##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146569_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3125322
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214889217814996	31.0	31.0	34.0	28.0	34.0
2	31.41266947853693	31.0	31.0	34.0	28.0	34.0
3	31.50142385328616	31.0	31.0	34.0	28.0	34.0
4	35.096399347011285	37.0	35.0	37.0	32.0	37.0
5	35.11660654486162	37.0	35.0	37.0	32.0	37.0
6	35.30811737158603	37.0	35.0	37.0	32.0	37.0
7	35.27199533360083	37.0	35.0	37.0	33.0	37.0
8	35.18836267111037	37.0	35.0	37.0	32.0	37.0
9	36.84285075265844	39.0	37.0	39.0	33.0	39.0
10-11	36.80323387478154	39.0	37.5	39.0	33.0	39.0
12-13	36.75382456591673	39.0	37.0	39.0	33.0	39.0
14-15	38.16547110985684	40.5	38.5	41.0	33.0	41.0
16-17	38.181776789719585	40.5	38.0	41.0	33.0	41.0
18-19	38.20987933403342	40.0	38.5	41.0	33.0	41.0
20-21	38.306422986175505	40.5	39.0	41.0	33.0	41.0
22-23	38.33309095830765	40.0	39.0	41.0	33.0	41.0
24-25	38.38819472041601	41.0	39.0	41.0	33.0	41.0
26-27	38.42274284057771	40.5	38.5	41.0	33.0	41.0
28-29	38.39603327273157	40.0	38.0	41.0	33.0	41.0
30-31	38.387458156311574	40.0	38.0	41.0	33.0	41.0
32-33	38.404145076891275	40.0	38.0	41.0	33.0	41.0
34-35	38.34903107583794	40.0	38.0	41.0	33.0	41.0
36-37	38.26036005250019	40.0	38.0	41.0	33.0	41.0
38-39	38.217155064342165	40.0	38.0	41.0	33.0	41.0
40-41	38.14838151076913	40.0	38.0	41.0	33.0	41.0
42-43	38.01863232012573	40.0	38.0	41.0	32.5	41.0
44-45	37.87509143057899	40.0	37.5	41.0	32.0	41.0
46-47	37.7609007967819	40.0	37.0	41.0	32.0	41.0
48-49	37.62911149635141	40.0	37.0	41.0	32.0	41.0
50-51	37.484015087085425	40.0	36.5	41.0	31.0	41.0
52-53	37.34672523343195	40.0	36.0	41.0	31.0	41.0
54-55	37.154716538007925	39.5	35.5	41.0	31.0	41.0
56-57	36.998259699320585	39.0	35.0	41.0	31.0	41.0
58-59	36.750381400700476	39.0	35.0	41.0	31.0	41.0
60-61	36.556018867815865	38.5	35.0	41.0	31.0	41.0
62-63	36.29423496202951	38.0	35.0	40.0	30.0	41.0
64-65	36.03230099170581	37.0	35.0	40.0	30.0	41.0
66-67	35.76149465559069	37.0	35.0	40.0	30.0	41.0
68-69	35.46627755476075	36.0	35.0	39.0	30.0	41.0
70-71	35.21951338134119	36.0	34.5	39.0	30.0	41.0
72-73	34.91822890569355	35.0	34.0	39.0	29.5	40.5
74-75	34.61009217610217	35.0	34.0	37.5	29.0	40.0
76-77	34.32585762363046	35.0	34.0	37.0	29.0	39.0
78-79	34.067122683678676	35.0	34.0	36.5	29.0	39.0
80-81	33.82135584749347	35.0	34.0	36.0	29.0	39.0
82-83	33.588594391233926	35.0	34.0	36.0	29.0	37.0
84-85	33.388165603416226	35.0	34.0	35.5	28.5	37.0
86-87	33.21022729817919	35.0	34.0	35.0	28.0	36.5
88-89	33.08847200384473	35.0	34.0	35.0	28.0	36.0
90-91	32.9376790615495	35.0	33.5	35.0	27.5	36.0
92-93	32.850068088984116	35.0	33.0	35.0	28.0	36.0
94-95	32.77082249445017	35.0	33.0	35.0	27.0	36.0
96-97	32.70106360240641	35.0	33.0	35.0	27.0	35.0
98-99	32.637200902818975	35.0	33.0	35.0	27.0	35.0
100	31.7275416741059	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17824905244652456
1101	2	0.131652677277863
1101	3	0.16560856459030404
1101	4	0.26025761706159045
1101	5	0.1715852520443235
1101	6	0.3005098037874916
1101	7	0.30388835386459334
1101	8	0.43009540718554007
1101	9	0.3810375676954152
1101	10-11	0.4980429172854599
1101	12-13	0.4774734853626015
1101	14-15	0.6137407272044371
1101	16-17	0.5804043020078922
1101	18-19	0.4998046053786851
1101	20-21	0.46582338926616984
1101	22-23	0.4700183881550757
1101	24-25	0.4305010909402682
1101	26-27	0.4228200157192532
1101	28-29	0.45947473840151787
1101	30-31	0.47451697853666985
1101	32-33	0.4680049428644466
1101	34-35	0.453075100663348
1101	36-37	0.44016088852373514
1101	38-39	0.4350936678007358
1101	40-41	0.4040001895243108
1101	42-43	0.40439870620055984
1101	44-45	0.3208786294698527
1101	46-47	0.33526167647205796
1101	48-49	0.32444793324689414
1101	50-51	0.3201150342757231
1101	52-53	0.30041135695551446
1101	54-55	0.36768705322709394
1101	56-57	0.322824175969771
1101	58-59	0.33650572755549035
1101	60-61	0.3096849046290089
1101	62-63	0.284760784339511
1101	64-65	0.2836423724481705
1101	66-67	0.2536435120682228
1101	68-69	0.2570268945584999
1101	70-71	0.264252682615016
1101	72-73	0.27351698595020935
1101	74-75	0.32085556863250275
1101	76-77	0.27383877418699143
1101	78-79	0.3267706137885682
1101	80-81	0.31076425040292577
1101	82-83	0.29514634232871373
1101	84-85	0.2434300900633133
1101	86-87	0.2565813305199143
1101	88-89	0.2773479525956688
1101	90-91	0.2970686322710989
1101	92-93	0.20628990702584815
1101	94-95	0.23006467053358648
1101	96-97	0.229948352709755
1101	98-99	0.19146843332188723
1101	100	0.10711698451173746
1102	1	0.11744406021154674
1102	2	0.18361106681691552
1102	3	0.19563213459468543
1102	4	0.20655647422500323
1102	5	0.18056805998277525
1102	6	0.20658726486401235
1102	7	0.15111763000231804
1102	8	0.21526562824351458
1102	9	0.37792584342006563
1102	10-11	0.3741282727312054
1102	12-13	0.3961840428451282
1102	14-15	0.436098107862108
1102	16-17	0.37047944591077453
1102	18-19	0.3320006790820855
1102	20-21	0.41670632892182624
1102	22-23	0.33850151777614457
1102	24-25	0.3815516701314081
1102	26-27	0.3871296097542327
1102	28-29	0.3962181721236533
1102	30-31	0.43109758683265653
1102	32-33	0.364785313234421
1102	34-35	0.44288049321178846
1102	36-37	0.48661361429505945
1102	38-39	0.3934849214508702
1102	40-41	0.3277946798778615
1102	42-43	0.2909353173154585
1102	44-45	0.2808841318247417
1102	46-47	0.28536558711468274
1102	48-49	0.24333253744612904
1102	50-51	0.25701624107583143
1102	52-53	0.1880061155563908
1102	54-55	0.22729828231456395
1102	56-57	0.16996853439555082
1102	58-59	0.2701241112685224
1102	60-61	0.30086005025250984
1102	62-63	0.27644546631530886
1102	64-65	0.18473928789275362
1102	66-67	0.2180565234229519
1102	68-69	0.20396995920292227
1102	70-71	0.2493684396243836
1102	72-73	0.22313840275666763
1102	74-75	0.16591431436269488
1102	76-77	0.15749146188822039
1102	78-79	0.17432653584693014
1102	80-81	0.19590812681615688
1102	82-83	0.14493596418710553
1102	84-85	0.14860666536057465
1102	86-87	0.13207075794893086
1102	88-89	0.12778782814834955
1102	90-91	0.16281156388679818
1102	92-93	0.19174383084982338
1102	94-95	0.2097401290561436
1102	96-97	0.2565476302684857
1102	98-99	0.2551484150135934
1102	100	0.08865762463065607
1103	1	0.13858563559951875
1103	2	0.14972561602210988
1103	3	0.26233940961778046
1103	4	0.30347017906472473
1103	5	0.24275927728434255
1103	6	0.31050392882745825
1103	7	0.3382877321599693
1103	8	0.2554927106532503
1103	9	0.42616172270512465
1103	10-11	0.4905649855688594
1103	12-13	0.4663642334160514
1103	14-15	0.5081403657828503
1103	16-17	0.4562929826703481
1103	18-19	0.4434304596648033
1103	20-21	0.4562631932358485
1103	22-23	0.4928771883246341
1103	24-25	0.4777134595902979
1103	26-27	0.5038327398415419
1103	28-29	0.4684472525125756
1103	30-31	0.4735292044028583
1103	32-33	0.44897490620172675
1103	34-35	0.4565355636696822
1103	36-37	0.47250049839664854
1103	38-39	0.4119048659207465
1103	40-41	0.4695182643222182
1103	42-43	0.4771968976052676
1103	44-45	0.39004029498384085
1103	46-47	0.3533264348922174
1103	48-49	0.3024400726991061
1103	50-51	0.3470996924497243
1103	52-53	0.3526289684496149
1103	54-55	0.36487023299343235
1103	56-57	0.3280170085185432
1103	58-59	0.3740173473340249
1103	60-61	0.31593169757012873
1103	62-63	0.27438627422937856
1103	64-65	0.23038827670863782
1103	66-67	0.25990610790815794
1103	68-69	0.19545433880215057
1103	70-71	0.1655957430973558
1103	72-73	0.2622353894484206
1103	74-75	0.3064851561210631
1103	76-77	0.2905627031222835
1103	78-79	0.2867108230561328
1103	80-81	0.28763047979408185
1103	82-83	0.2968922837724648
1103	84-85	0.3271542197883832
1103	86-87	0.3687809824843029
1103	88-89	0.29901428461271706
1103	90-91	0.37304357816940836
1103	92-93	0.2771895195759555
1103	94-95	0.16982848226714964
1103	96-97	0.1761590034917191
1103	98-99	0.23186231592680429
1103	100	0.11528355078351993
1104	1	0.1696792917641794
1104	2	0.17717040236357562
1104	3	0.17711923451849287
1104	4	0.24193713682785756
1104	5	0.2604702786631563
1104	6	0.3060140039480075
1104	7	0.30236293169346595
1104	8	0.35095057194218526
1104	9	0.4114429978609806
1104	10-11	0.44471247134799086
1104	12-13	0.44971557151891517
1104	14-15	0.5653471232632512
1104	16-17	0.5444677114081742
1104	18-19	0.43505739967872614
1104	20-21	0.41454683921329405
1104	22-23	0.44795633380665834
1104	24-25	0.4752326346698368
1104	26-27	0.4704711796759895
1104	28-29	0.480028762427402
1104	30-31	0.5004855678111255
1104	32-33	0.4264180082260367
1104	34-35	0.4050587369696572
1104	36-37	0.38493897726078785
1104	38-39	0.39408243825054967
1104	40-41	0.3956701920522363
1104	42-43	0.33871298051084153
1104	44-45	0.3422632451408205
1104	46-47	0.3020049428892406
1104	48-49	0.3819481185078857
1104	50-51	0.3539374973546856
1104	52-53	0.28660019334312636
1104	54-55	0.3836784508348785
1104	56-57	0.33922332226849505
1104	58-59	0.30077591963711114
1104	60-61	0.26752819790757343
1104	62-63	0.33679643914145174
1104	64-65	0.27387841996851847
1104	66-67	0.33978818828376944
1104	68-69	0.26818222400838465
1104	70-71	0.2713683626054717
1104	72-73	0.23461540508353096
1104	74-75	0.2727243256713976
1104	76-77	0.2675317569890794
1104	78-79	0.2876875527627192
1104	80-81	0.29728472433137654
1104	82-83	0.2603002904353886
1104	84-85	0.31425659026149333
1104	86-87	0.26181363035863825
1104	88-89	0.2127529691958685
1104	90-91	0.23230822272621765
1104	92-93	0.2203736107939278
1104	94-95	0.19184343668023018
1104	96-97	0.16970138962317094
1104	98-99	0.18694032885073852
1104	100	0.23581032387579626
1105	1	0.0014405289363814688
1105	2	-0.03278441228112072
1105	3	-0.06633508964578994
1105	4	0.03668507321606995
1105	5	0.020756060082490535
1105	6	0.04864270289708372
1105	7	0.09908436292157319
1105	8	0.11886717530136082
1105	9	0.21972392533454155
1105	10-11	0.13607787134461802
1105	12-13	0.12863575439234864
1105	14-15	0.1436722900632148
1105	16-17	0.10453794476302392
1105	18-19	0.08804011941516876
1105	20-21	0.0734524855193186
1105	22-23	0.14728353313536502
1105	24-25	0.0896184639002584
1105	26-27	0.11823922039918955
1105	28-29	0.156257010000715
1105	30-31	0.1737815239782421
1105	32-33	0.1390945671059356
1105	34-35	0.14584634265334984
1105	36-37	0.15715380077968177
1105	38-39	0.12551773608560524
1105	40-41	0.1603645105806919
1105	42-43	0.09483042166301914
1105	44-45	0.11099079487184582
1105	46-47	0.13032785344746856
1105	48-49	0.0625829123887911
1105	50-51	0.03148676119874949
1105	52-53	0.03427836132154738
1105	54-55	0.15868034342354775
1105	56-57	0.06100333880435471
1105	58-59	0.07229234343130742
1105	60-61	0.049265370270745734
1105	62-63	0.07781354442682442
1105	64-65	0.09464424787599057
1105	66-67	0.11352087852980475
1105	68-69	0.04422854417365585
1105	70-71	0.019605706920806654
1105	72-73	0.04647534247998664
1105	74-75	0.04192884490769444
1105	76-77	0.010135371838735807
1105	78-79	0.046851796225752196
1105	80-81	0.04647218531830788
1105	82-83	0.10089800550486672
1105	84-85	0.08609586897763677
1105	86-87	0.06495478604494309
1105	88-89	0.04003869869509913
1105	90-91	0.03213777295265885
1105	92-93	0.09818447657812612
1105	94-95	0.08919006453317024
1105	96-97	0.052043903683795634
1105	98-99	-0.019414214110597072
1105	100	4.4262911824688445E-5
1106	1	0.12401759184041339
1106	2	0.10269474826146663
1106	3	0.11925263355335147
1106	4	0.10453419599664215
1106	5	0.105695975011173
1106	6	0.18788113121332373
1106	7	0.20635210642125656
1106	8	0.1838209589147084
1106	9	0.22361368939102988
1106	10-11	0.28112516808170795
1106	12-13	0.28149383403176387
1106	14-15	0.2836901032831918
1106	16-17	0.3177172150993641
1106	18-19	0.2791822256965233
1106	20-21	0.27487510604748167
1106	22-23	0.27023954779798487
1106	24-25	0.22323007540624928
1106	26-27	0.2237392844664825
1106	28-29	0.2985914211720413
1106	30-31	0.22502875086941998
1106	32-33	0.21066122598402615
1106	34-35	0.2202777642689
1106	36-37	0.2299278643294116
1106	38-39	0.21104576364208327
1106	40-41	0.22122080136078637
1106	42-43	0.19062341144491057
1106	44-45	0.12845787558909905
1106	46-47	0.07174374062873312
1106	48-49	0.13625318943586961
1106	50-51	0.13919144363853064
1106	52-53	0.10274881122469282
1106	54-55	0.14833646536747835
1106	56-57	0.11509749008859416
1106	58-59	0.08028545431294276
1106	60-61	0.09722770372718514
1106	62-63	0.14728284111868817
1106	64-65	0.14093045531834747
1106	66-67	0.14135068062468292
1106	68-69	0.15015486929883792
1106	70-71	0.09572383193494005
1106	72-73	0.09447900562169309
1106	74-75	0.09857237197549296
1106	76-77	0.11979197114680318
1106	78-79	0.08879185436368431
1106	80-81	0.03817293327254845
1106	82-83	0.12115178735950138
1106	84-85	0.09953623237737474
1106	86-87	0.1063334136984011
1106	88-89	0.08994141690704538
1106	90-91	0.05914057935134309
1106	92-93	0.02459200988683108
1106	94-95	0.02172645098708159
1106	96-97	0.09718014089860816
1106	98-99	0.05627217272017759
1106	100	0.11101841238710364
1107	1	0.041591021219762325
1107	2	0.09319317295168617
1107	3	0.09860949879373138
1107	4	0.13374563889492208
1107	5	0.11301671378041789
1107	6	0.10114312647908008
1107	7	0.07457738402953851
1107	8	0.0999916384466033
1107	9	0.11207273489813474
1107	10-11	0.13171922725257446
1107	12-13	0.1401334406672916
1107	14-15	0.2216547128271671
1107	16-17	0.21381633496773844
1107	18-19	0.17723613049415832
1107	20-21	0.13265219793303373
1107	22-23	0.16945690255964507
1107	24-25	0.10576187244651436
1107	26-27	0.18691314785733226
1107	28-29	0.21543678914398612
1107	30-31	0.1797044412397284
1107	32-33	0.15230954371463667
1107	34-35	0.11114984721542953
1107	36-37	0.14623266480995767
1107	38-39	0.1860552249977303
1107	40-41	0.13308280090018343
1107	42-43	0.11515600623059896
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2216	60-61	-0.4606133585416359
2216	62-63	-0.5069493217011853
2216	64-65	-0.46575705389274447
2216	66-67	-0.5516371840898913
2216	68-69	-0.47511506531584047
2216	70-71	-0.4960572800109162
2216	72-73	-0.4459637741350946
2216	74-75	-0.4762917530869615
2216	76-77	-0.46352202936362374
2216	78-79	-0.454869094532242
2216	80-81	-0.4239249506011049
2216	82-83	-0.4098831267481202
2216	84-85	-0.4301082641484726
2216	86-87	-0.44443498064086384
2216	88-89	-0.4083349484024339
2216	90-91	-0.3993706195472768
2216	92-93	-0.37462832678106395
2216	94-95	-0.4039893187731991
2216	96-97	-0.34838645399637613
2216	98-99	-0.39177834543781387
2216	100	-0.3686377665077103
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	88.0
1	46.0
2	39.0
3	41.0
4	28.0
5	51.0
6	107.0
7	521.0
8	1553.0
9	1957.0
10	2032.0
11	2785.0
12	3635.0
13	4534.0
14	5366.0
15	6092.0
16	6700.0
17	7387.0
18	8412.0
19	9376.0
20	10427.0
21	11562.0
22	13236.0
23	15503.0
24	19760.0
25	25399.0
26	28758.0
27	27499.0
28	29321.0
29	36786.0
30	41975.0
31	45569.0
32	57264.0
33	77466.0
34	119299.0
35	187621.0
36	364272.0
37	795801.0
38	883505.0
39	273167.0
40	382.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.31072156261086	20.03718398098475	13.227943502376267	24.424150954028125
2	15.288504861427555	20.658635113894107	39.378011192291346	24.67484883238699
3	17.639971621827897	26.296754142563515	30.384868558950153	25.67840567665844
4	12.471960017945587	17.84891657669339	39.1640709349946	30.51505247036642
5	13.549054156023777	38.4258995549986	34.91520140905376	13.109844879923866
6	29.878950849763307	38.190947698331705	18.494052184865637	13.436049267039346
7	26.520104606734417	32.96897603343627	23.076644943458543	17.43427441637077
8	26.024320143762885	34.430999383708794	20.854833662171306	18.689846810357018
9	26.632124650769274	15.304425514458336	20.7182977220125	37.345152112759884
10-11	24.78871089647634	26.282998122941265	28.430512028087403	20.49777895249499
12-13	25.30314401974834	24.260433227678842	27.741271664943802	22.695151087629018
14-15	22.856394971069584	25.34657216635464	25.273436131249344	26.523596731326432
16-17	22.61610040122924	28.094254711527583	26.067804653503984	23.22184023373919
18-19	22.531449564699564	28.090533915419208	27.277339513862515	22.10067700601872
20-21	23.786727601211098	27.18436070246667	26.470192012932696	22.558719683389533
22-23	24.05867259516946	26.87130368505981	26.158510655658233	22.911513064112494
24-25	23.423115695911836	27.157398129499004	26.605050451882285	22.814435722706875
26-27	23.316539580246832	27.370751031143907	26.144737726267692	23.167971662341568
28-29	23.068810157689946	27.47265704996928	26.387914704075367	23.07061808826541
30-31	23.335641037497286	27.230295809060973	26.360402804508553	23.07366034893319
32-33	23.51345215102736	27.50099320524412	25.842952450788943	23.14260219293958
34-35	23.617933906592157	27.268930837414214	26.417874769475645	22.695260486517984
36-37	23.496745574959625	27.08311764910134	26.26570707918453	23.1544296967545
38-39	23.443196246712713	27.17169737201084	26.481685202949695	22.90342117832675
40-41	23.357386020425714	26.71147576396417	26.66780809588005	23.263330119730057
42-43	23.218092277657092	26.986329976836572	26.392178042350267	23.403399703156065
44-45	23.171117533974613	27.331942465638114	26.392890731857044	23.10404926853023
46-47	23.241653982693038	27.011350682758124	26.30086678843068	23.44612854611816
48-49	23.596778535175797	26.742322182970675	26.457377717134722	23.203521564718802
50-51	23.398579132269496	26.936393187823914	26.18870283405371	23.47632484585288
52-53	23.12604231897037	27.12766698027922	26.202592640661948	23.543698060088463
54-55	23.205252382732677	27.00339677044336	26.2157090070112	23.57564183981276
56-57	23.5380459050817	26.798135756252687	26.183293828053607	23.480524510612007
58-59	23.208855936902985	26.88088871774854	26.60290835098218	23.30734699436629
60-61	23.35405702916116	27.127870527421955	26.204634839687706	23.313437603729177
62-63	23.577271683386666	26.704179639769833	26.093649600984552	23.624899075858945
64-65	22.91645561192751	26.935497990758883	26.287794002252422	23.86025239506118
66-67	23.433720940654084	26.724339366811307	26.227688145540956	23.614251546993653
68-69	23.055230255571352	26.69148389448981	26.42212110314226	23.831164746796578
70-71	23.153948597640973	26.60411304018403	26.301508031508565	23.940430330666434
72-73	23.356857947595437	26.665668002859633	26.52066970010798	23.456804349436947
74-75	23.195780013063022	27.050380023209993	26.348094506376345	23.405745457350637
76-77	23.319908671326566	26.77064537926271	26.259458320969774	23.64998762844095
78-79	23.056774653238683	26.876006482459236	26.265755221545373	23.801463642756705
80-81	23.116526385404175	26.87846395058583	26.5393593135896	23.465650350420393
82-83	23.391545934349935	26.407779918309128	27.050158053797666	23.150516093543267
84-85	23.53800498077037	26.83139175258557	26.236170328199194	23.39443293844487
86-87	22.8974084242416	27.045738067364805	26.54101014401022	23.515843364383375
88-89	23.2702296907722	26.468674413818512	26.496728419620077	23.764367475789218
90-91	23.302084303564015	26.52541068164367	26.545671498855565	23.62683351593675
92-93	23.484937356013255	26.481974199815795	26.603461203299084	23.42962724087187
94-95	22.709782492835494	26.60891314917891	26.96764264434885	23.713661713636746
96-97	23.00507547273082	26.60670223583904	26.55942675356991	23.828795537860238
98-99	22.850026789846616	26.8207493603404	26.521772111545623	23.80745173826736
100	23.26798523618645	26.439862296584682	26.26872521921037	24.023427248018496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1276.0
1	968.5
2	712.5
3	760.0
4	660.5
5	536.5
6	501.5
7	490.0
8	510.5
9	535.5
10	472.0
11	412.5
12	396.5
13	408.5
14	472.0
15	602.5
16	680.5
17	728.0
18	861.0
19	1162.0
20	1555.5
21	2110.0
22	3291.5
23	4740.5
24	6390.0
25	8839.5
26	11533.5
27	14478.0
28	18005.5
29	22869.5
30	29617.5
31	36522.0
32	44708.5
33	51597.5
34	56806.0
35	63328.0
36	70731.5
37	80229.0
38	84507.5
39	84258.5
40	84187.5
41	83683.5
42	85340.0
43	90552.0
44	97549.0
45	109913.5
46	121193.5
47	128077.0
48	133505.0
49	131484.0
50	127473.0
51	127309.0
52	125704.5
53	124427.0
54	122140.0
55	111236.0
56	102215.5
57	91690.5
58	79834.0
59	72683.0
60	61324.5
61	48204.0
62	37603.0
63	29559.5
64	22733.5
65	16481.5
66	12053.5
67	8926.0
68	6976.0
69	5345.5
70	4211.5
71	3272.5
72	2415.0
73	1923.0
74	1507.5
75	1170.0
76	834.0
77	550.5
78	383.0
79	294.5
80	207.5
81	140.5
82	98.0
83	68.5
84	44.0
85	29.5
86	19.0
87	16.5
88	17.5
89	11.0
90	8.5
91	8.5
92	7.5
93	9.0
94	7.0
95	2.5
96	4.5
97	9.0
98	9.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06188162371749215
2	0.010046964760751053
3	0.01231873067799094
4	0.010238944979109352
5	0.01241472078717009
6	0.012574704302468674
7	0.0029756933845536556
8	0.005631419738510144
9	0.007647212031272299
10-11	0.007039274673137681
12-13	0.00635134555735377
14-15	0.01035093343981836
16-17	0.009615009269444877
18-19	0.007647212031272299
20-21	0.007039274673137681
22-23	0.002591732947837055
24-25	0.003727615906456999
26-27	0.004863498865076942
28-29	0.006463334018062778
30-31	0.009039068614369976
32-33	0.010830883985714112
34-35	0.012190743865752073
36-37	0.018446099313926694
38-39	0.019022039969001595
40-41	0.018702072938404427
42-43	0.02510141355034777
44-45	0.028589054183856894
46-47	0.031116793725574517
48-49	0.03292460744844851
50-51	0.03308459096374709
52-53	0.03130877394393282
54-55	0.0376921162043463
56-57	0.04053982277666109
58-59	0.037772107961995594
60-61	0.03842804037471979
62-63	0.03422047392236704
64-65	0.036236266215129195
66-67	0.03833205026554064
68-69	0.0410357716740867
70-71	0.0410357716740867
72-73	0.03671621676102495
74-75	0.03956392333333973
76-77	0.03906797443591412
78-79	0.03836404696860036
80-81	0.04351551616121475
82-83	0.039275953005802285
84-85	0.03540435193557656
86-87	0.034060490407068454
88-89	0.03170873273217927
90-91	0.03407648875859831
92-93	0.03582030907535287
94-95	0.03970790849710846
96-97	0.034588436007553784
98-99	0.032124689871955596
100	0.029628947033297687
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3125322.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.510767876046486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.2682974710203	37.21357316735679
2	15.704002357549859	17.12074454474574
3	6.664284839793424	10.898258518855048
4	3.363222487113319	7.333273612421354
5	1.8938231599329285	5.161687733469236
6	1.1527605386376027	3.77027172830044
7	0.7419389440096971	2.8310563088578147
8	0.5310275121521321	2.315737396057546
9	0.35390247760238364	1.7362346226607146
>10	1.3084928165946919	10.804825673585286
>50	0.015198239250532585	0.549201878601512
>100	0.003049156343078276	0.26513481508850856
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.1996703059716724E-5	0.0	0.0	0.0
6	0.0	3.1996703059716724E-5	0.0	0.0	0.0
7	0.0	3.1996703059716724E-5	0.0	0.0	0.0
8	0.0	3.1996703059716724E-5	0.0	0.0	0.0
9	0.0	3.1996703059716724E-5	0.0	0.0	0.0
10-11	0.0	3.1996703059716724E-5	0.0	0.0	0.0
12-13	0.0	3.1996703059716724E-5	0.0	0.0	0.0
14-15	0.0	4.7995054589575086E-5	0.0	3.1996703059716724E-5	0.0
16-17	0.0	6.399340611943345E-5	0.0	3.1996703059716724E-5	0.0
18-19	0.0	6.399340611943345E-5	0.0	4.7995054589575086E-5	0.0
20-21	0.0	6.399340611943345E-5	0.0	1.5998351529858363E-4	0.0
22-23	0.0	6.399340611943345E-5	0.0	2.559736244777338E-4	0.0
24-25	0.0	6.399340611943345E-5	0.0	6.559324127241928E-4	0.0
26-27	0.0	7.999175764929182E-5	0.0	0.001327863176978244	0.0
28-29	3.1996703059716724E-5	1.279868122388669E-4	0.0	0.004287558210002041	0.0
30-31	3.1996703059716724E-5	1.279868122388669E-4	0.0	0.01105486090713213	0.0
32-33	3.1996703059716724E-5	1.279868122388669E-4	0.0	0.02561336079930324	0.0
34-35	3.1996703059716724E-5	1.4398516376872526E-4	0.0	0.04783507107427651	0.0
36-37	3.1996703059716724E-5	2.3997527294787545E-4	0.0	0.08474326805365975	0.0
38-39	3.1996703059716724E-5	3.1996703059716726E-4	0.0	0.13608197811297523	0.0
40-41	3.1996703059716724E-5	3.1996703059716726E-4	0.0	0.1880126271788955	0.0
42-43	3.1996703059716724E-5	3.1996703059716726E-4	0.0	0.24179908502227931	0.0
44-45	3.1996703059716724E-5	3.3596538212702563E-4	0.0	0.30740832464622847	0.0
46-47	3.1996703059716724E-5	3.51963733656884E-4	0.0	0.3892878877760435	0.0
48-49	3.1996703059716724E-5	3.51963733656884E-4	0.0	0.47559899427962943	0.0
50-51	3.1996703059716724E-5	3.51963733656884E-4	0.0	0.5636699194514998	0.0
52-53	3.1996703059716724E-5	3.51963733656884E-4	0.0	0.6452135171991877	0.0
54-55	3.1996703059716724E-5	4.959488974256093E-4	0.0	0.7359401687250146	0.0
56-57	3.1996703059716724E-5	5.439439520151843E-4	0.0	0.8417532657434978	0.0
58-59	3.1996703059716724E-5	6.079373581346178E-4	0.0	0.9597571066277331	0.0
60-61	3.1996703059716724E-5	6.559324127241928E-4	0.0	1.0782888931124537	0.0
62-63	3.1996703059716724E-5	6.719307642540513E-4	0.0	1.195364829607957	0.0
64-65	3.1996703059716724E-5	6.719307642540513E-4	0.0	1.318824108363874	0.0
66-67	3.1996703059716724E-5	6.719307642540513E-4	0.0	1.4577697914006942	0.0
68-69	3.1996703059716724E-5	7.03927467313768E-4	0.0	1.6102340814802443	0.0
70-71	3.1996703059716724E-5	7.199258188436263E-4	0.0	1.772697341265956	0.0
72-73	3.1996703059716724E-5	7.359241703734847E-4	0.0	1.9360405103858098	0.0
74-75	3.1996703059716724E-5	7.359241703734847E-4	0.0	2.1002315921367463	0.0
76-77	3.1996703059716724E-5	7.359241703734847E-4	0.0	2.2834447138566842	0.0
78-79	3.1996703059716724E-5	7.679208734332015E-4	0.0	2.477760691538344	0.0
80-81	3.1996703059716724E-5	7.679208734332015E-4	0.0	2.685771258129562	0.0
82-83	3.1996703059716724E-5	7.679208734332015E-4	0.0	2.901173063127575	0.0
84-85	3.1996703059716724E-5	7.839192249630598E-4	0.0	3.115998927470513	0.0
86-87	3.1996703059716724E-5	8.319142795526348E-4	0.0	3.342071632938942	0.0
88	3.1996703059716724E-5	8.639109826123516E-4	0.0	3.5213971552371244	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3165	0.0	41.149696	1
GTATCAA	5140	0.0	36.955475	1
CAACGCA	5365	0.0	32.670547	5
ATCAACG	5535	0.0	31.92283	3
TATCAAC	6480	0.0	28.935371	2
AACGCAG	6380	0.0	28.79781	6
GTACTAG	1275	0.0	28.394947	1
GTACATG	11495	0.0	27.159367	1
TACATGG	11515	0.0	26.730589	2
ACGCAGA	6670	0.0	26.488565	7
ACATGGG	11685	0.0	25.376505	3
CGCAGAG	6960	0.0	25.251465	8
TGATCGC	1380	0.0	24.858105	9
GGCGTAA	95	0.0013067428	24.73146	6
TCAACGC	7185	0.0	24.65692	4
GTGATCG	1460	0.0	23.817879	8
TAACGCC	1030	0.0	23.724133	4
CTAACGC	1005	0.0	23.379494	3
CATGGGG	9600	0.0	23.202316	4
GTATAAG	2195	0.0	22.919746	1
>>END_MODULE