FastQCFastQC Report
Mon 16 Jan 2017
ERR1146569_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146569_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125322
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.041.1496961
GTATCAA51400.036.9554751
CAACGCA53650.032.6705475
ATCAACG55350.031.922833
TATCAAC64800.028.9353712
AACGCAG63800.028.797816
GTACTAG12750.028.3949471
GTACATG114950.027.1593671
TACATGG115150.026.7305892
ACGCAGA66700.026.4885657
ACATGGG116850.025.3765053
CGCAGAG69600.025.2514658
TGATCGC13800.024.8581059
GGCGTAA950.001306742824.731466
TCAACGC71850.024.656924
GTGATCG14600.023.8178798
TAACGCC10300.023.7241334
CTAACGC10050.023.3794943
CATGGGG96000.023.2023164
GTATAAG21950.022.9197461
Produced by FastQC (version 0.11.5)