FastQCFastQC Report
Mon 16 Jan 2017
ERR1146567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4114092
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT210800.5123852359159689No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101670.24712621885947128No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA99290.24134122426042004No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA98370.23910500786078676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95350.23176438446199066No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA76800.18667545596938523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50350.12238423447992898No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG45990.11178651328166703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT122450.021.56908612-13
GTACTAG13500.021.2659171
TACGGGC9850.019.5729054
AGTACTT132600.019.54592512-13
GTACTTT138450.019.0764514-15
GTACCTG39450.018.6108261
CGGGCGT10400.018.5287866
ATACGGG10650.018.1026383
CATGGGG69600.018.0388854
TCTAACG5300.017.7595372
CTAGTAC18600.017.6967183
GGCGTCG11100.017.3586168
CTAACGC5750.017.1735123
TACCTGG41550.016.53712
CTGTGCG18050.016.4027849
GTATAAG21150.016.2442841
TGCGACG6550.016.14400522-23
TAGTACT21950.016.0669844
ACTTTTT165650.016.02946916-17
TACTTTT161000.015.937530514-15
Produced by FastQC (version 0.11.5)