##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146566_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3422164
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.699836419294925	33.0	31.0	34.0	30.0	34.0
2	31.867290988976567	33.0	31.0	34.0	30.0	34.0
3	31.935949592129425	34.0	31.0	34.0	30.0	34.0
4	35.382974632425565	37.0	35.0	37.0	33.0	37.0
5	35.45678816094144	37.0	35.0	37.0	33.0	37.0
6	35.47006338679268	37.0	35.0	37.0	33.0	37.0
7	35.4880441147765	37.0	35.0	37.0	33.0	37.0
8	35.46424952164771	37.0	35.0	37.0	33.0	37.0
9	37.11406934325766	39.0	38.0	39.0	34.0	39.0
10-11	37.096674501864904	39.0	38.0	39.0	34.0	39.0
12-13	37.0785828791373	39.0	38.0	39.0	34.0	39.0
14-15	38.42692737694628	40.0	39.0	41.0	34.0	41.0
16-17	38.43669429635751	40.0	39.0	41.0	34.0	41.0
18-19	38.52275811445623	40.5	39.0	41.0	34.0	41.0
20-21	38.592537207451194	41.0	39.0	41.0	34.0	41.0
22-23	38.58210871834313	41.0	39.0	41.0	34.0	41.0
24-25	38.584965828639426	41.0	39.0	41.0	34.0	41.0
26-27	38.50895807448153	40.5	38.5	41.0	34.0	41.0
28-29	38.41740167917143	40.0	38.0	41.0	34.0	41.0
30-31	38.38158676790475	40.0	38.0	41.0	34.0	41.0
32-33	38.35225269741602	40.0	38.0	41.0	33.0	41.0
34-35	38.23696526525321	40.0	38.0	41.0	33.0	41.0
36-37	38.045201954085194	40.0	38.0	41.0	33.0	41.0
38-39	37.93895397765858	40.0	38.0	41.0	32.5	41.0
40-41	37.88302080204222	40.0	38.0	41.0	32.0	41.0
42-43	37.775654965688375	40.0	38.0	41.0	32.0	41.0
44-45	37.65219390420798	40.0	37.0	41.0	32.0	41.0
46-47	37.485099778970266	40.0	37.0	41.0	31.0	41.0
48-49	37.30187229484034	40.0	37.0	41.0	31.0	41.0
50-51	37.084950925788476	40.0	36.0	41.0	30.0	41.0
52-53	36.8739293324341	39.0	36.0	41.0	30.0	41.0
54-55	36.68059771536373	39.0	35.0	41.0	30.0	41.0
56-57	36.41850112969455	39.0	35.0	41.0	29.0	41.0
58-59	36.179363116437436	38.5	35.0	40.0	28.5	41.0
60-61	35.866692829449434	38.0	35.0	40.0	28.0	41.0
62-63	35.54473675136551	37.5	34.0	40.0	28.0	41.0
64-65	35.1861474201704	37.0	34.0	40.0	28.0	41.0
66-67	34.83042031299493	36.0	34.0	39.0	27.5	41.0
68-69	34.55122548188807	36.0	34.0	39.0	27.0	41.0
70-71	34.17966730992437	35.5	33.0	38.5	26.5	40.5
72-73	33.77112318404378	35.0	33.0	37.0	26.0	40.0
74-75	33.44412745853209	35.0	33.0	37.0	26.0	39.0
76-77	33.07803673348209	35.0	32.0	36.5	26.0	39.0
78-79	32.79952027430596	35.0	32.0	36.0	25.5	39.0
80-81	32.46775826640687	35.0	32.0	36.0	25.0	37.0
82-83	32.268812803828226	35.0	32.0	35.0	25.0	37.0
84-85	32.10497188328789	35.0	32.0	35.0	25.0	37.0
86-87	31.88676916126755	35.0	31.0	35.0	24.5	36.0
88-89	31.639984378305655	35.0	31.0	35.0	23.5	36.0
90-91	31.393984332720464	34.5	31.0	35.0	23.0	36.0
92-93	31.32376224517586	34.0	31.0	35.0	23.0	35.5
94-95	31.359820569674625	34.5	31.0	35.0	23.0	35.0
96-97	31.289778485192407	34.5	31.0	35.0	23.0	35.0
98-99	31.16931026683701	34.0	31.0	35.0	21.0	35.0
100	29.821426442449866	34.0	29.0	35.0	18.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.14753469318629442
1101	2	0.21815576370223866
1101	3	0.2444046652423708
1101	4	0.2502905789173866
1101	5	0.10998816303160197
1101	6	0.1702948189253206
1101	7	0.1786864443882905
1101	8	0.23030017861569263
1101	9	0.2929104248694756
1101	10-11	0.354862961016984
1101	12-13	0.3187544292821727
1101	14-15	0.38175808105091846
1101	16-17	0.3899552766302392
1101	18-19	0.3488528210102828
1101	20-21	0.308116954312581
1101	22-23	0.29625884041854533
1101	24-25	0.35777473897361745
1101	26-27	0.33264215800001296
1101	28-29	0.35844280529329353
1101	30-31	0.36380248540556437
1101	32-33	0.3740210194534441
1101	34-35	0.2898583217394801
1101	36-37	0.34408799652430133
1101	38-39	0.3635471722610717
1101	40-41	0.3451585673554831
1101	42-43	0.31313864375660216
1101	44-45	0.2822386291302337
1101	46-47	0.27448337658583455
1101	48-49	0.29761877968698514
1101	50-51	0.22991163576871543
1101	52-53	0.31808489514634886
1101	54-55	0.40342183465188697
1101	56-57	0.26999722837704354
1101	58-59	0.2838535783943854
1101	60-61	0.33472585643617947
1101	62-63	0.3552771401573054
1101	64-65	0.3049627321624939
1101	66-67	0.2770059189101346
1101	68-69	0.12246939615645402
1101	70-71	0.1985000220348354
1101	72-73	0.25729010681416753
1101	74-75	0.26731415565126326
1101	76-77	0.27116128768019365
1101	78-79	0.31066612607366295
1101	80-81	0.25915798534710177
1101	82-83	0.2560860369271438
1101	84-85	0.2508303977912192
1101	86-87	0.33024555228425356
1101	88-89	0.28493589353893256
1101	90-91	0.21336963881658733
1101	92-93	0.26160086406891736
1101	94-95	0.2192553503003225
1101	96-97	0.21372672555779104
1101	98-99	0.10882700891396269
1101	100	0.21468602275667692
1102	1	0.2444133493976004
1102	2	0.30785780745420865
1102	3	0.3388962055045823
1102	4	0.31911108234429264
1102	5	0.3683664147306871
1102	6	0.3421927579333186
1102	7	0.31117250196101764
1102	8	0.32236464556196864
1102	9	0.4544872413358618
1102	10-11	0.42564204885764667
1102	12-13	0.36140308191078674
1102	14-15	0.4826378614560056
1102	16-17	0.438333321808102
1102	18-19	0.43103322717466597
1102	20-21	0.42617638214104403
1102	22-23	0.4778922347205281
1102	24-25	0.4764874024059793
1102	26-27	0.4843007927869891
1102	28-29	0.5228554724071159
1102	30-31	0.5337685530708995
1102	32-33	0.4758824491010927
1102	34-35	0.4564392372031634
1102	36-37	0.5454250867480326
1102	38-39	0.48319905829368537
1102	40-41	0.46286219503543435
1102	42-43	0.4926976221769337
1102	44-45	0.4494826572997326
1102	46-47	0.49290560832599084
1102	48-49	0.5256819608141754
1102	50-51	0.5060235505167512
1102	52-53	0.43159344826765533
1102	54-55	0.48295674886028195
1102	56-57	0.4844495385862828
1102	58-59	0.48005973865305407
1102	60-61	0.5151147358969297
1102	62-63	0.5630029194831039
1102	64-65	0.4922323599843139
1102	66-67	0.5077096534423617
1102	68-69	0.5511944329037632
1102	70-71	0.514322376071469
1102	72-73	0.455892137725904
1102	74-75	0.49712378798062673
1102	76-77	0.464983250224428
1102	78-79	0.4861366577401043
1102	80-81	0.4431430278231261
1102	82-83	0.43453182106577515
1102	84-85	0.485029279523701
1102	86-87	0.45573839722182896
1102	88-89	0.4599327732726515
1102	90-91	0.5561315638457955
1102	92-93	0.5652804436994607
1102	94-95	0.5020215135041681
1102	96-97	0.46238658241164643
1102	98-99	0.4398241831648946
1102	100	0.35489821882253736
1103	1	0.2360218416821489
1103	2	0.22082354528949466
1103	3	0.2653097254660395
1103	4	0.24069338636321902
1103	5	0.24785906360422416
1103	6	0.17478431892631363
1103	7	0.21102687013238608
1103	8	0.29744926284504913
1103	9	0.36241041589426715
1103	10-11	0.3976337281581408
1103	12-13	0.34791969980499005
1103	14-15	0.4791177237178772
1103	16-17	0.4071777082740411
1103	18-19	0.3719631652395208
1103	20-21	0.39031016167385246
1103	22-23	0.4078286346468829
1103	24-25	0.3925953893373162
1103	26-27	0.4163662869558422
1103	28-29	0.45494603836355196
1103	30-31	0.44507162005565704
1103	32-33	0.47438818035503516
1103	34-35	0.47480681899008204
1103	36-37	0.5178225666416338
1103	38-39	0.5319980609066377
1103	40-41	0.48735963301214014
1103	42-43	0.5129134158361168
1103	44-45	0.472871501137746
1103	46-47	0.5079451551534291
1103	48-49	0.5074866496302022
1103	50-51	0.5128224610692342
1103	52-53	0.47446948590667404
1103	54-55	0.5052084500202412
1103	56-57	0.5595821448377762
1103	58-59	0.5984406887954634
1103	60-61	0.5994334590377974
1103	62-63	0.5649213029795987
1103	64-65	0.5507564249829215
1103	66-67	0.5387302612765126
1103	68-69	0.4697088707276933
1103	70-71	0.39938904156289823
1103	72-73	0.4620756142426501
1103	74-75	0.484187675988089
1103	76-77	0.5177077014852713
1103	78-79	0.4437328707249293
1103	80-81	0.47037094016589265
1103	82-83	0.6136193549028945
1103	84-85	0.5504512200800562
1103	86-87	0.5435077720333013
1103	88-89	0.5471457698605313
1103	90-91	0.5481605961923179
1103	92-93	0.5473815378479401
1103	94-95	0.5676550952048949
1103	96-97	0.457605656949287
1103	98-99	0.3811228527931654
1103	100	0.5331136755795818
1104	1	0.02908572937415599
1104	2	0.10896880250853513
1104	3	0.11626446861527029
1104	4	0.04455636737147728
1104	5	0.01967213649614763
1104	6	-4.7104920717799814E-5
1104	7	-0.06331539746085468
1104	8	0.05146903532708791
1104	9	0.10992541373988018
1104	10-11	0.15981687680345402
1104	12-13	0.1206490775371023
1104	14-15	0.15998133815263316
1104	16-17	0.19424973107739874
1104	18-19	0.10289716551204009
1104	20-21	0.1248643601499424
1104	22-23	0.14612027967761776
1104	24-25	0.09329275693460204
1104	26-27	0.10972502179686927
1104	28-29	0.13658988142108797
1104	30-31	0.06767205682533017
1104	32-33	0.004890014345733107
1104	34-35	0.10554278141168538
1104	36-37	0.11379206826018873
1104	38-39	0.18519548374460015
1104	40-41	0.19276074482296224
1104	42-43	0.12213078424360191
1104	44-45	0.07293889477181636
1104	46-47	0.0607250504907455
1104	48-49	0.08238937999306017
1104	50-51	0.10730838206032445
1104	52-53	0.06083926151901409
1104	54-55	0.13915467873876963
1104	56-57	0.1274605159004949
1104	58-59	0.1661381466191969
1104	60-61	0.19132582993110248
1104	62-63	0.18652298818872026
1104	64-65	0.18098463345390314
1104	66-67	0.13311398700284371
1104	68-69	0.10907568359802866
1104	70-71	0.10841127225535274
1104	72-73	0.1869993360652984
1104	74-75	0.18847697697248122
1104	76-77	0.18331259617810503
1104	78-79	0.2278601507841529
1104	80-81	0.2151163082345704
1104	82-83	0.26047731085029113
1104	84-85	0.24623041754340136
1104	86-87	0.24492497177256922
1104	88-89	0.21049357625298626
1104	90-91	0.27437055130005206
1104	92-93	0.27022429708326
1104	94-95	0.19679589081804494
1104	96-97	0.29014172911571734
1104	98-99	0.20875428879315194
1104	100	0.16094825919372013
1105	1	0.1491621132251879
1105	2	0.15853813145981732
1105	3	0.10935343369123984
1105	4	0.1280920348878638
1105	5	0.10097310947804061
1105	6	0.17166311559203962
1105	7	0.10108231292225156
1105	8	0.18220194505747145
1105	9	0.10972113777003756
1105	10-11	0.17225410746684844
1105	12-13	0.20563310224146392
1105	14-15	0.3054343293931936
1105	16-17	0.20914362408028353
1105	18-19	0.16527309612414598
1105	20-21	0.1722302789093817
1105	22-23	0.21358369166798497
1105	24-25	0.22427490578662912
1105	26-27	0.21444950986852263
1105	28-29	0.2936979516077898
1105	30-31	0.3171901916467803
1105	32-33	0.27832492125129704
1105	34-35	0.26476843481430734
1105	36-37	0.25653346213170636
1105	38-39	0.286779886829855
1105	40-41	0.1936211721230734
1105	42-43	0.26085582947860786
1105	44-45	0.19140950725279282
1105	46-47	0.2092619443533721
1105	48-49	0.18922269166468197
1105	50-51	0.17112589518205823
1105	52-53	0.18170811013265364
1105	54-55	0.2906732077860923
1105	56-57	0.25790573913089787
1105	58-59	0.269451689261615
1105	60-61	0.2320030699288793
1105	62-63	0.19230739841974298
1105	64-65	0.21493530827227403
1105	66-67	0.1463240056560693
1105	68-69	0.181774972627899
1105	70-71	0.2358490992630351
1105	72-73	0.21704844342159646
1105	74-75	0.3160523687862167
1105	76-77	0.30888764554696024
1105	78-79	0.2763486380597513
1105	80-81	0.3066156317160562
1105	82-83	0.23301777035126747
1105	84-85	0.18287043253917545
1105	86-87	0.1656246001404682
1105	88-89	0.1256974302078362
1105	90-91	0.1326820373745079
1105	92-93	0.18478183809423143
1105	94-95	0.1418796577739485
1105	96-97	0.10440503333260409
1105	98-99	0.08713608308353926
1105	100	-0.027485146926412796
1106	1	0.11409550613569408
1106	2	0.10507348170249031
1106	3	0.13870404385715318
1106	4	0.11107455759207596
1106	5	0.12829399874547676
1106	6	0.15303536920828975
1106	7	0.09909115002047031
1106	8	0.1720734416955736
1106	9	0.21031664917526882
1106	10-11	0.21063310544398917
1106	12-13	0.20977220864138957
1106	14-15	0.26230992312673607
1106	16-17	0.24706298853926256
1106	18-19	0.21098859565779549
1106	20-21	0.1854655990209153
1106	22-23	0.2359895667885823
1106	24-25	0.19461120715395452
1106	26-27	0.16470611785824474
1106	28-29	0.24812918210527357
1106	30-31	0.2580854688676766
1106	32-33	0.22876570739047963
1106	34-35	0.19864087443794887
1106	36-37	0.2156126988149154
1106	38-39	0.24134337783305426
1106	40-41	0.2754862939486671
1106	42-43	0.16275006300276829
1106	44-45	0.18230342795568788
1106	46-47	0.22826756321538255
1106	48-49	0.2694422132742176
1106	50-51	0.20496080447416176
1106	52-53	0.1483364311867561
1106	54-55	0.23394210377363578
1106	56-57	0.16902569272975398
1106	58-59	0.24365324481780704
1106	60-61	0.24539930185071057
1106	62-63	0.27535834307010987
1106	64-65	0.31846266615748675
1106	66-67	0.2521426252222483
1106	68-69	0.2821291677009512
1106	70-71	0.215234156765014
1106	72-73	0.2043349922301445
1106	74-75	0.24154041724597164
1106	76-77	0.24776341686104075
1106	78-79	0.2268188359757346
1106	80-81	0.23689687489492428
1106	82-83	0.2448062612414219
1106	84-85	0.335047047895209
1106	86-87	0.19951060494418726
1106	88-89	0.18742002720812323
1106	90-91	0.21904892863397052
1106	92-93	0.28739048388230515
1106	94-95	0.26667136032325445
1106	96-97	0.1789023714743152
1106	98-99	0.1644112001448761
1106	100	0.10835179796483985
1107	1	0.10905549062244191
1107	2	0.11946509021104745
1107	3	0.1527763485189766
1107	4	0.04891487062850075
1107	5	0.14931413850013797
1107	6	0.0427124060494819
1107	7	0.08670507547483197
1107	8	0.09073911874197194
1107	9	0.08324020857498482
1107	10-11	0.1675162555263512
1107	12-13	0.15171564977246277
1107	14-15	0.1698545764895414
1107	16-17	0.08349687544143336
1107	18-19	0.13538509784919484
1107	20-21	0.13247955656891008
1107	22-23	0.14711307912293137
1107	24-25	0.15615056307521513
1107	26-27	0.17791022186234784
1107	28-29	0.09695197066034922
1107	30-31	0.15393095511220878
1107	32-33	0.17768200948639645
1107	34-35	0.14113952359831927
1107	36-37	0.14263683793088688
1107	38-39	0.13716513230396998
1107	40-41	0.11112184159716776
1107	42-43	0.13830611717332886
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2216	72-73	-1.1887115188067767
2216	74-75	-1.306244234661463
2216	76-77	-1.3263257732721243
2216	78-79	-1.3269676137052286
2216	80-81	-1.2530660733591006
2216	82-83	-1.2478116533767434
2216	84-85	-1.1540058144474656
2216	86-87	-1.1996367232464031
2216	88-89	-1.290439124150577
2216	90-91	-1.3336280223374004
2216	92-93	-1.3330267449579551
2216	94-95	-1.3387880765989486
2216	96-97	-1.2633457496395977
2216	98-99	-1.2748992130534518
2216	100	-1.3920335531949384
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	88.0
1	50.0
2	42.0
3	36.0
4	32.0
5	60.0
6	105.0
7	602.0
8	1828.0
9	2538.0
10	2630.0
11	3323.0
12	4412.0
13	5268.0
14	6212.0
15	6958.0
16	7373.0
17	7967.0
18	9008.0
19	10265.0
20	11893.0
21	13760.0
22	15751.0
23	19099.0
24	23596.0
25	29367.0
26	34136.0
27	35970.0
28	41260.0
29	50381.0
30	60365.0
31	71932.0
32	92086.0
33	125614.0
34	186600.0
35	286742.0
36	481134.0
37	804483.0
38	750637.0
39	218180.0
40	381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.17584089677723	17.22561737789501	12.241682843240882	22.356858882086883
2	13.310472285021591	22.328536292839626	38.54636931328014	25.814622108858646
3	23.254867888201424	26.27915859699143	29.92654951646243	20.53942399834471
4	11.677227776590954	15.441382113844899	41.2955812253732	31.585808884190946
5	12.759216472019904	40.68293629524507	34.18170914682938	12.37613808590565
6	27.559902411994862	37.07619416131947	19.106902586175657	16.25700084051001
7	25.95401600205724	34.508190972688965	21.55996680362121	17.977826221632583
8	23.04785978857239	31.18678540901088	21.1903652130714	24.574989589345332
9	24.536282097589996	20.10720334971016	22.369654590363037	32.9868599623368
10-11	24.35200908962592	23.91129198406861	28.26986463459289	23.466834291712583
12-13	30.686211594401108	21.765437488785675	25.682503195535194	21.86584772127803
14-15	26.04140045253662	23.78450372571926	27.76207708026936	22.412018741474753
16-17	21.511093719588782	26.86643408265597	29.686856158927082	21.935616038828165
18-19	20.553071722872655	31.69216755452442	27.438384503144007	20.316376219458913
20-21	22.468058544249004	28.50039502624912	24.065682921993165	24.96586350750871
22-23	21.397276742377	27.75557716685593	23.415436468718713	27.431709622048356
24-25	24.18909692146497	27.547436341578774	24.55987061494284	23.70359612201342
26-27	25.691159332367818	24.042841563013955	27.414637738588265	22.851361366029963
28-29	22.140366816576076	27.168485972421326	28.217038474053798	22.4741087369488
30-31	22.425040233860503	28.935444343427523	26.011004512446267	22.628510910265707
32-33	22.394845187286734	28.396139238508724	25.18963636809971	24.019379206104833
34-35	24.09966769932524	26.03345860547109	25.63725788890803	24.229615806295637
36-37	25.408970409459226	25.09608585233094	27.793635602816796	21.701308135393045
38-39	26.211475278315593	24.29823232098127	27.973776570971882	21.516515829731254
40-41	22.61955473552183	26.484949245800028	29.21363321978308	21.681862798895057
42-43	21.79468424545435	28.5643657178566	25.89934208151502	23.741607955174032
44-45	22.493797126239315	28.2733394588706	24.550314487928418	24.682548926961665
46-47	23.261177723022833	27.233640977913108	24.087885144594512	25.417296154469547
48-49	24.32606078551935	26.530844300811314	25.3572173432135	23.78587757045584
50-51	24.07216324583483	27.34680210660531	26.945609373031488	21.63542527452837
52-53	22.282646345687564	27.985995711899257	26.920108912497316	22.81124902991586
54-55	20.700679767007184	26.592690531495133	28.009066678203666	24.69756302329402
56-57	23.886761223301985	27.468182215483072	24.923227180019218	23.721829381195725
58-59	23.940538246209755	26.186724031888797	25.68493911522388	24.187798606677564
60-61	23.223896914810897	25.25505282297434	28.081008869114783	23.440041393099982
62-63	25.742724175034294	24.945111415568626	26.30507091304029	23.007093496356788
64-65	23.471866631062486	27.33031633204436	27.399449245990837	21.79836779090231
66-67	22.644499138301356	25.920985647028182	27.398932928990504	24.035582285679958
68-69	21.56732829881346	28.599884880157557	26.46652827729792	23.36625854373107
70-71	22.926738360684492	27.842390092924507	24.2963937397456	24.9344778066454
72-73	22.791451855409473	25.91609970331163	25.519570046678837	25.772878394600063
74-75	22.893628577513145	28.276957387089887	25.59472782844044	23.23468620695653
76-77	24.48776843001011	24.90276877860865	26.429747309177383	24.17971548220386
78-79	23.099330798401283	25.374689314917926	28.293437264085792	23.232542622595002
80-81	23.042165086500937	27.98567713985983	26.82009205464811	22.15206571899112
82-83	22.564150385769064	26.580194449941814	26.81390972772974	24.041745436559385
84-85	23.010674454708912	26.12110713595643	26.11892939168441	24.749289017650252
86-87	24.109336371429137	26.452523785060084	26.743067273408894	22.695072570101892
88-89	25.023241864908822	24.384702592551292	26.670816416816923	23.92123912572296
90-91	23.156362101462495	24.88786930635649	28.208893402178536	23.746875190002477
92-93	22.908601464536204	27.957978880990975	26.788317209871593	22.345102444601224
94-95	21.982394031153564	28.183464912081966	25.93579303961014	23.898348017154326
96-97	21.180228530337708	26.55975317086574	25.893177121242683	26.36684117755387
98-99	23.228245876237583	27.07779720305271	24.915735846530648	24.778221074179054
100	21.992064130013812	27.667248825330475	26.071481289323113	24.2692057553326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1511.0
1	1009.0
2	525.0
3	530.5
4	464.0
5	355.5
6	295.5
7	272.0
8	296.5
9	337.5
10	298.5
11	250.0
12	243.0
13	253.5
14	292.0
15	349.0
16	400.0
17	517.5
18	705.5
19	940.5
20	1284.0
21	1891.0
22	2904.0
23	4154.5
24	5783.0
25	7890.5
26	10578.5
27	13857.5
28	16965.0
29	20477.0
30	25278.0
31	30037.5
32	35938.5
33	41801.5
34	46337.0
35	50879.5
36	56685.5
37	64087.0
38	66079.5
39	64583.0
40	65177.0
41	66628.5
42	69686.0
43	74386.0
44	83076.0
45	97231.5
46	118363.5
47	183627.0
48	427111.0
49	438878.0
50	227426.0
51	164765.5
52	119143.0
53	101416.5
54	92542.0
55	80833.5
56	73094.0
57	64862.5
58	56508.0
59	51665.0
60	43730.5
61	34282.0
62	26995.0
63	21610.5
64	16203.0
65	10967.0
66	7611.0
67	5361.0
68	4006.5
69	3253.0
70	2770.5
71	2307.5
72	1899.0
73	1848.0
74	1636.0
75	1238.5
76	936.0
77	697.5
78	514.0
79	342.0
80	235.5
81	164.0
82	120.5
83	92.5
84	65.5
85	47.0
86	41.0
87	35.5
88	25.0
89	22.0
90	18.0
91	15.0
92	12.5
93	7.0
94	7.5
95	6.5
96	7.5
97	8.5
98	5.0
99	4.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06121857397833651
2	0.009701463752175524
3	0.011776174373875712
4	0.009730685028537498
5	0.011980723308409533
6	0.012623591388372973
7	0.0030390127416453448
8	0.0055228212324131744
9	0.007480646748665464
10-11	0.007042327603235847
12-13	0.005902697825118843
14-15	0.009979065877614282
16-17	0.00929236588310788
18-19	0.007509868025027438
20-21	0.006998495688692885
22-23	0.0025130297671298045
24-25	0.003813376565237668
26-27	0.0046754042179159155
28-29	0.006092636121471677
30-31	0.008620276526782468
32-33	0.010285889279415013
34-35	0.012082997775676443
36-37	0.01795647432443331
38-39	0.01880389133893057
40-41	0.018248687088053057
42-43	0.024648146611325465
44-45	0.0282715848802103
46-47	0.031047606134597876
48-49	0.032216457189076855
50-51	0.03258172314360153
52-53	0.031237544430950708
54-55	0.03757856140149917
56-57	0.039653272023199355
58-59	0.03728634863787943
60-61	0.03810454437601471
62-63	0.033399918881736816
64-65	0.03557690397070392
66-67	0.038119155014195695
68-69	0.04035458265588674
70-71	0.04022308691225786
72-73	0.03607366566885748
74-75	0.03899579330505493
76-77	0.03867435926507321
78-79	0.0378853848032999
80-81	0.04320365710117925
82-83	0.03901040394323592
84-85	0.03486098269983554
86-87	0.03394051249443335
88-89	0.031398261450941566
90-91	0.03377979547444249
92-93	0.03557690397070392
94-95	0.039025014581416904
96-97	0.03430577844895803
98-99	0.0314713146418465
100	0.028899842321992754
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3422164.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.95717799066506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77847773599609	40.54656932817456
2	13.900535842082304	15.278684448778726
3	5.4590234939278535	9.00037577433046
4	2.6372210810046317	5.797369133980227
5	1.4325673939987786	3.9364930627807033
6	0.8246420212546509	2.719199900440419
7	0.5435366585845514	2.090986862319778
8	0.35629332192664587	1.5664700408006402
9	0.24763322616339042	1.2248300957997655
>10	0.8082152133938613	6.525167624069152
>50	0.008121329516082403	0.2934264614941158
>100	0.0023163434235138163	0.20021069751018902
>500	2.9817657422996223E-4	0.1429760782212093
>1k	4.4726486134494335E-4	0.4542870290530058
>5k	2.2363243067247167E-4	0.9841178656246103
>10k+	4.4726486134494335E-4	9.238835596622481
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	74801	2.1857806931520525	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	60392	1.7647313220523622	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	48550	1.4186929673738606	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	17031	0.4976675577207872	No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG	13556	0.3961236223629259	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	11187	0.3268984186614084	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	8481	0.24782564482590547	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	7808	0.22815972583429667	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	7733	0.22596813010714858	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.3149574362888511E-4	0.0
12-13	0.0	0.0	0.0	2.48380849076783E-4	0.0
14-15	0.0	0.0	0.0	4.090978690676426E-4	0.0
16-17	0.0	0.0	0.0	7.159212708683746E-4	0.0
18-19	0.0	0.0	0.0	0.001125019139936017	0.0
20-21	0.0	0.0	0.0	0.0016510021144515575	0.0
22-23	0.0	0.0	0.0	0.002513029767129804	0.0
24-25	0.0	0.0	0.0	0.003944872308866553	0.0
26-27	0.0	0.0	0.0	0.005537431870594162	0.0
28-29	0.0	0.0	0.0	0.009906012686709344	0.0
30-31	0.0	0.0	0.0	0.019388316866170062	0.0
32-33	0.0	0.0	0.0	0.04320365710117925	0.0
34-35	0.0	0.0	0.0	0.09358113754922325	0.0
36-37	0.0	0.0	0.0	0.1574734583146804	0.0
38-39	0.0	0.0	0.0	0.22779445987977198	0.0
40-41	0.0	0.0	0.0	0.3056983826607959	0.0
42-43	0.0	0.0	0.0	0.37582944592953466	0.0
44-45	0.0	0.0	0.0	0.4558373006086207	0.0
46-47	0.0	0.0	0.0	0.5603325848790415	0.0
48-49	0.0	0.0	0.0	0.6705990712309521	0.0
50-51	0.0	0.0	0.0	0.7746560363559432	0.0
52-53	1.4610638180987235E-5	0.0	0.0	0.8725180908921957	0.0
54-55	2.922127636197447E-5	0.0	0.0	0.9773640304789601	0.0
56-57	2.922127636197447E-5	0.0	0.0	1.1086260038969495	0.0
58-59	2.922127636197447E-5	0.0	0.0	1.2790444876399845	0.0
60-61	2.922127636197447E-5	0.0	0.0	1.4759082264906065	0.0
62-63	2.922127636197447E-5	0.0	0.0	1.6373557783905155	0.0
64-65	2.922127636197447E-5	0.0	0.0	1.777793232586165	0.0
66-67	2.922127636197447E-5	0.0	0.0	1.9346530440972436	0.0
68-69	2.922127636197447E-5	0.0	0.0	2.108548859727354	0.0
70-71	2.922127636197447E-5	0.0	0.0	2.3064937858033687	0.0
72-73	2.922127636197447E-5	0.0	0.0	2.5037227906085153	0.0
74-75	2.922127636197447E-5	0.0	0.0	2.6903736933706277	0.0
76-77	2.922127636197447E-5	0.0	0.0	2.867498460038736	0.0
78-79	2.922127636197447E-5	0.0	0.0	3.083750515755528	0.0
80-81	2.922127636197447E-5	2.922127636197447E-5	0.0	3.3191863394039562	0.0
82-83	2.922127636197447E-5	2.922127636197447E-5	0.0	3.555031260921452	0.0
84-85	2.922127636197447E-5	2.922127636197447E-5	0.0	3.852883730879058	0.0
86-87	2.922127636197447E-5	2.922127636197447E-5	0.0	4.153541443367413	0.0
88	2.922127636197447E-5	2.922127636197447E-5	0.0	4.348798012018126	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1360	0.0	26.274986	1
TACCCCG	270	4.7293724E-11	22.626907	5
TAGGCAT	1805	0.0	22.390657	5
GCATCGT	1485	0.0	21.518038	8
TAGTACT	1930	0.0	20.940485	4
TATTCGC	270	1.1477823E-9	20.885155	9
GGGCACC	1780	0.0	20.861614	94
GCTATTG	1845	0.0	20.375761	8
CTATTGA	1780	0.0	20.327826	9
CTAGTAC	2035	0.0	20.090652	3
CTAGGCA	2120	0.0	19.728756	4
GGGCTAT	1940	0.0	19.378262	6
TACCTGG	3390	0.0	19.268785	2
TTTAGGG	1960	0.0	19.181084	2
CCGTATC	295	3.441528E-9	19.120535	94
GTACCCG	615	0.0	19.113167	1
ATACGGG	840	0.0	19.021242	3
CTAACGC	550	0.0	18.79746	3
CATGGGG	5900	0.0	18.718086	4
TACGGGC	855	0.0	18.687809	4
>>END_MODULE