FastQCFastQC Report
Mon 16 Jan 2017
ERR1146570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3874228
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134410.34693363426210333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121580.31381735922614773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65670.16950473745995331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72000.060.862161
GTATCAA139500.046.2586981
CAACGCA179950.035.1440475
ATCAACG180450.035.0727273
TATCAAC186400.034.442372
AACGCAG191550.033.3452226
TCAACGC192450.033.0812644
GTGGTAT19150.031.7067051
ACGCAGA214150.029.517027
CGCAGAG220200.028.7060388
GTACTAG14850.026.6245961
GCAGAGT238850.026.2284839
GTACATG150700.024.8928851
TACATGG153950.024.183622
ACATGGG158850.022.8809283
AGAGTAC226950.022.47853310-11
CAGAGTA234950.022.45324910-11
GAGTACT161850.022.35868812-13
TGGTATC26800.022.1289252
AGTACTT166000.021.36089512-13
Produced by FastQC (version 0.11.5)