FastQCFastQC Report
Mon 16 Jan 2017
ERR1146571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4110997
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125740.30586254380628347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112170.2728535194747162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61910.15059607195042957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77300.055.761971
GTATCAA143200.044.4279061
CAACGCA176300.035.6034135
ATCAACG177000.035.5157363
TATCAAC187000.033.8704032
AACGCAG193600.032.9688536
TCAACGC193850.032.5983854
ACGCAGA208250.030.240367
CGCAGAG210800.029.829968
GTGGTAT20550.029.3103051
GCAGAGT230200.027.193569
GTACATG138850.024.6382961
TACATGG140700.023.6785982
ACATGGG134550.023.6574333
AGAGTAC217850.023.35272210-11
GAGTACT162550.022.7972212-13
GTACTAG16350.022.7369141
CAGAGTA228950.022.57975610-11
AGTACTT172850.021.47953612-13
GTACTTT178600.020.57749414-15
Produced by FastQC (version 0.11.5)