FastQCFastQC Report
Mon 16 Jan 2017
ERR1146566_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146566_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3422164
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA748012.1857806931520525No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA603921.7647313220523622No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT485501.4186929673738606No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA170310.4976675577207872No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG135560.3961236223629259No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC111870.3268984186614084No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC84810.24782564482590547No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG78080.22815972583429667No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA77330.22596813010714858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13600.026.2749861
TACCCCG2704.7293724E-1122.6269075
TAGGCAT18050.022.3906575
GCATCGT14850.021.5180388
TAGTACT19300.020.9404854
TATTCGC2701.1477823E-920.8851559
GGGCACC17800.020.86161494
GCTATTG18450.020.3757618
CTATTGA17800.020.3278269
CTAGTAC20350.020.0906523
CTAGGCA21200.019.7287564
GGGCTAT19400.019.3782626
TACCTGG33900.019.2687852
TTTAGGG19600.019.1810842
CCGTATC2953.441528E-919.12053594
GTACCCG6150.019.1131671
ATACGGG8400.019.0212423
CTAACGC5500.018.797463
CATGGGG59000.018.7180864
TACGGGC8550.018.6878094
Produced by FastQC (version 0.11.5)