##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146572_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5847969
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49910028592833	34.0	31.0	34.0	31.0	34.0
2	32.522247980452704	34.0	31.0	34.0	31.0	34.0
3	32.69837682108096	34.0	31.0	34.0	31.0	34.0
4	36.04595390297042	37.0	35.0	37.0	35.0	37.0
5	36.09160069076974	37.0	35.0	37.0	35.0	37.0
6	36.25170071865976	37.0	37.0	37.0	35.0	37.0
7	36.266582637493464	37.0	37.0	37.0	35.0	37.0
8	36.291209649025156	37.0	37.0	37.0	35.0	37.0
9	38.09335685602984	39.0	38.0	39.0	37.0	39.0
10-11	38.06121364528437	39.0	38.0	39.0	35.0	39.0
12-13	38.06392817745785	39.0	38.0	39.0	36.0	39.0
14-15	39.38523784924305	41.0	39.0	41.0	36.5	41.0
16-17	39.48987853731783	41.0	39.0	41.0	37.0	41.0
18-19	39.55665317309308	41.0	39.0	41.0	37.0	41.0
20-21	39.5702999622604	41.0	39.0	41.0	37.0	41.0
22-23	39.51561969634244	41.0	39.0	41.0	37.0	41.0
24-25	39.455001984449645	41.0	39.0	41.0	36.5	41.0
26-27	39.31118290811733	40.5	39.0	41.0	36.0	41.0
28-29	39.29666034481373	40.5	39.0	41.0	36.0	41.0
30-31	39.348520486343205	41.0	39.0	41.0	36.0	41.0
32-33	39.248896924727205	41.0	39.0	41.0	36.0	41.0
34-35	39.11736578972973	40.0	39.0	41.0	35.0	41.0
36-37	39.006244475646156	40.0	38.5	41.0	35.0	41.0
38-39	38.91029253062046	40.0	38.0	41.0	35.0	41.0
40-41	39.01082375778668	40.0	39.0	41.0	35.0	41.0
42-43	38.944355553184366	40.0	38.0	41.0	35.0	41.0
44-45	38.83676999655778	40.0	38.0	41.0	35.0	41.0
46-47	38.67203972866477	40.0	38.0	41.0	35.0	41.0
48-49	38.46649717192413	40.0	38.0	41.0	34.0	41.0
50-51	38.243300109833	40.0	38.0	41.0	34.0	41.0
52-53	38.29294597833881	40.0	38.0	41.0	34.0	41.0
54-55	38.17709934850886	40.0	37.0	41.0	34.0	41.0
56-57	37.871299249363325	40.0	37.0	41.0	33.5	41.0
58-59	37.63013603526284	39.0	36.0	41.0	33.0	41.0
60-61	37.16959375126646	39.0	35.0	41.0	32.0	41.0
62-63	37.04922375614508	39.0	35.0	40.0	32.5	41.0
64-65	36.724804372253	38.0	35.0	39.5	32.0	41.0
66-67	36.317825128689975	37.0	35.0	39.0	32.0	41.0
68-69	35.793364585208984	36.5	35.0	39.0	31.5	41.0
70-71	35.25320252210639	36.0	34.5	38.0	31.0	40.0
72-73	34.82457926846055	35.0	34.0	37.0	30.5	39.0
74-75	34.59496408069195	35.0	34.0	37.0	31.0	39.0
76-77	34.3330793990187	35.0	34.0	36.5	31.0	38.5
78-79	34.03317408146315	35.0	34.0	36.0	30.0	37.0
80-81	33.737158319409694	35.0	34.0	35.5	29.5	37.0
82-83	33.434937240604384	35.0	33.5	35.0	29.5	36.5
84-85	33.297921295410426	35.0	34.0	35.0	29.0	36.0
86-87	33.3727214525248	35.0	34.0	35.0	30.0	36.0
88-89	33.229530799496374	35.0	34.0	35.0	29.0	36.0
90-91	33.09578162948538	35.0	34.0	35.0	29.0	35.0
92-93	33.01091720903445	35.0	34.0	35.0	29.0	35.0
94-95	32.864972009940544	35.0	33.0	35.0	29.0	35.0
96-97	32.78482939974545	35.0	33.0	35.0	29.0	35.0
98-99	32.91202809043618	35.0	34.0	35.0	29.0	35.0
100	32.16683433171414	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2807892494014226
1101	2	0.28635683242148247
1101	3	0.28787708131968515
1101	4	0.19259711706376947
1101	5	0.22671935386370023
1101	6	0.20906428945566802
1101	7	0.2049529644499799
1101	8	0.19961958743376584
1101	9	0.25762084167791244
1101	10-11	0.24661290744844422
1101	12-13	0.23158221221869724
1101	14-15	0.3596309164533906
1101	16-17	0.36237023852986994
1101	18-19	0.3581699105692735
1101	20-21	0.3353332603726358
1101	22-23	0.34603748369083576
1101	24-25	0.3511076980103667
1101	26-27	0.3736256334578627
1101	28-29	0.4120278665917283
1101	30-31	0.37922870280323906
1101	32-33	0.3751143955413454
1101	34-35	0.40355515073752457
1101	36-37	0.4092486185088191
1101	38-39	0.42472938279259864
1101	40-41	0.40115307790161125
1101	42-43	0.39165172395065895
1101	44-45	0.412383683049498
1101	46-47	0.42286919907454745
1101	48-49	0.4247194391352025
1101	50-51	0.4498411544969798
1101	52-53	0.47762844299833773
1101	54-55	0.44306770037151466
1101	56-57	0.4488169824243329
1101	58-59	0.4764755901202804
1101	60-61	0.4506976701209311
1101	62-63	0.4358619669635644
1101	64-65	0.4517185356166138
1101	66-67	0.3847708444305056
1101	68-69	0.39138214174574415
1101	70-71	0.3979827748033742
1101	72-73	0.38296169670974933
1101	74-75	0.3498678677735896
1101	76-77	0.3481977874177744
1101	78-79	0.31861899251344994
1101	80-81	0.3493988396825429
1101	82-83	0.3661686750237507
1101	84-85	0.39747040192966665
1101	86-87	0.36198047470998773
1101	88-89	0.3155232366260492
1101	90-91	0.38516842051470235
1101	92-93	0.35542238375300883
1101	94-95	0.35132391949668573
1101	96-97	0.3868146905728409
1101	98-99	0.3607850732703355
1101	100	0.4487921674283797
1102	1	0.23017533571917426
1102	2	0.21045552485351493
1102	3	0.27551120557740916
1102	4	0.14071712158821015
1102	5	0.12227967957284136
1102	6	0.1529779953076158
1102	7	0.17573671181896344
1102	8	0.1816449442920316
1102	9	0.22948381057381084
1102	10-11	0.22330675527569355
1102	12-13	0.2268687019418394
1102	14-15	0.308468468395084
1102	16-17	0.30890121363290035
1102	18-19	0.26268780944795367
1102	20-21	0.2595229863623487
1102	22-23	0.2880802356930232
1102	24-25	0.2845882936465358
1102	26-27	0.30360045670587965
1102	28-29	0.3047582905187056
1102	30-31	0.3486939432896108
1102	32-33	0.32633014017097395
1102	34-35	0.33855397696020617
1102	36-37	0.39594526415643116
1102	38-39	0.3726573435483189
1102	40-41	0.3467028550473259
1102	42-43	0.36036572879355333
1102	44-45	0.37590413627824404
1102	46-47	0.40349954019257694
1102	48-49	0.3953076477295028
1102	50-51	0.3403996273990657
1102	52-53	0.35002734609299324
1102	54-55	0.3915367062048176
1102	56-57	0.4355942220014555
1102	58-59	0.4334901033821481
1102	60-61	0.37280195783054637
1102	62-63	0.38887356366566195
1102	64-65	0.3751475576740049
1102	66-67	0.3531119911446794
1102	68-69	0.2856331837568007
1102	70-71	0.30375511755942597
1102	72-73	0.32136131805082613
1102	74-75	0.2767368653278126
1102	76-77	0.2915329290762614
1102	78-79	0.27199426954940975
1102	80-81	0.3108648467099542
1102	82-83	0.3247569940057673
1102	84-85	0.36458774280566786
1102	86-87	0.33802932599684254
1102	88-89	0.3619621251306455
1102	90-91	0.3921414385240638
1102	92-93	0.3640861403960187
1102	94-95	0.3824448686713069
1102	96-97	0.3210983841096535
1102	98-99	0.36828817928665813
1102	100	0.3746967521318041
1103	1	0.20336985245609185
1103	2	0.20161617425074496
1103	3	0.22584798588380295
1103	4	0.15377906930353902
1103	5	0.09027327844315636
1103	6	0.11766365922562017
1103	7	0.14356820709105023
1103	8	0.1114105898028086
1103	9	0.18008226856266418
1103	10-11	0.19537028354142905
1103	12-13	0.16585648298659805
1103	14-15	0.24743420550110784
1103	16-17	0.21226348958247598
1103	18-19	0.2137053173355099
1103	20-21	0.24573590059417683
1103	22-23	0.24571079078574343
1103	24-25	0.26373994287993696
1103	26-27	0.2688332673129068
1103	28-29	0.2725173096214917
1103	30-31	0.2913439483946618
1103	32-33	0.2637377471686264
1103	34-35	0.293545765055363
1103	36-37	0.3187776339276951
1103	38-39	0.31804265197285275
1103	40-41	0.25284019405466296
1103	42-43	0.22883425088241438
1103	44-45	0.24678391578724046
1103	46-47	0.3314959176744807
1103	48-49	0.32791790223645734
1103	50-51	0.3143925648436863
1103	52-53	0.30532694284483597
1103	54-55	0.318499895031529
1103	56-57	0.3067321844221311
1103	58-59	0.2859941630960634
1103	60-61	0.32415923808594016
1103	62-63	0.31916855941736344
1103	64-65	0.30987782078131687
1103	66-67	0.25037347263607757
1103	68-69	0.2638018346199047
1103	70-71	0.2532483946151203
1103	72-73	0.19246653715762108
1103	74-75	0.17684878939429183
1103	76-77	0.2589855374306964
1103	78-79	0.24040659572421674
1103	80-81	0.27149335058729207
1103	82-83	0.22583094781791146
1103	84-85	0.22267330092352466
1103	86-87	0.262173794013961
1103	88-89	0.2521522067883808
1103	90-91	0.2005075111388379
1103	92-93	0.20001893799711468
1103	94-95	0.23822237263826906
1103	96-97	0.24147933347973094
1103	98-99	0.21921870501792995
1103	100	0.21577318024165493
1104	1	0.15682090335801746
1104	2	0.1877964316207965
1104	3	0.16183371866445384
1104	4	0.12225692834790891
1104	5	0.07163582431886795
1104	6	0.056602574478944234
1104	7	0.10185069545291725
1104	8	0.10847866209029888
1104	9	0.12861343147256576
1104	10-11	0.12040169705263537
1104	12-13	0.14489514093953204
1104	14-15	0.21153322543643327
1104	16-17	0.2204574557094503
1104	18-19	0.20194365008548942
1104	20-21	0.21047128292735806
1104	22-23	0.2292805026603162
1104	24-25	0.20596609885463835
1104	26-27	0.21700489187005445
1104	28-29	0.25650801939629986
1104	30-31	0.22153197158826998
1104	32-33	0.205008155963732
1104	34-35	0.24861617260079782
1104	36-37	0.2785356123756699
1104	38-39	0.2779315648651206
1104	40-41	0.27101191284977943
1104	42-43	0.2658895948976152
1104	44-45	0.26504029510901006
1104	46-47	0.2808160675251514
1104	48-49	0.2624628183590261
1104	50-51	0.30711808476328883
1104	52-53	0.3061674533729004
1104	54-55	0.23828320095902455
1104	56-57	0.2412652695818025
1104	58-59	0.25012214584313597
1104	60-61	0.25513855295334764
1104	62-63	0.3256106467515849
1104	64-65	0.30409258236078074
1104	66-67	0.2697871864531791
1104	68-69	0.28176487264318695
1104	70-71	0.25466854550035833
1104	72-73	0.2994095300470647
1104	74-75	0.2635131021602888
1104	76-77	0.27737387552253523
1104	78-79	0.2682118168971499
1104	80-81	0.2818574277276298
1104	82-83	0.31315528490755185
1104	84-85	0.31473967743966114
1104	86-87	0.34097084936412614
1104	88-89	0.3080359648635209
1104	90-91	0.24538335209750528
1104	92-93	0.3299034739452722
1104	94-95	0.31909450717650145
1104	96-97	0.2778948067812408
1104	98-99	0.24514925634387907
1104	100	0.34857445827245925
1105	1	0.16981534849170998
1105	2	0.17563281873874814
1105	3	0.22311668267440865
1105	4	0.13638914902302446
1105	5	0.09286377814591162
1105	6	0.0938752023610121
1105	7	0.12746242538907637
1105	8	0.09961222300756845
1105	9	0.09746238740890334
1105	10-11	0.12977033053002884
1105	12-13	0.13206803787907972
1105	14-15	0.17421520633732968
1105	16-17	0.19785401506801037
1105	18-19	0.1605771636735298
1105	20-21	0.1745130142733089
1105	22-23	0.19267903693723554
1105	24-25	0.20028944986812292
1105	26-27	0.20627444980669196
1105	28-29	0.2205327890983355
1105	30-31	0.22142521253493896
1105	32-33	0.1625574617327743
1105	34-35	0.21609589622882908
1105	36-37	0.24142475276885023
1105	38-39	0.2623978943682559
1105	40-41	0.24900392361117696
1105	42-43	0.21086917733878607
1105	44-45	0.27476659664129244
1105	46-47	0.23495179841167868
1105	48-49	0.24778004098160977
1105	50-51	0.2006384500918017
1105	52-53	0.22802113970191584
1105	54-55	0.23121765921292337
1105	56-57	0.23804465542166042
1105	58-59	0.2627942476675855
1105	60-61	0.23466774672941426
1105	62-63	0.22633380860190044
1105	64-65	0.2626595146893962
1105	66-67	0.25215900119179224
1105	68-69	0.27609811394478356
1105	70-71	0.2037595967900927
1105	72-73	0.1872168084854664
1105	74-75	0.14286177375284836
1105	76-77	0.137733466217405
1105	78-79	0.17002443202291317
1105	80-81	0.1692434519673114
1105	82-83	0.16882047237152165
1105	84-85	0.17842132381658615
1105	86-87	0.1385388267158021
1105	88-89	0.11708178574350825
1105	90-91	0.16210932845954318
1105	92-93	0.16590411787910853
1105	94-95	0.19889552931495302
1105	96-97	0.17522010609339134
1105	98-99	0.18715212633731682
1105	100	0.24236141291356006
1106	1	0.10682822640313816
1106	2	0.1409215681352265
1106	3	0.15394707934028418
1106	4	0.10209788627548733
1106	5	0.03681105741388535
1106	6	0.06817668095548868
1106	7	0.10481407831709078
1106	8	0.09871695056516216
1106	9	0.11793028765688263
1106	10-11	0.11063593804586702
1106	12-13	0.10534028500592285
1106	14-15	0.15135559720305736
1106	16-17	0.13570784826819704
1106	18-19	0.1635759823646623
1106	20-21	0.13768041797911934
1106	22-23	0.19657046271065326
1106	24-25	0.16525619186905516
1106	26-27	0.17399035510096184
1106	28-29	0.18490208797087604
1106	30-31	0.19745196886260175
1106	32-33	0.2036967484256067
1106	34-35	0.17827275795363562
1106	36-37	0.20993529227859398
1106	38-39	0.2359113072273331
1106	40-41	0.18636436144464597
1106	42-43	0.15476810803731667
1106	44-45	0.2029217311097753
1106	46-47	0.2473147410002312
1106	48-49	0.22124182482801302
1106	50-51	0.2003078391264097
1106	52-53	0.20589259100841417
1106	54-55	0.23560437875770646
1106	56-57	0.2478106032394436
1106	58-59	0.23117314420576207
1106	60-61	0.26417175692697725
1106	62-63	0.2499414195399936
1106	64-65	0.28633426840290355
1106	66-67	0.21435219679015205
1106	68-69	0.21487233927761196
1106	70-71	0.2111872644218451
1106	72-73	0.17129936138591972
1106	74-75	0.2104125542925459
1106	76-77	0.18544199233689795
1106	78-79	0.16624396420407095
1106	80-81	0.15318889449082462
1106	82-83	0.22507809171347049
1106	84-85	0.20290092516255243
1106	86-87	0.17156372447494306
1106	88-89	0.2499020643378742
1106	90-91	0.21256186939898214
1106	92-93	0.188041019553026
1106	94-95	0.2494232015165423
1106	96-97	0.20316170648889909
1106	98-99	0.19411137401382916
1106	100	0.28842158465599965
1107	1	0.07918912266883638
1107	2	0.07645482660992542
1107	3	0.09382434676378182
1107	4	0.06515242044587666
1107	5	0.012335108820103358
1107	6	0.03793418477991395
1107	7	0.03749938249761442
1107	8	0.05487474217336796
1107	9	0.047855437503784515
1107	10-11	0.055764258996603644
1107	12-13	0.07154053865860988
1107	14-15	0.13486328615484666
1107	16-17	0.10151810557051277
1107	18-19	0.1065659991057828
1107	20-21	0.10664595835238089
1107	22-23	0.0998469510753992
1107	24-25	0.07561234540439443
1107	26-27	0.07086691596104089
1107	28-29	0.13680723434683983
1107	30-31	0.06566232692740215
1107	32-33	0.06204667792250973
1107	34-35	0.09882158621089587
1107	36-37	0.14919480825893316
1107	38-39	0.15782150473950907
1107	40-41	0.11959666241545364
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2216	66-67	-0.9201204411491517
2216	68-69	-0.9604994417574559
2216	70-71	-0.9568524166708627
2216	72-73	-0.9196336496378379
2216	74-75	-0.9384324899200465
2216	76-77	-0.8919542550983337
2216	78-79	-1.0189175629404232
2216	80-81	-1.0233287469850083
2216	82-83	-0.9667775241632626
2216	84-85	-1.0266198693667832
2216	86-87	-0.9892140411994745
2216	88-89	-0.9600657969195439
2216	90-91	-1.0669816604116633
2216	92-93	-1.0903913692766167
2216	94-95	-1.0871333835514392
2216	96-97	-1.0826107731066266
2216	98-99	-1.06343633418507
2216	100	-1.2341584052358634
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	18.0
8	102.0
9	100.0
10	147.0
11	120.0
12	125.0
13	137.0
14	242.0
15	408.0
16	793.0
17	1591.0
18	2656.0
19	4337.0
20	6460.0
21	9537.0
22	14602.0
23	18024.0
24	20148.0
25	22417.0
26	26153.0
27	32692.0
28	42190.0
29	53263.0
30	67590.0
31	86144.0
32	112773.0
33	151367.0
34	222451.0
35	366834.0
36	764889.0
37	1711908.0
38	1782016.0
39	325393.0
40	342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.23906136171639	17.59946781767625	12.778275606144037	21.38319521446332
2	14.146998019257683	25.20052598438512	33.38960241214809	27.262873584209103
3	28.573690657226553	22.991737450694284	28.361206049364622	20.073365842714537
4	11.891307905359962	15.399705436195028	42.09228879291255	30.616697865532462
5	12.005950414693475	40.662626848748715	34.14224919311699	13.189173543440816
6	28.76059107370021	37.321980097921745	15.82829305930796	18.08913576907009
7	25.8875776829794	32.87099602306237	22.302434333162278	18.93899196079595
8	24.517965126012125	25.761354070105362	20.17751804087881	29.543162763003707
9	21.679338587465153	21.813282525950463	24.964718520224714	31.54266036635967
10-11	26.735145141843265	22.368560777254462	26.751099398782724	24.145194682119552
12-13	32.45207866183969	21.61183651965323	24.122143944333494	21.813940874173582
14-15	24.636202632470393	24.162824448222775	29.73083637441867	21.470136544888163
16-17	21.258799422500356	26.929725516670832	28.555708828141874	23.255766232686938
18-19	20.423620576648062	32.63489426842037	25.50062252381981	21.44086263111176
20-21	24.352258023255597	25.247526106927037	23.217428136161463	27.182787733655907
22-23	21.819934416238603	26.177826847276087	25.34700458272368	26.655234153761626
24-25	27.327231043803412	25.69063721097017	23.71571224129266	23.266419503933758
26-27	25.369106778780804	21.72726975809892	30.743246073978845	22.160377389141427
28-29	21.091133038199427	31.724905424397914	25.572297176095553	21.6116643613071
30-31	23.406582733210147	24.733360282874422	24.770518501646556	27.08953848226888
32-33	24.314117555321015	25.859031017799992	26.451973608159417	23.374877818719575
34-35	27.55269552917148	24.154237248428366	25.95552034435686	22.337546878043298
36-37	24.97925117496875	22.470727419990162	30.281417081265538	22.268604323775552
38-39	22.980558825709675	26.811166390942017	28.43053606976904	21.77773871357927
40-41	22.947024544629695	28.199425501010793	25.78854244223289	23.06500751212662
42-43	21.76542252589655	28.13322112271664	22.336299595848548	27.765056755538264
44-45	24.588583581818376	23.10488954439065	24.354749003387948	27.951777870403028
46-47	27.86671874190421	27.04990245297967	25.055280064856927	20.028098740259193
48-49	25.882867954985784	25.644911960300483	26.02882558443757	22.443394500276163
50-51	18.743573568648948	26.84489986950914	31.73233076586731	22.679195795974604
52-53	23.644168629768558	29.725261496116207	22.897813706418454	23.732756167696785
54-55	21.241833362460707	24.665485497474467	26.142764948242764	27.949916191822062
56-57	25.894558298907956	22.41012778231691	25.724412157907935	25.97090176086721
58-59	30.2870556891286	23.35640460889699	23.683278555085487	22.673261146888926
60-61	24.513200419264233	25.33779216973857	30.229972023115792	19.919035387881404
62-63	21.668324381558914	26.151379394636663	28.356406678318123	23.8238895454863
64-65	20.56533865417309	31.913136424526673	24.668089505640552	22.853435415659682
66-67	24.30915852380049	25.051261558914007	22.720296020379273	27.919283896906233
68-69	21.771286934765357	25.998059598723238	25.168109256203163	27.062544210308236
70-71	26.97282481094402	25.427954891617823	23.432750141034234	24.16647015640393
72-73	25.203238894090386	21.795294711145882	30.281051871042763	22.720414523720965
74-75	21.53738170364535	31.25275051876697	24.874590879705146	22.33527689788253
76-77	23.2419356507394	24.6932350757585	24.316868708434402	27.747960565067697
78-79	24.278294305476635	25.629578873981966	26.06400706439575	24.02811975614565
80-81	27.201795085368985	24.066825274492828	25.504504385883166	23.226875254255024
82-83	24.599313264796614	22.674291547821802	29.776648609087612	22.94974657829397
84-85	22.75991928473574	26.85773827799399	27.606426133400774	22.775916303869494
86-87	22.7756889230584	28.220563108416464	25.211842814461367	23.79190515406377
88-89	21.65863452235745	27.834992401625115	22.236716352930536	28.2696567230869
90-91	24.248187704141387	23.136818953725644	24.09477546820101	28.52021787393196
92-93	27.368493232436762	26.88487233772956	24.766701054673852	20.97993337515982
94-95	24.9244588099269	25.528557481115023	25.98687903329165	23.56010467566643
96-97	18.96127289821076	26.452706642639995	31.126638310271677	23.459382148877573
98-99	23.511164014821627	29.127089346489644	23.13551898009915	24.22622765858958
100	22.80837054876835	20.694392479255917	23.915035263747935	32.5822017082278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	185.0
1	125.0
2	149.5
3	262.5
4	190.5
5	116.0
6	218.5
7	477.0
8	854.5
9	1067.0
10	844.5
11	564.0
12	567.5
13	662.5
14	792.0
15	924.5
16	1092.0
17	1304.0
18	1499.5
19	1697.5
20	1927.0
21	2211.0
22	2523.0
23	3046.0
24	3651.0
25	4348.0
26	5576.5
27	7062.5
28	8745.0
29	11565.5
30	15711.0
31	20349.5
32	27120.5
33	35555.5
34	43872.0
35	51937.0
36	60795.5
37	72352.0
38	81645.5
39	90070.0
40	96630.5
41	100733.0
42	104798.0
43	113703.5
44	127303.5
45	144039.0
46	172937.5
47	407979.5
48	911077.5
49	919499.5
50	488262.5
51	274159.5
52	192674.5
53	167770.0
54	157973.0
55	143012.5
56	130862.5
57	117532.0
58	102560.0
59	92078.0
60	76022.5
61	58116.0
62	44808.5
63	35386.0
64	26671.5
65	18345.5
66	13587.0
67	10075.0
68	7457.5
69	6154.0
70	5164.5
71	3984.0
72	3094.5
73	2676.5
74	2040.0
75	1605.0
76	1191.5
77	792.0
78	578.5
79	384.0
80	272.5
81	189.0
82	117.5
83	59.0
84	25.5
85	12.0
86	5.0
87	3.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005967883892681374
2	0.08909075954404
3	2.7359926155559307E-4
4	0.0
5	0.03977449264864434
6	0.004069789015639447
7	3.4199907694449134E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.4199907694449134E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.702487307986755E-4
24-25	0.0
26-27	0.0
28-29	3.4199907694449134E-5
30-31	5.12998615416737E-5
32-33	0.001727095338569681
34-35	0.0014021962154724143
36-37	8.122478077431669E-4
38-39	7.780479000487178E-4
40-41	0.0015560958000974355
42-43	6.412482692709213E-4
44-45	5.386485461875739E-4
46-47	3.419990769444913E-4
48-49	6.668982000417581E-4
50-51	8.549976923612284E-4
52-53	0.0013850962616251898
54-55	0.0015560958000974355
56-57	9.40497461597351E-4
58-59	0.0025478931232364604
60-61	0.0018040451308821918
62-63	0.0010174472539098618
64-65	0.004625537515674245
66-67	0.004505837838743674
68-69	0.0035824403309935465
70-71	0.0016757954770280075
72-73	0.005027386431084023
74-75	0.003565340377146322
76-77	0.0035824403309935465
78-79	8.891976000556774E-4
80-81	3.4199907694449134E-5
82-83	1.538995846250211E-4
84-85	7.694979231251055E-5
86-87	1.7099953847224567E-5
88-89	1.3679963077779653E-4
90-91	0.0
92-93	0.0
94-95	8.549976923612283E-6
96-97	3.93298938486165E-4
98-99	4.7879870772228784E-4
100	6.32698292347309E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5847969.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.126750492822346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.07288133750369	31.62161432430677
2	15.492377700897409	13.982413260979243
3	6.064490619069404	8.2101226509842
4	2.950897809643661	5.326577167424218
5	1.7163627728686601	3.872693730320637
6	1.060476066344526	2.8713503309723487
7	0.6804990332029511	2.1496097057369483
8	0.4611619672337963	1.6648592825710913
9	0.32326989854531996	1.312930804814518
>10	1.1562184799085833	7.901133591156005
>50	0.015964470490173008	0.4705378955885629
>100	0.004130036772562397	0.3444944470442751
>500	4.232691800610638E-4	0.1305059602325695
>1k	2.1163458478441546E-4	0.29448600131773855
>5k	5.2908646196103865E-5	0.12546813174353139
>10k+	5.819951081571425E-4	19.72120271480733
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	324766	5.553483611147734	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	88230	1.5087289279406235	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	78116	1.3357799947297941	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	69089	1.181418711350898	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	57837	0.9890100306619273	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	55671	0.9519715306288389	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	54162	0.9261677002733769	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	44580	0.7623159425092711	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	29133	0.4981729554311933	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	13977	0.23900605492265778	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	10425	0.1782670188573161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.12998615416737E-5
2	0.0	0.0	0.0	3.4199907694449134E-5	5.12998615416737E-5
3	0.0	0.0	0.0	8.549976923612283E-5	5.12998615416737E-5
4	0.0	0.0	0.0	1.538995846250211E-4	5.12998615416737E-5
5	0.0	0.0	0.0	1.7099953847224565E-4	6.839981538889827E-5
6	0.0	0.0	0.0	1.7099953847224565E-4	1.025997230833474E-4
7	0.0	0.0	0.0	3.248991230972668E-4	1.025997230833474E-4
8	0.0	0.0	0.0	3.761989846389405E-4	1.025997230833474E-4
9	0.0	0.0	0.0	5.984983846528598E-4	1.025997230833474E-4
10-11	0.0	0.0	0.0	6.754481769653705E-4	1.1969967693057196E-4
12-13	0.0	0.0	0.0	9.06297553902902E-4	4.7879870772228784E-4
14-15	0.0	0.0	0.0	0.0011969967693057195	7.267480385070441E-4
16-17	0.0	0.0	0.0	0.0014705960308613129	9.917973231390249E-4
18-19	0.0	0.0	0.0	0.0019750446693544373	0.0010772970923751477
20-21	0.0	0.0	0.0	0.003565340377146322	0.0012397466539237811
22-23	0.0	0.0	0.0	0.005908034054216088	0.0013508963539307407
24-25	0.0	0.0	0.0	0.008233627777438628	0.0013679963077779652
26-27	0.0	0.0	0.0	0.01052502159296672	0.001479146007784925
28-29	0.0	0.0	0.0	0.01666390502412034	0.0015218958924029865
30-31	0.0	0.0	0.0	0.027582225555573225	0.001538995846250211
32-33	0.0	0.0	0.0	0.060995535373050025	0.0016415955693335584
34-35	0.0	0.0	0.0	0.10367702017572256	0.0017099953847224565
36-37	0.0	0.0	0.0	0.14896624794009683	0.0017099953847224565
38-39	0.0	0.0	0.0	0.2185117602367591	0.0017612952462641304
40-41	0.0	0.0	0.0	0.29140886348747747	0.0017612952462641304
42-43	0.0	0.0	0.0	0.367520758061474	0.001778395200111355
44-45	0.0	0.0	0.0	0.46189540334430634	0.0019066448539655392
46-47	0.0	0.0	0.0	0.5821251788441424	0.002051994461666948
48-49	0.0	0.0	0.0	0.6800053146656557	0.0021631441616739074
50-51	0.0	0.0	0.0	0.7864012275030869	0.0023597936309169903
52-53	0.0	0.0	0.0	0.8929595899020668	0.0025478931232364604
54-55	0.0	0.0	0.0	1.016532406379035	0.002599192984778134
56-57	0.0	0.0	0.0	1.1979800166519352	0.0026761427770906447
58-59	0.0	0.0	0.0	1.3474165133228304	0.0027359926155559305
60-61	0.0	0.0	0.0	1.495245614332087	0.0027958424540212167
62-63	8.549976923612283E-6	0.0	0.0	1.6466486056954133	0.002992491923264299
64-65	1.7099953847224567E-5	0.0	0.0	1.7954438540970377	0.003052341761729585
66-67	1.7099953847224567E-5	0.0	0.0	1.9771565136545695	0.003120741577118483
68-69	1.7099953847224567E-5	0.0	0.0	2.1913932854295224	0.0031549414848129325
70-71	1.7099953847224567E-5	0.0	0.0	2.400577020842621	0.0032489912309726678
72-73	1.7099953847224567E-5	0.0	0.0	2.582811228992493	0.0034285407463685255
74-75	1.7099953847224567E-5	0.0	0.0	2.79154181562864	0.003608090261764383
76-77	1.7099953847224567E-5	0.0	0.0	2.9877381360947703	0.003710689984847731
78-79	1.7099953847224567E-5	0.0	0.0	3.2872352777519853	0.0038132897079310784
80-81	1.7099953847224567E-5	0.0	0.0	3.584261476078276	0.0038987894771672013
82-83	1.7099953847224567E-5	0.0	0.0	3.8240712288317535	0.004086888969486671
84-85	1.7099953847224567E-5	0.0	0.0	4.076688847016802	0.004138188831028345
86-87	1.7099953847224567E-5	0.0	0.0	4.328947366171059	0.004394688138736714
88	1.7099953847224567E-5	0.0	0.0	4.5456978311615535	0.004651187446445082
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1990	0.0	26.017494	1
GAGTACG	4980	0.0	25.802347	1
GTACGCA	5235	0.0	23.622326	3
TACGCAG	5250	0.0	23.465271	4
CGCAGTG	5515	0.0	22.327242	6
AGTACGC	5660	0.0	21.952858	2
AGTAGGC	3030	0.0	20.193161	2
CTAACGC	890	0.0	20.076	3
GAGTACT	6690	0.0	19.810472	12-13
CGACGAG	1210	0.0	19.808561	24-25
GTATAAG	2320	0.0	19.6793	1
TAGGCAT	2990	0.0	19.342718	5
CATGGGG	9040	0.0	18.776834	4
GTATATA	1530	0.0	18.765678	1
TAGTACT	2850	0.0	18.478075	4
GCATCGT	2785	0.0	18.224964	8
GTGATCG	1600	0.0	18.211254	8
CAATGCG	1330	0.0	18.021477	18-19
GTCTATA	1100	0.0	17.971424	1
CTAGTAC	2905	0.0	17.966372	3
>>END_MODULE