FastQCFastQC Report
Mon 16 Jan 2017
ERR1146570_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146570_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3874228
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37450.043.9452131
GTATCAA64600.040.033681
CAACGCA70500.033.8623855
ATCAACG72000.033.3535923
AACGCAG79100.030.6548636
TATCAAC83700.030.600272
TCAACGC82850.029.1557884
ACGCAGA88800.026.7750387
GTACTAG14900.026.1931151
GTACATG149100.025.7340341
CGCAGAG92600.025.5767698
TACATGG154650.024.5840152
ACATGGG157750.023.2369673
CTAACGC6450.022.5868783
GCAGAGT105000.021.9744669
CATGGGG118150.021.757444
TAGGCAT24100.021.2545765
CTAGTAC22750.020.8638383
TAACGCC7350.020.4605274
TAGTACT25100.020.4083044
Produced by FastQC (version 0.11.5)