##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146570_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3874228
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48364525784233	34.0	31.0	34.0	31.0	34.0
2	32.52237787760555	34.0	31.0	34.0	31.0	34.0
3	32.66022650189922	34.0	31.0	34.0	31.0	34.0
4	36.163365966071176	37.0	35.0	37.0	35.0	37.0
5	36.18581972976293	37.0	37.0	37.0	35.0	37.0
6	36.27990892637191	37.0	37.0	37.0	35.0	37.0
7	36.27095230327178	37.0	37.0	37.0	35.0	37.0
8	36.28459734429672	37.0	37.0	37.0	35.0	37.0
9	38.09953776597557	39.0	39.0	39.0	37.0	39.0
10-11	38.072061324217366	39.0	38.0	39.0	36.0	39.0
12-13	38.05427610352308	39.0	38.0	39.0	35.0	39.0
14-15	39.5131999200873	41.0	39.0	41.0	37.0	41.0
16-17	39.51339686254913	41.0	39.0	41.0	37.0	41.0
18-19	39.56420672712086	41.0	39.5	41.0	37.0	41.0
20-21	39.59386257081411	41.0	40.0	41.0	37.0	41.0
22-23	39.55348601063231	41.0	39.5	41.0	37.0	41.0
24-25	39.535056532553064	41.0	39.0	41.0	37.0	41.0
26-27	39.46281736645339	41.0	39.0	41.0	36.0	41.0
28-29	39.377897222362755	41.0	39.0	41.0	36.0	41.0
30-31	39.27692833256071	41.0	39.0	41.0	35.5	41.0
32-33	39.150731448949315	41.0	39.0	41.0	35.0	41.0
34-35	39.070279808003036	41.0	39.0	41.0	35.0	41.0
36-37	38.98560577746069	41.0	39.0	41.0	35.0	41.0
38-39	38.87361598233248	41.0	39.0	41.0	35.0	41.0
40-41	38.77937630413078	40.0	38.0	41.0	35.0	41.0
42-43	38.668278041457555	40.0	38.0	41.0	35.0	41.0
44-45	38.505282471759536	40.0	38.0	41.0	35.0	41.0
46-47	38.251260638248446	40.0	38.0	41.0	34.0	41.0
48-49	38.012274832560195	40.0	38.0	41.0	34.0	41.0
50-51	37.84042059476107	40.0	37.0	41.0	33.0	41.0
52-53	37.691283657027924	40.0	37.0	41.0	33.0	41.0
54-55	37.53490734154005	40.0	36.5	41.0	33.0	41.0
56-57	37.31046636904178	39.5	36.0	41.0	33.0	41.0
58-59	37.07154264023697	39.0	35.0	41.0	33.0	41.0
60-61	36.86111426586148	39.0	35.0	41.0	32.5	41.0
62-63	36.580385563265764	38.5	35.0	41.0	32.0	41.0
64-65	36.25823376941161	37.5	35.0	40.5	32.0	41.0
66-67	35.97951372505697	37.0	35.0	40.0	31.5	41.0
68-69	35.65539457151205	36.5	35.0	39.5	31.0	41.0
70-71	35.37592999172996	36.0	35.0	39.0	31.0	41.0
72-73	35.063839040965064	35.5	35.0	39.0	31.0	41.0
74-75	34.756000034071306	35.0	35.0	37.5	31.0	40.0
76-77	34.477924634275524	35.0	35.0	37.0	31.0	39.0
78-79	34.21777900010015	35.0	35.0	36.5	31.0	39.0
80-81	33.9975593847342	35.0	34.5	36.0	30.5	39.0
82-83	33.77421837331205	35.0	34.0	36.0	30.0	37.0
84-85	33.59831907156729	35.0	34.0	35.5	30.0	37.0
86-87	33.438533044518806	35.0	34.0	35.0	30.0	36.5
88-89	33.31632482652028	35.0	34.0	35.0	30.0	36.0
90-91	33.246214084457606	35.0	34.0	35.0	30.0	36.0
92-93	33.17422813009456	35.0	34.0	35.0	30.0	36.0
94-95	33.1129708938142	35.0	34.0	35.0	30.0	36.0
96-97	33.072775789137864	35.0	34.0	35.0	30.0	35.0
98-99	33.025521729748476	35.0	34.0	35.0	29.0	35.0
100	32.47579621023853	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24202455317870175
1101	2	0.28169167494422
1101	3	0.2518889520388612
1101	4	0.20451763602255824
1101	5	0.15891797228672289
1101	6	0.17879216787638086
1101	7	0.18304097848249512
1101	8	0.18927717494280216
1101	9	0.2784748409309117
1101	10-11	0.2768912292820076
1101	12-13	0.27680057862457375
1101	14-15	0.4030422282233488
1101	16-17	0.40013871854154104
1101	18-19	0.3886218087059916
1101	20-21	0.3705365548353754
1101	22-23	0.3905540788959314
1101	24-25	0.39341704749915607
1101	26-27	0.3860474929910467
1101	28-29	0.42256551398169506
1101	30-31	0.4313968751316608
1101	32-33	0.3790913510170242
1101	34-35	0.37492798173328623
1101	36-37	0.4365607857747591
1101	38-39	0.44166168713027076
1101	40-41	0.428421109204983
1101	42-43	0.4282943109748558
1101	44-45	0.44367145958932497
1101	46-47	0.45426762841002244
1101	48-49	0.39143190603552114
1101	50-51	0.3224593489467509
1101	52-53	0.3683779176517632
1101	54-55	0.32822051299891797
1101	56-57	0.3822450648294051
1101	58-59	0.3706783239995559
1101	60-61	0.32172958760240533
1101	62-63	0.3875315029833857
1101	64-65	0.4213055175419811
1101	66-67	0.3561113807916314
1101	68-69	0.36290332110916523
1101	70-71	0.35733978498198127
1101	72-73	0.2772423330300242
1101	74-75	0.3218846470870673
1101	76-77	0.3499946316303948
1101	78-79	0.31012594322518794
1101	80-81	0.28650992037372447
1101	82-83	0.27712304383727115
1101	84-85	0.2811921729239444
1101	86-87	0.26410547528913497
1101	88-89	0.26494883333563735
1101	90-91	0.2422108451504954
1101	92-93	0.21503518054405646
1101	94-95	0.21334335624547407
1101	96-97	0.21202719832588457
1101	98-99	0.26329480227667545
1101	100	0.2812836831410408
1102	1	0.22174787827454168
1102	2	0.24434508062141447
1102	3	0.22319291279130482
1102	4	0.16295706691255418
1102	5	0.11459624025872017
1102	6	0.11332318036357947
1102	7	0.164583418637136
1102	8	0.16468315907012254
1102	9	0.17550327498706508
1102	10-11	0.18999363872518416
1102	12-13	0.23669709492098434
1102	14-15	0.34397263374616216
1102	16-17	0.2928651124186601
1102	18-19	0.30384623923617227
1102	20-21	0.27618623971161327
1102	22-23	0.32313756034675833
1102	24-25	0.2890280026812775
1102	26-27	0.28307012724669534
1102	28-29	0.369218472005862
1102	30-31	0.3888570936513531
1102	32-33	0.3486339466979018
1102	34-35	0.323839446939175
1102	36-37	0.3419711907236973
1102	38-39	0.3850099608005735
1102	40-41	0.3701790546836534
1102	42-43	0.34540272275367556
1102	44-45	0.3989660631267853
1102	46-47	0.4093984638964656
1102	48-49	0.4432022798761679
1102	50-51	0.3973586594496794
1102	52-53	0.39460495893161607
1102	54-55	0.3838640279558092
1102	56-57	0.3736361576636824
1102	58-59	0.33316977406338566
1102	60-61	0.36241894654816065
1102	62-63	0.39644093139092007
1102	64-65	0.38254199761558283
1102	66-67	0.33168519664597795
1102	68-69	0.3644263029331256
1102	70-71	0.2913441984775389
1102	72-73	0.33218683847836417
1102	74-75	0.3085306064309208
1102	76-77	0.33147281610052914
1102	78-79	0.29295038730858636
1102	80-81	0.2742156978678949
1102	82-83	0.281045845745318
1102	84-85	0.3218927308265904
1102	86-87	0.2984851091675722
1102	88-89	0.3018271319244832
1102	90-91	0.2314728770083363
1102	92-93	0.2938656301682485
1102	94-95	0.2556332121120448
1102	96-97	0.23969882873146275
1102	98-99	0.2629649004665353
1102	100	0.254045110865718
1103	1	0.21822352917295262
1103	2	0.24259372379447086
1103	3	0.22010876627128795
1103	4	0.1675961603603966
1103	5	0.13253725272850403
1103	6	0.15265803829785796
1103	7	0.13961711113940112
1103	8	0.15294394960555735
1103	9	0.1449868410775892
1103	10-11	0.23009766948978694
1103	12-13	0.22824854866551902
1103	14-15	0.2817301791565825
1103	16-17	0.2991101094615587
1103	18-19	0.26378380771159726
1103	20-21	0.24161817432413812
1103	22-23	0.2643778171898745
1103	24-25	0.2696975179511796
1103	26-27	0.2866682981867186
1103	28-29	0.32261804260178906
1103	30-31	0.31462348999662026
1103	32-33	0.33586371015673677
1103	34-35	0.32068378431227984
1103	36-37	0.3087272685359892
1103	38-39	0.31708687539358493
1103	40-41	0.3150694574988009
1103	42-43	0.290382203664322
1103	44-45	0.29429057754933297
1103	46-47	0.3636665843525009
1103	48-49	0.34156991801157943
1103	50-51	0.3568282028494991
1103	52-53	0.39264124422899727
1103	54-55	0.3612277774749657
1103	56-57	0.36126781013152254
1103	58-59	0.36554114978964236
1103	60-61	0.3287275779389347
1103	62-63	0.3209943015826511
1103	64-65	0.26189069378204266
1103	66-67	0.24924370746619928
1103	68-69	0.26889665526043416
1103	70-71	0.26741685361580636
1103	72-73	0.2619139628353011
1103	74-75	0.21191768764762742
1103	76-77	0.1801048832157619
1103	78-79	0.23033315527013798
1103	80-81	0.19656202182086702
1103	82-83	0.2702770802608896
1103	84-85	0.24195670719130646
1103	86-87	0.2742263730389496
1103	88-89	0.28871582004393304
1103	90-91	0.2655370439801956
1103	92-93	0.18325659423688023
1103	94-95	0.2639998664686658
1103	96-97	0.24946611893198423
1103	98-99	0.2266409436467356
1103	100	0.3208905357755327
1104	1	0.12018523477368603
1104	2	0.1443005433583906
1104	3	0.1531623705870402
1104	4	0.11215480191577143
1104	5	0.0327254346255188
1104	6	0.05750343216803344
1104	7	0.06897906569773227
1104	8	0.08367176486362382
1104	9	0.08525844825704354
1104	10-11	0.1383749112634831
1104	12-13	0.12533809036591492
1104	14-15	0.1697967477892135
1104	16-17	0.17663250818882403
1104	18-19	0.17336560055611727
1104	20-21	0.17952759330585621
1104	22-23	0.15720573210831645
1104	24-25	0.1869276777962483
1104	26-27	0.17633970619272787
1104	28-29	0.24353981538168057
1104	30-31	0.21464331896667943
1104	32-33	0.15568846403908765
1104	34-35	0.14081088856276835
1104	36-37	0.14538624725750537
1104	38-39	0.13377852312576266
1104	40-41	0.1894663907653822
1104	42-43	0.16682047140403
1104	44-45	0.11912204511220637
1104	46-47	0.18695925387500267
1104	48-49	0.1499526933567168
1104	50-51	0.09152635971267387
1104	52-53	0.07153620536919192
1104	54-55	0.09774938948737599
1104	56-57	0.06284311777974239
1104	58-59	0.10454186175461899
1104	60-61	0.12346102558188932
1104	62-63	0.06572321084016863
1104	64-65	0.12878395445456192
1104	66-67	0.09439506400157427
1104	68-69	0.047861192703216204
1104	70-71	0.067232446035284
1104	72-73	0.0486015066887191
1104	74-75	0.00821486223112089
1104	76-77	0.046525488995399655
1104	78-79	0.05206813675849986
1104	80-81	0.09221883298003064
1104	82-83	0.10358516556485853
1104	84-85	0.09747988545301212
1104	86-87	0.07231290504413579
1104	88-89	0.06876463576467984
1104	90-91	0.03358820971927656
1104	92-93	0.06212342291071593
1104	94-95	0.09326495917784428
1104	96-97	0.06763453703493383
1104	98-99	0.032854591685648415
1104	100	0.029871807248866844
1105	1	0.11868038144656623
1105	2	0.09177697592339484
1105	3	0.11262968360760794
1105	4	0.10584132109553224
1105	5	0.07129844613793068
1105	6	0.05863934268785442
1105	7	0.07967177734808217
1105	8	0.07862033090090392
1105	9	0.11576160477532227
1105	10-11	0.11741732970750718
1105	12-13	0.11357277558278867
1105	14-15	0.1816317092415929
1105	16-17	0.14252084769603357
1105	18-19	0.1401790139168284
1105	20-21	0.1357826331206482
1105	22-23	0.1892930940469526
1105	24-25	0.1449657987878865
1105	26-27	0.1395562689224974
1105	28-29	0.18166852352003104
1105	30-31	0.17530433078857754
1105	32-33	0.10593106204711944
1105	34-35	0.14414401821889555
1105	36-37	0.11241329955483081
1105	38-39	0.13278911666438376
1105	40-41	0.14065832618685903
1105	42-43	0.10613717128101996
1105	44-45	0.06630005297688513
1105	46-47	0.06996342638713315
1105	48-49	0.0882886328014294
1105	50-51	0.09085135430975555
1105	52-53	0.06472065681520434
1105	54-55	0.007530843843795765
1105	56-57	0.043828534765083305
1105	58-59	0.017104671909550007
1105	60-61	-0.015013246911152578
1105	62-63	-0.01704772051282788
1105	64-65	-0.010645242905503949
1105	66-67	-0.049470075988232054
1105	68-69	0.00883930533179722
1105	70-71	0.026552349385660534
1105	72-73	7.662058198931732E-4
1105	74-75	0.013805152268830057
1105	76-77	-6.012304879163821E-4
1105	78-79	0.056400207429838645
1105	80-81	0.10556057859211876
1105	82-83	0.09311289348273277
1105	84-85	0.03687311806591964
1105	86-87	0.056039010734572514
1105	88-89	0.08290842872911952
1105	90-91	0.059855871369599356
1105	92-93	0.03360916065348363
1105	94-95	0.059852516386868615
1105	96-97	0.02258964808206798
1105	98-99	-0.033021195701046224
1105	100	8.748710188299924E-4
1106	1	0.12390722758217976
1106	2	0.11806203722220943
1106	3	0.14344083275765485
1106	4	0.11229157115477051
1106	5	0.05438787817295321
1106	6	0.0744863014436703
1106	7	0.06525907359033312
1106	8	0.06328432548814789
1106	9	0.14364461321871858
1106	10-11	0.11871069253660949
1106	12-13	0.1314401631184836
1106	14-15	0.16578962227585237
1106	16-17	0.1548094509811051
1106	18-19	0.15072994711795928
1106	20-21	0.14549561842888892
1106	22-23	0.13925708177471563
1106	24-25	0.1591240002321186
1106	26-27	0.18631902287916802
1106	28-29	0.22455567103627772
1106	30-31	0.20210989413899938
1106	32-33	0.1614823304274111
1106	34-35	0.14855396259474674
1106	36-37	0.16018276021259226
1106	38-39	0.18209981683025944
1106	40-41	0.14419836372692885
1106	42-43	0.11101714596330226
1106	44-45	0.08495744999113697
1106	46-47	0.06828187932485719
1106	48-49	0.06921323619874187
1106	50-51	0.12516122822003695
1106	52-53	0.21043278001148025
1106	54-55	0.20214833427169765
1106	56-57	0.25588430545889196
1106	58-59	0.18564093673538196
1106	60-61	0.18078711414695192
1106	62-63	0.17434561924347491
1106	64-65	0.21547070881621977
1106	66-67	0.1921564861025402
1106	68-69	0.2358690099999805
1106	70-71	0.2145714873360518
1106	72-73	0.19061499325082565
1106	74-75	0.1954558862332405
1106	76-77	0.17848830599828602
1106	78-79	0.12935136637023703
1106	80-81	0.12037491272364065
1106	82-83	0.08982209672290509
1106	84-85	0.1299480828331383
1106	86-87	0.08906507052429191
1106	88-89	0.0898723603338567
1106	90-91	0.0678771762922068
1106	92-93	0.07049309356776234
1106	94-95	0.04509898219548347
1106	96-97	0.05495002647595726
1106	98-99	0.16363815938306914
1106	100	0.13730876745314902
1107	1	0.04202178032423376
1107	2	0.0491479633341001
1107	3	0.12614739143161557
1107	4	0.06577664212168344
1107	5	0.05489138759531187
1107	6	0.05074381607396106
1107	7	0.04475598016077953
1107	8	0.1132462171412385
1107	9	0.058343585254270636
1107	10-11	0.06273439026017513
1107	12-13	0.06480541356074809
1107	14-15	0.09054932561216589
1107	16-17	0.13172513434781763
1107	18-19	0.11300459943554841
1107	20-21	0.11389156841050152
1107	22-23	0.1275213408404383
1107	24-25	0.13334673835029776
1107	26-27	0.15352379813602113
1107	28-29	0.18790582288581703
1107	30-31	0.18333812749781941
1107	32-33	0.1582685427228938
1107	34-35	0.1553966185458293
1107	36-37	0.18710652624656632
1107	38-39	0.19173558471891994
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2216	24-25	-0.49255238341174135
2216	26-27	-0.4743367090187647
2216	28-29	-0.4487228094760738
2216	30-31	-0.48691896371721555
2216	32-33	-0.5285548595689491
2216	34-35	-0.5203952242516152
2216	36-37	-0.4993456009815418
2216	38-39	-0.47690456960564376
2216	40-41	-0.45967429539882687
2216	42-43	-0.4699236707102301
2216	44-45	-0.4808829007447599
2216	46-47	-0.5143494341002253
2216	48-49	-0.45616043489463465
2216	50-51	-0.4652152356653261
2216	52-53	-0.426510414077498
2216	54-55	-0.41686636573247426
2216	56-57	-0.456595522305264
2216	58-59	-0.37348627288882597
2216	60-61	-0.39002694183714937
2216	62-63	-0.4144563102812171
2216	64-65	-0.35594711598287887
2216	66-67	-0.37062512353001864
2216	68-69	-0.3572903648322239
2216	70-71	-0.32904864224353503
2216	72-73	-0.30129364702860784
2216	74-75	-0.32521599463945705
2216	76-77	-0.32593196780719325
2216	78-79	-0.3115310253932293
2216	80-81	-0.3249552519653207
2216	82-83	-0.26295591099102467
2216	84-85	-0.31552554336676053
2216	86-87	-0.36022729788263064
2216	88-89	-0.2957622485111955
2216	90-91	-0.2916489290419051
2216	92-93	-0.30406459007788555
2216	94-95	-0.24476420828411705
2216	96-97	-0.25147593060528095
2216	98-99	-0.28923878923253454
2216	100	-0.44256042249548244
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	23.0
8	110.0
9	107.0
10	211.0
11	256.0
12	194.0
13	214.0
14	365.0
15	609.0
16	1195.0
17	2442.0
18	4496.0
19	7244.0
20	10746.0
21	15831.0
22	23401.0
23	28809.0
24	28470.0
25	24203.0
26	20618.0
27	20776.0
28	25436.0
29	31468.0
30	37560.0
31	48276.0
32	62422.0
33	83646.0
34	124894.0
35	206159.0
36	395658.0
37	989111.0
38	1257485.0
39	420667.0
40	1124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2585727085301	19.289604128436462	13.436597591797025	24.015225571236414
2	15.768296425497356	20.82543287616595	38.884069681773894	24.522201016562803
3	17.993749438595515	26.041192376669116	30.514385893182922	25.45067229155245
4	12.774751511785057	17.56104700084765	37.92218733641902	31.74201415094827
5	13.406920798882243	37.89433430051584	34.509075050074095	14.189669850527828
6	30.468229108184815	37.494905222292346	17.870453903070803	14.166411766452033
7	26.669242664407633	33.15747889837121	22.428190191230403	17.745088245990747
8	26.440467623485247	33.669572363836096	20.571582261033683	19.31837775164497
9	27.020479951102516	14.429481176636996	20.332050669191386	38.217988203069105
10-11	25.036768615579675	25.744251499911723	28.0193757311134	21.199604153395203
12-13	26.144253771331993	23.62687482512645	28.01310351378391	22.21576788975765
14-15	23.418716409419584	24.48713626928321	26.26238118271882	25.831766138578388
16-17	22.315981403262793	27.35172013624392	26.907425169607986	23.424873290885305
18-19	22.13866865863341	26.087571511021036	29.52526283946118	22.248496990884377
20-21	23.28800731397326	25.36377569931351	29.211303516468313	22.13691347024491
22-23	23.460611373910307	25.080942339577234	28.951913661848604	22.50653262466385
24-25	22.93014246967396	25.38252782231712	29.140915299770693	22.546414408238235
26-27	22.748183638133842	25.671540239758734	28.86288571555417	22.717390406553253
28-29	22.68605100914983	25.67931947189975	29.050521439569938	22.584108079380492
30-31	22.99207828355798	25.343994738558578	28.8647776773849	22.799149300498538
32-33	23.090529042636202	25.549714272734477	28.53788018994623	22.821876494683092
34-35	23.152874848774022	25.58120205907383	28.77715195181212	22.48877114034003
36-37	23.211970486772092	25.50502749603466	28.463848618874373	22.819153398318875
38-39	23.112699225155563	25.41853189189915	28.80167167544655	22.667097207498742
40-41	23.085938422578188	25.14649936689559	28.79382865488253	22.973733555643687
42-43	23.14369215341738	25.321662974508946	28.28846711032787	23.246177761745805
44-45	23.25231076231442	25.72702269379668	28.039688622850317	22.98097792103858
46-47	23.452271329629788	25.74545698583004	27.625862512881632	23.176409171658534
48-49	23.832417617873304	25.63717637851614	27.42595765527972	23.10444834833083
50-51	23.540508551377247	26.00896623703394	27.03737196874555	23.413153242843265
52-53	23.377750887510047	26.262798993800367	26.964302008049955	23.395148110639628
54-55	23.334678567884033	26.030404709964287	26.948962868041782	23.685953854109894
56-57	23.555782355593724	25.904040353198898	26.973205502774643	23.566971788432735
58-59	23.311299458288552	25.98817199457785	27.32431096118709	23.376217585946506
60-61	23.318479349184866	26.156477396243883	27.089273872999986	23.435769381571266
62-63	23.470461977223852	25.944363258900033	26.942186155563835	23.64298860831228
64-65	23.18143820532733	25.898896075895895	26.978581938999913	23.941083779776868
66-67	23.378995810963822	25.901090631063195	27.032970607052093	23.68694295092089
68-69	23.099188475992555	25.811801493468494	27.321080238890943	23.767929791648008
70-71	23.21167777702636	25.63439525760668	27.052689071856058	24.101237893510906
72-73	23.45870346309423	25.870103668384044	27.016195385922508	23.654997482599217
74-75	23.530851954088735	26.106894484938625	26.737779427487595	23.624474133485045
76-77	23.483053925277286	25.923954133145834	26.627759394932944	23.965232546643943
78-79	23.253909583351188	26.042241516824898	26.722382284023688	23.981466615800226
80-81	23.25518096900362	26.101877871379248	26.81129680836977	23.83164435124736
82-83	23.514495680811354	25.817512311500913	27.08580533438576	23.582186673301976
84-85	23.671211661687174	26.00299414721654	26.540572630655163	23.78522156044112
86-87	23.109126026946793	26.154856181633136	26.82104068760039	23.91497710381968
88-89	23.398965030513878	25.837391031305522	26.687820169244368	24.075823768936228
90-91	23.348612936564393	25.794364709562785	26.843864119509746	24.01315823436308
92-93	23.374605211670556	25.914298280844594	26.63338347665651	24.077713030828335
94-95	23.002866635623924	25.940367990732604	26.950182074983715	24.10658329865976
96-97	23.144285597948798	25.65398078656182	26.831612748439003	24.370120867050375
98-99	23.040501779393878	26.005697932160405	26.59077167295289	24.363028615492826
100	23.141273847832803	25.73383556188002	26.489527254537006	24.635363335750178
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	242.0
1	195.0
2	390.0
3	568.0
4	337.5
5	267.5
6	645.0
7	1663.5
8	3259.0
9	4031.0
10	2832.0
11	1656.5
12	1717.5
13	1948.0
14	2322.5
15	2871.0
16	3404.5
17	4008.0
18	4660.0
19	5271.5
20	5993.0
21	7031.0
22	8385.5
23	9821.0
24	11458.5
25	13870.0
26	16988.0
27	20418.0
28	24230.0
29	30090.0
30	38084.5
31	45398.5
32	54307.0
33	62274.5
34	66782.5
35	72000.0
36	78946.0
37	88365.5
38	93553.5
39	94465.0
40	96000.0
41	96756.5
42	98487.0
43	102658.0
44	110929.0
45	124266.5
46	134639.0
47	144397.5
48	152018.0
49	151290.5
50	148706.5
51	150468.5
52	153142.5
53	155143.5
54	158973.0
55	151942.5
56	139181.0
57	125947.0
58	115685.5
59	106023.5
60	88313.0
61	70648.0
62	55816.0
63	44756.0
64	33139.5
65	21255.5
66	14685.5
67	10831.0
68	8180.5
69	6675.0
70	5209.0
71	3961.0
72	2841.0
73	2244.5
74	1764.5
75	1096.5
76	712.0
77	368.5
78	182.5
79	127.5
80	71.5
81	31.0
82	13.0
83	9.0
84	5.0
85	2.0
86	1.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005652739074726629
2	0.08115165137415764
3	4.1298550317637474E-4
4	0.0
5	0.03786560832248386
6	0.004207289813609318
7	2.581159394852342E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.162318789704684E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.033260819962068E-4
24-25	0.0
26-27	0.0
28-29	3.871739092278513E-5
30-31	1.161521727683554E-4
32-33	0.00203911592193335
34-35	0.001522884042962882
36-37	8.517826003012729E-4
38-39	8.001594124042262E-4
40-41	0.0016648478096797609
42-43	6.581956456873472E-4
44-45	5.033260819962068E-4
46-47	2.710217364594959E-4
48-49	6.71101442661609E-4
50-51	7.098188335843941E-4
52-53	0.0012002391186063392
54-55	0.00150997824598862
56-57	9.550289760953667E-4
58-59	0.003032862288951502
60-61	0.001832623170345163
62-63	0.0011228043367607688
64-65	0.004762239083502571
66-67	0.004813862271399618
68-69	0.004104043437815224
70-71	0.0017035652006025458
72-73	0.004968731835090758
74-75	0.0037684927164844196
76-77	0.003742681122535896
78-79	7.743478184557027E-4
80-81	2.581159394852342E-5
82-83	1.2905796974261712E-4
84-85	7.743478184557026E-5
86-87	2.581159394852342E-5
88-89	1.9358695461392568E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.226449243565428E-4
98-99	4.000797062021131E-4
100	7.485362245071793E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3874228.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.81721807279534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.46944589239037	33.113228811990815
2	16.58049445789204	16.519882163271646
3	6.901537831816373	10.314462456157303
4	3.421147640933625	6.817282323504789
5	1.9863315675521525	4.9476756432811495
6	1.22848749322934	3.6719897609944625
7	0.7906181822998659	2.757047887996463
8	0.5755556700693142	2.2938065863101498
9	0.4103446123594095	1.8398004335014837
>10	1.5726020184287686	12.57417680993279
>50	0.04453071244871711	1.451571302940246
>100	0.017105602177274942	1.4891830697748611
>500	8.991592014369613E-4	0.33859489814228066
>1k	6.294114410058721E-4	0.4304665922935988
>5k	8.991592014369603E-5	0.2941596370022959
>10k+	1.7983184028739206E-4	1.1466716229057061
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13441	0.34693363426210333	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12158	0.31381735922614773	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6567	0.16950473745995331	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.581159394852342E-5
2	0.0	0.0	0.0	0.0	2.581159394852342E-5
3	0.0	0.0	0.0	0.0	2.581159394852342E-5
4	0.0	0.0	0.0	0.0	2.581159394852342E-5
5	0.0	0.0	0.0	0.0	2.581159394852342E-5
6	0.0	0.0	0.0	0.0	1.2905796974261712E-4
7	0.0	0.0	0.0	0.0	1.2905796974261712E-4
8	0.0	0.0	0.0	0.0	1.2905796974261712E-4
9	0.0	0.0	0.0	0.0	1.2905796974261712E-4
10-11	2.581159394852342E-5	0.0	0.0	0.0	1.2905796974261712E-4
12-13	2.581159394852342E-5	0.0	0.0	0.0	5.033260819962067E-4
14-15	7.743478184557026E-5	0.0	0.0	0.0	6.323840517388239E-4
16-17	9.034057881983197E-5	0.0	0.0	0.0	7.356304275329176E-4
18-19	1.0324637579409369E-4	0.0	0.0	2.581159394852342E-5	8.259710063527495E-4
20-21	1.0324637579409369E-4	0.0	0.0	5.162318789704684E-5	9.8084057004389E-4
22-23	1.0324637579409369E-4	0.0	0.0	1.161521727683554E-4	9.8084057004389E-4
24-25	1.2905796974261712E-4	0.0	0.0	4.517028940991599E-4	0.001032463757940937
26-27	1.2905796974261712E-4	0.0	0.0	0.001380920276246003	0.0010582753518894603
28-29	1.2905796974261712E-4	0.0	0.0	0.005020355022987806	0.0010582753518894603
30-31	1.2905796974261712E-4	0.0	0.0	0.013292970883489564	0.0010840869458379836
32-33	1.2905796974261712E-4	0.0	0.0	0.031167499692842035	0.001161521727683554
34-35	1.4196376671687884E-4	0.0	0.0	0.060786303748772655	0.0012389565095291242
36-37	1.5486956369114053E-4	0.0	0.0	0.10855065835051525	0.001251862306503386
38-39	1.8068115763966396E-4	0.0	0.0	0.17141479541214405	0.0013292970883489563
40-41	2.0649275158818737E-4	0.0	0.0	0.23620189622293786	0.001342202885323218
42-43	2.5811593948523424E-4	0.0	0.0	0.30282162020407677	0.001342202885323218
44-45	3.0973912738228106E-4	0.0	0.0	0.386218880251756	0.0013680144792717415
46-47	3.871739092278513E-4	0.0	0.0	0.4822251039432888	0.0014196376671687882
48-49	3.871739092278513E-4	0.0	0.0	0.5825805812151479	0.0014970724490143586
50-51	3.871739092278513E-4	0.0	0.0	0.6881112830736859	0.001600318824808452
52-53	3.871739092278513E-4	0.0	0.0	0.7875891661512953	0.0017293767945510692
54-55	4.387970971248982E-4	0.0	0.0	0.8945136940830534	0.0017293767945510692
56-57	4.387970971248982E-4	0.0	0.0	1.0224875768798325	0.001793905779422378
58-59	4.387970971248982E-4	0.0	0.0	1.1639996407026123	0.0018068115763966396
60-61	4.387970971248982E-4	0.0	0.0	1.305072907428267	0.001832623170345163
62-63	4.387970971248982E-4	0.0	0.0	1.445268579959672	0.0019874927340363035
64-65	4.387970971248982E-4	0.0	0.0	1.5939046437122442	0.002052021718907612
66-67	4.646086910734216E-4	0.0	0.0	1.7619381203171316	0.002103644906804659
68-69	4.646086910734216E-4	0.0	0.0	1.9429419228811522	0.0021165507037789206
70-71	4.7751448804768333E-4	0.0	0.0	2.1330572181090015	0.0021681738916759672
72-73	5.033260819962068E-4	0.0	0.0	2.328463890096298	0.002310137658392846
74-75	5.162318789704685E-4	0.0	0.0	2.526554967854241	0.0024391956281354633
76-77	5.162318789704685E-4	0.0	0.0	2.7360547701374314	0.002503724613006772
78-79	5.162318789704685E-4	0.0	0.0	2.967636391043583	0.0025295362069552955
80-81	5.678550668675152E-4	0.0	0.0	3.2146404393339783	0.002555347800903819
82-83	5.678550668675152E-4	0.0	0.0	3.4674262846688424	0.002671499973672174
84-85	5.936666608160387E-4	0.0	0.0	3.7214381807162615	0.002697311567620698
86-87	6.452898487130855E-4	0.0	0.0	3.979308910058985	0.002826369537363315
88	6.71101442661609E-4	0.0	0.0	4.184549799340669	0.00294252171013167
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7200	0.0	60.86216	1
GTATCAA	13950	0.0	46.258698	1
CAACGCA	17995	0.0	35.144047	5
ATCAACG	18045	0.0	35.072727	3
TATCAAC	18640	0.0	34.44237	2
AACGCAG	19155	0.0	33.345222	6
TCAACGC	19245	0.0	33.081264	4
GTGGTAT	1915	0.0	31.706705	1
ACGCAGA	21415	0.0	29.51702	7
CGCAGAG	22020	0.0	28.706038	8
GTACTAG	1485	0.0	26.624596	1
GCAGAGT	23885	0.0	26.228483	9
GTACATG	15070	0.0	24.892885	1
TACATGG	15395	0.0	24.18362	2
ACATGGG	15885	0.0	22.880928	3
AGAGTAC	22695	0.0	22.478533	10-11
CAGAGTA	23495	0.0	22.453249	10-11
GAGTACT	16185	0.0	22.358688	12-13
TGGTATC	2680	0.0	22.128925	2
AGTACTT	16600	0.0	21.360895	12-13
>>END_MODULE