##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146568_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4102084 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.222286525580657 31.0 31.0 34.0 28.0 34.0 2 31.318482995472547 31.0 31.0 34.0 28.0 34.0 3 31.54302910423092 33.0 31.0 34.0 28.0 34.0 4 35.07765199347454 37.0 35.0 37.0 32.0 37.0 5 35.06747350858734 37.0 35.0 37.0 32.0 37.0 6 35.155369563373156 37.0 35.0 37.0 32.0 37.0 7 35.00192170613766 37.0 35.0 37.0 32.0 37.0 8 34.98559463921266 37.0 35.0 37.0 32.0 37.0 9 36.591159274164056 39.0 37.0 39.0 32.0 39.0 10-11 36.621431813682996 39.0 37.0 39.0 32.0 39.0 12-13 36.60714980483091 39.0 37.0 39.0 32.0 39.0 14-15 37.977355290627884 40.0 38.0 41.0 33.0 41.0 16-17 37.96495269233882 40.0 38.0 41.0 32.5 41.0 18-19 37.966817841857946 40.0 38.0 41.0 32.0 41.0 20-21 38.086303693439724 40.0 38.0 41.0 32.5 41.0 22-23 38.084506558129966 40.0 38.0 41.0 32.0 41.0 24-25 38.138888915975386 40.0 38.0 41.0 32.0 41.0 26-27 38.18053482083741 40.0 38.0 41.0 32.5 41.0 28-29 38.14219101802888 40.0 38.0 41.0 32.0 41.0 30-31 38.13092625602011 40.0 38.0 41.0 32.0 41.0 32-33 38.15556080762852 40.0 38.0 41.0 33.0 41.0 34-35 38.09261182852423 40.0 38.0 41.0 32.0 41.0 36-37 37.998024784475405 40.0 38.0 41.0 32.0 41.0 38-39 37.95614473033707 40.0 38.0 41.0 32.0 41.0 40-41 37.903264413893034 40.0 38.0 41.0 32.0 41.0 42-43 37.76223036875891 40.0 38.0 41.0 31.5 41.0 44-45 37.5953709139062 40.0 37.0 41.0 31.0 41.0 46-47 37.48717773697467 40.0 37.0 41.0 31.0 41.0 48-49 37.37393029981834 40.0 37.0 41.0 31.0 41.0 50-51 37.25147351931359 40.0 36.5 41.0 30.0 41.0 52-53 37.144850885549886 40.0 36.0 41.0 30.0 41.0 54-55 36.977792385528915 39.0 36.0 41.0 30.0 41.0 56-57 36.85664055148554 39.0 35.0 41.0 30.0 41.0 58-59 36.6511539012853 39.0 35.0 41.0 30.0 41.0 60-61 36.483004492350716 39.0 35.0 40.0 30.0 41.0 62-63 36.258143421733934 38.0 35.0 40.0 30.0 41.0 64-65 36.019330418392215 38.0 35.0 40.0 29.0 41.0 66-67 35.76868501473885 37.0 35.0 40.0 29.0 41.0 68-69 35.485625477196464 37.0 34.0 40.0 29.0 41.0 70-71 35.22208028894582 36.0 34.0 39.0 29.0 41.0 72-73 34.903423723161204 36.0 34.0 39.0 29.0 40.5 74-75 34.57915586321489 35.0 34.0 38.5 29.0 40.0 76-77 34.26595006343117 35.0 34.0 37.0 28.5 39.5 78-79 33.97955965796898 35.0 34.0 37.0 28.0 39.0 80-81 33.691133945574975 35.0 34.0 36.5 28.0 39.0 82-83 33.43274893931962 35.0 33.0 36.0 28.0 37.5 84-85 33.1964539243955 35.0 33.0 36.0 27.0 37.0 86-87 32.98990586248355 35.0 33.0 35.0 27.0 37.0 88-89 32.8369280102504 35.0 33.0 35.0 27.0 36.0 90-91 32.67257008876464 35.0 33.0 35.0 27.0 36.0 92-93 32.57964561427801 35.0 33.0 35.0 27.0 36.0 94-95 32.48142261348134 35.0 33.0 35.0 26.5 36.0 96-97 32.40393309839584 35.0 33.0 35.0 26.0 35.0 98-99 32.33344112407254 35.0 33.0 35.0 26.0 35.0 100 31.368009772593638 34.0 31.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3431488138357821 1101 2 0.33235170710605644 1101 3 0.42717982673115884 1101 4 0.3146505848509662 1101 5 0.29346962732729054 1101 6 0.36760445032623323 1101 7 0.35843897858588036 1101 8 0.34346924625437936 1101 9 0.5406444886982982 1101 10-11 0.5168915548773896 1101 12-13 0.481000064734161 1101 14-15 0.5741040129877248 1101 16-17 0.5817712115547806 1101 18-19 0.5596614683872971 1101 20-21 0.5548349967202881 1101 22-23 0.5375505271343215 1101 24-25 0.5535771266444627 1101 26-27 0.5878331620062909 1101 28-29 0.6149776984757906 1101 30-31 0.6309324432762295 1101 32-33 0.5976312769775021 1101 34-35 0.6085147614511754 1101 36-37 0.5940884185603892 1101 38-39 0.6014691836196633 1101 40-41 0.5888953700321338 1101 42-43 0.5840588636393704 1101 44-45 0.5606821485638704 1101 46-47 0.5269547321139498 1101 48-49 0.4747261551446087 1101 50-51 0.5261312635462332 1101 52-53 0.5318926022873995 1101 54-55 0.5450860226455276 1101 56-57 0.5139413758792202 1101 58-59 0.5499810123865743 1101 60-61 0.514103888711368 1101 62-63 0.5232958117561637 1101 64-65 0.4783845580200392 1101 66-67 0.4758816214244206 1101 68-69 0.4451170857776887 1101 70-71 0.42299995387395484 1101 72-73 0.436940828282502 1101 74-75 0.43599770359421797 1101 76-77 0.4299773700746101 1101 78-79 0.4255190307997836 1101 80-81 0.4317805839364155 1101 82-83 0.43233656177405067 1101 84-85 0.4971530037326417 1101 86-87 0.44938737745648893 1101 88-89 0.4040974987137247 1101 90-91 0.41595072671047717 1101 92-93 0.4128019656682582 1101 94-95 0.4079748459502923 1101 96-97 0.35530048365687605 1101 98-99 0.318905554908838 1101 100 0.25240345215157944 1102 1 0.35449513187551673 1102 2 0.35964831270367625 1102 3 0.44090349772532633 1102 4 0.36145142055723056 1102 5 0.39916081011748616 1102 6 0.4196823250010624 1102 7 0.39540835202794966 1102 8 0.48556827863130536 1102 9 0.5561612801062381 1102 10-11 0.4970967901150658 1102 12-13 0.5658192353397169 1102 14-15 0.7053027931650462 1102 16-17 0.6255020461069591 1102 18-19 0.6061366409017168 1102 20-21 0.5943354396062475 1102 22-23 0.6343420862427323 1102 24-25 0.6412718594481461 1102 26-27 0.6043171369665288 1102 28-29 0.6104728879970835 1102 30-31 0.6090349108579218 1102 32-33 0.5970162778309103 1102 34-35 0.5674155372158296 1102 36-37 0.6315281814417659 1102 38-39 0.6012934743345184 1102 40-41 0.6091087637479262 1102 42-43 0.5909510661760677 1102 44-45 0.5450304868774651 1102 46-47 0.5197079977194647 1102 48-49 0.47955995853440925 1102 50-51 0.5274537447917567 1102 52-53 0.44761204706870217 1102 54-55 0.42988774219246295 1102 56-57 0.3959540060371083 1102 58-59 0.42385993753454443 1102 60-61 0.4995970510995775 1102 62-63 0.49375309408858215 1102 64-65 0.43848704611279743 1102 66-67 0.5171481474037876 1102 68-69 0.4762773977084578 1102 70-71 0.46092813662439625 1102 72-73 0.45067202444219845 1102 74-75 0.4301945023888081 1102 76-77 0.4324336540240168 1102 78-79 0.4193796111924897 1102 80-81 0.34288961619279945 1102 82-83 0.39422434544032114 1102 84-85 0.4443762043373667 1102 86-87 0.40908637016979554 1102 88-89 0.3487358882057663 1102 90-91 0.43735980596252944 1102 92-93 0.4071878425708064 1102 94-95 0.3510788009175627 1102 96-97 0.34812034364751554 1102 98-99 0.31039182904022766 1102 100 0.3047440083785169 1103 1 0.2682883184321412 1103 2 0.2669063280565638 1103 3 0.34968368321931464 1103 4 0.25823212286129404 1103 5 0.3072843027744483 1103 6 0.2854492241850082 1103 7 0.25422184946643966 1103 8 0.29974660589627433 1103 9 0.4416705511667942 1103 10-11 0.4233886900811257 1103 12-13 0.4311648322322057 1103 14-15 0.5497436680787757 1103 16-17 0.4344875568052089 1103 18-19 0.4276159029521196 1103 20-21 0.41468963787058755 1103 22-23 0.4434300516092762 1103 24-25 0.36993768322667364 1103 26-27 0.4043205678747057 1103 28-29 0.5065055483525782 1103 30-31 0.46167929514647454 1103 32-33 0.43191365161720086 1103 34-35 0.4778718111418101 1103 36-37 0.5048339106141952 1103 38-39 0.46305079495213164 1103 40-41 0.483633268702377 1103 42-43 0.443131379965223 1103 44-45 0.3809088357581061 1103 46-47 0.37585375139406096 1103 48-49 0.36862787529925356 1103 50-51 0.2846998842875692 1103 52-53 0.32933777308620193 1103 54-55 0.36937758032448187 1103 56-57 0.31944168203538226 1103 58-59 0.376219934506274 1103 60-61 0.33868169236032486 1103 62-63 0.34958921538923704 1103 64-65 0.37320938687305727 1103 66-67 0.34081596025842487 1103 68-69 0.2815911277965526 1103 70-71 0.24053264459200108 1103 72-73 0.2704802073755701 1103 74-75 0.22477657854948774 1103 76-77 0.24523319097428242 1103 78-79 0.2351295401405551 1103 80-81 0.24958365829316165 1103 82-83 0.27166932827585555 1103 84-85 0.2394831716423269 1103 86-87 0.26615308167455964 1103 88-89 0.2485721781218615 1103 90-91 0.19653481526519556 1103 92-93 0.2800096530147371 1103 94-95 0.1625077703908886 1103 96-97 0.18902596541266092 1103 98-99 0.2035784423737539 1103 100 0.1918692088338183 1104 1 0.25021206308375454 1104 2 0.22921339913105498 1104 3 0.2775064962404201 1104 4 0.3034791003938295 1104 5 0.25649867594556497 1104 6 0.2745561442759552 1104 7 0.21090032705870243 1104 8 0.2918630025138711 1104 9 0.3632824339755629 1104 10-11 0.38636804207160225 1104 12-13 0.36064480147255296 1104 14-15 0.4730433350417087 1104 16-17 0.42529335747445174 1104 18-19 0.3433457337127095 1104 20-21 0.4343367138455463 1104 22-23 0.4491197205316695 1104 24-25 0.39058307651462343 1104 26-27 0.3809920953164294 1104 28-29 0.3611407682945327 1104 30-31 0.38833310439552093 1104 32-33 0.3454968204889042 1104 34-35 0.40699195853119363 1104 36-37 0.3483133450610296 1104 38-39 0.38214654103419576 1104 40-41 0.3842590619162678 1104 42-43 0.2962263621081007 1104 44-45 0.2525397178101514 1104 46-47 0.22659078851529557 1104 48-49 0.29776859166995706 1104 50-51 0.28611346046479014 1104 52-53 0.2208027915639974 1104 54-55 0.24686823136160996 1104 56-57 0.22353591972547093 1104 58-59 0.2479532103908042 1104 60-61 0.2024480063591767 1104 62-63 0.26450179000141105 1104 64-65 0.16706782284379784 1104 66-67 0.17545722820260323 1104 68-69 0.17198019909982776 1104 70-71 0.15766288016457253 1104 72-73 0.09999822020989768 1104 74-75 0.16911592271653575 1104 76-77 0.2055870658636323 1104 78-79 0.24634622064618128 1104 80-81 0.23429544717025408 1104 82-83 0.2263437285186427 1104 84-85 0.24038874810968025 1104 86-87 0.18946095818705544 1104 88-89 0.12095292584402273 1104 90-91 0.23764629986570895 1104 92-93 0.16649120426235697 1104 94-95 0.17936743364418817 1104 96-97 0.18057202387259252 1104 98-99 0.1945538469241228 1104 100 0.10830203866013832 1105 1 0.27440466372779326 1105 2 0.293923738696904 1105 3 0.330708920035228 1105 4 0.3450767588166741 1105 5 0.28363589928841293 1105 6 0.2539577205786969 1105 7 0.21575848419892196 1105 8 0.26723202388000544 1105 9 0.3292155868481288 1105 10-11 0.38184119417773843 1105 12-13 0.3416496799518498 1105 14-15 0.3703190335723576 1105 16-17 0.3781261212067193 1105 18-19 0.3436511757544949 1105 20-21 0.39445238757154755 1105 22-23 0.39585621413483807 1105 24-25 0.33672883400276277 1105 26-27 0.36954642341361676 1105 28-29 0.4466488629005667 1105 30-31 0.43201180990886456 1105 32-33 0.42342239842205487 1105 34-35 0.40944998166128954 1105 36-37 0.4376206381611354 1105 38-39 0.4213729229119352 1105 40-41 0.39990755941200007 1105 42-43 0.4319692647394149 1105 44-45 0.37966648975721284 1105 46-47 0.3368980711640006 1105 48-49 0.36149966448250836 1105 50-51 0.3466510802639178 1105 52-53 0.34187660861115887 1105 54-55 0.43357861389430497 1105 56-57 0.3321843336100372 1105 58-59 0.34082210692081816 1105 60-61 0.31915444639312796 1105 62-63 0.30111143269142104 1105 64-65 0.343739059092961 1105 66-67 0.29846469862805947 1105 68-69 0.30224088013599726 1105 70-71 0.28588823896679827 1105 72-73 0.27255770316773464 1105 74-75 0.327797084307889 1105 76-77 0.2509027793049441 1105 78-79 0.26099582598985904 1105 80-81 0.26710687844245484 1105 82-83 0.19042178767121953 1105 84-85 0.2233676049360085 1105 86-87 0.23215360353984238 1105 88-89 0.21320890607462673 1105 90-91 0.22838465033352406 1105 92-93 0.2242356014432616 1105 94-95 0.2829666330672751 1105 96-97 0.23956469512162215 1105 98-99 0.2005852022727339 1105 100 0.13845692834565781 1106 1 0.28905200031583433 1106 2 0.262281501407994 1106 3 0.2356807366891296 1106 4 0.35401596739630037 1106 5 0.29233643551516053 1106 6 0.305598700192931 1106 7 0.18234321145501298 1106 8 0.2217527926622367 1106 9 0.26819349750749666 1106 10-11 0.3336265205443141 1106 12-13 0.31726179593233184 1106 14-15 0.3603561063917198 1106 16-17 0.38618309958614816 1106 18-19 0.3335261800420213 1106 20-21 0.36125520704950276 1106 22-23 0.41519153306969514 1106 24-25 0.3353443914384755 1106 26-27 0.38316318135697713 1106 28-29 0.41238165848269404 1106 30-31 0.4151995578708565 1106 32-33 0.37586000060812097 1106 34-35 0.35711199302609486 1106 36-37 0.3329199361499917 1106 38-39 0.2953860770456842 1106 40-41 0.3094112924190995 1106 42-43 0.32035363870326705 1106 44-45 0.31223283209626373 1106 46-47 0.284757308784485 1106 48-49 0.26432300120775665 1106 50-51 0.32828931586067966 1106 52-53 0.36910012026751815 1106 54-55 0.3551798910057684 1106 56-57 0.2558756373613349 1106 58-59 0.293919559557942 1106 60-61 0.327795814743169 1106 62-63 0.3095245616716724 1106 64-65 0.3108207107628047 1106 66-67 0.28073863160543056 1106 68-69 0.2546125973192588 1106 70-71 0.22501378348275836 1106 72-73 0.20285251354469125 1106 74-75 0.23230170340396938 1106 76-77 0.2218872241558998 1106 78-79 0.24310568958932777 1106 80-81 0.257071063297758 1106 82-83 0.2769070728775347 1106 84-85 0.28006172130520923 1106 86-87 0.2102441961332815 1106 88-89 0.2393186610567355 1106 90-91 0.26049351350995664 1106 92-93 0.23471974902567183 1106 94-95 0.22514861287588417 1106 96-97 0.2591292538493093 1106 98-99 0.21713548639287694 1106 100 0.2266616549157483 1107 1 0.20742739893518802 1107 2 0.2011532369797706 1107 3 0.243768758817815 1107 4 0.143337328162346 1107 5 0.18323587118131712 1107 6 0.09895557105006958 1107 7 0.15325506072186101 1107 8 0.22014207620456716 1107 9 0.3269074855320184 1107 10-11 0.26099834657516396 1107 12-13 0.2060170518225206 1107 14-15 0.3500668498781181 1107 16-17 0.3060349701272216 1107 18-19 0.2638519035511351 1107 20-21 0.28322526400602044 1107 22-23 0.2736189947008896 1107 24-25 0.26716012848375925 1107 26-27 0.28981254999622763 1107 28-29 0.2751899277499348 1107 30-31 0.23140393870573917 1107 32-33 0.25218010366607757 1107 34-35 0.17658377197463437 1107 36-37 0.19711206577457574 1107 38-39 0.21188589394780166 1107 40-41 0.23327150808225383 1107 42-43 0.23168780651247545 1107 44-45 0.2561045022759316 1107 46-47 0.2761269409103164 1107 48-49 0.2977203007319176 1107 50-51 0.24561250106636834 1107 52-53 0.19903913123377492 1107 54-55 0.2224353136458248 1107 56-57 0.2507521389274743 1107 58-59 0.24581960881999265 1107 60-61 0.25419961969160454 1107 62-63 0.22833702475238482 1107 64-65 0.2247525798216614 1107 66-67 0.24679669761222556 1107 68-69 0.1408627362774837 1107 70-71 0.19306628135206694 1107 72-73 0.1768095095187192 1107 74-75 0.13671428070288272 1107 76-77 0.12974580174689976 1107 78-79 0.11846567311051359 1107 80-81 0.18573255781245734 1107 82-83 0.10382668527675065 1107 84-85 0.10064434858549731 1107 86-87 0.13844522126346703 1107 88-89 0.08168328850703688 1107 90-91 0.12299008042460002 1107 92-93 0.11763502701839457 1107 94-95 0.06641819508574542 1107 96-97 0.027374903639724835 1107 98-99 0.040378482353830236 1107 100 0.09406598082126649 1108 1 0.24541398810339388 1108 2 0.2744759639358705 1108 3 0.26516613492534447 1108 4 0.2593314483877407 1108 5 0.2292214050053616 1108 6 0.18427244834300183 1108 7 0.2043907080748042 1108 8 0.1899708756433398 1108 9 0.255003244660287 1108 10-11 0.24283022923995645 1108 12-13 0.20319222219578847 1108 14-15 0.19566589509793175 1108 16-17 0.18901039666430108 1108 18-19 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-0.6876804230697502 2215 28-29 -0.6410049676881613 2215 30-31 -0.6709210501547886 2215 32-33 -0.7437444295798912 2215 34-35 -0.6959657646326463 2215 36-37 -0.6108711662556914 2215 38-39 -0.6337025033449493 2215 40-41 -0.6643804115379837 2215 42-43 -0.5792416989704776 2215 44-45 -0.5669979784141788 2215 46-47 -0.5550012354092075 2215 48-49 -0.5470704904389052 2215 50-51 -0.5237851709164403 2215 52-53 -0.546386063092271 2215 54-55 -0.4544448231491245 2215 56-57 -0.5184660533529595 2215 58-59 -0.5016703979093435 2215 60-61 -0.46869008645006716 2215 62-63 -0.47513165819665915 2215 64-65 -0.47270160636124103 2215 66-67 -0.4828762941397571 2215 68-69 -0.44618121250047693 2215 70-71 -0.4677648516620323 2215 72-73 -0.4668076142438693 2215 74-75 -0.4174473111769501 2215 76-77 -0.42421002723026646 2215 78-79 -0.4872013785963105 2215 80-81 -0.43178337944060274 2215 82-83 -0.3693708170359571 2215 84-85 -0.3752297887705538 2215 86-87 -0.3310064119096765 2215 88-89 -0.39772446282130147 2215 90-91 -0.34844184005512346 2215 92-93 -0.3485444419612733 2215 94-95 -0.42018834678815153 2215 96-97 -0.40272403512213373 2215 98-99 -0.4148058757618358 2215 100 -0.3426035695278351 2216 1 -0.9798076820535471 2216 2 -1.0288244234078938 2216 3 -1.060202718413624 2216 4 -0.9903937181801012 2216 5 -0.883037230414871 2216 6 -0.9391298890894149 2216 7 -0.9163047884361362 2216 8 -0.9160743472433452 2216 9 -1.1514398045331404 2216 10-11 -1.1787460491694048 2216 12-13 -1.2271350372853576 2216 14-15 -1.4715217033914314 2216 16-17 -1.4799195453272347 2216 18-19 -1.4897648449785308 2216 20-21 -1.5638962029684365 2216 22-23 -1.5540471116648646 2216 24-25 -1.5989282816772104 2216 26-27 -1.5369173779077059 2216 28-29 -1.5912230515544863 2216 30-31 -1.579595261146551 2216 32-33 -1.5404558379681887 2216 34-35 -1.5265526104604135 2216 36-37 -1.4818564133719647 2216 38-39 -1.5060160448325703 2216 40-41 -1.547956826345441 2216 42-43 -1.496298231958761 2216 44-45 -1.4141957962533667 2216 46-47 -1.2897072410573998 2216 48-49 -1.3288422893575955 2216 50-51 -1.3821207059664644 2216 52-53 -1.3277034568184547 2216 54-55 -1.3292566314618028 2216 56-57 -1.2261958567387197 2216 58-59 -1.213185025114079 2216 60-61 -1.2328009428666888 2216 62-63 -1.2030083831268357 2216 64-65 -1.1511016862579737 2216 66-67 -1.193142702979614 2216 68-69 -1.1487448809556327 2216 70-71 -1.182128264964554 2216 72-73 -1.135356309830371 2216 74-75 -1.0983443693622519 2216 76-77 -1.1033210618010614 2216 78-79 -1.094956680064918 2216 80-81 -1.033079340883404 2216 82-83 -0.9976311411450922 2216 84-85 -1.0132201880159286 2216 86-87 -0.9812710362198729 2216 88-89 -1.0322310515752129 2216 90-91 -0.9818574865930572 2216 92-93 -0.923569852274241 2216 94-95 -0.8566781762016049 2216 96-97 -0.9242002407833141 2216 98-99 -0.8836093430230356 2216 100 -1.0424688745139647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 125.0 1 59.0 2 45.0 3 38.0 4 35.0 5 64.0 6 130.0 7 639.0 8 1887.0 9 2516.0 10 2854.0 11 3803.0 12 5472.0 13 7065.0 14 8460.0 15 9175.0 16 10274.0 17 11152.0 18 12388.0 19 13913.0 20 15403.0 21 17413.0 22 19968.0 23 23684.0 24 30176.0 25 38553.0 26 41590.0 27 37789.0 28 39022.0 29 48440.0 30 55641.0 31 63282.0 32 80872.0 33 109570.0 34 162834.0 35 253145.0 36 479770.0 37 998441.0 38 1182540.0 39 313547.0 40 310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42217647317095 19.941117068244687 13.249025528946303 24.387680929638066 2 14.932742816546627 20.8459235861656 40.31759374974096 23.90373984754681 3 17.786187945384892 26.07280632740637 30.716889719232437 25.424116007976306 4 12.07941198986371 17.847510957657047 40.14671484833842 29.926362204140823 5 13.138009884336538 38.45080542153738 35.66699279629787 12.74419189782821 6 29.677129148340665 38.756131693663995 18.80925678509084 12.757482372904496 7 26.32111299043042 32.94426710366123 23.37707992134488 17.35753998456347 8 25.04270635023376 35.69487809034413 21.2250621609913 18.037353398430806 9 25.96529713583861 15.802475697219315 20.848926104041304 37.38330106290077 10-11 23.85673072105774 26.656903474462005 29.646712462002967 19.839653342477284 12-13 24.659338650056224 24.551740530920593 28.67145517100568 22.117465648017504 14-15 22.27640452862389 25.84550870830603 26.056092766696633 25.821993996373443 16-17 22.1474050784747 28.609499856644945 26.809162728466024 22.43393233641433 18-19 22.009563428874575 28.406665266458653 27.954762960345303 21.62900834432147 20-21 23.128430589414407 27.658682797504458 27.308981941183504 21.903904671897635 22-23 23.066782545947433 27.546544709005673 26.9380200245248 22.44865272052209 24-25 22.743328949615442 27.584499118279084 27.31328476178786 22.35888717031762 26-27 22.649035215485018 27.84664669774758 26.974752640530568 22.529565446236834 28-29 22.49981502165557 27.881337885283685 27.203177409896046 22.415669683164705 30-31 22.91222036771837 27.61604505030321 27.103919179389358 22.367815402589056 32-33 22.768562596249247 27.849754812416595 26.822791431411048 22.558891159923107 34-35 22.89970075136422 27.863768484832523 27.045698684856617 22.190832078946638 36-37 22.927746099180283 27.60668165441643 26.964185812499032 22.501386433904255 38-39 22.79072670643592 27.657225980085464 27.205542792745568 22.346504520733053 40-41 22.83657570898483 27.41748153255784 27.293119723446452 22.45282303501088 42-43 22.67851584678694 27.465732055513403 27.170518540210985 22.68523355748867 44-45 22.71593278828916 27.75214145398434 27.109693066390438 22.42223269133606 46-47 22.75201095578147 27.48722611917997 26.914259329502176 22.846503595536387 48-49 22.8421980562353 27.415312359402606 27.141183255482765 22.601306328879332 50-51 22.733420146509427 27.563390688555295 26.98066943688487 22.72251972805041 52-53 22.647081021350207 27.57970862702921 26.968051440362085 22.8051589112585 54-55 22.67987862685195 27.469838089878806 27.093927623609115 22.756355659660134 56-57 22.852998028027084 27.3915353928411 27.0030913589737 22.752375220158118 58-59 22.735212253720462 27.379106396515922 27.243577433007047 22.64210391675656 60-61 22.706686807044516 27.628024226982124 27.043090783874952 22.622198182098412 62-63 22.821830690451204 27.414669797327623 26.966103486658415 22.797396025562755 64-65 22.494869722032213 27.435080493238274 27.074260087717693 22.99578969701182 66-67 22.739769389444024 27.34156727043534 26.94398640962632 22.974676930494315 68-69 22.605964984544975 27.318220101152395 27.05109065561519 23.02472425868744 70-71 22.671366130813134 27.071181517717186 27.07338858313107 23.18406376833861 72-73 22.79199270845082 27.198002749572474 27.13773163319118 22.872272908785522 74-75 22.6841024976945 27.37127460494964 27.05968009191026 22.8849428054456 76-77 22.601995252325676 27.21358974221406 27.060170699718622 23.12424430574164 78-79 22.565228243524306 27.29347951070389 27.042622520648784 23.098669725123024 80-81 22.587050953797725 27.324024176833735 27.118286721093444 22.970638148275103 82-83 22.813659189447 27.04202380111895 27.42675535294718 22.717561656486865 84-85 22.945015149944826 27.18278088363501 27.012455266510997 22.85974869990916 86-87 22.497455328641806 27.40251360368871 27.13899928939412 22.96103177827536 88-89 22.83140309188967 27.038360934889816 27.089655485218767 23.040580488001744 90-91 22.771365289897087 27.04545357555894 27.201645563678106 22.981535570865866 92-93 22.868414885473566 27.086348593363667 27.161592413335484 22.883644107827283 94-95 22.483689618360543 27.05471789334678 27.35206951780043 23.109522970492247 96-97 22.61471462218744 27.059800686731307 27.177170018853957 23.1483146722273 98-99 22.504053767855144 27.22126299543664 27.12122189384572 23.153461342862496 100 22.699388597475824 26.932314109662297 27.04138775424608 23.3269095386158 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 919.0 1 739.0 2 559.5 3 513.0 4 432.0 5 360.5 6 315.5 7 317.0 8 321.5 9 335.0 10 329.0 11 318.0 12 327.0 13 337.0 14 373.0 15 463.5 16 601.0 17 751.0 18 990.5 19 1352.5 20 1947.5 21 2893.0 22 4542.5 23 6764.0 24 9465.0 25 13252.5 26 18663.0 27 24931.0 28 31117.0 29 38588.0 30 47156.5 31 56135.5 32 66645.0 33 76499.5 34 85663.0 35 95072.0 36 106359.5 37 119287.5 38 124433.0 39 126567.0 40 130087.5 41 131096.5 42 133271.5 43 139284.0 44 147230.5 45 156099.0 46 161839.5 47 165682.5 48 168347.0 49 165610.0 50 160323.5 51 157206.0 52 152889.5 53 146535.5 54 138345.0 55 125754.5 56 115032.0 57 101978.0 58 88674.5 59 79127.5 60 65211.0 61 50327.0 62 39451.0 63 30602.0 64 22792.0 65 16150.5 66 11806.0 67 8806.5 68 6476.5 69 4741.0 70 3581.5 71 2775.5 72 2130.5 73 1726.5 74 1376.0 75 984.5 76 726.0 77 526.0 78 351.0 79 258.0 80 176.0 81 111.5 82 75.5 83 62.5 84 51.0 85 39.5 86 30.0 87 21.0 88 15.0 89 17.0 90 17.0 91 12.0 92 6.0 93 5.5 94 8.0 95 6.5 96 5.5 97 6.0 98 6.0 99 10.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05909191523137996 2 0.008922294131470736 3 0.010604365975928332 4 0.009409851187835256 5 0.010799388798474141 6 0.010823766651292368 7 0.0030716094550964825 8 0.005728795412283122 9 0.007069577317285556 10-11 0.006911121273967086 12-13 0.006009140719692723 14-15 0.00966581864242663 16-17 0.0092635840709259 18-19 0.007618079005695642 20-21 0.007130521949331121 22-23 0.0027790752212777698 24-25 0.003961401082961734 26-27 0.005107160165418358 28-29 0.006155407836602078 30-31 0.008727271308924927 32-33 0.010445909932609864 34-35 0.011652613647112054 36-37 0.017905532894987038 38-39 0.018478412436215348 40-41 0.018149311423169296 42-43 0.02399999609954355 44-45 0.027595729390231895 46-47 0.03011883715691829 48-49 0.03117927375451112 50-51 0.03227627713133129 52-53 0.03065514991891926 54-55 0.03672523527065755 56-57 0.03954087727116266 58-59 0.036188922508656574 60-61 0.037346870517522314 62-63 0.03297104593665074 64-65 0.034835951677245035 66-67 0.037407815149567876 68-69 0.03991873398984516 70-71 0.039406799080662414 72-73 0.03526256410156398 74-75 0.038090395028478205 76-77 0.03778567186825038 78-79 0.03755408226647723 80-81 0.04174707295121211 82-83 0.038090395028478205 84-85 0.03434839462088051 86-87 0.03310512412715098 88-89 0.030679527771737488 90-91 0.03319044661201477 92-93 0.03449466173778987 94-95 0.03782223864747772 96-97 0.03299542378946896 98-99 0.03094768415273797 100 0.02888775558959787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4102084.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.407964118648415 #Duplication Level Percentage of deduplicated Percentage of total 1 71.5416593893187 42.50144333989212 2 15.004121929334394 17.82728674419447 3 5.965801381387518 10.632483432133583 4 2.92525168046135 6.951329874834554 5 1.5434414160843315 4.584635613298693 6 0.9082373279941063 3.237391835561459 7 0.584659694612269 2.4313409511401924 8 0.3775736756209314 1.7944706698747588 9 0.2710845242361227 1.449412172004641 >10 0.8605590153884146 7.641892251679606 >50 0.013727045725248164 0.534164655173729 >100 0.0037471068582335897 0.37009514610708927 >500 1.3581297838579257E-4 0.044053314105175725 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4626711690935633E-4 2 0.0 0.0 0.0 0.0 1.4626711690935633E-4 3 0.0 0.0 0.0 0.0 1.4626711690935633E-4 4 0.0 0.0 0.0 0.0 1.4626711690935633E-4 5 0.0 0.0 0.0 0.0 1.7064496972758237E-4 6 0.0 0.0 0.0 0.0 2.4377852818226054E-4 7 0.0 0.0 0.0 0.0 2.4377852818226054E-4 8 0.0 0.0 0.0 0.0 2.4377852818226054E-4 9 0.0 0.0 0.0 0.0 2.6815638100048656E-4 10-11 0.0 0.0 0.0 0.0 2.9253423381871266E-4 12-13 0.0 0.0 0.0 0.0 9.01980554274364E-4 14-15 0.0 0.0 0.0 1.2188926409113026E-5 0.0011091923032292854 16-17 0.0 0.0 0.0 2.4377852818226052E-5 0.001377348684229772 18-19 0.0 0.0 0.0 4.8755705636452105E-5 0.0014748600955026762 20-21 0.0 0.0 0.0 4.8755705636452105E-5 0.0017795832557305018 22-23 0.0 0.0 0.0 1.5845604331846935E-4 0.001889283593412519 24-25 0.0 0.0 0.0 4.1442349790984294E-4 0.001901472519821632 26-27 0.0 0.0 0.0 0.0011701369352748506 0.0020721174895492146 28-29 0.0 0.0 0.0 0.003851700745279716 0.0021330621215947796 30-31 0.0 0.0 0.0 0.009824274685745099 0.0021818178272312314 32-33 0.0 0.0 0.0 0.023658706160088385 0.002291518164913249 34-35 0.0 0.0 0.0 0.04558658477008272 0.0024621631346408313 36-37 0.0 0.0 0.0 0.08062974819628267 0.0024865409874590574 38-39 0.0 0.0 0.0 0.12927575349505277 0.0025109188402772835 40-41 0.0 0.0 0.0 0.18089585683764642 0.0025352966930955096 42-43 0.0 0.0 0.0 0.23269879407637678 0.0025962413251410747 44-45 0.0 0.0 0.0 0.2982752181574049 0.00277907522127777 46-47 0.0 0.0 0.0 0.37665001496800166 0.0029740980438235786 48-49 0.0 0.0 0.0 0.4563168379779644 0.0031203651607329347 50-51 0.0 0.0 0.0 0.5362396284425185 0.003169120866369387 52-53 0.0 0.0 0.0 0.6162599303183456 0.0034250883209607603 54-55 0.0 0.0 0.0 0.7026184739269112 0.0035104108058245515 56-57 0.0 0.0 0.0 0.8044447651486415 0.0036323000699156815 58-59 0.0 0.0 0.0 0.9150470833849331 0.003754189334006812 60-61 0.0 0.0 0.0 1.0316463534145084 0.0038517007452797164 62-63 0.0 0.0 0.0 1.1487453694268572 0.004095479273461977 64-65 0.0 0.0 0.0 1.2680262032664373 0.004119857126280203 66-67 0.0 0.0 0.0 1.401714348121589 0.004217368537553107 68-69 0.0 0.0 0.0 1.5500657714469035 0.00435144672805335 70-71 0.0 0.0 0.0 1.7034390324527728 0.0044367692129171415 72-73 0.0 0.0 0.0 1.8583968538918265 0.004790248078781419 74-75 0.0 0.0 0.0 2.0187178029508903 0.005046215533372793 76-77 0.0 0.0 0.0 2.1924953267656146 0.005143726944645698 78-79 0.0 0.0 0.0 2.3783886434309975 0.005253427282327715 80-81 0.0 0.0 0.0 2.5771047106787672 0.005338749767191506 82-83 0.0 0.0 0.0 2.7811839055465466 0.005655661853828445 84-85 0.0 0.0 0.0 2.984848676916416 0.0056800397066466704 86-87 0.0 0.0 0.0 3.1996906938034426 0.005936007161238044 88 0.0 0.0 0.0 3.370896354145844 0.006216352468647644 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5380 0.0 33.467278 1 GGTATCA 3570 0.0 33.184578 1 CAACGCA 5330 0.0 31.209303 5 ATCAACG 5490 0.0 30.299742 3 AACGCAG 6430 0.0 27.039507 6 TCAACGC 6285 0.0 26.840906 4 TATCAAC 6765 0.0 25.631062 2 GTACATG 11605 0.0 25.237532 1 ACGCAGA 6720 0.0 24.963587 7 TACATGG 11325 0.0 24.563572 2 CGCAGAG 6965 0.0 23.950827 8 ACATGGG 11205 0.0 23.904024 3 GTGATCG 1070 0.0 21.958342 8 GTACTAG 1530 0.0 21.201258 1 CATGGGG 8895 0.0 20.444338 4 TGATCGC 1100 0.0 20.07791 9 GTATAAG 2095 0.0 19.747066 1 GCATCGT 1370 0.0 18.521942 8 GCAGAGT 9075 0.0 18.071413 9 CTAGTAC 2100 0.0 17.901058 3 >>END_MODULE