FastQCFastQC Report
Mon 16 Jan 2017
ERR1146568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4102084
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106290.2591121976049247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98550.24024373952361774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57120.1392462952977072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68150.058.2688141
GTATCAA123150.042.0641861
ATCAACG153050.033.3302153
CAACGCA153750.033.2396285
TATCAAC161950.032.0143242
TCAACGC163900.031.468024
AACGCAG165000.031.274526
ACGCAGA179650.028.5148817
CGCAGAG184400.027.8058478
GCAGAGT209100.024.3642399
GTACTAG15300.024.2937871
GTACATG117250.023.5149651
TACATGG114650.023.3086952
GTGGTAT21900.022.7730581
ACATGGG113500.022.4100653
GAGTACT147200.022.39739212-13
CAGAGTA201550.022.11730210-11
AGAGTAC190200.022.00396710-11
CGACGAG6750.020.53953224-25
GTACTTT166000.019.83250814-15
Produced by FastQC (version 0.11.5)