##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146567_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4114092
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189547292573913	31.0	31.0	34.0	28.0	34.0
2	31.423093601212614	31.0	31.0	34.0	28.0	34.0
3	31.62005978475931	33.0	31.0	34.0	28.0	34.0
4	35.17065053479601	37.0	35.0	37.0	32.0	37.0
5	35.23119633688308	37.0	35.0	37.0	32.0	37.0
6	35.27571648859578	37.0	35.0	37.0	32.0	37.0
7	35.19447110079211	37.0	35.0	37.0	32.0	37.0
8	35.180267237582434	37.0	35.0	37.0	32.0	37.0
9	36.87340997721976	39.0	37.0	39.0	33.0	39.0
10-11	36.84444793650701	39.0	37.0	39.0	33.0	39.0
12-13	36.80814114025647	39.0	37.0	39.0	33.0	39.0
14-15	38.198222596869485	40.0	38.0	41.0	33.0	41.0
16-17	38.20449323933447	40.0	38.0	41.0	33.0	41.0
18-19	38.23083927146014	40.0	38.5	41.0	33.0	41.0
20-21	38.30538585427841	40.0	39.0	41.0	33.0	41.0
22-23	38.30749032836407	40.0	38.5	41.0	33.0	41.0
24-25	38.34469051737297	40.0	38.0	41.0	33.0	41.0
26-27	38.34473670010296	40.0	38.0	41.0	33.0	41.0
28-29	38.2871814728499	40.0	38.0	41.0	33.0	41.0
30-31	38.2689301794904	40.0	38.0	41.0	33.0	41.0
32-33	38.26194394777754	40.0	38.0	41.0	33.0	41.0
34-35	38.18868209558755	40.0	38.0	41.0	33.0	41.0
36-37	38.098780727314804	40.0	38.0	41.0	33.0	41.0
38-39	38.0530600676893	40.0	38.0	41.0	33.0	41.0
40-41	38.00422219532281	40.0	38.0	41.0	32.0	41.0
42-43	37.88436658684346	40.0	38.0	41.0	32.0	41.0
44-45	37.76145173710262	40.0	38.0	41.0	32.0	41.0
46-47	37.64835715876067	40.0	37.0	41.0	31.5	41.0
48-49	37.53393835140294	40.0	37.0	41.0	31.0	41.0
50-51	37.4122197559024	40.0	37.0	41.0	31.0	41.0
52-53	37.30870627103137	40.0	36.5	41.0	31.0	41.0
54-55	37.13378322604356	40.0	36.0	41.0	30.0	41.0
56-57	36.97956280510985	39.5	35.5	41.0	30.0	41.0
58-59	36.77364045334912	39.0	35.0	41.0	30.0	41.0
60-61	36.578158315370686	39.0	35.0	41.0	30.0	41.0
62-63	36.3487702754338	39.0	35.0	40.5	30.0	41.0
64-65	36.11753942303672	38.0	35.0	40.0	29.0	41.0
66-67	35.83023568748584	37.0	35.0	40.0	29.0	41.0
68-69	35.52756950986998	37.0	34.5	40.0	29.0	41.0
70-71	35.21774087696629	36.5	34.0	39.0	29.0	41.0
72-73	34.88117037732749	36.0	34.0	39.0	28.5	41.0
74-75	34.567938441823856	35.5	34.0	38.5	28.0	40.0
76-77	34.23123680753858	35.0	34.0	37.0	28.0	39.5
78-79	33.92718405422144	35.0	34.0	37.0	28.0	39.0
80-81	33.611550009090706	35.0	34.0	36.5	27.0	39.0
82-83	33.36371743752935	35.0	33.0	36.0	27.0	38.0
84-85	33.133366973806126	35.0	33.0	36.0	27.0	37.0
86-87	32.934540720042236	35.0	33.0	35.0	27.0	37.0
88-89	32.7542520925638	35.0	33.0	35.0	26.0	36.0
90-91	32.56780499804088	35.0	33.0	35.0	26.0	36.0
92-93	32.45363108068561	35.0	33.0	35.0	26.0	36.0
94-95	32.369887693323335	35.0	33.0	35.0	26.0	36.0
96-97	32.314480449148924	35.0	33.0	35.0	25.0	35.5
98-99	32.24590480718467	35.0	33.0	35.0	25.0	35.0
100	31.26609735513936	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1015433342241181
1101	2	0.14988046495688323
1101	3	0.169642528369792
1101	4	0.24620291479782708
1101	5	0.1337178655792357
1101	6	0.23439596647755678
1101	7	0.17667910419794453
1101	8	0.315108378174358
1101	9	0.32629862329770276
1101	10-11	0.3775519298080994
1101	12-13	0.3486495189223646
1101	14-15	0.39470514979625193
1101	16-17	0.353336705356746
1101	18-19	0.3358788407957789
1101	20-21	0.37623203700076857
1101	22-23	0.3529092931877713
1101	24-25	0.32213204423762676
1101	26-27	0.36349961531941233
1101	28-29	0.3963042304196449
1101	30-31	0.4236695456876092
1101	32-33	0.4336470529286558
1101	34-35	0.44190026873055643
1101	36-37	0.3759381043398733
1101	38-39	0.38687461479986496
1101	40-41	0.3704119153230039
1101	42-43	0.3783882208170013
1101	44-45	0.34815734255614217
1101	46-47	0.3225554139530473
1101	48-49	0.3874954849126837
1101	50-51	0.37168500380968084
1101	52-53	0.36410253128109105
1101	54-55	0.4214189376197979
1101	56-57	0.3561148525455877
1101	58-59	0.4122157473304
1101	60-61	0.39807721569473387
1101	62-63	0.4327648209900019
1101	64-65	0.30567210704344205
1101	66-67	0.2764091839291041
1101	68-69	0.27125338908891905
1101	70-71	0.3047488364733084
1101	72-73	0.2740152239590188
1101	74-75	0.3004938799292063
1101	76-77	0.2572050376019632
1101	78-79	0.32468769693847577
1101	80-81	0.2487637071998421
1101	82-83	0.2774802277645634
1101	84-85	0.3093756164587873
1101	86-87	0.3180297759504285
1101	88-89	0.26145686643202026
1101	90-91	0.318409403228479
1101	92-93	0.30846270774961226
1101	94-95	0.27618608610672624
1101	96-97	0.30165431584357094
1101	98-99	0.2885581282109797
1101	100	0.1011874948411311
1102	1	0.09273108255991858
1102	2	0.0706628257424029
1102	3	0.17007242088304153
1102	4	0.16813458422737426
1102	5	0.2247958251426354
1102	6	0.2821405972481088
1102	7	0.2210151068786459
1102	8	0.2861094992185187
1102	9	0.39318341455600603
1102	10-11	0.31503120819891706
1102	12-13	0.3885014471047086
1102	14-15	0.42769095489802567
1102	16-17	0.4188544152664804
1102	18-19	0.3911592244298987
1102	20-21	0.31729385516276665
1102	22-23	0.3375315094770954
1102	24-25	0.37450806533450276
1102	26-27	0.36047500955781686
1102	28-29	0.37742223019353105
1102	30-31	0.3976956130292635
1102	32-33	0.3453064230462175
1102	34-35	0.43502542793108745
1102	36-37	0.41973697975412705
1102	38-39	0.45875997134776014
1102	40-41	0.4306381060748734
1102	42-43	0.3706785903256886
1102	44-45	0.3669994319754224
1102	46-47	0.28732261680482196
1102	48-49	0.284622179249034
1102	50-51	0.3736098829534882
1102	52-53	0.308940143334425
1102	54-55	0.25735626948020496
1102	56-57	0.24503708368228416
1102	58-59	0.3315753832021784
1102	60-61	0.34355500117618476
1102	62-63	0.29116870200947886
1102	64-65	0.21269371881345478
1102	66-67	0.2782147733490632
1102	68-69	0.2659413356166098
1102	70-71	0.20212422417342424
1102	72-73	0.22506240588828064
1102	74-75	0.19291538981087797
1102	76-77	0.19078313678508607
1102	78-79	0.20489156215709414
1102	80-81	0.18988610669748596
1102	82-83	0.2545455098576852
1102	84-85	0.2618098691936339
1102	86-87	0.23027108785815642
1102	88-89	0.22079831087631163
1102	90-91	0.2551314319506375
1102	92-93	0.2309949803363338
1102	94-95	0.1888388334822153
1102	96-97	0.18748264216413446
1102	98-99	0.18344058884162706
1102	100	0.20017692052053349
1103	1	0.11794702944760616
1103	2	0.03906544547137969
1103	3	0.1408998995390327
1103	4	0.046380780171382696
1103	5	0.14552699023332138
1103	6	0.14697301824129738
1103	7	0.1557915604548441
1103	8	0.18928131602220333
1103	9	0.3136982699339299
1103	10-11	0.31756234188873833
1103	12-13	0.28194704511299307
1103	14-15	0.363549187926715
1103	16-17	0.3607982143220738
1103	18-19	0.28746332329775015
1103	20-21	0.3108975459513701
1103	22-23	0.3163591161113857
1103	24-25	0.26122959115247824
1103	26-27	0.30587122113629306
1103	28-29	0.37692011327413866
1103	30-31	0.35828311258398315
1103	32-33	0.3145596366868588
1103	34-35	0.27613522407616387
1103	36-37	0.3139234995492046
1103	38-39	0.2720225142105903
1103	40-41	0.28482850479984023
1103	42-43	0.22952433161689356
1103	44-45	0.22443932505417052
1103	46-47	0.22299617187045584
1103	48-49	0.18526738118427488
1103	50-51	0.19485899733047063
1103	52-53	0.21734643749188365
1103	54-55	0.2862665672474449
1103	56-57	0.26230099340612867
1103	58-59	0.2697235701982592
1103	60-61	0.16416564237385245
1103	62-63	0.17331879680578055
1103	64-65	0.2010871095918887
1103	66-67	0.18777993079535804
1103	68-69	0.22586839458293895
1103	70-71	0.1833479605856283
1103	72-73	0.10298575348393513
1103	74-75	0.12179151605289462
1103	76-77	0.13532064036385805
1103	78-79	0.14348862973886867
1103	80-81	0.1927907523097474
1103	82-83	0.16977463059399867
1103	84-85	0.209468822858895
1103	86-87	0.14633012147686486
1103	88-89	0.10116907852181356
1103	90-91	0.1527118174951525
1103	92-93	0.14841503677872225
1103	94-95	0.09509083726614875
1103	96-97	0.05409999760473028
1103	98-99	0.0783582341464708
1103	100	0.05891836609479384
1104	1	-0.06939435965420415
1104	2	-0.032387733026929766
1104	3	0.04708084411511493
1104	4	-0.0451155597493198
1104	5	0.06064193955081976
1104	6	0.05172144321798555
1104	7	0.04264127953822339
1104	8	0.10527486959200871
1104	9	0.15280501236215827
1104	10-11	0.10300311106411897
1104	12-13	0.0992397100263176
1104	14-15	0.1714832961956816
1104	16-17	0.14075467571102251
1104	18-19	0.09304742355069351
1104	20-21	0.10846003936590165
1104	22-23	0.14723515732075043
1104	24-25	0.08946968160906721
1104	26-27	0.0867575139041179
1104	28-29	0.11333544901682302
1104	30-31	0.15721883338047604
1104	32-33	0.13444346503278126
1104	34-35	0.09820260677570758
1104	36-37	0.12607463837070298
1104	38-39	0.07325339733866087
1104	40-41	0.08967865111837625
1104	42-43	0.020349024263332183
1104	44-45	0.06204136071990263
1104	46-47	0.09285565774820981
1104	48-49	0.0831804269931311
1104	50-51	0.154621017045109
1104	52-53	-0.005390596141936044
1104	54-55	0.06021142336928875
1104	56-57	0.07020676652696523
1104	58-59	0.10114061647618655
1104	60-61	0.14670131675466536
1104	62-63	0.027847848244384465
1104	64-65	0.03217259783777848
1104	66-67	0.0837177959860469
1104	68-69	0.029775661248201857
1104	70-71	-0.012555887094428897
1104	72-73	0.017913427098349644
1104	74-75	0.0769909482704918
1104	76-77	0.04085830961270176
1104	78-79	0.037009037607994344
1104	80-81	0.10140999158093678
1104	82-83	0.07564178793710852
1104	84-85	0.04026485714024375
1104	86-87	0.03144302477587502
1104	88-89	-0.008979547303233915
1104	90-91	0.07289208852882467
1104	92-93	0.05310438419817842
1104	94-95	-0.03093028603479553
1104	96-97	-0.027979612753860295
1104	98-99	0.009182922499704205
1104	100	-0.011358781093051107
1105	1	0.03862953220817644
1105	2	0.07141479657582295
1105	3	0.12355357536161549
1105	4	0.0948687023322492
1105	5	0.15162069009888057
1105	6	0.07933382424761248
1105	7	0.11493235659516188
1105	8	0.1118527382591239
1105	9	0.1327817361379715
1105	10-11	0.20802376504328635
1105	12-13	0.1762278158431272
1105	14-15	0.2408960571357568
1105	16-17	0.26272748769331145
1105	18-19	0.15512873731547216
1105	20-21	0.2663431662681788
1105	22-23	0.1914063521852114
1105	24-25	0.23906216975558436
1105	26-27	0.23056666547255134
1105	28-29	0.2658531230372887
1105	30-31	0.2731658938341539
1105	32-33	0.2440647541334826
1105	34-35	0.2817218597776474
1105	36-37	0.2599726823404964
1105	38-39	0.28294671847982045
1105	40-41	0.20780243442855806
1105	42-43	0.1762526638386106
1105	44-45	0.1376031935839208
1105	46-47	0.12690937573876937
1105	48-49	0.10989374199274238
1105	50-51	0.1334835869401303
1105	52-53	0.08026844439069691
1105	54-55	0.16991386342871095
1105	56-57	0.09598713463767439
1105	58-59	0.11650127858971615
1105	60-61	0.10226877221023756
1105	62-63	0.09809265487215413
1105	64-65	0.0994004214811568
1105	66-67	0.13797141549228797
1105	68-69	0.14727124746935516
1105	70-71	0.08415722476829757
1105	72-73	0.06348269299280673
1105	74-75	0.09786579801222217
1105	76-77	0.1281780163660855
1105	78-79	0.11891290760276974
1105	80-81	0.13536335698815094
1105	82-83	0.11352849123463926
1105	84-85	0.092194753862195
1105	86-87	0.10916909573351319
1105	88-89	0.07199125718256738
1105	90-91	0.09656119723685208
1105	92-93	0.09881692831557842
1105	94-95	0.06752775678437928
1105	96-97	-0.028377004832208286
1105	98-99	0.056271874655934084
1105	100	0.0512830273530227
1106	1	0.033590312328577454
1106	2	0.015240349628395933
1106	3	0.07965163149519938
1106	4	0.12954715310976894
1106	5	0.12909597008647467
1106	6	0.11600531902193012
1106	7	0.03551063635732277
1106	8	0.10981669099138713
1106	9	0.1625332613876438
1106	10-11	0.1693819859051402
1106	12-13	0.17196177279618752
1106	14-15	0.20281849710721644
1106	16-17	0.11168366065090396
1106	18-19	0.10327967619371492
1106	20-21	0.07915970265980121
1106	22-23	0.11334133164442761
1106	24-25	0.09589744134495248
1106	26-27	0.07644135185883272
1106	28-29	0.1017277993017558
1106	30-31	0.10379195870373792
1106	32-33	0.08940551456839074
1106	34-35	0.0746206663139759
1106	36-37	0.12756201064646433
1106	38-39	0.05320162781507776
1106	40-41	0.07272398382455236
1106	42-43	0.029145212198855575
1106	44-45	0.07231759127856918
1106	46-47	0.032171116578844305
1106	48-49	0.07515753900804611
1106	50-51	0.049310757170189845
1106	52-53	0.05826274233626805
1106	54-55	0.06696108301564863
1106	56-57	0.023918915891286474
1106	58-59	0.0660851344619573
1106	60-61	0.06658020727739711
1106	62-63	-0.011285167036753307
1106	64-65	-0.02676695320675293
1106	66-67	8.232698022965224E-4
1106	68-69	-0.003764814691912477
1106	70-71	0.03971097285618441
1106	72-73	0.14040004385829263
1106	74-75	0.09936732647918234
1106	76-77	0.08522301204479277
1106	78-79	0.009484924850923449
1106	80-81	0.03621500683740919
1106	82-83	0.05399558136929983
1106	84-85	0.11059476034016313
1106	86-87	0.05675905448421048
1106	88-89	0.04734817059459573
1106	90-91	0.01413805401586643
1106	92-93	0.005426736387100561
1106	94-95	0.014266036766329648
1106	96-97	0.037902372120676375
1106	98-99	-0.025565909313506552
1106	100	-0.0021510094033487803
1107	1	-0.031256483917150746
1107	2	-0.0035437865930205703
1107	3	-0.001007832809246878
1107	4	0.06547043766425276
1107	5	0.04762219837784443
1107	6	0.06276583302091154
1107	7	0.03268304048861381
1107	8	0.09752400421622553
1107	9	0.08697998670780294
1107	10-11	0.1731445343550675
1107	12-13	0.1359614529954669
1107	14-15	0.16640121659946772
1107	16-17	0.15886028505426708
1107	18-19	0.1327660121698031
1107	20-21	0.127402519646715
1107	22-23	0.1325972771683368
1107	24-25	0.14518532918041416
1107	26-27	0.1494776220478542
1107	28-29	0.16151335243164056
1107	30-31	0.16278682545655698
1107	32-33	0.14367389393473928
1107	34-35	0.13884314210567794
1107	36-37	0.08001971535689734
1107	38-39	0.10225945852786111
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2216	50-51	-0.5772731629914745
2216	52-53	-0.5277020516214321
2216	54-55	-0.5215800653155824
2216	56-57	-0.5358022548696724
2216	58-59	-0.47850282503198827
2216	60-61	-0.5526376862590112
2216	62-63	-0.5596779211079763
2216	64-65	-0.5446566925153533
2216	66-67	-0.5304128097649823
2216	68-69	-0.5396676611777096
2216	70-71	-0.5935937800929096
2216	72-73	-0.542397060368998
2216	74-75	-0.5310342209275731
2216	76-77	-0.5192609028030901
2216	78-79	-0.5978246079173388
2216	80-81	-0.5607234714383722
2216	82-83	-0.5167541325579421
2216	84-85	-0.5151423113117346
2216	86-87	-0.5006596043653957
2216	88-89	-0.5194795229264386
2216	90-91	-0.4925484220733196
2216	92-93	-0.5001298087790573
2216	94-95	-0.4563279640851654
2216	96-97	-0.4596308190959739
2216	98-99	-0.4772094696484501
2216	100	-0.4394091168419294
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	109.0
1	58.0
2	47.0
3	51.0
4	40.0
5	66.0
6	137.0
7	458.0
8	1395.0
9	2119.0
10	2476.0
11	3734.0
12	5842.0
13	7740.0
14	8981.0
15	9629.0
16	10407.0
17	11420.0
18	12350.0
19	13647.0
20	15122.0
21	17189.0
22	19244.0
23	22112.0
24	26411.0
25	32302.0
26	37182.0
27	36946.0
28	39790.0
29	49153.0
30	58441.0
31	66634.0
32	85030.0
33	114332.0
34	167582.0
35	257063.0
36	455715.0
37	919396.0
38	1209537.0
39	393370.0
40	835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83040696724182	19.075227454917616	13.093639823484088	24.000725754356473
2	14.430046806840046	21.12419066464254	40.20164255423626	24.244119974281155
3	19.150691105440526	26.017307840501825	30.10973041551896	24.72227063853869
4	11.926797299189445	17.293243500785664	40.197917801079704	30.582041398945194
5	12.905303091997583	38.69772577382592	35.56425619387697	12.832714940299528
6	29.53032794755951	38.10096472324221	18.777522932992028	13.591184396206257
7	26.257036064563223	32.86129281525957	23.167742793297883	17.713928326879326
8	24.830324125222145	34.75894856258324	21.337241086440926	19.07348622575369
9	25.670080463377264	16.71781568220308	21.17987348427742	36.432230370142236
10-11	24.078269517000173	26.10863746535755	29.32659035624145	20.486502661400827
12-13	25.73886991504309	24.002054036972666	28.230659844184885	22.02841620379936
14-15	22.879287393696362	25.585727454294055	26.493623240265187	25.041361911744403
16-17	22.00129129368569	28.36543416833102	27.419145859789616	22.214128678193667
18-19	21.835832052068962	29.041148336989746	27.821514302556654	21.301505308384634
20-21	22.958314028420396	27.766477077431457	26.911551006537497	22.363657887610646
22-23	22.461896899673736	27.69312852872598	26.517472947573562	23.327501624026723
24-25	22.93610005184802	27.754550556922585	26.66097788814489	22.64837150308451
26-27	23.08517748362771	27.105682181557512	27.241988810337865	22.567151524476913
28-29	22.354927006966584	28.089625986742593	27.239352222606968	22.316094783683855
30-31	22.822139399025115	27.75063956772903	26.914412662420062	22.512808370825795
32-33	22.549378049284456	27.87839082862748	26.862667642275635	22.70956347981243
34-35	22.970725984499275	27.67540901990318	27.048077432641954	22.30578756295559
36-37	23.1019943474304	27.23709054212618	27.448438930745144	22.212476179698278
38-39	23.123516409567717	27.41638652070876	27.352958623325325	22.1071384463982
40-41	22.653817667928365	27.61957748254259	27.429951875208847	22.296652974320192
42-43	22.537347869701726	27.71574676532414	26.7960723408166	22.95083302415753
44-45	22.821827212278492	27.568677312638368	26.75889328543744	22.8506021896457
46-47	22.865235280129895	27.612050256246167	26.926332205321767	22.596382258302167
48-49	23.01914630218963	27.383908469663513	27.082822476338077	22.51412275180878
50-51	22.386239922388025	27.71498753576515	27.49695916435831	22.401813377488516
52-53	22.392148100132292	28.024218793879363	26.757198616332907	22.82643448965544
54-55	22.19525880122332	27.513238384537154	27.126241696944792	23.16526111729473
56-57	23.043507281487003	27.274102232002118	26.69966104711748	22.982729439393403
58-59	23.1669116267681	27.205148502476977	26.972277341115646	22.65566252963928
60-61	22.824752097148522	27.326437609185643	27.471068122357707	22.37774217130813
62-63	22.633722992741816	27.34422356693365	27.2106264028067	22.811427037517838
64-65	22.189453243964717	27.924020386470506	27.1460144902428	22.740511879321975
66-67	22.730977540652038	27.282663511474286	26.82280686653118	23.16355208134249
68-69	22.442484938557875	27.53510770122612	26.95841300935103	23.063994350864974
70-71	22.76096007703376	27.369076950479425	26.724745941168138	23.145217031318673
72-73	22.777294642704085	26.943545413352822	27.311280571092812	22.967879372850284
74-75	22.59982456044367	27.911278404825257	26.809194588972353	22.67970244575872
76-77	22.7452416942947	26.983726156192084	27.08471047979225	23.186321669720957
78-79	22.66703642545539	27.080118105593286	27.35802077084738	22.894824698103942
80-81	22.664531320803903	27.408583443539435	27.26085842703132	22.666026808625343
82-83	22.631117327360677	27.045771703425565	27.484359342777502	22.838751626436256
84-85	22.609979808707934	27.39718099995526	26.945806902776397	23.047032288560406
86-87	22.642673791876472	27.404217305282803	27.096428374304992	22.85668052853573
88-89	22.870100606214596	26.897935539323104	26.963851423020834	23.268112431441462
90-91	22.81077440422495	26.783240247914392	27.19500037688045	23.210984970980206
92-93	22.835344207127296	27.42965589013383	27.185481831621537	22.549518071117337
94-95	22.307221233442224	27.382039505350903	27.287438406246856	23.023300854960016
96-97	22.178425678094023	27.133839439940242	27.527971864739108	23.159763017226624
98-99	22.6388268368665	27.400665602917712	26.78862700402705	23.17188055618874
100	22.502084275988533	26.83317209421292	26.905188722711426	23.75955490708712
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1472.0
1	1035.5
2	583.0
3	570.0
4	517.5
5	415.0
6	334.5
7	322.5
8	370.5
9	400.5
10	368.5
11	344.0
12	363.5
13	400.0
14	470.0
15	525.5
16	595.5
17	774.5
18	1032.5
19	1427.0
20	2147.0
21	3238.5
22	4842.0
23	7015.5
24	10008.0
25	13754.5
26	18645.0
27	25524.5
28	32530.0
29	39103.5
30	47278.0
31	56341.5
32	65992.5
33	75252.0
34	84256.0
35	93020.5
36	102094.0
37	112233.0
38	116954.5
39	118650.5
40	121405.5
41	123577.5
42	124932.5
43	129142.0
44	136452.0
45	144285.0
46	152238.5
47	171509.0
48	226555.5
49	231133.5
50	193141.5
51	174209.5
52	148638.5
53	135529.5
54	127086.5
55	115087.0
56	104277.5
57	93367.0
58	82105.0
59	72595.5
60	61173.5
61	48447.0
62	38032.5
63	29814.5
64	21958.0
65	15681.5
66	11530.0
67	8591.5
68	6575.5
69	5176.5
70	4150.5
71	3253.0
72	2624.5
73	2320.0
74	2000.5
75	1588.5
76	1080.0
77	747.0
78	521.5
79	311.0
80	202.0
81	139.0
82	85.0
83	66.5
84	59.0
85	46.0
86	37.0
87	27.0
88	20.5
89	16.0
90	14.5
91	9.5
92	8.0
93	9.0
94	5.5
95	2.5
96	3.0
97	6.5
98	7.0
99	5.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060961203590002365
2	0.009163625898497165
3	0.011302615498146372
4	0.008847638798548987
5	0.01149706909811448
6	0.011594295898098536
7	0.0026494302995654934
8	0.005323167299127
9	0.007170476498824041
10-11	0.00683018269887985
12-13	0.005882221399035315
14-15	0.009819906798389535
16-17	0.009430999598453317
18-19	0.007705223898736344
20-21	0.006951716198859919
22-23	0.0021754496496432262
24-25	0.0035244714994219868
26-27	0.004995026849180816
28-29	0.005845761349041295
30-31	0.009139319198501151
32-33	0.010257427398317781
34-35	0.01161860259809455
36-37	0.01802341804704416
38-39	0.018473091996970413
40-41	0.017585897447115913
42-43	0.024452540195989782
44-45	0.027794711445441666
46-47	0.03038337499501713
48-49	0.031926850444763996
50-51	0.032194224144720146
52-53	0.030456295095005168
54-55	0.03705556414392289
56-57	0.03824659244372756
58-59	0.036885417243950794
60-61	0.03751739144384715
62-63	0.032935578494598564
64-65	0.03554854874417004
66-67	0.03660589019399663
68-69	0.03898794679360598
70-71	0.03912163364358405
72-73	0.03610760284407835
74-75	0.038027832143763436
76-77	0.03822228574373155
78-79	0.03648435669401657
80-81	0.041843984043137585
82-83	0.038234439093729554
84-85	0.03470996759430757
86-87	0.033324485694534785
88-89	0.03097888914491946
90-91	0.033591859394490935
92-93	0.03529332839421189
94-95	0.03793060534377938
96-97	0.03373769959446702
98-99	0.031100422644899532
100	0.028025625095403798
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4114092.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.988253916489434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.13710373235833	45.95629610794545
2	14.31594872793768	17.748413296056896
3	5.213332983189899	9.694962261395542
4	2.4974549504384402	6.192514864510863
5	1.3432267024176772	4.163213894843787
6	0.7800519802019371	2.9012436130090844
7	0.4813294276003342	2.088573954289762
8	0.32985756541964645	1.6357835617206453
9	0.20871702144834967	1.1644203350010303
>10	0.6809903692785946	6.292938267827288
>50	0.008955152130392776	0.36658135323800606
>100	0.0026086746904551102	0.2741104978491965
>500	6.038755546623845E-5	0.029534787869718127
>1k	2.4155022186495085E-4	0.4006100123976166
>5k	6.038755546623771E-5	0.35752440930757207
>10k+	6.038755546623771E-5	0.7332787827375175
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	19589	0.4761439462219124	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	9551	0.23215329166192686	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	4579	0.11130037928174673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.722679998405481E-5
2	0.0	0.0	0.0	7.292009998804111E-5	9.722679998405481E-5
3	0.0	0.0	0.0	7.292009998804111E-5	9.722679998405481E-5
4	0.0	0.0	0.0	9.722679998405481E-5	9.722679998405481E-5
5	0.0	0.0	0.0	9.722679998405481E-5	9.722679998405481E-5
6	0.0	0.0	0.0	9.722679998405481E-5	1.215334999800685E-4
7	0.0	0.0	0.0	1.4584019997608222E-4	1.215334999800685E-4
8	0.0	0.0	0.0	2.673736999561507E-4	1.215334999800685E-4
9	0.0	0.0	0.0	3.402937999441918E-4	1.215334999800685E-4
10-11	0.0	0.0	0.0	4.6182729992426034E-4	1.215334999800685E-4
12-13	0.0	0.0	0.0	5.104406999162877E-4	5.469007499103082E-4
14-15	0.0	0.0	0.0	5.712074499063221E-4	6.5628089989237E-4
16-17	0.0	0.0	0.0	5.833607999043289E-4	8.14274449866459E-4
18-19	0.0	0.0	0.0	6.319741998963562E-4	9.236545998485207E-4
20-21	0.0	0.0	0.0	6.927409498863905E-4	0.0010330347498305823
22-23	0.0	0.0	0.0	9.115012498505139E-4	0.0010938014998206165
24-25	0.0	0.0	0.0	0.001130261549814637	0.0010938014998206165
26-27	0.0	0.0	0.0	0.0015070153997528493	0.0011545682498106508
28-29	0.0	0.0	0.0	0.003889071999362192	0.0011910282998046713
30-31	0.0	0.0	0.0	0.010014360398357645	0.0012031816498026782
32-33	0.0	0.0	0.0	0.024027172946059543	0.0012153349998006852
34-35	0.0	0.0	0.0	0.04753175184220479	0.0013125617997847398
36-37	0.0	0.0	0.0	0.08357858793629311	0.0013125617997847398
38-39	0.0	0.0	0.0	0.13243505492828064	0.0014340952997648083
40-41	0.0	0.0	0.0	0.18580041476952874	0.0014827086997568357
42-43	0.0	0.0	0.0	0.23649203761121532	0.0015191687497508564
44-45	0.0	0.0	0.0	0.2957274655015007	0.001628548899732918
46-47	0.0	0.0	0.0	0.36898785928948596	0.0017500823997129865
48-49	0.0	0.0	0.0	0.4433299012272939	0.0018351558496990344
50-51	0.0	0.0	0.0	0.5200661531147092	0.001871615899693055
52-53	0.0	0.0	0.0	0.5943231216025311	0.00196884269967711
54-55	0.0	0.0	0.0	0.6744987715393822	0.0020053027496711303
56-57	0.0	0.0	0.0	0.7706196166736183	0.0020660694996611642
58-59	0.0	0.0	0.0	0.8756974807563855	0.002114682899653192
60-61	0.0	0.0	0.0	0.986548186088206	0.0021632962996452195
62-63	0.0	0.0	0.0	1.092829231820776	0.002284829799625288
64-65	0.0	0.0	0.0	1.20286566270273	0.0022969831496232945
66-67	1.2153349998006851E-5	0.0	0.0	1.3269635195323781	0.0023212898496193088
68-69	2.4306699996013703E-5	0.0	0.0	1.4689024941591	0.002357749899613329
70-71	2.4306699996013703E-5	0.0	0.0	1.6212568897341137	0.0023699032496113355
72-73	2.4306699996013703E-5	0.0	0.0	1.7694183795598155	0.002540050149583432
74-75	2.4306699996013703E-5	0.0	0.0	1.9138366375861309	0.0027466570995495483
76-77	2.4306699996013703E-5	0.0	0.0	2.0695575111105926	0.002843883899533603
78-79	2.4306699996013703E-5	0.0	0.0	2.2433382627320926	0.0029532640495156647
80-81	2.4306699996013703E-5	0.0	0.0	2.427655968801864	0.003038337499501713
82-83	2.4306699996013703E-5	0.0	0.0	2.613930364221315	0.003257097799465836
84-85	2.4306699996013703E-5	0.0	0.0	2.804288285240097	0.003257097799465836
86-87	2.4306699996013703E-5	0.0	0.0	3.0099837339563624	0.0033664779494478976
88	2.4306699996013703E-5	0.0	0.0	3.169034625380278	0.003475858099429959
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1485	0.0	21.531933	1
TGATCGC	955	0.0	20.173933	9
GGCGTCG	945	0.0	18.895653	8
CATGGGG	6875	0.0	18.797344	4
TACGGGC	965	0.0	18.505157	4
GTATAAG	2410	0.0	17.950287	1
CTAGTAC	1820	0.0	17.816599	3
TCTAACG	585	0.0	17.672932	2
CGGGCGT	925	0.0	17.272816	6
TAGTACT	2050	0.0	16.963459	4
CGTTAAC	310	1.2551754E-7	16.685205	1
GCATCGT	1415	0.0	16.60442	8
CTAACGC	595	0.0	16.586294	3
TACCTGG	4140	0.0	16.57274	2
GTACATG	23155	0.0	16.489664	1
TCGACGG	290	1.1497268E-6	16.208944	94
GTACCTG	4230	0.0	16.118633	1
ATACGGG	1025	0.0	16.046904	3
GTGATCG	1210	0.0	15.922403	8
TACATGG	23235	0.0	15.917462	2
>>END_MODULE