FastQCFastQC Report
Mon 16 Jan 2017
ERR1146571_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146571_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4110997
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64950.036.265511
GGTATCA40200.034.5009571
CAACGCA62850.034.39355
ATCAACG64900.033.6695563
TATCAAC78350.029.748962
GTACTAG15150.029.1709381
AACGCAG77550.028.903116
TCAACGC78850.027.4740754
ACGCAGA79250.027.333417
CGCAGAG83000.025.9292588
GTACATG141600.025.3667771
ACATGGG136100.024.8256073
TACATGG143950.024.4185052
GCTATTG24500.022.4399818
CATGGGG112500.021.8460544
GCAGAGT96600.021.8409719
TAGGCAT27250.020.8661695
CTATTGA28800.020.2316789
GGGCTAT32200.019.9927566
GTGATCG13300.019.7851378
Produced by FastQC (version 0.11.5)