FastQCFastQC Report
Mon 16 Jan 2017
ERR1146566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3422164
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA747052.182975450621303No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA606941.7735561475136785No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT540791.5802574043792175No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA168520.49243694925199377No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG142270.41573109880181075No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG137190.4008866904099278No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA91740.2680759893447538No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA89870.26261161066506455No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73490.2147471599841504No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC70850.20703274302458913No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA69170.20212356859577743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68120.19905533457777008No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC42520.12424886709111545No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG36300.10607323319396732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35870.10481671831040242No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG35140.1026835651359783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG17300.024.4491529
TATTCGC1802.1649612E-723.4983549
CTATTGA18350.023.0501569
CTAACGC5400.022.6343253
GCTATTG18750.022.558428
CTAGTAC21000.022.3855973
GGGCTAT19000.022.2638746
GGCTATT18900.022.1307227
TAGTACT19800.022.080344
GAGTACT86800.022.009412-13
TACGGGC9650.021.9216484
TAACGCC6300.021.6394144
AGGGCTA19600.021.5861135
ATACGGG9850.021.4765383
GTACCCG5100.021.2218781
GGGCACC19150.020.61474294
CCTAGTA22700.020.5209472
ATTTAGG21050.020.3430211
CGATAAT9800.020.14379966-67
CGGGCGT10350.019.981326
Produced by FastQC (version 0.11.5)