FastQCFastQC Report
Mon 16 Jan 2017
ERR1146567_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146567_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4114092
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT195890.4761439462219124No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA95510.23215329166192686No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC45790.11130037928174673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14850.021.5319331
TGATCGC9550.020.1739339
GGCGTCG9450.018.8956538
CATGGGG68750.018.7973444
TACGGGC9650.018.5051574
GTATAAG24100.017.9502871
CTAGTAC18200.017.8165993
TCTAACG5850.017.6729322
CGGGCGT9250.017.2728166
TAGTACT20500.016.9634594
CGTTAAC3101.2551754E-716.6852051
GCATCGT14150.016.604428
CTAACGC5950.016.5862943
TACCTGG41400.016.572742
GTACATG231550.016.4896641
TCGACGG2901.1497268E-616.20894494
GTACCTG42300.016.1186331
ATACGGG10250.016.0469043
GTGATCG12100.015.9224038
TACATGG232350.015.9174622
Produced by FastQC (version 0.11.5)