	38.78574971926151	40.5	38.5	41.0	35.0	41.0
28-29	38.75777137327506	40.0	38.5	41.0	35.0	41.0
30-31	38.801449015889105	40.5	39.0	41.0	35.0	41.0
32-33	38.71652047061125	40.0	38.0	41.0	35.0	41.0
34-35	38.505406748907184	40.0	38.0	41.0	34.0	41.0
36-37	38.35966358576798	40.0	38.0	41.0	33.5	41.0
38-39	38.248379138124704	40.0	38.0	41.0	33.0	41.0
40-41	38.31698030547015	40.0	38.0	41.0	33.5	41.0
42-43	38.154376331338284	40.0	38.0	41.0	33.0	41.0
44-45	37.94872365089486	40.0	38.0	41.0	33.0	41.0
46-47	37.72465611565315	40.0	37.0	41.0	33.0	41.0
48-49	37.49498492553569	40.0	37.0	41.0	32.0	41.0
50-51	37.21992609741946	39.0	36.0	41.0	31.0	41.0
52-53	37.23343848094954	39.0	36.0	41.0	31.5	41.0
54-55	37.01149689746987	39.0	36.0	41.0	31.0	41.0
56-57	36.59057187204652	39.0	35.0	40.5	30.0	41.0
58-59	36.284784768865904	38.5	35.0	40.0	29.0	41.0
60-61	35.830432240663384	38.0	35.0	40.0	28.0	41.0
62-63	35.729974799798015	37.5	34.5	40.0	28.5	41.0
64-65	35.48842709323527	37.0	34.5	39.5	29.0	41.0
66-67	35.032223922527635	36.5	34.0	39.0	28.0	41.0
68-69	34.5104596142695	36.0	34.0	39.0	27.5	40.0
70-71	33.98634081336614	35.0	33.0	37.5	26.5	40.0
72-73	33.58196871426644	35.0	32.5	37.0	26.0	39.0
74-75	33.38933756317792	35.0	33.0	37.0	26.0	39.0
76-77	33.1489653929424	35.0	33.0	36.0	26.0	38.0
78-79	32.7884661324299	35.0	32.0	36.0	26.0	37.0
80-81	32.335169953876296	35.0	32.0	35.0	25.5	37.0
82-83	32.07638318534178	35.0	31.5	35.0	25.0	36.0
84-85	31.970003773959814	35.0	31.0	35.0	25.0	36.0
86-87	32.089003635279184	35.0	32.0	35.0	25.0	36.0
88-89	31.96063137475592	35.0	32.0	35.0	25.0	35.5
90-91	31.70012836935353	35.0	31.0	35.0	24.5	35.0
92-93	31.52264640937734	34.0	31.0	35.0	24.0	35.0
94-95	31.45690093432438	34.0	31.0	35.0	23.5	35.0
96-97	31.349914389081064	34.0	31.0	35.0	23.5	35.0
98-99	31.524561689708	34.0	31.0	35.0	24.0	35.0
100	30.333378648211028	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17824354225584216
1101	2	0.23856980918515802
1101	3	0.2875555494304649
1101	4	0.2551802238085159
1101	5	0.2168330057131982
1101	6	0.19839379178873173
1101	7	0.19798284370691022
1101	8	0.2311465976943694
1101	9	0.3278575881923089
1101	10-11	0.3247734800927802
1101	12-13	0.3288865212131995
1101	14-15	0.4630010296746718
1101	16-17	0.4400406806371464
1101	18-19	0.3863773535880881
1101	20-21	0.40682463692895965
1101	22-23	0.40516419667322623
1101	24-25	0.41802025457568703
1101	26-27	0.42135786352503857
1101	28-29	0.4733977828241933
1101	30-31	0.4955604328607919
1101	32-33	0.45373292521414044
1101	34-35	0.5092120815639234
1101	36-37	0.5397292299657934
1101	38-39	0.5055646137085077
1101	40-41	0.47433902795395966
1101	42-43	0.434623292316779
1101	44-45	0.4357843547652962
1101	46-47	0.4397873444487317
1101	48-49	0.4543090465687243
1101	50-51	0.5115523393959052
1101	52-53	0.48159253867792984
1101	54-55	0.49541204540983586
1101	56-57	0.5074562065401267
1101	58-59	0.5018709618052881
1101	60-61	0.5437595768683607
1101	62-63	0.5602701460445445
1101	64-65	0.5065084417405714
1101	66-67	0.5153230300742564
1101	68-69	0.4876316871876156
1101	70-71	0.5389358467115031
1101	72-73	0.49549492067150425
1101	74-75	0.47507806725756296
1101	76-77	0.4572902920244246
1101	78-79	0.42693024925674905
1101	80-81	0.5374402817057558
1101	82-83	0.5130812711598978
1101	84-85	0.4642311308004494
1101	86-87	0.4516899927331153
1101	88-89	0.42186813761404807
1101	90-91	0.47637071043138235
1101	92-93	0.521182340189636
1101	94-95	0.43860167476220013
1101	96-97	0.33308460426095365
1101	98-99	0.40081143449099343
1101	100	0.4662834241970799
1102	1	0.12280998812891397
1102	2	0.15012901939733325
1102	3	0.2059346146897596
1102	4	0.1800126677763032
1102	5	0.14294961472588597
1102	6	0.1604083785818915
1102	7	0.13400035976895452
1102	8	0.1674613798992155
1102	9	0.2497676443502499
1102	10-11	0.2431839096262891
1102	12-13	0.26813562871576835
1102	14-15	0.3198704321049064
1102	16-17	0.35126476062902867
1102	18-19	0.28869506905611075
1102	20-21	0.34958276672131205
1102	22-23	0.3088858424161529
1102	24-25	0.3247099773881317
1102	26-27	0.32615025200121295
1102	28-29	0.345801951122624
1102	30-31	0.34410705506009265
1102	32-33	0.34738511384133375
1102	34-35	0.3609742726439791
1102	36-37	0.4305435463816636
1102	38-39	0.3707677715961708
1102	40-41	0.3343586290367
1102	42-43	0.3185301152764808
1102	44-45	0.3080247116807513
1102	46-47	0.33049044705175845
1102	48-49	0.42941493617119875
1102	50-51	0.36705842624380125
1102	52-53	0.3397820820195534
1102	54-55	0.42319474645573507
1102	56-57	0.4005416504485524
1102	58-59	0.4746249756607668
1102	60-61	0.46020500511915685
1102	62-63	0.4575565144864342
1102	64-65	0.36769699068489814
1102	66-67	0.3655243061054634
1102	68-69	0.4481500301589918
1102	70-71	0.3598335131187227
1102	72-73	0.3748440652302065
1102	74-75	0.33246527524526925
1102	76-77	0.33918397339861883
1102	78-79	0.3378064717599045
1102	80-81	0.3498758396966224
1102	82-83	0.39311231407512537
1102	84-85	0.37369610621104954
1102	86-87	0.37686223298109667
1102	88-89	0.35168221782198117
1102	90-91	0.44715190689991857
1102	92-93	0.42010762131974033
1102	94-95	0.4122315643436174
1102	96-97	0.32667238492127737
1102	98-99	0.3671014030613584
1102	100	0.313295684314852
1103	1	0.0735936689688117
1103	2	0.09574533217006831
1103	3	0.11280762982798365
1103	4	0.009617645233149119
1103	5	-0.005381414478193847
1103	6	0.0266968357787718
1103	7	0.00454829735149076
1103	8	0.08897259190346318
1103	9	0.15046039931944932
1103	10-11	0.13568852420227273
1103	12-13	0.11152078304780844
1103	14-15	0.17436272067813263
1103	16-17	0.1208704918810497
1103	18-19	0.08329997097214203
1103	20-21	0.15981502004681403
1103	22-23	0.15981615308460562
1103	24-25	0.18493998061891403
1103	26-27	0.19037434810160647
1103	28-29	0.21633252238268597
1103	30-31	0.199697830736298
1103	32-33	0.21169886425352047
1103	34-35	0.2169507097165706
1103	36-37	0.24035338945193985
1103	38-39	0.2116279002779322
1103	40-41	0.1800998234106288
1103	42-43	0.19439459387254487
1103	44-45	0.22456808014621288
1103	46-47	0.20010616797161873
1103	48-49	0.22908365340806824
1103	50-51	0.25716568183418786
1103	52-53	0.19088511501281857
1103	54-55	0.267754305065111
1103	56-57	0.2591117182100078
1103	58-59	0.29044797509133247
1103	60-61	0.17921573014053394
1103	62-63	0.2213949330607221
1103	64-65	0.23034889694525873
1103	66-67	0.2921063767377916
1103	68-69	0.23779165862109863
1103	70-71	0.20143714084133535
1103	72-73	0.23650434372473228
1103	74-75	0.23491361487161555
1103	76-77	0.25741101489181517
1103	78-79	0.24717292519716239
1103	80-81	0.2946771719247394
1103	82-83	0.18828581519616705
1103	84-85	0.19862422012035807
1103	86-87	0.16681142815420458
1103	88-89	0.1717017379985606
1103	90-91	0.28843240274015614
1103	92-93	0.2652713720303197
1103	94-95	0.16973600660051247
1103	96-97	0.17339499157724703
1103	98-99	0.17281706840361366
1103	100	0.27018558994905106
1104	1	0.050800012946222495
1104	2	0.10211822629708678
1104	3	0.10360698192143047
1104	4	0.08606005727865096
1104	5	0.06453998593085686
1104	6	0.10207215643115575
1104	7	0.04873463901187591
1104	8	0.13160072917008137
1104	9	0.17673225599906317
1104	10-11	0.16011270201948946
1104	12-13	0.16133108303287713
1104	14-15	0.24060656102302858
1104	16-17	0.1904318929907447
1104	18-19	0.1590602332569162
1104	20-21	0.1498557820673554
1104	22-23	0.20276595425784905
1104	24-25	0.19432982149911737
1104	26-27	0.203611454221452
1104	28-29	0.17753403195209216
1104	30-31	0.20744690656395903
1104	32-33	0.1867134583352268
1104	34-35	0.24597021789883655
1104	36-37	0.25965985635868805
1104	38-39	0.2538006991221664
1104	40-41	0.22959242875153052
1104	42-43	0.2280927613229764
1104	44-45	0.23106945033560322
1104	46-47	0.20401097471258822
1104	48-49	0.2397535430133928
1104	50-51	0.26350971558203184
1104	52-53	0.18828220074448154
1104	54-55	0.2001309795688968
1104	56-57	0.2007966204365772
1104	58-59	0.24136422939003666
1104	60-61	0.2785230144592674
1104	62-63	0.2543755859024728
1104	64-65	0.26460230019088016
1104	66-67	0.2669572323417597
1104	68-69	0.28397156874469687
1104	70-71	0.30923027292128324
1104	72-73	0.2653291275740628
1104	74-75	0.2193740804543154
1104	76-77	0.18022023042317414
1104	78-79	0.24466156004643125
1104	80-81	0.29514398279599163
1104	82-83	0.2554627651206687
1104	84-85	0.20161468805184057
1104	86-87	0.20929623663447217
1104	88-89	0.21360816092075652
1104	90-91	0.25250450851530815
1104	92-93	0.27269371353869687
1104	94-95	0.1364907191905722
1104	96-97	0.17830729955841562
1104	98-99	0.11242456110094068
1104	100	0.20226946092538256
1105	1	0.05228112163102239
1105	2	0.13236532205201712
1105	3	0.14295804384046562
1105	4	0.12335140863732619
1105	5	0.07375658125451423
1105	6	0.07780973407712821
1105	7	0.0435091393216851
1105	8	0.12601812200812645
1105	9	0.18945613167991837
1105	10-11	0.1826880574460148
1105	12-13	0.13585981921294632
1105	14-15	0.23261986106604837
1105	16-17	0.16828056264528612
1105	18-19	0.15441508125831405
1105	20-21	0.1607310708037062
1105	22-23	0.16668720027206518
1105	24-25	0.1769705319495003
1105	26-27	0.20910409579904154
1105	28-29	0.18608897901334842
1105	30-31	0.21312439642517234
1105	32-33	0.17851169466381123
1105	34-35	0.1457160911500921
1105	36-37	0.17716739772340162
1105	38-39	0.20085720202584412
1105	40-41	0.1975472706089505
1105	42-43	0.20238320162194157
1105	44-45	0.17755811455985793
1105	46-47	0.1956232495996204
1105	48-49	0.17584671359567494
1105	50-51	0.18402408071699483
1105	52-53	0.15584056617760922
1105	54-55	0.23506989987232885
1105	56-57	0.21091654742750165
1105	58-59	0.24091074966955262
1105	60-61	0.22131743584345287
1105	62-63	0.24116021495644446
1105	64-65	0.29317696824637807
1105	66-67	0.287449188566427
1105	68-69	0.30108911082279377
1105	70-71	0.23969733509055402
1105	72-73	0.22736272200779695
1105	74-75	0.2227363139302767
1105	76-77	0.23207843056756872
1105	78-79	0.2528902877664905
1105	80-81	0.20501159292086868
1105	82-83	0.1844460838644224
1105	84-85	0.2162875671328166
1105	86-87	0.21439077609669255
1105	88-89	0.22063357292293517
1105	90-91	0.31115600721189196
1105	92-93	0.27540194670783436
1105	94-95	0.27598174910767526
1105	96-97	0.149077101604977
1105	98-99	0.07939162344640138
1105	100	0.22288160617356212
1106	1	0.03793135430480987
1106	2	0.05146406401571113
1106	3	0.10331185239935792
1106	4	0.09815989795662006
1106	5	0.0862487672311616
1106	6	0.0838661612543774
1106	7	0.05007304802810353
1106	8	0.12048460659298854
1106	9	0.12412437354657158
1106	10-11	0.13824941182620876
1106	12-13	0.10940836015153366
1106	14-15	0.1521693298537059
1106	16-17	0.12847028829407492
1106	18-19	0.10046137338473926
1106	20-21	0.11623343036120559
1106	22-23	0.133472089961316
1106	24-25	0.1306397464220126
1106	26-27	0.16290142980410138
1106	28-29	0.1537664681912645
1106	30-31	0.17638141242375127
1106	32-33	0.13607271459066794
1106	34-35	0.156296735523604
1106	36-37	0.206944649098034
1106	38-39	0.2072214896872424
1106	40-41	0.2149567194791473
1106	42-43	0.18840024404085653
1106	44-45	0.14499167392838785
1106	46-47	0.16564768328235147
1106	48-49	0.13864316144062627
1106	50-51	0.21303482463935097
1106	52-53	0.2096882899167838
1106	54-55	0.2647910723738178
1106	56-57	0.18797469181047433
1106	58-59	0.24070354259958293
1106	60-61	0.2807134066178065
1106	62-63	0.2822675021406198
1106	64-65	0.22732220722750895
1106	66-67	0.2184016070891488
1106	68-69	0.19496141146034773
1106	70-71	0.15939048273521195
1106	72-73	0.16717392429919897
1106	74-75	0.12486815552053088
1106	76-77	0.1948794573645216
1106	78-79	0.1909060169146386
1106	80-81	0.1812602423768226
1106	82-83	0.18993758378800862
1106	84-85	0.23707410320968592
1106	86-87	0.1335038548122469
1106	88-89	0.1539902829579205
1106	90-91	0.17356188639793402
1106	92-93	0.0784424574023781
1106	94-95	0.06026772289988358
1106	96-97	0.06366430012180402
1106	98-99	0.1274575811920755
1106	100	0.1543419390617018
1107	1	0.06997753028906928
1107	2	0.07511152969507862
1107	3	0.1475033233379861
1107	4	0.09039099658988192
1107	5	0.09703752171159152
1107	6	0.1253857260002036
1107	7	0.07565634886595518
1107	8	0.10858819626249527
1107	9	0.1549116111536506
1107	10-11	0.15965747250159978
1107	12-13	0.11771488989517565
1107	14-15	0.1821938152429965
1107	16-17	0.1322201330935826
1107	18-19	0.16699335127828618
1107	20-21	0.12199546722545307
1107	22-23	0.14021066103191515
1107	24-25	0.159499404062025
1107	26-27	0.17164385823216577
1107	28-29	0.1619441171925473
1107	30-31	0.16646792509490638
1107	32-33	0.16903844082862918
1107	34-35	0.13440823282795833
1107	36-37	0.14366279500344348
1107	38-39	0.15711122722072446
1107	40-41	0.17349902529665684
1107	42-43	0.10649453353604343
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2216	80-81	-1.4308373543536916
2216	82-83	-1.3411909572369396
2216	84-85	-1.299789828265613
2216	86-87	-1.2525014683308981
2216	88-89	-1.336542956523175
2216	90-91	-1.3842593270192118
2216	92-93	-1.4781716120251893
2216	94-95	-1.426424763759659
2216	96-97	-1.2755574662726694
2216	98-99	-1.1939680195718196
2216	100	-1.3261487789540034
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	163.0
1	81.0
2	75.0
3	74.0
4	71.0
5	83.0
6	141.0
7	596.0
8	1804.0
9	2474.0
10	2705.0
11	3529.0
12	4897.0
13	6180.0
14	7590.0
15	8819.0
16	9429.0
17	10911.0
18	12089.0
19	13303.0
20	15470.0
21	18221.0
22	21367.0
23	25590.0
24	30862.0
25	37908.0
26	45213.0
27	51262.0
28	61169.0
29	76462.0
30	95103.0
31	119164.0
32	158863.0
33	221839.0
34	336376.0
35	538926.0
36	917474.0
37	1491004.0
38	1256652.0
39	243912.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.719908507696054	17.184801381692573	12.682391735304716	21.41289837530666
2	14.029869328315495	24.753125447313963	33.715257867466974	27.501747356903568
3	28.733408342427285	22.744395652619307	28.19757046468185	20.324625540271555
4	11.741733375813759	14.924823840480187	42.661993819816296	30.67144896388976
5	12.006071204107895	40.3894463660423	34.44890141134118	13.155581018508622
6	28.812213476224535	36.83074048877027	16.263160469048163	18.09388556595703
7	25.958502307279925	32.64755347955939	22.398698996801357	18.99524521635933
8	24.136088265260927	25.699897018389965	20.646359235836094	29.51765548051301
9	21.576814576259128	22.001472073023578	25.082992220997014	31.338721129720277
10-11	26.700238492554675	22.106264114856756	26.8412032228132	24.35229416977537
12-13	32.17160425750335	21.58500603366626	24.09987301639962	22.143516692430772
14-15	24.711051399489705	23.927937779137554	29.689782412193118	21.671228409179623
16-17	21.453801844155322	26.797140776850537	28.4189320282502	23.330125350743945
18-19	20.44643632809053	32.87126755534152	25.158956601684	21.523339514883947
20-21	24.461547288354442	25.41178888889744	22.49667831852418	27.629985504223935
22-23	21.843376877994437	26.356009988324725	24.8234408419195	26.97717229176134
24-25	27.558602692746227	25.691370908200124	23.060793921078158	23.689232477975494
26-27	25.530384006562613	21.83777018330452	30.160387716585063	22.471458093547803
28-29	21.230468516197217	31.736854886013262	25.14390494578127	21.888771652008256
30-31	23.358247682508917	24.957573266972595	24.251665281871098	27.432513768647393
32-33	24.326604400976407	26.06327657354518	25.877269602708846	23.73284942276957
34-35	27.651855474303133	24.251025498443422	25.359951642460675	22.737167384792773
36-37	25.06566484117767	22.535455166060324	29.815835721553878	22.58304427120813
38-39	23.2349050222383	26.852141179207084	27.835175764230417	22.077778034324204
40-41	22.941477634757522	28.41468544499124	25.51677434874456	23.127062571506684
42-43	21.723285766123194	28.306932809060083	21.85024910761772	28.119532317199
44-45	24.478802300652273	23.276238372643952	23.904408944943693	28.34055038176008
46-47	27.831004075801562	27.11294937645094	24.779335747660195	20.276710800087304
48-49	25.879269963056835	25.61642997925555	25.72438150455621	22.779918553131402
50-51	18.708420064661702	26.744965335122327	31.756212967953612	22.790401632262363
52-53	23.62545089056017	29.729604215245164	22.730374971263423	23.91456992293124
54-55	21.105469012940922	24.66499442209758	26.14287555645382	28.08666100850768
56-57	25.876550998642678	22.245554624293256	25.50828090242938	26.36961347463469
58-59	30.26147068966461	23.376277144014495	23.508601648757686	22.85365051756321
60-61	24.649627643974114	25.136300463759	30.12485937593149	20.0892125163354
62-63	21.747451150508144	26.06324865477982	28.218115455017724	23.971184739694316
64-65	20.532442547815133	31.88063517391127	24.69777887217706	22.889143406096537
66-67	24.32141094896024	24.993583026654324	22.4426426075569	28.242363416828542
68-69	21.699034785510126	25.91457252605785	25.092369248449565	27.29402343998246
70-71	27.157779798821817	25.236705493692085	23.268032208557603	24.33748249892849
72-73	25.332885386433745	21.7351607468511	30.10866019458663	22.82329367212853
74-75	21.64891217569821	31.040571177253355	25.014784242435233	22.295732404613197
76-77	23.203483338639163	24.6749145664447	24.362979665128858	27.758622429787277
78-79	24.400110882846935	25.518390566781214	26.009588577937752	24.0719099724341
80-81	27.41092145715799	23.85209928373469	25.38697307061747	23.350006188489854
82-83	24.749959842696274	22.419630224362578	29.831368405242703	22.999041527698445
84-85	23.12751738354018	26.594885661084277	27.55922429028312	22.71837266509242
86-87	22.892219985576602	28.074414613886695	25.5054689862704	23.5278964142663
88-89	21.889790851411664	27.702837867829587	22.248160237350657	28.159211043408096
90-91	24.495813929938407	22.967646905879793	24.030782462569636	28.50575670161216
92-93	27.572892975124052	26.564223410049458	24.94470547905839	20.918178135768105
94-95	25.231470908778952	25.29447376195459	25.972673902329667	23.501381426936792
96-97	19.374068121021512	26.105132669460744	31.281036439723458	23.23976276979428
98-99	23.89108271734895	28.959821408717257	23.22662523575151	23.922470638182286
100	23.03692960671627	20.442878868296894	24.10270769450762	32.41748383047921
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	731.0
1	508.5
2	292.5
3	315.5
4	311.5
5	259.0
6	217.5
7	212.5
8	238.5
9	270.5
10	247.0
11	215.5
12	217.5
13	215.5
14	238.5
15	272.0
16	307.5
17	377.0
18	433.5
19	470.5
20	606.5
21	816.0
22	1053.0
23	1435.5
24	1915.5
25	2532.5
26	3430.0
27	4662.5
28	6214.0
29	8519.0
30	12205.0
31	16860.5
32	23471.5
33	31797.5
34	40838.5
35	50379.0
36	60782.0
37	73247.0
38	83544.0
39	92656.0
40	98900.0
41	102782.0
42	107549.0
43	116910.0
44	130813.5
45	147737.0
46	182053.0
47	402904.0
48	882213.5
49	903648.0
50	494146.0
51	286480.0
52	205553.0
53	178179.0
54	163997.0
55	146035.5
56	132708.5
57	118385.0
58	102878.0
59	92054.0
60	76499.5
61	59291.0
62	46201.0
63	36252.5
64	27383.5
65	19071.0
66	14115.0
67	10733.5
68	8192.0
69	6638.5
70	5457.0
71	4266.0
72	3415.0
73	2948.5
74	2265.5
75	1702.0
76	1323.0
77	959.5
78	652.0
79	465.0
80	371.0
81	250.0
82	171.0
83	111.5
84	74.0
85	56.5
86	43.5
87	33.5
88	22.5
89	23.5
90	18.0
91	10.0
92	6.5
93	5.5
94	8.0
95	8.5
96	6.0
97	5.0
98	7.0
99	8.0
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06058513648071664
2	0.009678573877529104
3	0.011593768708418255
4	0.009746973692918003
5	0.01186736796997385
6	0.01236326663154336
7	0.002975391969417075
8	0.005523285092653535
9	0.007404280015848237
10-11	0.0069596812158203986
12-13	0.00621583322346613
14-15	0.010354022054494474
16-17	0.009858123392924963
18-19	0.007883078723570526
20-21	0.006899831377355112
22-23	0.002419643469382276
24-25	0.0037876397771602413
26-27	0.005053036361854859
28-29	0.006138883431153618
30-31	0.009157025285188755
32-33	0.010388221962188924
34-35	0.011807518131508564
36-37	0.01782670188573161
38-39	0.018365350431919183
40-41	0.017963501516509408
42-43	0.02393138540919078
44-45	0.02782162490943437
46-47	0.030044618909573564
48-49	0.031233065701955668
50-51	0.031634914617365444
52-53	0.03011301872496246
54-55	0.036482751533053616
56-57	0.03863734571780391
58-59	0.03613220247918551
60-61	0.03680765065615088
62-63	0.03248991230972668
64-65	0.034926655732956176
66-67	0.03667085102537308
68-69	0.03887674507166505
70-71	0.03856894590241501
72-73	0.03484970594064367
74-75	0.03792769763314409
76-77	0.0375856985561996
78-79	0.03692735033308145
80-81	0.04171533741030433
82-83	0.03775669809467184
84-85	0.03408875799444218
86-87	0.03271221170974059
88-89	0.030207068471122193
90-91	0.03271221170974059
92-93	0.034217007648296355
94-95	0.03765409837158849
96-97	0.032789161502053105
98-99	0.030284018263434707
100	0.027872924770976044
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5847969.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.825018513566256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.47336198105873	35.38513240465582
2	14.26528330409626	13.930054428475353
3	5.612731104800012	8.221251003105895
4	2.7384713491749353	5.348236572893479
5	1.5536041612736806	3.792737596847052
6	0.9367596605422024	2.7442384661241044
7	0.6054406984260925	2.0692457316664314
8	0.42849561251655854	1.6737044971282307
9	0.2990204116156691	1.3139709419762324
>10	1.070529250612597	7.864852651687078
>50	0.012871299453291712	0.4143121752442759
>100	0.0023837494387359682	0.19233109763232126
>500	2.992620014288108E-4	0.10693162762232915
>1k	2.9926199593366864E-4	0.2832920991631704
>5k	9.97539986445561E-5	0.4238301120807887
>10k+	3.491389952559464E-4	16.235878593697436
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	311803	5.331816909426162	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	83651	1.430428239274182	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	69506	1.1885493921051906	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	60247	1.0302209194337384	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	51982	0.8888898008864274	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	49372	0.8442589213451713	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	40143	0.6864434472891358	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	9505	0.16253506131786952	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	7899	0.13507253543922684	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7099953847224565E-4
2	0.0	0.0	0.0	3.4199907694449134E-5	1.7099953847224565E-4
3	0.0	0.0	0.0	3.4199907694449134E-5	1.7099953847224565E-4
4	0.0	0.0	0.0	1.7099953847224565E-4	1.7099953847224565E-4
5	0.0	0.0	0.0	1.8809949231947024E-4	1.8809949231947024E-4
6	0.0	0.0	0.0	1.8809949231947024E-4	2.3939935386114392E-4
7	0.0	0.0	0.0	2.564993077083685E-4	2.906992154028176E-4
8	0.0	0.0	0.0	3.077991692500422E-4	2.906992154028176E-4
9	0.0	0.0	0.0	5.300985692639616E-4	2.906992154028176E-4
10-11	0.0	0.0	0.0	6.241483154236966E-4	2.906992154028176E-4
12-13	0.0	0.0	0.0	8.207977846667791E-4	9.06297553902902E-4
14-15	0.0	0.0	0.0	0.0010943970462223723	0.0011285969539168213
16-17	0.0	0.0	0.0	0.0013679963077779652	0.0014107461923960267
18-19	0.0	0.0	0.0	0.0018638949693474778	0.001504795938555762
20-21	0.0	0.0	0.0	0.003385790861750464	0.0016843454539516199
22-23	0.0	0.0	0.0	0.005677184677278556	0.001778395200111355
24-25	0.0	0.0	0.0	0.00794292856203581	0.001778395200111355
26-27	0.0	0.0	0.0	0.010183022516022228	0.0018895449001183145
28-29	0.0	0.0	0.0	0.016347555877946687	0.001932294784736376
30-31	0.0	0.0	0.0	0.02737702610940653	0.0019664946924308254
32-33	0.0	0.0	0.0	0.06087583569611946	0.00217169413859752
34-35	0.0	0.0	0.0	0.10393351948343091	0.0022913938155280917
36-37	0.0	0.0	0.0	0.14930824701704132	0.002308493769375316
38-39	0.0	0.0	0.0	0.21901620887525225	0.0024281934463058884
40-41	0.0	0.0	0.0	0.29179361244904	0.002445293400153113
42-43	0.0	0.0	0.0	0.36763190776148097	0.0024965932616947866
44-45	0.0	0.0	0.0	0.46173295378275775	0.002624842915548971
46-47	0.0	0.0	0.0	0.5820824289595242	0.0027616425463267676
48-49	0.0	0.0	0.0	0.679868515034878	0.0030181418540351362
50-51	0.0	0.0	0.0	0.7862815278261563	0.0030950916463476463
52-53	0.0	0.0	0.0	0.8928569901789836	0.003300291092514341
54-55	0.0	0.0	0.0	1.0166093561713476	0.003419990769444913
56-57	0.0	0.0	0.0	1.197783367182692	0.0034883905848338117
58-59	0.0	0.0	0.0	1.3469548145689556	0.0035225904925282607
60-61	0.0	0.0	0.0	1.49482666546283	0.0035738903540699345
62-63	0.0	0.0	0.0	1.6465374559954062	0.0037961897540838537
64-65	0.0	0.0	0.0	1.7953925542354963	0.0038987894771672013
66-67	0.0	0.0	0.0	1.9770026140699444	0.003950089338708875
68-69	0.0	0.0	0.0	2.190726387229481	0.0039671892925560994
70-71	0.0	0.0	0.0	2.3991064248117597	0.004001389200250548
72-73	0.0	0.0	0.0	2.5806651847846664	0.004163838761799182
74-75	0.0	0.0	0.0	2.788694673313077	0.004351938254118652
76-77	0.0	0.0	0.0	2.9840359960868463	0.004445988000278387
78-79	0.0	0.0	0.0	3.2825840903055403	0.004488737884896449
80-81	0.0	0.0	0.0	3.5785842914009973	0.00456568767720896
82-83	0.0	0.0	0.0	3.817992195239065	0.004779437100299266
84-85	0.0	0.0	0.0	4.07007971485485	0.004822186984917327
86-87	1.7099953847224567E-5	0.0	0.0	4.321474686339822	0.00501883645416041
88	1.7099953847224567E-5	0.0	0.0	4.537592453037969	0.005215485923403493
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2020	0.0	28.164795	1
GAGTACG	4900	0.0	22.262014	1
CTAACGC	900	0.0	21.407822	3
GTACGCA	4935	0.0	21.330055	3
TACGCAG	4985	0.0	21.115934	4
CATGGGG	9270	0.0	20.8855	4
GCATCGT	2320	0.0	20.862282	8
TAACGCC	1100	0.0	20.078562	4
CGCAGTG	5185	0.0	19.847427	6
AGTACGC	5380	0.0	19.827806	2
CGACGAG	1250	0.0	19.359865	24-25
TAGGCAT	3010	0.0	19.202866	5
TACTAGG	3020	0.0	18.828232	2
CAATGCG	1300	0.0	18.796387	18-19
CTAGGCA	3450	0.0	18.660742	4
CGCCTAC	985	0.0	18.604845	7
CTAGTAC	2940	0.0	18.541368	3
TAGTACT	2785	0.0	18.391989	4
GTATATA	1470	0.0	18.231823	1
TCTAACG	1085	0.0	18.190754	2
>>END_MODULE