FastQCFastQC Report
Mon 16 Jan 2017
ERR1146568_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146568_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4102084
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53800.033.4672781
GGTATCA35700.033.1845781
CAACGCA53300.031.2093035
ATCAACG54900.030.2997423
AACGCAG64300.027.0395076
TCAACGC62850.026.8409064
TATCAAC67650.025.6310622
GTACATG116050.025.2375321
ACGCAGA67200.024.9635877
TACATGG113250.024.5635722
CGCAGAG69650.023.9508278
ACATGGG112050.023.9040243
GTGATCG10700.021.9583428
GTACTAG15300.021.2012581
CATGGGG88950.020.4443384
TGATCGC11000.020.077919
GTATAAG20950.019.7470661
GCATCGT13700.018.5219428
GCAGAGT90750.018.0714139
CTAGTAC21000.017.9010583
Produced by FastQC (version 0.11.5)