FastQCFastQC Report
Mon 16 Jan 2017
ERR1146569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125322
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126500.4047582937054166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113580.3634185533522626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65410.20929043471360712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61350.060.679191
GTATCAA119400.044.7372631
ATCAACG147450.035.460793
CAACGCA149200.035.107895
TATCAAC154800.034.2927322
GTGGTAT13800.033.4214131
AACGCAG160050.033.2113726
TCAACGC164500.031.9854564
ACGCAGA172450.030.3322727
CGCAGAG174750.029.9330468
GCAGAGT195800.026.5469959
GTACTAG14250.025.430431
GTACATG109000.024.1790561
GAGTACT136450.023.57614912-13
TACATGG111000.023.4458562
CAGAGTA191100.022.98219910-11
AGAGTAC182850.022.96532210-11
ACATGGG109350.022.618063
AGTACTT143800.021.34161412-13
TGGTATC21250.021.2606352
Produced by FastQC (version 0.11.5)