##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146571_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4110997 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48720176638417 34.0 31.0 34.0 31.0 34.0 2 32.52411300713671 34.0 31.0 34.0 31.0 34.0 3 32.6613232751082 34.0 31.0 34.0 31.0 34.0 4 36.16210325621741 37.0 35.0 37.0 35.0 37.0 5 36.18414097602115 37.0 37.0 37.0 35.0 37.0 6 36.277065393139424 37.0 37.0 37.0 35.0 37.0 7 36.267234931088495 37.0 37.0 37.0 35.0 37.0 8 36.2817581720444 37.0 37.0 37.0 35.0 37.0 9 38.09161865114472 39.0 38.0 39.0 37.0 39.0 10-11 38.06328270246853 39.0 38.0 39.0 36.0 39.0 12-13 38.042996382629326 39.0 38.0 39.0 35.0 39.0 14-15 39.507988329838234 41.0 39.0 41.0 37.0 41.0 16-17 39.50733885235139 41.0 39.0 41.0 37.0 41.0 18-19 39.55240054906389 41.0 39.5 41.0 37.0 41.0 20-21 39.58323090481457 41.0 40.0 41.0 37.0 41.0 22-23 39.54574693194863 41.0 39.5 41.0 37.0 41.0 24-25 39.53091476349898 41.0 39.0 41.0 36.5 41.0 26-27 39.459721936065634 41.0 39.0 41.0 36.0 41.0 28-29 39.37474607741139 41.0 39.0 41.0 36.0 41.0 30-31 39.27673627589609 41.0 39.0 41.0 36.0 41.0 32-33 39.15757649543408 41.0 39.0 41.0 35.0 41.0 34-35 39.07764977692759 41.0 39.0 41.0 35.0 41.0 36-37 38.99289637039385 41.0 39.0 41.0 35.0 41.0 38-39 38.889670559234176 41.0 39.0 41.0 35.0 41.0 40-41 38.79608608325426 40.0 38.0 41.0 35.0 41.0 42-43 38.69600306203094 40.0 38.0 41.0 35.0 41.0 44-45 38.537351401618636 40.0 38.0 41.0 35.0 41.0 46-47 38.300261955919694 40.0 38.0 41.0 34.5 41.0 48-49 38.07717665568717 40.0 38.0 41.0 34.0 41.0 50-51 37.91689485543288 40.0 37.5 41.0 33.5 41.0 52-53 37.775386968173414 40.0 37.0 41.0 33.0 41.0 54-55 37.62698878155348 40.0 37.0 41.0 33.0 41.0 56-57 37.415938031577255 40.0 36.0 41.0 33.0 41.0 58-59 37.19287255621933 39.0 35.5 41.0 33.0 41.0 60-61 36.994020428621084 39.0 35.0 41.0 33.0 41.0 62-63 36.724211547709714 39.0 35.0 41.0 32.0 41.0 64-65 36.42322726092965 38.0 35.0 41.0 32.0 41.0 66-67 36.15715883519253 37.0 35.0 40.0 32.0 41.0 68-69 35.8408842185971 37.0 35.0 40.0 31.0 41.0 70-71 35.55530167013014 36.0 35.0 39.0 31.0 41.0 72-73 35.239446294901214 36.0 35.0 39.0 31.0 41.0 74-75 34.92056732223351 35.0 35.0 38.5 31.0 40.5 76-77 34.62530585646256 35.0 35.0 37.0 31.0 39.5 78-79 34.3533185502203 35.0 35.0 37.0 31.0 39.0 80-81 34.11224479122704 35.0 35.0 36.5 30.5 39.0 82-83 33.87190187684399 35.0 34.0 36.0 30.0 37.0 84-85 33.67620239080691 35.0 34.0 36.0 30.0 37.0 86-87 33.50437193216147 35.0 34.0 35.0 30.0 37.0 88-89 33.3654124534754 35.0 34.0 35.0 30.0 36.0 90-91 33.28871414890354 35.0 34.0 35.0 30.0 36.0 92-93 33.209853351875466 35.0 34.0 35.0 30.0 36.0 94-95 33.144792005442966 35.0 34.0 35.0 30.0 36.0 96-97 33.101570616568196 35.0 34.0 35.0 30.0 35.5 98-99 33.051390088584355 35.0 34.0 35.0 30.0 35.0 100 32.51032754341587 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26290205573822334 1101 2 0.2154301730123933 1101 3 0.24406488579972319 1101 4 0.17473414599295722 1101 5 0.19963130241374216 1101 6 0.16333390177697993 1101 7 0.19197504863780068 1101 8 0.15250916623009658 1101 9 0.21561258980775477 1101 10-11 0.2019829145325076 1101 12-13 0.20930045387731866 1101 14-15 0.32830328202997094 1101 16-17 0.3208179944222991 1101 18-19 0.2978385122957192 1101 20-21 0.3052013788664141 1101 22-23 0.32750391124019274 1101 24-25 0.3177533592490249 1101 26-27 0.29670552834305397 1101 28-29 0.35073401886119626 1101 30-31 0.29607623805582506 1101 32-33 0.28338061361579037 1101 34-35 0.31026905736203503 1101 36-37 0.317784391306148 1101 38-39 0.2985604590757518 1101 40-41 0.3120249045350434 1101 42-43 0.2685060329592446 1101 44-45 0.2579733846650143 1101 46-47 0.235992872096503 1101 48-49 0.23437447089551 1101 50-51 0.205697299517702 1101 52-53 0.21682820514855905 1101 54-55 0.17250726494226853 1101 56-57 0.21128105074703996 1101 58-59 0.1964232474623202 1101 60-61 0.19914627010750507 1101 62-63 0.2241087221333089 1101 64-65 0.2407269575253963 1101 66-67 0.1392472754075058 1101 68-69 0.16864935745856258 1101 70-71 0.20463297980639084 1101 72-73 0.13525147524944003 1101 74-75 0.13612785111003944 1101 76-77 0.18539794512173557 1101 78-79 0.1593893705054512 1101 80-81 0.13754890740477066 1101 82-83 0.19324147717929918 1101 84-85 0.14647932520735196 1101 86-87 0.15472847557877856 1101 88-89 0.1900778849735829 1101 90-91 0.13472540563224555 1101 92-93 0.1300021901468753 1101 94-95 0.1376214105785536 1101 96-97 0.13566537546262225 1101 98-99 0.10276538707380922 1101 100 0.13593231797896976 1102 1 0.19971884963135267 1102 2 0.21436219773067933 1102 3 0.2040125411977627 1102 4 0.1437723139318834 1102 5 0.09887303269363912 1102 6 0.13809362551380389 1102 7 0.15450685525357244 1102 8 0.1541307583784075 1102 9 0.1788088820651197 1102 10-11 0.15411304902905698 1102 12-13 0.2146930383920349 1102 14-15 0.3096237109648996 1102 16-17 0.27843231648700595 1102 18-19 0.23798336871156067 1102 20-21 0.29060832179625606 1102 22-23 0.30712077783415737 1102 24-25 0.27618229418177265 1102 26-27 0.3105470405204471 1102 28-29 0.3404515761131037 1102 30-31 0.33293301740879144 1102 32-33 0.31068032281244484 1102 34-35 0.2968186756826441 1102 36-37 0.3767605817823352 1102 38-39 0.3033754354479825 1102 40-41 0.35043184746489686 1102 42-43 0.31395932267206916 1102 44-45 0.32774892083367746 1102 46-47 0.3991073759132959 1102 48-49 0.36735338765304704 1102 50-51 0.37360278843082284 1102 52-53 0.3681288231056712 1102 54-55 0.41008214680341126 1102 56-57 0.3961396476208989 1102 58-59 0.3705950031875176 1102 60-61 0.33683892732306475 1102 62-63 0.37054710817474756 1102 64-65 0.3639047110695657 1102 66-67 0.3113274280792595 1102 68-69 0.24202340326009164 1102 70-71 0.25867096599322537 1102 72-73 0.27840850905532477 1102 74-75 0.20741647342919833 1102 76-77 0.2825869672954937 1102 78-79 0.2400987489133044 1102 80-81 0.2162824588406096 1102 82-83 0.2349964753617826 1102 84-85 0.233939339747522 1102 86-87 0.2568764217801629 1102 88-89 0.2932676341557041 1102 90-91 0.2571308945453694 1102 92-93 0.2103847782966426 1102 94-95 0.229252272083464 1102 96-97 0.10529314718200311 1102 98-99 0.15720340961107127 1102 100 0.13619404098878363 1103 1 0.1495893054350077 1103 2 0.17517127338160776 1103 3 0.16877072009212668 1103 4 0.10174315726091265 1103 5 0.10067238066682194 1103 6 0.1011905244320701 1103 7 0.11220507755781739 1103 8 0.11406833252229376 1103 9 0.1475874126321699 1103 10-11 0.18206768834925668 1103 12-13 0.18510191720468328 1103 14-15 0.2119787404467104 1103 16-17 0.19956055135725848 1103 18-19 0.2054899021608918 1103 20-21 0.21604608486974541 1103 22-23 0.2632291443400092 1103 24-25 0.27870725485805536 1103 26-27 0.22285539120894526 1103 28-29 0.29676787063238663 1103 30-31 0.3117739386822649 1103 32-33 0.2496640924413498 1103 34-35 0.2843141545532859 1103 36-37 0.2462006120110658 1103 38-39 0.31142951325183077 1103 40-41 0.3311414319076391 1103 42-43 0.33582895245792344 1103 44-45 0.34896914245249633 1103 46-47 0.3419876121192402 1103 48-49 0.2825094962304533 1103 50-51 0.33583539152927244 1103 52-53 0.3508797963128174 1103 54-55 0.2594512625249905 1103 56-57 0.33506526208147847 1103 58-59 0.3109537409620131 1103 60-61 0.3456986712575514 1103 62-63 0.3050087642525767 1103 64-65 0.28159785424305284 1103 66-67 0.2724604784575533 1103 68-69 0.32575877879062887 1103 70-71 0.29281710084502777 1103 72-73 0.2218416675189374 1103 74-75 0.23593377147224004 1103 76-77 0.2624764390834642 1103 78-79 0.23533767016920137 1103 80-81 0.26028105285286074 1103 82-83 0.24342157575809154 1103 84-85 0.1811360637960604 1103 86-87 0.20901030696368395 1103 88-89 0.21120847722110625 1103 90-91 0.18791895067943187 1103 92-93 0.19680486986160162 1103 94-95 0.19287334080989638 1103 96-97 0.1664034908581229 1103 98-99 0.15352441418932017 1103 100 0.22901137681774486 1104 1 0.12916976002050262 1104 2 0.10790273597382338 1104 3 0.09737654384987593 1104 4 0.09663771914431152 1104 5 0.11428514741457008 1104 6 0.1095469500067594 1104 7 0.11656802388382914 1104 8 0.11102105266106577 1104 9 0.10555463613254545 1104 10-11 0.11907182225630208 1104 12-13 0.14649443585212651 1104 14-15 0.2245631033587756 1104 16-17 0.18959734258312722 1104 18-19 0.21244578859562324 1104 20-21 0.1866882592335628 1104 22-23 0.22045026900227782 1104 24-25 0.16331192475588807 1104 26-27 0.18468828259385361 1104 28-29 0.20902065858364693 1104 30-31 0.22444662649398595 1104 32-33 0.22183290437344283 1104 34-35 0.23771549824601834 1104 36-37 0.29053073189878376 1104 38-39 0.23591966284975996 1104 40-41 0.26715512656588913 1104 42-43 0.2974595691826707 1104 44-45 0.27045832733816155 1104 46-47 0.33801205281439906 1104 48-49 0.35823079538158 1104 50-51 0.3469572366721039 1104 52-53 0.36557551731351623 1104 54-55 0.3309339514310139 1104 56-57 0.32498621602334055 1104 58-59 0.3833461634874027 1104 60-61 0.3860419330523115 1104 62-63 0.3805555745388034 1104 64-65 0.4283799495048157 1104 66-67 0.3891639150481936 1104 68-69 0.3407552847309887 1104 70-71 0.3619195336816361 1104 72-73 0.33400089952811385 1104 74-75 0.3403232584209519 1104 76-77 0.3250040972320747 1104 78-79 0.31763952516668326 1104 80-81 0.3135773705218128 1104 82-83 0.2982999214099564 1104 84-85 0.316646366149655 1104 86-87 0.3208507846777664 1104 88-89 0.29855911168923654 1104 90-91 0.24500087295444217 1104 92-93 0.20669102946543916 1104 94-95 0.2485898555995547 1104 96-97 0.22102025144747728 1104 98-99 0.21155620878143822 1104 100 0.2539509645365001 1105 1 0.1654890503061921 1105 2 0.1508894829121985 1105 3 0.14201462151843458 1105 4 0.07763059842842068 1105 5 0.1188900811752589 1105 6 0.0760673931775031 1105 7 0.12199042833118057 1105 8 0.13716061859101103 1105 9 0.09807021600732924 1105 10-11 0.139713931896857 1105 12-13 0.16052808418446318 1105 14-15 0.23684546807477602 1105 16-17 0.250436172691181 1105 18-19 0.21815202690472546 1105 20-21 0.21980983383421204 1105 22-23 0.20730364309533655 1105 24-25 0.22220187963982596 1105 26-27 0.25040228064526815 1105 28-29 0.25100423105893555 1105 30-31 0.2999521715808484 1105 32-33 0.2720412077098189 1105 34-35 0.3111788248568672 1105 36-37 0.2747258759447533 1105 38-39 0.250282049345536 1105 40-41 0.2100332884675069 1105 42-43 0.16525155698721505 1105 44-45 0.23759035980670973 1105 46-47 0.26043431606602496 1105 48-49 0.2610299738975712 1105 50-51 0.1903357716614167 1105 52-53 0.24626957455808451 1105 54-55 0.19082263948423162 1105 56-57 0.21121862369721356 1105 58-59 0.19194041252866612 1105 60-61 0.15765456524579236 1105 62-63 0.16582239548733924 1105 64-65 0.1398502675553459 1105 66-67 0.11644552433214272 1105 68-69 0.1343405760535532 1105 70-71 0.09062083533893883 1105 72-73 0.13587483069319717 1105 74-75 0.0903583512991517 1105 76-77 0.06759054443226375 1105 78-79 0.09434189273449789 1105 80-81 0.08532546059112889 1105 82-83 0.14986681049636275 1105 84-85 0.08860407831209471 1105 86-87 0.04881827428035024 1105 88-89 0.029009826610739253 1105 90-91 0.084749688466772 1105 92-93 0.06553509415021352 1105 94-95 0.047073567463620236 1105 96-97 0.06552522978594766 1105 98-99 0.02507316124108172 1105 100 0.06665957616396412 1106 1 0.087262401285642 1106 2 0.06311587124599782 1106 3 0.09207350386777335 1106 4 0.0684218750392418 1106 5 0.04968284588292704 1106 6 0.06583504861917078 1106 7 0.07211882914509005 1106 8 0.0802798854161253 1106 9 0.06719742262651351 1106 10-11 0.0809213510129112 1106 12-13 0.11014061242503459 1106 14-15 0.14083974903441288 1106 16-17 0.13705753754099703 1106 18-19 0.11759170927938811 1106 20-21 0.10130759191618921 1106 22-23 0.1096809527481426 1106 24-25 0.0984134764588731 1106 26-27 0.06110239243839288 1106 28-29 0.12532463485720058 1106 30-31 0.1401797940397671 1106 32-33 0.09830728014500778 1106 34-35 0.08357559668196757 1106 36-37 0.0796260523459793 1106 38-39 0.08857700452936257 1106 40-41 0.005008227645596719 1106 42-43 -0.04203138502221293 1106 44-45 0.008917854308485573 1106 46-47 -0.037957445153551816 1106 48-49 -0.04446938331894046 1106 50-51 -0.03516004877698009 1106 52-53 -0.02975317425776325 1106 54-55 -0.010065308262340977 1106 56-57 -0.058229054350100284 1106 58-59 -0.019532613287836398 1106 60-61 0.02914849936254882 1106 62-63 0.02493998943259612 1106 64-65 0.07398679846225775 1106 66-67 -0.022403159361665814 1106 68-69 -0.009537704099805921 1106 70-71 -0.037105568523344346 1106 72-73 -0.0206413607237792 1106 74-75 0.04054113180031038 1106 76-77 0.002021487085855256 1106 78-79 -0.030070827627639574 1106 80-81 -0.015418965550878738 1106 82-83 -0.056721411522886456 1106 84-85 -0.009936741407429395 1106 86-87 0.00729894838701739 1106 88-89 0.00421342368015587 1106 90-91 0.011668529991169407 1106 92-93 -0.06037805891116221 1106 94-95 -0.005602362815743334 1106 96-97 -0.05210761163604616 1106 98-99 -0.08052396249915006 1106 100 -0.03628497897842209 1107 1 0.04387799567696504 1107 2 0.016282855756138304 1107 3 0.07576236213535736 1107 4 0.03714252256846606 1107 5 0.04074396913225797 1107 6 0.04988900916944772 1107 7 0.04997830924031632 1107 8 0.02229886909673695 1107 9 0.03081627560963085 1107 10-11 -0.0019025236240750587 1107 12-13 0.03356598495057739 1107 14-15 0.04980762538463068 1107 16-17 0.036184401697610724 1107 18-19 0.039446920847602485 1107 20-21 0.05202205992615205 1107 22-23 0.04030032544165607 1107 24-25 0.09448875660177691 1107 26-27 0.04083243133858616 1107 28-29 0.05587224569098481 1107 30-31 0.05105279282987851 1107 32-33 0.049918464260187534 1107 34-35 0.0815778540750145 1107 36-37 0.10069519512305902 1107 38-39 0.02717511660137717 1107 40-41 0.03665975347445993 1107 42-43 0.096702281476162 1107 44-45 7.800430351068144E-4 1107 46-47 0.04083081113891751 1107 48-49 0.08996030523813658 1107 50-51 0.09724022749184513 1107 52-53 0.023120205005355388 1107 54-55 0.018089447928616664 1107 56-57 -0.015992142310018664 1107 58-59 -0.0488408693662592 1107 60-61 -0.017384172882564997 1107 62-63 0.0010671516118208046 1107 64-65 0.04376372541766216 1107 66-67 -0.022788696779230122 1107 68-69 -0.020254778852155653 1107 70-71 -0.030704880221051667 1107 72-73 -0.007346210105502848 1107 74-75 -0.042229791675588046 1107 76-77 6.664478989932832E-4 1107 78-79 -0.00929969382138296 1107 80-81 -0.0032867747722917784 1107 82-83 0.06334591275925305 1107 84-85 0.022836546373980582 1107 86-87 0.003344756615305755 1107 88-89 0.06189012439391206 1107 90-91 0.08819417866541812 1107 92-93 0.03445954551551722 1107 94-95 0.07372827772103818 1107 96-97 0.03410773094854136 1107 98-99 0.028311886771952288 1107 100 0.10377519669221158 1108 1 0.07227984115124997 1108 2 0.038565720235574474 1108 3 0.03364202586834608 1108 4 0.03226415998537391 1108 5 4.959942070072998E-4 1108 6 -0.01253390220961137 1108 7 0.017651017027873195 1108 8 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-0.34556366635501945 2214 48-49 -0.3504471849256774 2214 50-51 -0.2725505826576935 2214 52-53 -0.3225973017364936 2214 54-55 -0.3110893142724862 2214 56-57 -0.21171510740791888 2214 58-59 -0.24129945730079783 2214 60-61 -0.24488130920946105 2214 62-63 -0.2582747048245366 2214 64-65 -0.10674397812520198 2214 66-67 -0.16690045325442782 2214 68-69 -0.12028113861441625 2214 70-71 -0.12766895574601733 2214 72-73 -0.18195791632816594 2214 74-75 -0.17185580150860602 2214 76-77 -0.13846202694121246 2214 78-79 -0.12415966161814396 2214 80-81 -0.14197297628171412 2214 82-83 -0.1268520538109783 2214 84-85 -0.10608628877641024 2214 86-87 -0.08566225228751989 2214 88-89 -0.08752222444985591 2214 90-91 -0.11960215624911541 2214 92-93 -0.10014454448523935 2214 94-95 -0.13215424631281536 2214 96-97 -0.1098857399149793 2214 98-99 -0.09437963305729369 2214 100 -0.12156990155331471 2215 1 -0.3330724156223397 2215 2 -0.33305389548888087 2215 3 -0.3418617591967674 2215 4 -0.2516470823430055 2215 5 -0.19820339633654527 2215 6 -0.1962247523190257 2215 7 -0.195589483860104 2215 8 -0.22152425584307878 2215 9 -0.30016827180220673 2215 10-11 -0.283742717555036 2215 12-13 -0.2926691049238599 2215 14-15 -0.4108735823647578 2215 16-17 -0.3899159723468273 2215 18-19 -0.38516774606960524 2215 20-21 -0.37731607493739006 2215 22-23 -0.3286186478012212 2215 24-25 -0.37208470279043127 2215 26-27 -0.3961987159709679 2215 28-29 -0.3891348079588326 2215 30-31 -0.43263097928815597 2215 32-33 -0.4140729573050024 2215 34-35 -0.41023720100328376 2215 36-37 -0.3909423267413814 2215 38-39 -0.42794431865790017 2215 40-41 -0.3803351953830756 2215 42-43 -0.352620914046895 2215 44-45 -0.36622850828825904 2215 46-47 -0.35034413339948856 2215 48-49 -0.4045201787291859 2215 50-51 -0.37205204228758504 2215 52-53 -0.3688765902350042 2215 54-55 -0.37598483181847797 2215 56-57 -0.4021174401853216 2215 58-59 -0.40730351961273925 2215 60-61 -0.4055291798427305 2215 62-63 -0.2991747011655548 2215 64-65 -0.3113442108498816 2215 66-67 -0.3059879219528554 2215 68-69 -0.2836760187052363 2215 70-71 -0.273947072358915 2215 72-73 -0.31543037524598816 2215 74-75 -0.2999658552248121 2215 76-77 -0.27536862496067727 2215 78-79 -0.2565620456550093 2215 80-81 -0.26182599147593066 2215 82-83 -0.25892333379502475 2215 84-85 -0.27930591211045197 2215 86-87 -0.26904485540123346 2215 88-89 -0.2517777828550365 2215 90-91 -0.2847949702128716 2215 92-93 -0.3245982476690372 2215 94-95 -0.3193747579192916 2215 96-97 -0.32092980905252233 2215 98-99 -0.32706725742260545 2215 100 -0.26462071265834197 2216 1 -0.31300033434308006 2216 2 -0.34063598224673086 2216 3 -0.30514393977978216 2216 4 -0.24425619780462426 2216 5 -0.2090293064157791 2216 6 -0.19232139815819238 2216 7 -0.23175753375138441 2216 8 -0.28435699597120845 2216 9 -0.30898851606335853 2216 10-11 -0.3500049422746301 2216 12-13 -0.3561393147040448 2216 14-15 -0.42941782183353894 2216 16-17 -0.44532889186753977 2216 18-19 -0.48228121926692324 2216 20-21 -0.4430713659323402 2216 22-23 -0.38481232024224 2216 24-25 -0.4240215132352603 2216 26-27 -0.4401690066948021 2216 28-29 -0.4294397843391451 2216 30-31 -0.45748808080805503 2216 32-33 -0.43583897469395083 2216 34-35 -0.46544333734426857 2216 36-37 -0.47651786996951273 2216 38-39 -0.4819522681048696 2216 40-41 -0.4420638658259506 2216 42-43 -0.44359982220991867 2216 44-45 -0.46376591147260626 2216 46-47 -0.50561623229612 2216 48-49 -0.49526680113770993 2216 50-51 -0.5201161790135913 2216 52-53 -0.4558100686117683 2216 54-55 -0.4990268146132806 2216 56-57 -0.5076372913468958 2216 58-59 -0.4707479979185436 2216 60-61 -0.42145042700782653 2216 62-63 -0.40433514046023333 2216 64-65 -0.35609314703890504 2216 66-67 -0.41735144619872955 2216 68-69 -0.39678377383221175 2216 70-71 -0.3535365985122354 2216 72-73 -0.3989427436000952 2216 74-75 -0.4286198567966366 2216 76-77 -0.41018229948704743 2216 78-79 -0.37371725909245157 2216 80-81 -0.33625879598010755 2216 82-83 -0.29960347665048914 2216 84-85 -0.3142097967658799 2216 86-87 -0.34880291481867687 2216 88-89 -0.3625681424555509 2216 90-91 -0.30681714995447606 2216 92-93 -0.3249519844040165 2216 94-95 -0.3061701545743105 2216 96-97 -0.27928869823897884 2216 98-99 -0.2776697330588007 2216 100 -0.38803707261924814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 25.0 8 108.0 9 141.0 10 210.0 11 234.0 12 203.0 13 222.0 14 350.0 15 706.0 16 1404.0 17 2544.0 18 4777.0 19 7662.0 20 11240.0 21 16763.0 22 24850.0 23 29879.0 24 28807.0 25 24565.0 26 21175.0 27 22106.0 28 26807.0 29 33144.0 30 39506.0 31 50554.0 32 65823.0 33 88337.0 34 130425.0 35 214741.0 36 397454.0 37 999884.0 38 1375170.0 39 489980.0 40 1200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.613434427200566 19.903147028279637 13.382125525479797 24.10129301904 2 15.498677095394228 20.970131667229847 39.28352324017657 24.247667997199358 3 17.645613627109046 26.595932553341267 30.94445560695717 24.813998212592516 4 12.470381272474778 17.91081336230603 38.9340590615853 30.684746303633887 5 12.840648820596082 38.29222721569566 35.2714191295782 13.595704834130052 6 29.546742719582603 38.67795783908912 17.988665040714487 13.786634400613796 7 25.698182556777617 33.51529252650514 23.20326344352158 17.583261473195662 8 25.96311794924686 34.220871482027356 20.7911852039785 19.024825364747286 9 26.62658231081171 14.50677293123785 20.912761551516578 37.953883206433865 10-11 24.449215117403394 26.02131551056836 28.762280780063815 20.76718859196443 12-13 25.665829967766946 24.00590416388044 28.40618711227471 21.922078756077905 14-15 22.85864718589314 24.951839500597607 26.543522873485447 25.6459904400238 16-17 21.73362082239418 27.70122430155021 27.40617422002497 23.158980656030643 18-19 21.66909876120075 26.609944011148635 29.959849155813057 21.761108071837562 20-21 22.807496575648194 25.895226875621656 29.575623626093623 21.72165292263653 22-23 22.7756210868506 25.789319283800772 29.24365461365772 22.19140501569091 24-25 22.426080583371867 25.96816538664465 29.588090188341177 22.017663841642307 26-27 22.314878848123705 26.209396406759723 29.23228842054616 22.243436324570414 28-29 22.01298931122644 26.350892381176287 29.446505110501825 22.189613197095447 30-31 22.31696818749144 25.977701737755332 29.40161902489253 22.303711049860702 32-33 22.501508476598573 26.25485123763192 28.843045579284816 22.400594706484693 34-35 22.59379926252993 26.309792647508306 29.235870620062798 21.860537469898965 36-37 22.6231985377646 25.994231039614533 28.98773670227387 22.394833720346995 38-39 22.599432834034626 25.934123512575873 29.225454552530962 22.24098910085853 40-41 22.55733719554438 25.722534775467633 29.357587940871976 22.362540088116013 42-43 22.64753312876668 25.83778048300807 28.851524331322086 22.663162056903165 44-45 22.526294562589616 26.33569639891865 28.6889711159656 22.449037922526134 46-47 22.919860602598334 26.17413291062801 28.04317607441826 22.862830412355397 48-49 23.17570383041079 26.19417709060808 27.854770979786032 22.775348099195106 50-51 22.98998287504622 26.4828409909121 27.551387023955478 22.975789110086208 52-53 22.77219100546212 26.676044870888134 27.53741500901069 23.01434911463906 54-55 22.786115688326422 26.650242207260526 27.4093139723207 23.154328132092353 56-57 23.118983301933493 26.555520554226455 27.282140038020366 23.043356105819694 58-59 22.71560914920321 26.523260697461517 27.748225537830155 23.01290461550512 60-61 22.877683613963878 26.71512052895203 27.46549982351887 22.94169603356523 62-63 22.962966746891354 26.550114644921834 27.381831565130625 23.105087043056187 64-65 22.461662150130632 26.63114658389324 27.423835147245562 23.48335611873057 66-67 22.740144535780733 26.472761125771623 27.418583074503942 23.368511263943702 68-69 22.634878562709478 26.357579437419936 27.665047535227544 23.342494464643043 70-71 22.65191105621144 26.212914901201877 27.45060215642019 23.684571886166488 72-73 22.882281979993582 26.227728988725012 27.497402561702994 23.392586469578408 74-75 22.907839675590402 26.539251894832034 27.242548838714107 23.310359590863452 76-77 22.87820450134741 26.375771189372145 27.094550578005855 23.651473731274592 78-79 22.60209802397905 26.514818241514426 27.181498935711783 23.701584798794737 80-81 22.67177962312764 26.642485977843798 27.204746589267703 23.48098780976085 82-83 22.912992823988684 26.22155231035066 27.64196494346798 23.22348992219268 84-85 23.047457992818522 26.467981523201146 27.090884478411365 23.39367600556897 86-87 22.51752647260256 26.770133900657395 27.20651238854433 23.50582723819571 88-89 22.81953043178534 26.291748008599875 27.100507067702313 23.78821449191247 90-91 22.630605179230244 26.317812929564287 27.216609498863658 23.834972392341808 92-93 22.846769287352924 26.26288708067654 27.171620412274688 23.718723219695857 94-95 22.229187282108764 26.139808941677394 27.605548631037347 24.025455145176497 96-97 22.44690947290222 26.139830383241343 27.394204803314835 24.019055340541602 98-99 22.258539640839462 26.422588315423955 27.231605640997575 24.08726640273901 100 22.55048269711987 25.969876157970774 27.08625691839114 24.393384226518215 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 372.0 1 244.5 2 286.0 3 439.0 4 290.0 5 245.5 6 530.0 7 1339.5 8 2673.5 9 3258.0 10 2377.5 11 1529.0 12 1535.5 13 1797.5 14 2191.5 15 2658.0 16 3139.0 17 3704.0 18 4402.0 19 5154.0 20 6084.0 21 7257.5 22 8561.5 23 10318.5 24 12611.5 25 15044.5 26 18734.0 27 23308.0 28 27939.5 29 34047.0 30 41448.5 31 50572.5 32 62906.5 33 71229.5 34 76240.0 35 84281.0 36 94435.0 37 107333.0 38 113132.0 39 113792.0 40 115869.0 41 116351.0 42 119357.0 43 126560.5 44 135983.0 45 151169.5 46 162734.5 47 167789.5 48 172589.5 49 168604.0 50 161448.0 51 161582.0 52 161444.0 53 158151.5 54 153413.5 55 140691.0 56 127316.0 57 111619.0 58 95413.0 59 86041.0 60 72114.5 61 56204.0 62 44828.5 63 34832.0 64 25135.5 65 17622.0 66 13110.0 67 9829.5 68 6989.5 69 5096.0 70 3830.5 71 2566.5 72 1821.0 73 1535.0 74 1350.0 75 1140.5 76 756.5 77 406.5 78 188.5 79 104.5 80 85.5 81 40.0 82 11.5 83 6.0 84 4.0 85 3.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005594749886706315 2 0.0819509233404938 3 3.648749926112814E-4 4 0.0 5 0.03972272419561484 6 0.0042568749137982835 7 4.864999901483752E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.513749827596566E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.378499911335377E-4 24-25 0.0 26-27 0.0 28-29 3.648749926112814E-5 30-31 9.729999802967503E-5 32-33 0.0014959874697062538 34-35 0.0012284124751246474 36-37 7.540749847299816E-4 38-39 7.540749847299816E-4 40-41 0.0012892249738931944 42-43 7.419124849762722E-4 44-45 5.716374884243409E-4 46-47 4.0136249187240955E-4 48-49 6.810999862077253E-4 50-51 9.000249817744941E-4 52-53 0.0011311124770949723 54-55 0.0013378749729080319 56-57 0.001021649979311588 58-59 0.0025176374490178417 60-61 0.0018486999625638258 62-63 0.0010459749788190066 64-65 0.00430552491281312 66-67 0.004402824910842796 68-69 0.0036730749256202325 70-71 0.0015446374687210912 72-73 0.005254199893602452 74-75 0.003514962428822011 76-77 0.0033811749315312075 78-79 8.148874834985284E-4 80-81 7.297499852225628E-5 82-83 2.0676249581305948E-4 84-85 8.513749827596566E-5 86-87 3.648749926112814E-5 88-89 1.824374963056407E-4 90-91 0.0 92-93 0.0 94-95 2.432499950741876E-5 96-97 5.716374884243409E-4 98-99 5.351499891632127E-4 100 8.270499832522379E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4110997.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.61854289011565 #Duplication Level Percentage of deduplicated Percentage of total 1 65.29852493649072 31.747191352860604 2 16.846469653092562 16.38101614751825 3 7.112489771302553 10.37396667004746 4 3.6996692301446554 7.194901085801162 5 2.080712813997654 5.058061259467908 6 1.3017547887801029 3.797365261843132 7 0.8772120687633732 2.9854140812229404 8 0.5943303312140402 2.3116379759221166 9 0.4313808264875412 1.8875796493102253 >10 1.6963798427910526 13.078676611144605 >50 0.04180948360894334 1.3772440548187193 >100 0.01725966733665452 1.485570205223734 >500 9.120845410022647E-4 0.3259743713359311 >1k 8.208760869020262E-4 0.6658728281839591 >5k 9.120845410022513E-5 0.2745350745396171 >10k+ 1.8241690820045025E-4 1.0549933707594945 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12574 0.30586254380628347 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11217 0.2728535194747162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6191 0.15059607195042957 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.297499852225628E-5 2 0.0 0.0 0.0 0.0 7.297499852225628E-5 3 0.0 0.0 0.0 2.432499950741876E-5 7.297499852225628E-5 4 0.0 0.0 0.0 4.864999901483752E-5 7.297499852225628E-5 5 0.0 0.0 0.0 4.864999901483752E-5 7.297499852225628E-5 6 0.0 0.0 0.0 4.864999901483752E-5 1.216249975370938E-4 7 0.0 0.0 0.0 9.729999802967503E-5 1.216249975370938E-4 8 0.0 0.0 0.0 9.729999802967503E-5 1.216249975370938E-4 9 0.0 0.0 0.0 1.4594999704451255E-4 1.216249975370938E-4 10-11 0.0 0.0 0.0 1.702749965519313E-4 1.216249975370938E-4 12-13 0.0 0.0 0.0 2.0676249581305946E-4 1.824374963056407E-4 14-15 0.0 0.0 0.0 2.432499950741876E-4 2.1892499556676884E-4 16-17 0.0 0.0 0.0 2.432499950741876E-4 3.040624938427345E-4 18-19 0.0 0.0 0.0 2.675749945816064E-4 3.162249935964439E-4 20-21 0.0 0.0 0.0 3.648749926112814E-4 3.5271249285757206E-4 22-23 0.0 0.0 0.0 5.959624879317597E-4 3.8919999211870014E-4 24-25 0.0 0.0 0.0 9.851624800504598E-4 3.8919999211870014E-4 26-27 0.0 0.0 0.0 0.0019095124613323728 4.256874913798283E-4 28-29 0.0 0.0 0.0 0.005035274898035683 4.378499911335377E-4 30-31 0.0 0.0 0.0 0.013865249719228694 4.5001249088724704E-4 32-33 0.0 0.0 0.0 0.03386039931432691 4.6217499064095646E-4 34-35 0.0 0.0 0.0 0.06575047366855291 4.6217499064095646E-4 36-37 0.0 0.0 0.0 0.11263691021910256 4.6217499064095646E-4 38-39 0.0 0.0 0.0 0.17723194641105308 4.6217499064095646E-4 40-41 0.0 0.0 0.0 0.2463635950111372 4.6217499064095646E-4 42-43 0.0 0.0 0.0 0.3168817685831442 4.6217499064095646E-4 44-45 0.0 0.0 0.0 0.4044639293096054 5.10824989655794E-4 46-47 0.0 0.0 0.0 0.506847852236331 5.716374884243409E-4 48-49 0.0 0.0 0.0 0.6116399501142911 6.567749867003065E-4 50-51 0.0 0.0 0.0 0.7201537729168861 8.027249837448191E-4 52-53 0.0 0.0 0.0 0.8269405207544545 8.270499832522378E-4 54-55 0.0 0.0 0.0 0.9426423809114918 9.000249817744941E-4 56-57 0.0 0.0 0.0 1.0784245281619032 9.365124810356223E-4 58-59 0.0 0.0 0.0 1.2242650627086324 9.486749807893316E-4 60-61 0.0 0.0 0.0 1.3761381971332014 9.486749807893316E-4 62-63 0.0 0.0 0.0 1.5244112316306726 9.486749807893316E-4 64-65 0.0 0.0 0.0 1.6753356910744523 9.729999802967503E-4 66-67 0.0 0.0 0.0 1.8484202250694906 9.729999802967503E-4 68-69 0.0 0.0 0.0 2.035661908777846 9.729999802967503E-4 70-71 0.0 0.0 0.0 2.237437779691885 9.851624800504598E-4 72-73 0.0 0.0 0.0 2.4386176881179917 0.0010338124790652973 74-75 0.0 0.0 0.0 2.641451696510603 0.0010946249778338443 76-77 0.0 0.0 0.0 2.860461829575648 0.001118949977341263 78-79 0.0 0.0 0.0 3.101182024701064 0.001118949977341263 80-81 0.0 0.0 0.0 3.3544539195723084 0.0011311124770949723 82-83 0.0 0.0 0.0 3.616494976765977 0.0011919249758635191 84-85 0.0 0.0 0.0 3.8750089090310693 0.001216249975370938 86-87 0.0 0.0 0.0 4.149504365972536 0.0012648999743857755 88 2.432499950741876E-5 0.0 0.0 4.364950886609744 0.001313549973400613 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7730 0.0 55.76197 1 GTATCAA 14320 0.0 44.427906 1 CAACGCA 17630 0.0 35.603413 5 ATCAACG 17700 0.0 35.515736 3 TATCAAC 18700 0.0 33.870403 2 AACGCAG 19360 0.0 32.968853 6 TCAACGC 19385 0.0 32.598385 4 ACGCAGA 20825 0.0 30.24036 7 CGCAGAG 21080 0.0 29.82996 8 GTGGTAT 2055 0.0 29.310305 1 GCAGAGT 23020 0.0 27.19356 9 GTACATG 13885 0.0 24.638296 1 TACATGG 14070 0.0 23.678598 2 ACATGGG 13455 0.0 23.657433 3 AGAGTAC 21785 0.0 23.352722 10-11 GAGTACT 16255 0.0 22.79722 12-13 GTACTAG 1635 0.0 22.736914 1 CAGAGTA 22895 0.0 22.579756 10-11 AGTACTT 17285 0.0 21.479536 12-13 GTACTTT 17860 0.0 20.577494 14-15 >>END_MODULE