FastQCFastQC Report
Mon 16 Jan 2017
ERR1146572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5847969
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3247665.553483611147734No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT882301.5087289279406235No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC781161.3357799947297941No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC690891.181418711350898No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA578370.9890100306619273No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG556710.9519715306288389No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA541620.9261677002733769No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC445800.7623159425092711No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT291330.4981729554311933No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG139770.23900605492265778No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104250.1782670188573161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19900.026.0174941
GAGTACG49800.025.8023471
GTACGCA52350.023.6223263
TACGCAG52500.023.4652714
CGCAGTG55150.022.3272426
AGTACGC56600.021.9528582
AGTAGGC30300.020.1931612
CTAACGC8900.020.0763
GAGTACT66900.019.81047212-13
CGACGAG12100.019.80856124-25
GTATAAG23200.019.67931
TAGGCAT29900.019.3427185
CATGGGG90400.018.7768344
GTATATA15300.018.7656781
TAGTACT28500.018.4780754
GCATCGT27850.018.2249648
GTGATCG16000.018.2112548
CAATGCG13300.018.02147718-19
GTCTATA11000.017.9714241
CTAGTAC29050.017.9663723
Produced by FastQC (version 0.11.5)