##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146571_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4110997
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.650366322330083	33.0	31.0	34.0	30.0	34.0
2	31.79458924440957	33.0	31.0	34.0	30.0	34.0
3	31.83295414713268	34.0	31.0	34.0	30.0	34.0
4	35.338611777143115	37.0	35.0	37.0	33.0	37.0
5	35.317713926816296	37.0	35.0	37.0	33.0	37.0
6	35.4122518211519	37.0	35.0	37.0	33.0	37.0
7	35.27834756386346	37.0	35.0	37.0	33.0	37.0
8	35.297086083984006	37.0	35.0	37.0	33.0	37.0
9	36.92448133627925	39.0	38.0	39.0	33.0	39.0
10-11	36.90041199251665	39.0	37.5	39.0	33.0	39.0
12-13	36.89126457645189	39.0	38.0	39.0	33.0	39.0
14-15	38.33773887453579	41.0	39.0	41.0	34.0	41.0
16-17	38.353958784207336	41.0	39.0	41.0	33.5	41.0
18-19	38.38436929046652	41.0	39.0	41.0	33.5	41.0
20-21	38.46928956649688	41.0	39.0	41.0	34.0	41.0
22-23	38.49402723962095	41.0	39.0	41.0	34.0	41.0
24-25	38.54901365775747	41.0	39.0	41.0	34.0	41.0
26-27	38.574684802737636	41.0	39.0	41.0	34.0	41.0
28-29	38.53725191237065	40.5	39.0	41.0	34.0	41.0
30-31	38.526379367340816	40.0	39.0	41.0	34.0	41.0
32-33	38.5375455151147	40.0	38.5	41.0	34.0	41.0
34-35	38.48170601924545	40.0	38.0	41.0	33.5	41.0
36-37	38.39775874319538	40.0	38.0	41.0	33.0	41.0
38-39	38.35985881283786	40.0	38.0	41.0	33.0	41.0
40-41	38.29266975869844	40.0	38.0	41.0	33.0	41.0
42-43	38.182260045434234	40.0	38.0	41.0	33.0	41.0
44-45	38.05972091441565	40.0	38.0	41.0	33.0	41.0
46-47	37.95830828871926	40.0	38.0	41.0	33.0	41.0
48-49	37.83709924867374	40.0	37.0	41.0	32.5	41.0
50-51	37.705501001338604	40.0	37.0	41.0	32.0	41.0
52-53	37.56784911300106	40.0	36.5	41.0	32.0	41.0
54-55	37.37787816434796	40.0	36.0	41.0	32.0	41.0
56-57	37.23507849798966	39.5	35.5	41.0	31.5	41.0
58-59	36.997985646790795	39.0	35.0	41.0	31.0	41.0
60-61	36.803570520727696	39.0	35.0	41.0	31.0	41.0
62-63	36.54978597649183	38.5	35.0	41.0	31.0	41.0
64-65	36.289278367267116	37.5	35.0	40.0	31.0	41.0
66-67	36.02191050005631	37.0	35.0	40.0	30.5	41.0
68-69	35.72252473061887	36.5	35.0	39.5	30.0	41.0
70-71	35.45404557580558	36.0	35.0	39.0	30.0	41.0
72-73	35.13382386316506	35.5	35.0	39.0	30.0	41.0
74-75	34.81768352056691	35.0	34.0	38.5	30.0	40.0
76-77	34.513377046979116	35.0	34.0	37.0	29.5	39.0
78-79	34.23802230943005	35.0	34.0	37.0	29.0	39.0
80-81	33.97044828784842	35.0	34.0	36.0	29.0	39.0
82-83	33.72174937125958	35.0	34.0	36.0	29.0	37.0
84-85	33.50205716520834	35.0	34.0	36.0	29.0	37.0
86-87	33.3145727180049	35.0	34.0	35.0	29.0	37.0
88-89	33.178866099877965	35.0	34.0	35.0	29.0	36.0
90-91	33.021698142810614	35.0	34.0	35.0	28.5	36.0
92-93	32.93076728102696	35.0	34.0	35.0	28.0	36.0
94-95	32.84003199223935	35.0	34.0	35.0	28.0	36.0
96-97	32.76491663701044	35.0	34.0	35.0	27.5	35.0
98-99	32.69194261148816	35.0	34.0	35.0	27.0	35.0
100	31.802011531509265	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14019885219746087
1101	2	0.15666050206577964
1101	3	0.20977313456992874
1101	4	0.15556430667928112
1101	5	0.15418760341492543
1101	6	0.18305520615925275
1101	7	0.13737790980943032
1101	8	0.21240576627295837
1101	9	0.29666193344503
1101	10-11	0.2832547918210011
1101	12-13	0.27463585261256185
1101	14-15	0.36172618859057337
1101	16-17	0.34919081041002187
1101	18-19	0.26874765964996783
1101	20-21	0.3365049430948517
1101	22-23	0.30682556792164206
1101	24-25	0.31986779286268074
1101	26-27	0.2599327783490111
1101	28-29	0.3612435309171218
1101	30-31	0.3599395051746015
1101	32-33	0.3558001939726836
1101	34-35	0.4072578802236464
1101	36-37	0.3538952207197852
1101	38-39	0.3804747569985736
1101	40-41	0.3415255410610598
1101	42-43	0.36608415648377957
1101	44-45	0.25501113563684186
1101	46-47	0.2647298265753548
1101	48-49	0.3359110151943554
1101	50-51	0.3392284025575094
1101	52-53	0.26351391963899573
1101	54-55	0.2409191606065093
1101	56-57	0.27462261297578294
1101	58-59	0.2891060107370862
1101	60-61	0.23315306436555971
1101	62-63	0.21551076679596548
1101	64-65	0.2336200974617313
1101	66-67	0.2087191715415173
1101	68-69	0.19373872056509356
1101	70-71	0.15015290199873732
1101	72-73	0.17030402713023562
1101	74-75	0.18523039769370797
1101	76-77	0.1897961298962798
1101	78-79	0.20457965107055287
1101	80-81	0.2342776324291833
1101	82-83	0.17884403909194901
1101	84-85	0.25330201505384053
1101	86-87	0.2384125773252066
1101	88-89	0.23776502462909832
1101	90-91	0.28937012796355077
1101	92-93	0.2319869275441775
1101	94-95	0.1590480271601038
1101	96-97	0.18633534010348995
1101	98-99	0.23378259297192727
1101	100	0.11060725236157154
1102	1	0.26000983002370504
1102	2	0.29425046935039845
1102	3	0.349094108761129
1102	4	0.27021934121218294
1102	5	0.29802911559231404
1102	6	0.27847977904183807
1102	7	0.28228848778108073
1102	8	0.2990404445717587
1102	9	0.39067647361223834
1102	10-11	0.4127949590329223
1102	12-13	0.43523890604767246
1102	14-15	0.49215130672942564
1102	16-17	0.4822845944885472
1102	18-19	0.37220749243804363
1102	20-21	0.40682828006322325
1102	22-23	0.45572978457013136
1102	24-25	0.4595880763959457
1102	26-27	0.41448439594260833
1102	28-29	0.4377651594158465
1102	30-31	0.476676146395981
1102	32-33	0.412130619272574
1102	34-35	0.43740909796265015
1102	36-37	0.4879357514358915
1102	38-39	0.4282295734289647
1102	40-41	0.4528272495307135
1102	42-43	0.3806008776760734
1102	44-45	0.3975934693336711
1102	46-47	0.40816348706244554
1102	48-49	0.3913468567065337
1102	50-51	0.384541924913016
1102	52-53	0.367221662278034
1102	54-55	0.43874904065013
1102	56-57	0.39893740759592333
1102	58-59	0.47119834079887823
1102	60-61	0.4482995565502179
1102	62-63	0.4456488983844764
1102	64-65	0.3616557208714042
1102	66-67	0.3537755146894668
1102	68-69	0.38082468930373636
1102	70-71	0.356319386913313
1102	72-73	0.37141889445121024
1102	74-75	0.3603594416777156
1102	76-77	0.3792059808595667
1102	78-79	0.30391516542896824
1102	80-81	0.33110078764546813
1102	82-83	0.294153562901478
1102	84-85	0.25555592272377936
1102	86-87	0.2299258452277826
1102	88-89	0.234664152219068
1102	90-91	0.2754137484651906
1102	92-93	0.28270921035043983
1102	94-95	0.18951022272535312
1102	96-97	0.1734543877225434
1102	98-99	0.14475569366258156
1102	100	0.11076065112565558
1103	1	0.08279649774486231
1103	2	0.06830788637515539
1103	3	0.13925985714204714
1103	4	0.12500643072966255
1103	5	0.09301508207708764
1103	6	0.2318140614169053
1103	7	0.09391445988979541
1103	8	0.18204848506420745
1103	9	0.23052729831193375
1103	10-11	0.2737949678203435
1103	12-13	0.25861296030123526
1103	14-15	0.2942599544328317
1103	16-17	0.26487421302648784
1103	18-19	0.23629476912765313
1103	20-21	0.24772206647611483
1103	22-23	0.22171277211835871
1103	24-25	0.24493951182155627
1103	26-27	0.2699878186440401
1103	28-29	0.24567239937401553
1103	30-31	0.24857686608596197
1103	32-33	0.255809737718252
1103	34-35	0.2387232219883444
1103	36-37	0.24983706867066502
1103	38-39	0.23738726374258334
1103	40-41	0.19291473711847829
1103	42-43	0.19574071194527676
1103	44-45	0.20091901700983783
1103	46-47	0.13587362511287182
1103	48-49	0.0821526107114039
1103	50-51	0.13811902137256737
1103	52-53	0.1669527671207831
1103	54-55	0.1262417251201171
1103	56-57	0.17982335562710006
1103	58-59	0.17488322379433896
1103	60-61	0.10543422381443435
1103	62-63	0.11380028811549892
1103	64-65	0.10442609670950276
1103	66-67	0.09662650635019787
1103	68-69	0.12722348015427087
1103	70-71	0.05877910975797107
1103	72-73	0.045681339639656926
1103	74-75	0.06531932708971055
1103	76-77	0.08478427240870445
1103	78-79	0.04507696825675822
1103	80-81	0.08332838191255121
1103	82-83	0.008178325860733082
1103	84-85	-0.0011248213952441688
1103	86-87	0.04392269933019577
1103	88-89	-0.03139985537326595
1103	90-91	0.012464654009178844
1103	92-93	0.031382854231310375
1103	94-95	0.05269719273387352
1103	96-97	0.0027613412107072577
1103	98-99	-0.01766654203368745
1103	100	-0.08160244521086568
1104	1	0.18637456370725403
1104	2	0.13266430114516936
1104	3	0.1423956167259739
1104	4	0.19176375562199865
1104	5	0.17954986498454417
1104	6	0.18288385241699245
1104	7	0.11939421532736105
1104	8	0.17401244436774732
1104	9	0.2294115512205792
1104	10-11	0.3484473620862545
1104	12-13	0.3045555786646048
1104	14-15	0.3506397377319601
1104	16-17	0.2969369125382215
1104	18-19	0.2165415228013572
1104	20-21	0.2304624923343539
1104	22-23	0.2642954668944739
1104	24-25	0.22228476635361005
1104	26-27	0.2799318721840862
1104	28-29	0.28515204505193026
1104	30-31	0.23425201215820124
1104	32-33	0.26210103800057993
1104	34-35	0.27707918177625146
1104	36-37	0.2772445357136135
1104	38-39	0.2637490987685496
1104	40-41	0.26265901029642436
1104	42-43	0.23746988704347416
1104	44-45	0.21509191376678416
1104	46-47	0.1976050810509875
1104	48-49	0.17025262165442712
1104	50-51	0.16181585769212603
1104	52-53	0.11875427818052486
1104	54-55	0.17416955373219167
1104	56-57	0.16348850329303843
1104	58-59	0.17536936815305637
1104	60-61	0.15819605609970466
1104	62-63	0.1444739778629014
1104	64-65	0.1328829016401727
1104	66-67	0.1337741546309772
1104	68-69	0.18639997704110556
1104	70-71	0.14510835683901746
1104	72-73	0.14996852808545924
1104	74-75	0.15584353749383695
1104	76-77	0.15776153074821053
1104	78-79	0.1229149784209227
1104	80-81	0.12416350334224546
1104	82-83	0.1508495728299053
1104	84-85	0.15725687986493142
1104	86-87	0.17163224260286825
1104	88-89	0.1192500654133326
1104	90-91	0.19415236222558008
1104	92-93	0.18390942795979015
1104	94-95	0.11930155575266355
1104	96-97	0.1315468716993493
1104	98-99	0.09732021993720963
1104	100	0.04494381700178707
1105	1	0.1310527810346933
1105	2	0.19281403329489066
1105	3	0.14706899269445728
1105	4	0.14262028496741408
1105	5	0.11726102060791987
1105	6	0.12934879494868312
1105	7	0.11234130763038053
1105	8	0.143844869058654
1105	9	0.20737667910733393
1105	10-11	0.23977367380066283
1105	12-13	0.26287804652005775
1105	14-15	0.23658254198324613
1105	16-17	0.16365121295265794
1105	18-19	0.21577610496564148
1105	20-21	0.2439588582401555
1105	22-23	0.21329210539617094
1105	24-25	0.23717043933578452
1105	26-27	0.2878289427117693
1105	28-29	0.2865458378033452
1105	30-31	0.2943053419207544
1105	32-33	0.2643826932508091
1105	34-35	0.2683911107997474
1105	36-37	0.2707874144239426
1105	38-39	0.2907073875700732
1105	40-41	0.2894669735076718
1105	42-43	0.23053451037262818
1105	44-45	0.22364699953637768
1105	46-47	0.22811015981785943
1105	48-49	0.20809932391611596
1105	50-51	0.22333127975991118
1105	52-53	0.27031677352444916
1105	54-55	0.26667455502209947
1105	56-57	0.2043721185074574
1105	58-59	0.23460468831391523
1105	60-61	0.22253260320521662
1105	62-63	0.19713125371339402
1105	64-65	0.1581916825123173
1105	66-67	0.19709438725713824
1105	68-69	0.24478156070439638
1105	70-71	0.2135475372037945
1105	72-73	0.1469430591517238
1105	74-75	0.16146277272777354
1105	76-77	0.1651925348844756
1105	78-79	0.15529847719800216
1105	80-81	0.09093243388947769
1105	82-83	0.1411442521979751
1105	84-85	0.1894094377046187
1105	86-87	0.16210038920627312
1105	88-89	0.0926164570621566
1105	90-91	0.15273439691636526
1105	92-93	0.12786982632831467
1105	94-95	0.14965843288847935
1105	96-97	0.10112179011237288
1105	98-99	0.07214225276145214
1105	100	0.01781537370728259
1106	1	0.07290009078702653
1106	2	0.044870706641805924
1106	3	0.0477815464640976
1106	4	0.07332644387768994
1106	5	0.009604048874201965
1106	6	0.02575677978136781
1106	7	0.013034315889498771
1106	8	-0.03092425109718988
1106	9	0.08501800603885101
1106	10-11	1.8153824259314888E-4
1106	12-13	-0.00725873068425642
1106	14-15	0.049666756393421
1106	16-17	0.040037850010875786
1106	18-19	0.02683779371453454
1106	20-21	0.09352896492866591
1106	22-23	0.07311594578681735
1106	24-25	0.0739281940770411
1106	26-27	0.06394795016916532
1106	28-29	0.16247864129745437
1106	30-31	0.09657486347903443
1106	32-33	0.07967860808322769
1106	34-35	0.07223125802180874
1106	36-37	0.11020085545183633
1106	38-39	0.09643602981299892
1106	40-41	0.11195827425660809
1106	42-43	0.08724461903487679
1106	44-45	0.08730621168922426
1106	46-47	0.07285397932957949
1106	48-49	0.0879891190132156
1106	50-51	0.08478645081250846
1106	52-53	0.038623707228360615
1106	54-55	0.07788910045528041
1106	56-57	0.026351638649586562
1106	58-59	0.011380778662612556
1106	60-61	0.028579354627446207
1106	62-63	0.06184821673437568
1106	64-65	0.053689706916863145
1106	66-67	0.0331001344766122
1106	68-69	0.03159681881725618
1106	70-71	0.023968690794212932
1106	72-73	0.03977547462548614
1106	74-75	0.026222918736330314
1106	76-77	0.0503595129003358
1106	78-79	0.020157504903579593
1106	80-81	0.04850704467899902
1106	82-83	0.05364991350113968
1106	84-85	0.09767714069104017
1106	86-87	0.028337375403665988
1106	88-89	0.028831317261996503
1106	90-91	0.02776717761537384
1106	92-93	-0.02895639525710436
1106	94-95	-0.0370231363306317
1106	96-97	-0.06794386313756462
1106	98-99	-0.054596803041704334
1106	100	-0.08333510142249523
1107	1	0.03674095915658171
1107	2	0.056507348812520064
1107	3	0.09748642871677049
1107	4	0.06834794267702904
1107	5	0.0473360170040209
1107	6	0.014725984074068776
1107	7	-0.030808344547196498
1107	8	0.03994310393729705
1107	9	0.07483571207716011
1107	10-11	0.07890387518550313
1107	12-13	0.09609091963331196
1107	14-15	0.1347514777051444
1107	16-17	0.11445122649796957
1107	18-19	0.14621437767637957
1107	20-21	0.12529291812612087
1107	22-23	0.11359494480210941
1107	24-25	0.11087044048179706
1107	26-27	0.08714320696823563
1107	28-29	0.08389357604207248
1107	30-31	0.07970218553555952
1107	32-33	0.1185828731484122
1107	34-35	0.07180003162869042
1107	36-37	0.10630760051515864
1107	38-39	0.12908639281852885
1107	40-41	0.09332779249413647
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2216	38-39	-0.47229110518781425
2216	40-41	-0.39703277844262885
2216	42-43	-0.34327504477106174
2216	44-45	-0.2872481887010494
2216	46-47	-0.20768137210526305
2216	48-49	-0.23461162805150337
2216	50-51	-0.3118691778099887
2216	52-53	-0.2559529018821465
2216	54-55	-0.2533157779035662
2216	56-57	-0.26109337477970485
2216	58-59	-0.25790430028213507
2216	60-61	-0.27591924410756263
2216	62-63	-0.24244206763201248
2216	64-65	-0.27199247051772346
2216	66-67	-0.3009872678968364
2216	68-69	-0.24067482204068824
2216	70-71	-0.23606916157478253
2216	72-73	-0.2109384971228394
2216	74-75	-0.21903515209441338
2216	76-77	-0.19380623260392582
2216	78-79	-0.1993570362131507
2216	80-81	-0.18560594292681287
2216	82-83	-0.15057865969138362
2216	84-85	-0.1564467224537509
2216	86-87	-0.1614132817465901
2216	88-89	-0.1796239848619905
2216	90-91	-0.13463214419444114
2216	92-93	-0.09575204540663407
2216	94-95	-0.14902438403857587
2216	96-97	-0.11277520432319932
2216	98-99	-0.05964702876348582
2216	100	-0.07812225295154462
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	116.0
1	59.0
2	49.0
3	54.0
4	46.0
5	65.0
6	234.0
7	1146.0
8	3242.0
9	3670.0
10	3423.0
11	3672.0
12	4369.0
13	5294.0
14	6408.0
15	7163.0
16	7663.0
17	8456.0
18	9577.0
19	10814.0
20	12354.0
21	13665.0
22	15633.0
23	18313.0
24	23788.0
25	30531.0
26	35047.0
27	33240.0
28	35302.0
29	44592.0
30	51064.0
31	56020.0
32	71731.0
33	97053.0
34	147289.0
35	233040.0
36	447941.0
37	1014373.0
38	1254086.0
39	399757.0
40	658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61318122992391	19.902624674663063	13.038328725331533	24.44586537008149
2	15.303452416516405	20.669956026183833	39.72933089542522	24.297260661874535
3	17.838600807047055	26.31283813979717	30.391692966935498	25.45686808622028
4	12.54286775651382	17.579617803404705	39.35888300681627	30.51863143326521
5	13.341908060981932	38.464611245900464	35.21033424165114	12.983146451466466
6	30.1707817278148	38.315683455337656	18.390491690812087	13.123043126035455
7	26.517168527791597	32.94211802138966	23.180693114466177	17.36002033635257
8	25.88690455628865	34.68323846842845	21.00914235282155	18.420714622461357
9	26.512073256676423	15.40648702640939	20.787569601943417	37.293870114970765
10-11	24.563216703312847	26.277995346540877	28.868268476056	20.290519474090274
12-13	25.225506733334274	24.219544792643273	28.092300611422466	22.462647862599987
14-15	22.742404375041055	25.515911341194332	25.490002651687472	26.251681632077144
16-17	22.533481973163333	28.14350433603792	26.38821634028547	22.934797350513275
18-19	22.393385119681785	28.029435093512095	27.597924086125587	21.979255700680532
20-21	23.638592184120107	27.2614839098277	26.790774241322907	22.30914966472929
22-23	23.66568204446587	27.092695170356347	26.454766530694258	22.786856254483524
24-25	23.27322427434728	27.277874426196053	26.812993034137723	22.635908265318943
26-27	23.114768155736584	27.495279189206446	26.428646579274652	22.961306075782318
28-29	22.90532114202698	27.699332249749954	26.59380231337713	22.801544294845932
30-31	23.17845987560804	27.351476432293744	26.566950535993765	22.903113156104453
32-33	23.238178785649506	27.597312396121616	26.072343632619855	23.092165185609023
34-35	23.46036586107555	27.445796421962953	26.562064380144417	22.531773336817075
36-37	23.391634664616202	27.15551129259089	26.474645848637934	22.978208194154977
38-39	23.28715498376684	27.18502323271803	26.700102511775714	22.827719271739415
40-41	23.21601961529937	26.821046157409466	27.00535318803356	22.957581039257605
42-43	23.1035427461392	26.950824227159302	26.707079166293596	23.238553860407897
44-45	23.042683834071614	27.36448727292989	26.677837305041518	22.914991587956976
46-47	23.149453753285158	27.15587941231791	26.420619824987902	23.274047009409035
48-49	23.343198163864063	26.912579757611077	26.567105889881727	23.177116188643136
50-51	23.19209695292072	27.07373714810743	26.45257349309172	23.28159240588013
52-53	23.084407127365296	27.177268386724258	26.426094140894413	23.312230345016037
54-55	23.025380851475955	27.11625521112667	26.464675788348686	23.393688149048685
56-57	23.33502337156178	27.02497667224051	26.391327925941038	23.248672030256675
58-59	23.070001952795874	26.943777120897533	26.764363239499765	23.22185768680683
60-61	23.09867596492033	27.118731712100818	26.594792411774286	23.187799911204564
62-63	23.25218354252118	26.965127436832724	26.42533871690468	23.357350303741413
64-65	22.76400023311467	27.073020249950996	26.48175336297718	23.68122615395715
66-67	23.091067682221812	26.91784464517561	26.434936420733884	23.556151251868698
68-69	22.931916405474333	26.85970564759476	26.68969133879728	23.518686608133628
70-71	22.93430288718631	26.721673322372236	26.55449487958224	23.789528910859215
72-73	23.154104176027563	26.78785370197533	26.65757070389441	23.400471418102693
74-75	23.14072955776495	27.04480752884652	26.52474643021486	23.289716483173663
76-77	23.070050479290447	26.810771770532398	26.5296242183789	23.589553531798256
78-79	22.91997204501609	26.902389216695898	26.5566141959146	23.62102454237341
80-81	22.9946727725407	27.004037459722134	26.691597309203807	23.30969245853336
82-83	23.258770012426343	26.5688757926766	27.087879042652176	23.084475152244888
84-85	23.290579910543027	26.924046984437677	26.56958197730251	23.21579112771678
86-87	22.884348586864757	27.15046591080943	26.654084051431777	23.31110145089404
88-89	23.21979201834667	26.5916375437604	26.60474057041356	23.58382986747937
90-91	23.040874626487966	26.677957193330805	26.743413048344866	23.537755131836366
92-93	23.334428743031363	26.59728558206831	26.751303997847952	23.316981677052375
94-95	22.691272265627944	26.58442791276719	27.116522854701774	23.607776966903092
96-97	22.920355931108396	26.546108753969804	26.9167882007453	23.616747114176498
98-99	22.741385569667315	26.864172651647355	26.747899863056762	23.64654191562856
100	23.126823819160354	26.38922919590029	26.52487888247044	23.959068102468922
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	963.0
1	728.5
2	531.0
3	556.5
4	514.0
5	484.0
6	450.0
7	447.0
8	505.0
9	511.0
10	455.0
11	403.0
12	392.0
13	406.5
14	487.0
15	557.5
16	607.5
17	738.5
18	919.5
19	1212.0
20	1746.5
21	2456.0
22	3451.0
23	5042.5
24	7409.5
25	10194.5
26	14080.5
27	18939.5
28	23807.5
29	30577.5
30	38794.0
31	48436.5
32	61780.0
33	71258.0
34	77067.5
35	85999.0
36	96607.0
37	109602.5
38	115898.5
39	116537.0
40	117526.5
41	117976.5
42	121506.5
43	128910.0
44	138687.5
45	154002.0
46	166185.0
47	170516.5
48	174205.5
49	171364.0
50	164501.0
51	165367.0
52	166062.5
53	162458.0
54	157131.0
55	143460.5
56	129715.0
57	113116.0
58	96724.0
59	86960.0
60	72771.0
61	57696.0
62	46715.0
63	36587.0
64	26557.0
65	18763.5
66	14090.5
67	10538.0
68	7543.0
69	5585.5
70	4346.0
71	3041.5
72	2161.5
73	1791.5
74	1547.0
75	1219.0
76	832.5
77	561.0
78	336.5
79	204.0
80	138.5
81	98.0
82	61.0
83	64.5
84	57.5
85	36.0
86	36.0
87	25.5
88	17.0
89	16.0
90	16.0
91	16.0
92	9.0
93	7.5
94	7.5
95	8.0
96	7.5
97	6.0
98	6.5
99	8.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0623692987370217
2	0.008975924818237523
3	0.011092199775382955
4	0.009486749807893316
5	0.011384099769471981
6	0.011846274760112937
7	0.0032108999349792764
8	0.005813674882273083
9	0.007297499852225628
10-11	0.0070907373564125685
12-13	0.006202874874391784
14-15	0.009900274799519436
16-17	0.009474587308139607
18-19	0.007917787339664806
20-21	0.007029924857644022
22-23	0.0027730499438457386
24-25	0.003977137419462967
26-27	0.00509608739680423
28-29	0.006166387375130656
30-31	0.008793487321931883
32-33	0.010240824792623298
34-35	0.011554374766023912
36-37	0.017355887148543286
38-39	0.017939687136721335
40-41	0.017903199637460208
42-43	0.023473624524659103
44-45	0.027134536950525628
46-47	0.029773799397080563
48-49	0.031123836869742304
50-51	0.03140357436407762
52-53	0.029956236893386206
54-55	0.036998324250783934
56-57	0.03946731170078694
58-59	0.03659696175891153
60-61	0.037740236735760206
62-63	0.032437386843142915
64-65	0.03482123679486995
66-67	0.03698616175103023
68-69	0.039820024193644506
70-71	0.039297036704235004
72-73	0.03603748677024089
74-75	0.03833619922369197
76-77	0.03865242421728841
78-79	0.03729022424487296
80-81	0.042082249147834455
82-83	0.038469986720982764
84-85	0.0346266367988106
86-87	0.033045511830828385
88-89	0.03063733687959393
90-91	0.03300902433156726
92-93	0.0346266367988106
94-95	0.03843349922172164
96-97	0.03360498681949902
98-99	0.03077112437688473
100	0.027876449435501896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4110997.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.92795769632198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.74476294307249	36.38519904894624
2	15.053161850225063	15.934662272092003
3	6.530384206415657	10.369196970536912
4	3.3565049294574747	7.106118036552858
5	1.9099417055208763	5.054465689612499
6	1.173948073427116	3.7280804380817454
7	0.7624930058653725	2.825003829072868
8	0.5439508993655312	2.3032168152396104
9	0.3713608569824859	1.7689834555595003
>10	1.5096119968964674	12.565166268748623
>50	0.03484786690017693	1.226934730490854
>100	0.008947423666643943	0.7058185488939669
>500	8.424220465799977E-5	0.027153896172295577
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.297499852225628E-5
2	0.0	0.0	0.0	0.0	7.297499852225628E-5
3	0.0	0.0	0.0	2.432499950741876E-5	7.297499852225628E-5
4	0.0	0.0	0.0	2.432499950741876E-5	7.297499852225628E-5
5	0.0	0.0	0.0	2.432499950741876E-5	7.297499852225628E-5
6	0.0	0.0	0.0	2.432499950741876E-5	7.297499852225628E-5
7	0.0	0.0	0.0	4.864999901483752E-5	7.297499852225628E-5
8	0.0	0.0	0.0	4.864999901483752E-5	7.297499852225628E-5
9	0.0	0.0	0.0	1.216249975370938E-4	7.297499852225628E-5
10-11	0.0	0.0	0.0	1.5811249679822194E-4	8.513749827596565E-5
12-13	0.0	0.0	0.0	1.9459999605935007E-4	2.1892499556676884E-4
14-15	0.0	0.0	0.0	2.310874953204782E-4	3.162249935964439E-4
16-17	0.0	0.0	0.0	2.432499950741876E-4	3.8919999211870014E-4
18-19	0.0	0.0	0.0	2.675749945816064E-4	3.8919999211870014E-4
20-21	0.0	0.0	0.0	3.8919999211870014E-4	4.5001249088724704E-4
22-23	0.0	0.0	0.0	6.324499871928878E-4	4.6217499064095646E-4
24-25	0.0	0.0	0.0	9.973249798041692E-4	4.6217499064095646E-4
26-27	0.0	0.0	0.0	0.0019216749610860822	4.864999901483752E-4
28-29	0.0	0.0	0.0	0.00509608739680423	4.864999901483752E-4
30-31	0.0	0.0	0.0	0.013865249719228694	4.986624899020846E-4
32-33	0.0	0.0	0.0	0.0338360743148195	5.10824989655794E-4
34-35	0.0	0.0	0.0	0.06571398616929178	5.837999881780502E-4
36-37	0.0	0.0	0.0	0.11258826022008772	5.837999881780502E-4
38-39	0.0	0.0	0.0	0.17711032141351601	6.567749867003065E-4
40-41	0.0	0.0	0.0	0.24604737001754076	6.567749867003065E-4
42-43	0.0	0.0	0.0	0.31615201859792164	6.567749867003065E-4
44-45	0.0	0.0	0.0	0.4031503793362048	6.810999862077252E-4
46-47	0.0	0.0	0.0	0.504962664774506	7.05424985715144E-4
48-49	0.0	0.0	0.0	0.6090006876677361	7.540749847299816E-4
50-51	0.0	0.0	0.0	0.7170766604791976	8.027249837448191E-4
52-53	0.0	0.0	0.0	0.8234255583256325	9.243499812819129E-4
54-55	0.0	0.0	0.0	0.9383368559986787	9.486749807893316E-4
56-57	0.0	0.0	0.0	1.0738027782554938	9.486749807893316E-4
58-59	0.0	0.0	0.0	1.2190716753137987	9.486749807893316E-4
60-61	0.0	0.0	0.0	1.370044784756593	9.486749807893316E-4
62-63	0.0	0.0	0.0	1.5176002317685953	0.001021649979311588
64-65	0.0	0.0	0.0	1.6681354912202564	0.001058137478572716
66-67	0.0	0.0	0.0	1.84032000023352	0.0010702999783264255
68-69	0.0	0.0	0.0	2.026515708963057	0.0010702999783264255
70-71	0.0	0.0	0.0	2.227549667392119	0.0010702999783264255
72-73	0.0	0.0	0.0	2.42746467584384	0.001082462478080135
74-75	0.0	0.0	0.0	2.6291310842600955	0.0011067874775875536
76-77	0.0	0.0	0.0	2.8470101048480454	0.001118949977341263
78-79	0.0	0.0	0.0	3.0866721624948887	0.001118949977341263
80-81	0.0	0.0	0.0	3.3383142823991356	0.0011311124770949723
82-83	0.0	0.0	0.0	3.5987498896253145	0.0011432749768486817
84-85	0.0	0.0	0.0	3.8561327094133127	0.0011432749768486817
86-87	0.0	0.0	0.0	4.129411916379409	0.0012040874756172287
88	0.0	0.0	0.0	4.3435935370422305	0.0012648999743857755
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6495	0.0	36.26551	1
GGTATCA	4020	0.0	34.500957	1
CAACGCA	6285	0.0	34.3935	5
ATCAACG	6490	0.0	33.669556	3
TATCAAC	7835	0.0	29.74896	2
GTACTAG	1515	0.0	29.170938	1
AACGCAG	7755	0.0	28.90311	6
TCAACGC	7885	0.0	27.474075	4
ACGCAGA	7925	0.0	27.33341	7
CGCAGAG	8300	0.0	25.929258	8
GTACATG	14160	0.0	25.366777	1
ACATGGG	13610	0.0	24.825607	3
TACATGG	14395	0.0	24.418505	2
GCTATTG	2450	0.0	22.439981	8
CATGGGG	11250	0.0	21.846054	4
GCAGAGT	9660	0.0	21.840971	9
TAGGCAT	2725	0.0	20.866169	5
CTATTGA	2880	0.0	20.231678	9
GGGCTAT	3220	0.0	19.992756	6
GTGATCG	1330	0.0	19.785137	8
>>END_MODULE