##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146569_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3125322
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48324844608012	34.0	31.0	34.0	31.0	34.0
2	32.52039149885996	34.0	31.0	34.0	31.0	34.0
3	32.65621718338142	34.0	31.0	34.0	31.0	34.0
4	36.16466975242871	37.0	35.0	37.0	35.0	37.0
5	36.18640127321281	37.0	37.0	37.0	35.0	37.0
6	36.277989915919065	37.0	37.0	37.0	35.0	37.0
7	36.2671062373733	37.0	37.0	37.0	35.0	37.0
8	36.284670827517935	37.0	37.0	37.0	35.0	37.0
9	38.088545436278245	39.0	38.0	39.0	37.0	39.0
10-11	38.06065535007273	39.0	38.0	39.0	36.0	39.0
12-13	38.03725200155376	39.0	38.0	39.0	35.0	39.0
14-15	39.48769534787135	41.0	39.0	41.0	36.5	41.0
16-17	39.49048658026277	41.0	39.0	41.0	36.5	41.0
18-19	39.540333764008956	41.0	39.5	41.0	37.0	41.0
20-21	39.56770454372381	41.0	40.0	41.0	37.0	41.0
22-23	39.524668658141465	41.0	39.0	41.0	36.5	41.0
24-25	39.50524473958204	41.0	39.0	41.0	36.0	41.0
26-27	39.42728765228031	41.0	39.0	41.0	36.0	41.0
28-29	39.34100054330402	41.0	39.0	41.0	36.0	41.0
30-31	39.227056924054544	41.0	39.0	41.0	35.5	41.0
32-33	39.09817276427837	41.0	39.0	41.0	35.0	41.0
34-35	39.01375506267834	41.0	39.0	41.0	35.0	41.0
36-37	38.925077959967005	41.0	39.0	41.0	35.0	41.0
38-39	38.81471525174046	40.0	38.0	41.0	35.0	41.0
40-41	38.712291885444124	40.0	38.0	41.0	35.0	41.0
42-43	38.59660316600977	40.0	38.0	41.0	35.0	41.0
44-45	38.418928193638926	40.0	38.0	41.0	35.0	41.0
46-47	38.14813497617206	40.0	38.0	41.0	34.0	41.0
48-49	37.88469988052431	40.0	37.5	41.0	33.0	41.0
50-51	37.70409816972459	40.0	37.0	41.0	33.0	41.0
52-53	37.555226789431615	40.0	37.0	41.0	33.0	41.0
54-55	37.39855077332832	40.0	36.0	41.0	33.0	41.0
56-57	37.17664867812021	39.5	35.5	41.0	33.0	41.0
58-59	36.9404387771884	39.0	35.0	41.0	32.0	41.0
60-61	36.74029316019278	39.0	35.0	41.0	32.0	41.0
62-63	36.46168426805302	38.5	35.0	41.0	32.0	41.0
64-65	36.157111171264916	37.0	35.0	40.5	31.0	41.0
66-67	35.89379686317122	37.0	35.0	40.0	31.0	41.0
68-69	35.58387855715347	36.5	35.0	39.5	31.0	41.0
70-71	35.327791664346904	36.0	35.0	39.0	31.0	41.0
72-73	35.02382058552686	35.5	35.0	39.0	31.0	41.0
74-75	34.70998108354915	35.0	35.0	37.5	31.0	40.0
76-77	34.43133651508548	35.0	35.0	37.0	30.0	39.0
78-79	34.1708991905474	35.0	35.0	37.0	30.0	39.0
80-81	33.94987892447563	35.0	34.5	36.0	30.0	39.0
82-83	33.72258410493383	35.0	34.0	36.0	30.0	37.0
84-85	33.53847747528095	35.0	34.0	35.5	30.0	37.0
86-87	33.37619563680158	35.0	34.0	35.0	30.0	37.0
88-89	33.250347644178746	35.0	34.0	35.0	29.0	36.0
90-91	33.176641798829046	35.0	34.0	35.0	29.0	36.0
92-93	33.10124700750835	35.0	34.0	35.0	29.0	36.0
94-95	33.03748157789821	35.0	34.0	35.0	29.0	36.0
96-97	32.99673393653518	35.0	34.0	35.0	29.0	35.0
98-99	32.951389808794104	35.0	34.0	35.0	29.0	35.0
100	32.40447736265255	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2369531569671608
1101	2	0.20535203929939172
1101	3	0.23510162860591777
1101	4	0.17589608967583104
1101	5	0.1546004667302654
1101	6	0.1628526342344827
1101	7	0.18896003273295037
1101	8	0.15754113384383572
1101	9	0.19634394337865047
1101	10-11	0.18570198579900676
1101	12-13	0.23588014717133632
1101	14-15	0.33865031166659065
1101	16-17	0.29395848055076357
1101	18-19	0.2850968863389909
1101	20-21	0.2953435233100308
1101	22-23	0.29497662278178893
1101	24-25	0.2778217301169903
1101	26-27	0.2946231841621767
1101	28-29	0.35596681996126733
1101	30-31	0.3354714453836465
1101	32-33	0.3616228261593619
1101	34-35	0.3492431386772239
1101	36-37	0.33733386433975454
1101	38-39	0.35966376439058223
1101	40-41	0.2979436889295073
1101	42-43	0.30970321863100025
1101	44-45	0.3979616103041792
1101	46-47	0.4474047680104505
1101	48-49	0.4705250266761567
1101	50-51	0.42137265481208175
1101	52-53	0.45746353187311684
1101	54-55	0.40164854908974945
1101	56-57	0.3646937596052737
1101	58-59	0.36055617722837496
1101	60-61	0.3591036045291318
1101	62-63	0.3491340362450899
1101	64-65	0.34758021256563154
1101	66-67	0.37102289941104516
1101	68-69	0.28493092658235497
1101	70-71	0.27010827014237293
1101	72-73	0.2681850805206807
1101	74-75	0.2840525133193097
1101	76-77	0.2179923314768999
1101	78-79	0.262253831273604
1101	80-81	0.26870757829051684
1101	82-83	0.260409481625274
1101	84-85	0.2916397161828286
1101	86-87	0.31301119599701366
1101	88-89	0.3172721585013534
1101	90-91	0.2789341784749908
1101	92-93	0.2463221201240131
1101	94-95	0.25327877817352373
1101	96-97	0.2546567284187802
1101	98-99	0.2751885597245334
1101	100	0.2686479127217396
1102	1	0.2414649556259718
1102	2	0.21429929189405783
1102	3	0.24037867933407142
1102	4	0.16165812973196125
1102	5	0.07793183519468272
1102	6	0.134599852705648
1102	7	0.1505969598512351
1102	8	0.1555722942824218
1102	9	0.16226092772719625
1102	10-11	0.2199268317769878
1102	12-13	0.22636310665617287
1102	14-15	0.29631806520777815
1102	16-17	0.2761255203981321
1102	18-19	0.284465583462449
1102	20-21	0.2737488859943866
1102	22-23	0.302355620005585
1102	24-25	0.2771244302656015
1102	26-27	0.3237774831642142
1102	28-29	0.3659456588163934
1102	30-31	0.39108677121678426
1102	32-33	0.3339793284616803
1102	34-35	0.32228842800326873
1102	36-37	0.33821224978302666
1102	38-39	0.37707661800231307
1102	40-41	0.36604041438462076
1102	42-43	0.3843660414702086
1102	44-45	0.34646348499126134
1102	46-47	0.4039187476376611
1102	48-49	0.35548917112734557
1102	50-51	0.3602322331028489
1102	52-53	0.38531873126159155
1102	54-55	0.3687678876231928
1102	56-57	0.34101781112887863
1102	58-59	0.4057814615417783
1102	60-61	0.4662011730850679
1102	62-63	0.35955918189866765
1102	64-65	0.3463861004824338
1102	66-67	0.3523339639091603
1102	68-69	0.3179132907708677
1102	70-71	0.3487614734261655
1102	72-73	0.3924072396938314
1102	74-75	0.34052946966637165
1102	76-77	0.35314703692884564
1102	78-79	0.3361495972036934
1102	80-81	0.28461444350399745
1102	82-83	0.3108476245317604
1102	84-85	0.36690890502886475
1102	86-87	0.2990666729217182
1102	88-89	0.34212919413381826
1102	90-91	0.3186947688838089
1102	92-93	0.2768222279932715
1102	94-95	0.29321263320181146
1102	96-97	0.23633754442776223
1102	98-99	0.24023254306850816
1102	100	0.267782612366922
1103	1	0.1460437280281468
1103	2	0.17587795977176768
1103	3	0.1469207095836893
1103	4	0.10397468028035917
1103	5	0.033430903485204055
1103	6	0.09301683564893182
1103	7	0.11711224625427974
1103	8	0.1123303417653645
1103	9	0.15284916019442107
1103	10-11	0.17809112381594616
1103	12-13	0.18592625445256772
1103	14-15	0.2026643223307545
1103	16-17	0.24820481241906833
1103	18-19	0.2257240940969112
1103	20-21	0.24623759554972224
1103	22-23	0.2315131107455599
1103	24-25	0.24799385157249532
1103	26-27	0.20966680016288564
1103	28-29	0.29230958297728193
1103	30-31	0.25709427294984977
1103	32-33	0.23228167689819657
1103	34-35	0.27687032412517
1103	36-37	0.2812860020071781
1103	38-39	0.2889496964641438
1103	40-41	0.2803357272431697
1103	42-43	0.29935157624502295
1103	44-45	0.3065152546385619
1103	46-47	0.3516758267674831
1103	48-49	0.31981463989555436
1103	50-51	0.43779037934339016
1103	52-53	0.4373475571875005
1103	54-55	0.37662121937064086
1103	56-57	0.36220080583898095
1103	58-59	0.34634899936581576
1103	60-61	0.37207016187691977
1103	62-63	0.35245115766340973
1103	64-65	0.35060804797751643
1103	66-67	0.3148385743993316
1103	68-69	0.2670321915579237
1103	70-71	0.20484936288493572
1103	72-73	0.16035204837200467
1103	74-75	0.21895423788036084
1103	76-77	0.22032428159179318
1103	78-79	0.2637934054505493
1103	80-81	0.22032003171844394
1103	82-83	0.30565801474442367
1103	84-85	0.2781542660047194
1103	86-87	0.2440166693774941
1103	88-89	0.2564220740952834
1103	90-91	0.24535971149747127
1103	92-93	0.22833098462066204
1103	94-95	0.24892201192999863
1103	96-97	0.21827826944311113
1103	98-99	0.1430813454223525
1103	100	0.19245897698190362
1104	1	0.156455131203721
1104	2	0.2008894305523512
1104	3	0.19213788776004748
1104	4	0.13848564170019984
1104	5	0.05718657063160748
1104	6	0.058057090828789626
1104	7	0.0680650470413795
1104	8	0.08605516848398054
1104	9	0.1041426576847897
1104	10-11	0.13467723946697419
1104	12-13	0.16972167148368555
1104	14-15	0.20591511250781735
1104	16-17	0.2009122651158961
1104	18-19	0.23333993736481773
1104	20-21	0.17209781697354032
1104	22-23	0.2352765431748196
1104	24-25	0.2635688193671868
1104	26-27	0.3085489943751085
1104	28-29	0.3111710020086704
1104	30-31	0.2895142998292286
1104	32-33	0.2950055023193059
1104	34-35	0.23674355893175658
1104	36-37	0.22753787363200217
1104	38-39	0.26394507911196285
1104	40-41	0.26345082534592734
1104	42-43	0.2779085636637504
1104	44-45	0.2529012908362773
1104	46-47	0.2746923981250333
1104	48-49	0.2869816358752644
1104	50-51	0.2182750967788536
1104	52-53	0.2070739021147432
1104	54-55	0.19698072234606911
1104	56-57	0.19030935833681895
1104	58-59	0.2535689409839108
1104	60-61	0.21861702132240168
1104	62-63	0.23938108890841647
1104	64-65	0.27526877423183294
1104	66-67	0.19861238570702255
1104	68-69	0.30863572537145956
1104	70-71	0.2393596854258604
1104	72-73	0.17708910787951027
1104	74-75	0.21581867822823853
1104	76-77	0.2077278834749734
1104	78-79	0.1797129613019237
1104	80-81	0.22445023872683123
1104	82-83	0.24429378174180272
1104	84-85	0.19389296104225195
1104	86-87	0.20120089185603973
1104	88-89	0.14433791167135013
1104	90-91	0.17687343055104776
1104	92-93	0.1926893817547679
1104	94-95	0.13028010588701733
1104	96-97	0.16685075257961302
1104	98-99	0.21940214046651363
1104	100	0.29529623825898454
1105	1	0.07206505386825057
1105	2	0.066404522049055
1105	3	0.07657801650067597
1105	4	0.06631441408065797
1105	5	0.06876480695557063
1105	6	0.012249118979895002
1105	7	0.08641376051109972
1105	8	0.08504623026284719
1105	9	0.12077827315621192
1105	10-11	0.11893923659447836
1105	12-13	0.08180158448725194
1105	14-15	0.10832395051996002
1105	16-17	0.10011952263334933
1105	18-19	0.11185177764370735
1105	20-21	0.14630462058823923
1105	22-23	0.1544826762875644
1105	24-25	0.1719516546444524
1105	26-27	0.19239979277147512
1105	28-29	0.15832381604116108
1105	30-31	0.14120611483184575
1105	32-33	0.21680907401899674
1105	34-35	0.1761922682924677
1105	36-37	0.17976360037278027
1105	38-39	0.16006954062669365
1105	40-41	0.16522964103030802
1105	42-43	0.15275220611069784
1105	44-45	0.20416479683210298
1105	46-47	0.2603556540974097
1105	48-49	0.2696909405206753
1105	50-51	0.25048865467884696
1105	52-53	0.22544034699019022
1105	54-55	0.17483377188772664
1105	56-57	0.1952105524673584
1105	58-59	0.18099212073035176
1105	60-61	0.13832958780322713
1105	62-63	0.1459573646910286
1105	64-65	0.05926768295406504
1105	66-67	0.0018543821274050742
1105	68-69	0.11838100682673058
1105	70-71	0.04595827942094388
1105	72-73	0.09599715164146971
1105	74-75	0.13137304425774943
1105	76-77	0.07735206898497182
1105	78-79	0.07105365943302644
1105	80-81	0.09404910124122523
1105	82-83	0.059305025258026944
1105	84-85	0.09335944746440816
1105	86-87	0.09702397256143769
1105	88-89	0.1151326141643807
1105	90-91	0.08792943450588808
1105	92-93	0.04981021873657454
1105	94-95	0.11141683657892543
1105	96-97	0.07947484454847853
1105	98-99	0.042118051387745936
1105	100	0.019014127559167093
1106	1	0.14535215825271308
1106	2	0.10677952927233036
1106	3	0.09823210839076069
1106	4	0.08906744848354009
1106	5	0.11460266896718707
1106	6	0.0719467716045159
1106	7	0.09528931670314478
1106	8	0.046163111617758545
1106	9	0.07314491896960362
1106	10-11	0.11390143689145305
1106	12-13	0.08426742154528455
1106	14-15	0.12919767765358614
1106	16-17	0.12725129272472913
1106	18-19	0.15525241932161293
1106	20-21	0.09448242114550709
1106	22-23	0.09136646826733852
1106	24-25	0.093732423904207
1106	26-27	0.08117555473258165
1106	28-29	0.14316868525594373
1106	30-31	0.11567567533021617
1106	32-33	0.11756479171086909
1106	34-35	0.09660287683125546
1106	36-37	0.14664731697927635
1106	38-39	0.15773917448838404
1106	40-41	0.14795742538230883
1106	42-43	0.158080720578603
1106	44-45	0.030984345647603106
1106	46-47	0.05796441146829068
1106	48-49	0.07646275292158577
1106	50-51	0.14408418655044386
1106	52-53	0.1951011923267174
1106	54-55	0.18889933157205974
1106	56-57	0.18857679880233746
1106	58-59	0.14653522294406685
1106	60-61	0.14065651189478956
1106	62-63	0.1682720531857953
1106	64-65	0.2324546162411707
1106	66-67	0.20118996660955446
1106	68-69	0.2061670421667543
1106	70-71	0.18997374099438957
1106	72-73	0.13086385398482747
1106	74-75	0.14944185504317176
1106	76-77	0.09912819022542152
1106	78-79	0.13012212098887943
1106	80-81	0.14301085934825153
1106	82-83	0.0533170950335915
1106	84-85	0.1023917800917431
1106	86-87	0.0964021178258605
1106	88-89	0.12068061564168886
1106	90-91	0.16569876210080992
1106	92-93	0.1227954028609517
1106	94-95	0.1185732034860294
1106	96-97	0.13149437125449737
1106	98-99	0.11015055737364321
1106	100	0.11274236666333337
1107	1	0.04415202624483783
1107	2	0.05398003641048632
1107	3	0.13039354737154696
1107	4	0.08166166435439948
1107	5	0.047343185649801
1107	6	0.04676816922599869
1107	7	0.039053677901371486
1107	8	0.060408707991818744
1107	9	0.08907499278480202
1107	10-11	0.05372856830813788
1107	12-13	0.04431106011912078
1107	14-15	0.11093388983487529
1107	16-17	0.13842784850636036
1107	18-19	0.15394376069730242
1107	20-21	0.11310849459980687
1107	22-23	0.1413480678893606
1107	24-25	0.10151583127433383
1107	26-27	0.1352697694163183
1107	28-29	0.1459202896501921
1107	30-31	0.10546180358203827
1107	32-33	0.07137397860410033
1107	34-35	0.04345681581797578
1107	36-37	0.07265329532143738
1107	38-39	0.13888042528672884
1107	40-41	0.09837472221245491
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2216	34-35	-0.5222385556788041
2216	36-37	-0.5428080689522758
2216	38-39	-0.5374399072414633
2216	40-41	-0.49158322386983855
2216	42-43	-0.5657963341703649
2216	44-45	-0.567556238902192
2216	46-47	-0.5847724620422099
2216	48-49	-0.5375041662040729
2216	50-51	-0.5091628829722339
2216	52-53	-0.5106971088771033
2216	54-55	-0.5456365548681248
2216	56-57	-0.5190151054956971
2216	58-59	-0.5186683018962484
2216	60-61	-0.5421665595258247
2216	62-63	-0.5487318505227279
2216	64-65	-0.46060046702716306
2216	66-67	-0.4444277970615147
2216	68-69	-0.4367541791260763
2216	70-71	-0.5069000390966991
2216	72-73	-0.48374173126590847
2216	74-75	-0.45722577616781024
2216	76-77	-0.39526797508190015
2216	78-79	-0.5007988091433617
2216	80-81	-0.43060596613872804
2216	82-83	-0.4289831094977359
2216	84-85	-0.4551983269187545
2216	86-87	-0.4801726475382466
2216	88-89	-0.4386756319095042
2216	90-91	-0.4147225220970796
2216	92-93	-0.4432027746894889
2216	94-95	-0.4588851623482455
2216	96-97	-0.41838486899612093
2216	98-99	-0.40238998044667795
2216	100	-0.4673773326864108
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	19.0
8	73.0
9	113.0
10	194.0
11	214.0
12	182.0
13	215.0
14	342.0
15	690.0
16	1105.0
17	2119.0
18	3889.0
19	6284.0
20	9482.0
21	13748.0
22	20405.0
23	25797.0
24	25024.0
25	21047.0
26	17152.0
27	17490.0
28	20370.0
29	24859.0
30	30481.0
31	39357.0
32	51351.0
33	68599.0
34	103268.0
35	172997.0
36	318936.0
37	783678.0
38	990287.0
39	354650.0
40	904.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.574497258500884	19.790503709463493	13.728102805816746	23.906896226218873
2	15.510889885570862	20.85268825537033	39.06038351405755	24.57603834500126
3	17.517633462045982	26.59312298179891	30.99412921862421	24.895114337530895
4	12.5966860374707	17.955909823051833	38.51385553232595	30.93354860715152
5	12.949692101551753	38.29416129282138	34.84322754652407	13.912919059102801
6	29.48848648212784	38.50312108424559	17.87721474109144	14.131177692535124
7	25.718702174912593	33.698522487770056	22.96503303180697	17.61774230551038
8	26.336646271968135	33.695312035047905	20.61963535277325	19.348406340210705
9	26.798038730089253	14.297502785313002	20.831421530325514	38.07303695427223
10-11	24.866285777913443	25.900323230694312	28.216532568484144	21.0168584229081
12-13	25.940879051822503	23.998247220606387	28.04442230272593	22.016451424845187
14-15	23.130448638572283	24.663698652490847	26.478071699492084	25.72778100944479
16-17	21.85413215022324	27.499214480939884	27.24909625312208	23.397557115714797
18-19	21.740687839525016	26.367171126687104	30.143390025091815	21.74875100869606
20-21	22.918918434644496	25.53202518012544	29.720201630424004	21.828854754806066
22-23	23.055871613293792	25.300930340305662	29.471543284693862	22.171654761706687
24-25	22.53193430948875	25.567157560085008	29.83852543833883	22.062382692087407
26-27	22.46211750341245	25.891044186806994	29.291317822611557	22.355520487169002
28-29	22.17012623185366	25.916813971559076	29.64772732908513	22.265332467502134
30-31	22.515602004851022	25.620692540394668	29.57419233457571	22.28951312017859
32-33	22.68785650908353	25.92208803652547	29.045161203620424	22.344894250770572
34-35	22.70703891680815	25.835460608034733	29.45436603800873	22.003134437148393
36-37	22.680626288776484	25.6779070293903	29.240813560428368	22.400653121404844
38-39	22.730692032100567	25.73324890418625	29.41015639123631	22.125902672476876
40-41	22.594544834673027	25.45213199536105	29.42406855841395	22.52925461155197
42-43	22.66561247470443	25.681210494798822	28.978893389611653	22.674283640885093
44-45	22.66979915592373	26.09360351453136	28.73273371281569	22.50386361672922
46-47	22.945419814354416	25.913141416371495	28.223056272817736	22.918382496456353
48-49	23.410656068427375	25.963851778661816	27.913953192958495	22.711538959952314
50-51	23.132477075022805	26.260893743742606	27.490710500709852	23.115918680524736
52-53	22.838654002416742	26.560623918397084	27.410467636424958	23.19025444276122
54-55	22.902411580938438	26.56063541318864	27.269773349436456	23.26717965643647
56-57	23.31845846847393	26.286290779956893	27.186429950841433	23.20882080072774
58-59	22.855632118441914	26.348846250453967	27.736193482430977	23.059328148673146
60-61	23.04228594210687	26.589688500967203	27.35218542722146	23.015840129704472
62-63	23.20775146770286	26.223453680488095	27.241326570445256	23.32746828136379
64-65	22.6255194294991	26.380994907979005	27.376056477963612	23.617429184558283
66-67	23.104850012223267	26.14701899533724	27.38760036196222	23.360530630477268
68-69	22.794871007233425	26.071398630621772	27.52969876622849	23.60403159591631
70-71	22.917202620703247	25.936847655945027	27.395538558613215	23.750411164738512
72-73	23.19339592001559	25.932216568820976	27.531111217061277	23.343276294102154
74-75	23.112561325927956	26.402070902492074	27.192210437226965	23.293157334352998
76-77	23.20309303965285	26.208906568053575	26.938553419041693	23.64944697325188
78-79	22.835507444966474	26.405149330488165	27.02593783411798	23.733405390427382
80-81	22.903986967096653	26.434392888665336	27.230151275685166	23.43146886855284
82-83	23.179854091717665	25.98244755456202	27.717246188749662	23.120452164970654
84-85	23.345317704216257	26.322916321702046	26.935397505631826	23.396368468449875
86-87	22.66306042434354	26.57649780990532	27.228174765380135	23.532267000371
88-89	23.01116365684154	26.06785865127411	27.18657609460791	23.734401597276438
90-91	22.972528910621048	26.03469338519359	27.254983646485066	23.737794057700295
92-93	23.178139724482786	26.086016096901375	27.165936821869874	23.56990735674596
94-95	22.4983253722857	26.154493225736935	27.514129346372844	23.83305205560452
96-97	22.72594340332006	26.07918198116217	27.217390163590327	23.97748445192744
98-99	22.581419279532486	26.274899977730165	27.182123723004224	23.96155701973312
100	22.93198920550936	25.93538280189601	26.894619329100777	24.238008663493847
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	424.0
1	290.0
2	407.5
3	611.5
4	387.5
5	324.5
6	699.0
7	1799.0
8	3495.0
9	4211.0
10	2976.5
11	1700.0
12	1618.0
13	1891.0
14	2229.0
15	2667.0
16	3114.5
17	3674.0
18	4363.5
19	5013.0
20	5749.5
21	6666.5
22	7845.5
23	9266.0
24	10942.0
25	13261.0
26	15592.5
27	18225.0
28	21578.5
29	25690.5
30	31610.5
31	37818.0
32	44747.5
33	50502.5
34	54824.5
35	60884.5
36	67902.5
37	76807.5
38	81122.5
39	81451.0
40	82012.5
41	81650.0
42	82719.0
43	87684.5
44	95079.5
45	107458.5
46	117842.0
47	124647.5
48	131009.5
49	128465.0
50	124042.5
51	123568.0
52	121579.0
53	120263.0
54	119059.0
55	109421.0
56	100749.5
57	90773.0
58	79793.0
59	73442.0
60	61574.5
61	47554.0
62	36603.5
63	28924.5
64	22233.5
65	15848.5
66	11483.5
67	8507.0
68	6712.5
69	5128.0
70	3978.0
71	3063.0
72	2192.0
73	1659.0
74	1293.0
75	1038.0
76	778.5
77	489.0
78	344.0
79	248.0
80	125.5
81	57.5
82	29.0
83	10.5
84	6.0
85	4.5
86	2.0
87	2.0
88	1.0
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006623317533361362
2	0.08050370489824728
3	4.4795384283603416E-4
4	0.0
5	0.03967591179404874
6	0.004031584585524307
7	3.199670305971673E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.4795384283603416E-4
24-25	0.0
26-27	0.0
28-29	4.799505458957509E-5
30-31	7.999175764929182E-5
32-33	0.001631831856045553
34-35	0.001279868122388669
36-37	7.199258188436263E-4
38-39	6.079373581346178E-4
40-41	0.0014558499892171112
42-43	6.879291157839097E-4
44-45	3.8396043671660074E-4
46-47	3.8396043671660074E-4
48-49	7.359241703734846E-4
50-51	6.079373581346178E-4
52-53	0.0013278631769782442
54-55	0.0015518400983962613
56-57	8.799093341422101E-4
58-59	0.002463746135598188
60-61	0.002031790644292012
62-63	9.599010917915019E-4
64-65	0.0044315433737707665
66-67	0.004287558210002041
68-69	0.0034556439304494068
70-71	0.0017278219652247034
72-73	0.005215462598733826
74-75	0.0036636225003375653
76-77	0.003567632391158415
78-79	7.199258188436263E-4
80-81	4.799505458957509E-5
82-83	7.999175764929182E-5
84-85	4.799505458957509E-5
86-87	1.5998351529858365E-5
88-89	6.399340611943346E-5
90-91	0.0
92-93	0.0
94-95	1.5998351529858365E-5
96-97	4.7995054589575095E-4
98-99	5.75940655074901E-4
100	7.679208734332015E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3125322.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.40358034502006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.72151165406429	33.12599493051802
2	16.911166836032887	17.047667126960448
3	7.096465483404733	10.730618044753564
4	3.666473989664017	7.392136652838261
5	2.059227483042305	5.189621894509814
6	1.264430153145774	3.8239084088849435
7	0.8394685065271946	2.961855282110022
8	0.5589802749027127	2.2539685757872228
9	0.3972951045811539	1.802258615199541
>10	1.4451147201234147	11.143388227628332
>50	0.025982620096221265	0.8668926526735805
>100	0.011570015552834156	1.090635280674465
>500	0.001366866600471605	0.4903202055620298
>1k	6.308615079099701E-4	0.461755290862728
>5k	1.0514358465166168E-4	0.34664769396684303
>10k+	2.1028716930332337E-4	1.2723311170701679
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12650	0.4047582937054166	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11358	0.3634185533522626	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6541	0.20929043471360712	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	6.399340611943345E-5	0.0	0.0	0.0
14-15	0.0	6.399340611943345E-5	0.0	3.1996703059716724E-5	0.0
16-17	0.0	6.399340611943345E-5	0.0	3.1996703059716724E-5	0.0
18-19	0.0	6.399340611943345E-5	0.0	4.7995054589575086E-5	0.0
20-21	0.0	1.5998351529858363E-4	0.0	1.279868122388669E-4	3.1996703059716724E-5
22-23	0.0	1.5998351529858363E-4	0.0	2.2397692141801708E-4	3.1996703059716724E-5
24-25	0.0	1.5998351529858363E-4	0.0	6.239357096644762E-4	3.1996703059716724E-5
26-27	0.0	1.5998351529858363E-4	0.0	0.001279868122388669	3.1996703059716724E-5
28-29	0.0	1.5998351529858363E-4	0.0	0.004271559858472183	3.1996703059716724E-5
30-31	0.0	1.9198021835830037E-4	0.0	0.011006865852542555	3.1996703059716724E-5
32-33	0.0	1.9198021835830037E-4	0.0	0.02559736244777338	3.1996703059716724E-5
34-35	0.0	1.9198021835830037E-4	0.0	0.04804304964416466	3.1996703059716724E-5
36-37	0.0	2.0797856988815874E-4	0.0	0.08512722849037635	3.1996703059716724E-5
38-39	0.0	2.3997527294787542E-4	0.0	0.13673791052569945	3.1996703059716724E-5
40-41	0.0	2.559736244777338E-4	0.0	0.18876454970079884	3.1996703059716724E-5
42-43	0.0	2.559736244777338E-4	0.0	0.2425670058957125	3.1996703059716724E-5
44-45	0.0	2.559736244777338E-4	0.0	0.3085762043079081	3.1996703059716724E-5
46-47	0.0	2.559736244777338E-4	0.0	0.3906797443591412	3.1996703059716724E-5
48-49	0.0	2.559736244777338E-4	0.0	0.4773748112994437	3.1996703059716724E-5
50-51	0.0	2.559736244777338E-4	0.0	0.5657657035019112	3.1996703059716724E-5
52-53	0.0	2.559736244777338E-4	0.0	0.6477892517954951	3.1996703059716724E-5
54-55	0.0	3.8396043671660074E-4	0.0	0.7387878752973294	3.1996703059716724E-5
56-57	0.0	4.159571397763174E-4	0.0	0.845000931104059	3.1996703059716724E-5
58-59	0.0	4.4795384283603416E-4	0.0	0.9634207291280706	3.1996703059716724E-5
60-61	0.0	4.799505458957509E-4	0.0	1.082304479346448	3.1996703059716724E-5
62-63	0.0	4.959488974256093E-4	0.0	1.2000843433092654	3.1996703059716724E-5
64-65	0.0	5.119472489554676E-4	0.0	1.323607615471302	3.1996703059716724E-5
66-67	0.0	5.75940655074901E-4	0.0	1.463097242460137	3.1996703059716724E-5
68-69	0.0	5.75940655074901E-4	0.0	1.6162654600070008	3.1996703059716724E-5
70-71	0.0	5.919390066047594E-4	0.0	1.7790966818778995	3.1996703059716724E-5
72-73	0.0	6.079373581346178E-4	0.0	1.943351757034955	3.1996703059716724E-5
74-75	0.0	6.079373581346178E-4	0.0	2.108726716799101	3.1996703059716724E-5
76-77	0.0	6.079373581346178E-4	0.0	2.2924677841195242	3.1996703059716724E-5
78-79	0.0	6.399340611943345E-4	0.0	2.487503687620028	3.1996703059716724E-5
80-81	0.0	6.399340611943345E-4	0.0	2.696554147060687	3.1996703059716724E-5
82-83	0.0	6.399340611943345E-4	0.0	2.9130118432596706	3.1996703059716724E-5
84-85	0.0	6.399340611943345E-4	0.0	3.128589630124512	3.1996703059716724E-5
86-87	0.0	7.03927467313768E-4	0.0	3.35586221195768	3.1996703059716724E-5
88	0.0	7.03927467313768E-4	0.0	3.535795671613997	3.1996703059716724E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6135	0.0	60.67919	1
GTATCAA	11940	0.0	44.737263	1
ATCAACG	14745	0.0	35.46079	3
CAACGCA	14920	0.0	35.10789	5
TATCAAC	15480	0.0	34.292732	2
GTGGTAT	1380	0.0	33.421413	1
AACGCAG	16005	0.0	33.211372	6
TCAACGC	16450	0.0	31.985456	4
ACGCAGA	17245	0.0	30.332272	7
CGCAGAG	17475	0.0	29.933046	8
GCAGAGT	19580	0.0	26.546995	9
GTACTAG	1425	0.0	25.43043	1
GTACATG	10900	0.0	24.179056	1
GAGTACT	13645	0.0	23.576149	12-13
TACATGG	11100	0.0	23.445856	2
CAGAGTA	19110	0.0	22.982199	10-11
AGAGTAC	18285	0.0	22.965322	10-11
ACATGGG	10935	0.0	22.61806	3
AGTACTT	14380	0.0	21.341614	12-13
TGGTATC	2125	0.0	21.260635	2
>>END_MODULE