##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3422164
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.498874980860066	34.0	31.0	34.0	31.0	34.0
2	32.498680074946726	34.0	31.0	34.0	31.0	34.0
3	32.68424423844094	34.0	31.0	34.0	31.0	34.0
4	36.072645261886926	37.0	35.0	37.0	35.0	37.0
5	36.130362542531564	37.0	35.0	37.0	35.0	37.0
6	36.26080602799866	37.0	37.0	37.0	35.0	37.0
7	36.279991549206876	37.0	37.0	37.0	35.0	37.0
8	36.297814774511096	37.0	37.0	37.0	35.0	37.0
9	38.119553007979746	39.0	39.0	39.0	37.0	39.0
10-11	38.09340800148678	39.0	38.0	39.0	37.0	39.0
12-13	38.08471642504567	39.0	38.0	39.0	37.0	39.0
14-15	39.43478483205364	41.0	39.0	41.0	36.5	41.0
16-17	39.45447982621523	41.0	39.0	41.0	36.5	41.0
18-19	39.573861743621876	41.0	39.5	41.0	37.0	41.0
20-21	39.60404746821017	41.0	40.0	41.0	37.0	41.0
22-23	39.57599226688143	41.0	39.5	41.0	37.0	41.0
24-25	39.55027564429992	41.0	39.0	41.0	37.0	41.0
26-27	39.3716144229207	41.0	39.0	41.0	36.0	41.0
28-29	39.25480558500411	41.0	39.0	41.0	36.0	41.0
30-31	39.24647883619838	41.0	39.0	41.0	36.0	41.0
32-33	39.16452250681148	41.0	39.0	41.0	35.0	41.0
34-35	39.1029613133678	41.0	39.0	41.0	35.0	41.0
36-37	38.981539604764706	40.0	39.0	41.0	35.0	41.0
38-39	38.81912994234058	40.0	38.0	41.0	35.0	41.0
40-41	38.74933346268618	40.0	38.0	41.0	35.0	41.0
42-43	38.75210510074912	40.0	38.0	41.0	35.0	41.0
44-45	38.670805081229304	40.0	38.0	41.0	35.0	41.0
46-47	38.48455202614486	40.0	38.0	41.0	35.0	41.0
48-49	38.28778194148498	40.0	38.0	41.0	34.0	41.0
50-51	38.103434698044865	40.0	38.0	41.0	34.0	41.0
52-53	37.873911945774665	40.0	37.0	41.0	33.0	41.0
54-55	37.76032226976849	40.0	37.0	41.0	33.0	41.0
56-57	37.61946256812941	40.0	36.5	41.0	33.0	41.0
58-59	37.43354102842529	39.5	36.0	41.0	33.0	41.0
60-61	37.133663670122175	39.0	35.5	41.0	32.0	41.0
62-63	36.81010071405111	39.0	35.0	41.0	32.0	41.0
64-65	36.41610703052221	38.0	35.0	40.0	31.0	41.0
66-67	36.123309987481605	37.0	35.0	39.5	31.0	41.0
68-69	35.793164500590855	37.0	35.0	39.0	31.0	41.0
70-71	35.39830338347315	36.0	35.0	39.0	31.0	41.0
72-73	34.96779318583212	35.5	35.0	37.5	30.5	40.0
74-75	34.63872333412425	35.0	34.0	37.0	30.0	39.5
76-77	34.22722537552262	35.0	34.0	36.5	29.5	39.0
78-79	33.92606023557024	35.0	34.0	36.0	29.0	39.0
80-81	33.74918910958095	35.0	34.0	36.0	29.0	37.5
82-83	33.53355902873153	35.0	34.0	35.5	29.0	37.0
84-85	33.37342468099132	35.0	34.0	35.0	29.0	37.0
86-87	33.19939868457502	35.0	34.0	35.0	29.0	36.0
88-89	32.956771797026676	35.0	34.0	35.0	28.5	36.0
90-91	32.82042795143658	35.0	33.0	35.0	28.0	36.0
92-93	32.835395235295564	35.0	33.5	35.0	29.0	36.0
94-95	32.82338178415763	35.0	34.0	35.0	29.0	35.0
96-97	32.753402963738736	35.0	34.0	35.0	29.0	35.0
98-99	32.69552671935068	35.0	34.0	35.0	29.0	35.0
100	31.920113121405052	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20189970728500128
1101	2	0.22702580780551784
1101	3	0.22863949652966653
1101	4	0.1813962555634916
1101	5	0.1472092661950839
1101	6	0.16423414421601024
1101	7	0.1501519492112422
1101	8	0.16940185337176672
1101	9	0.1981030987343786
1101	10-11	0.17815990404659487
1101	12-13	0.1618574390077967
1101	14-15	0.2730002739598376
1101	16-17	0.34229132578280996
1101	18-19	0.2856524518912593
1101	20-21	0.29644676887541976
1101	22-23	0.31242168291365147
1101	24-25	0.29308707024846825
1101	26-27	0.32442791005717453
1101	28-29	0.3281228920050765
1101	30-31	0.361542960694095
1101	32-33	0.35889200817001665
1101	34-35	0.35644206976328974
1101	36-37	0.3794463102474026
1101	38-39	0.391624004361546
1101	40-41	0.3853906154549165
1101	42-43	0.33547536661513533
1101	44-45	0.43854406978016414
1101	46-47	0.44153000025205813
1101	48-49	0.45669378003339034
1101	50-51	0.4286712922739184
1101	52-53	0.4913365790842903
1101	54-55	0.459747030247847
1101	56-57	0.4527092528056542
1101	58-59	0.4318913455491611
1101	60-61	0.3743831130137494
1101	62-63	0.33212750960965565
1101	64-65	0.43845073295781845
1101	66-67	0.4286795374033332
1101	68-69	0.4952949184151336
1101	70-71	0.368734303237801
1101	72-73	0.3524288256267454
1101	74-75	0.407712600930104
1101	76-77	0.3948382539752373
1101	78-79	0.3668944385038202
1101	80-81	0.39906481565312646
1101	82-83	0.35430280365589795
1101	84-85	0.37169733593033527
1101	86-87	0.3941174069636304
1101	88-89	0.3839862128966871
1101	90-91	0.38379760947925945
1101	92-93	0.31746179852687106
1101	94-95	0.3454036521609751
1101	96-97	0.348915005208255
1101	98-99	0.40276754591217667
1101	100	0.3927776338110114
1102	1	0.23051322257250462
1102	2	0.2430092034992768
1102	3	0.2522592388809741
1102	4	0.200919694375294
1102	5	0.10472021855422753
1102	6	0.12255905956475743
1102	7	0.1521924686749685
1102	8	0.16038091660946208
1102	9	0.1745170283214108
1102	10-11	0.18136479913830783
1102	12-13	0.21989708569488187
1102	14-15	0.31200517390030313
1102	16-17	0.3072951823421448
1102	18-19	0.30175639312430036
1102	20-21	0.2844317233554534
1102	22-23	0.3206699609307293
1102	24-25	0.292513300419067
1102	26-27	0.2972120046905786
1102	28-29	0.32857050430548185
1102	30-31	0.3370172187217122
1102	32-33	0.31424862991382696
1102	34-35	0.3187292743369241
1102	36-37	0.38757606304149306
1102	38-39	0.4169783004968153
1102	40-41	0.3794877001833896
1102	42-43	0.3354648160744489
1102	44-45	0.306751878616943
1102	46-47	0.3043830729735646
1102	48-49	0.29950015202120994
1102	50-51	0.3003794113414813
1102	52-53	0.30244890238517996
1102	54-55	0.3732000471188357
1102	56-57	0.37445701496863393
1102	58-59	0.38911024996305343
1102	60-61	0.3439760058103758
1102	62-63	0.29125763324403664
1102	64-65	0.279027573668003
1102	66-67	0.15637995951474437
1102	68-69	0.21038879128964538
1102	70-71	0.19096263130484203
1102	72-73	0.24673306011991514
1102	74-75	0.23437215608340267
1102	76-77	0.2408210121469665
1102	78-79	0.23516555058468214
1102	80-81	0.22083071576689406
1102	82-83	0.22517271593825683
1102	84-85	0.25930669116210225
1102	86-87	0.2952952064710388
1102	88-89	0.33954082563796817
1102	90-91	0.23834359053138598
1102	92-93	0.30357594011243094
1102	94-95	0.3173169904687754
1102	96-97	0.22094615040434462
1102	98-99	0.24130454059739748
1102	100	0.3166278718042683
1103	1	0.18660116186744347
1103	2	0.19953394034344285
1103	3	0.23944814304798712
1103	4	0.1572133986143882
1103	5	0.11877285755574718
1103	6	0.14163251990236603
1103	7	0.1123943357396513
1103	8	0.129863458418626
1103	9	0.17747971053258738
1103	10-11	0.21431284961094832
1103	12-13	0.23656933475175634
1103	14-15	0.3137658793577316
1103	16-17	0.3201132316787252
1103	18-19	0.2855415083174151
1103	20-21	0.3094709959602113
1103	22-23	0.3016794529528042
1103	24-25	0.31901119282192525
1103	26-27	0.334407421646155
1103	28-29	0.38394708059618665
1103	30-31	0.39128319317199356
1103	32-33	0.3003073938746965
1103	34-35	0.3336856729313382
1103	36-37	0.37191765439486346
1103	38-39	0.41398746149295107
1103	40-41	0.45353614749542004
1103	42-43	0.3960321338241002
1103	44-45	0.2980087782553582
1103	46-47	0.39771711145092326
1103	48-49	0.3539810974626505
1103	50-51	0.39564882532761203
1103	52-53	0.3921785286196666
1103	54-55	0.44016165006789976
1103	56-57	0.45597151743076125
1103	58-59	0.4492404273851207
1103	60-61	0.41310543695502133
1103	62-63	0.4160850688310873
1103	64-65	0.40237587172454425
1103	66-67	0.3518120952784827
1103	68-69	0.3662345331990977
1103	70-71	0.347101203858287
1103	72-73	0.37781864893477035
1103	74-75	0.33994622166962785
1103	76-77	0.3164854145178211
1103	78-79	0.2949261150132969
1103	80-81	0.29505187439687575
1103	82-83	0.3026994820302278
1103	84-85	0.3193010793908968
1103	86-87	0.35711797439638104
1103	88-89	0.36262503746323915
1103	90-91	0.31296868895773855
1103	92-93	0.28137222792088323
1103	94-95	0.3328344682469506
1103	96-97	0.34372940284198705
1103	98-99	0.3746124103964661
1103	100	0.49703387806438215
1104	1	0.14718361963593907
1104	2	0.18720827073428836
1104	3	0.15249102086664124
1104	4	0.13463717567771738
1104	5	0.057816696562298375
1104	6	0.0602481699563171
1104	7	0.11581944226210084
1104	8	0.07710934206701836
1104	9	0.10509351554770774
1104	10-11	0.13438749818813278
1104	12-13	0.1530293091937267
1104	14-15	0.21774965332758
1104	16-17	0.17745996640191208
1104	18-19	0.14508884774195252
1104	20-21	0.17198098531717676
1104	22-23	0.17563420715017486
1104	24-25	0.2004301142291567
1104	26-27	0.2123707620871258
1104	28-29	0.27332830207818404
1104	30-31	0.2004457352009439
1104	32-33	0.16254831506834222
1104	34-35	0.149320351559453
1104	36-37	0.25758334146007655
1104	38-39	0.24725768234520729
1104	40-41	0.24021458042770405
1104	42-43	0.2403869502593139
1104	44-45	0.21775704645992988
1104	46-47	0.16159467599966604
1104	48-49	0.16291208895927412
1104	50-51	0.2352433679163397
1104	52-53	0.24652279408429933
1104	54-55	0.23732158841929873
1104	56-57	0.1984602115041767
1104	58-59	0.14941609275915368
1104	60-61	0.13142172853744682
1104	62-63	0.23927704125492966
1104	64-65	0.24946873867920516
1104	66-67	0.2731857285318924
1104	68-69	0.19361319703744329
1104	70-71	0.20905961498891656
1104	72-73	0.25349295354663326
1104	74-75	0.26642538304239594
1104	76-77	0.2908514356050276
1104	78-79	0.2553395362897959
1104	80-81	0.22979195777347883
1104	82-83	0.19201031767446608
1104	84-85	0.24508434625126796
1104	86-87	0.24976944435097437
1104	88-89	0.2371795673834356
1104	90-91	0.19995076151453617
1104	92-93	0.20756008675515858
1104	94-95	0.17749508790010537
1104	96-97	0.14773835735680763
1104	98-99	0.07680730689384063
1104	100	0.10120160351081964
1105	1	0.12654346172465836
1105	2	0.11811397359810627
1105	3	0.12746967415724697
1105	4	0.08105792756172292
1105	5	0.07168933916841524
1105	6	0.06391745656801362
1105	7	0.09912128412885579
1105	8	0.11854879810435648
1105	9	0.1124019695005174
1105	10-11	0.1209393733241626
1105	12-13	0.08862416823985342
1105	14-15	0.13068519826113345
1105	16-17	0.16327042419238325
1105	18-19	0.09348711528841847
1105	20-21	0.1039012070502423
1105	22-23	0.09789132112339871
1105	24-25	0.1323332913893296
1105	26-27	0.13301442384478435
1105	28-29	0.16721068986491616
1105	30-31	0.16424556511768174
1105	32-33	0.16427210392414082
1105	34-35	0.1305592391451924
1105	36-37	0.17989189120770277
1105	38-39	0.15581570630011754
1105	40-41	0.14768455652042434
1105	42-43	0.10727526541493404
1105	44-45	0.12542469644741772
1105	46-47	0.1318857265373765
1105	48-49	0.14087069455268875
1105	50-51	0.18349807269312635
1105	52-53	0.13723579747957615
1105	54-55	0.1266840143789736
1105	56-57	0.14047189916198732
1105	58-59	0.11999724624264729
1105	60-61	0.0989914296213783
1105	62-63	0.11642174475962719
1105	64-65	0.12208329676391827
1105	66-67	0.06060944004417479
1105	68-69	0.07045899482960039
1105	70-71	0.0182013055309298
1105	72-73	0.022664818592282643
1105	74-75	0.057289207174854084
1105	76-77	0.01165697333929927
1105	78-79	0.02599885316402606
1105	80-81	0.033233209575250555
1105	82-83	0.028778973887241932
1105	84-85	-0.03724152768654676
1105	86-87	0.028437984453532295
1105	88-89	0.040137443728035294
1105	90-91	0.0140440515106377
1105	92-93	-0.019596690348258505
1105	94-95	-0.004722011457062081
1105	96-97	-0.03921441476389731
1105	98-99	-0.02874131864042795
1105	100	0.04830405200902632
1106	1	0.14455830860871544
1106	2	0.19099573989964824
1106	3	0.1306990091469018
1106	4	0.11160901505754595
1106	5	0.09916503591511372
1106	6	0.0853683224581232
1106	7	0.039631063596026195
1106	8	0.03819993455815052
1106	9	0.09773085729140263
1106	10-11	0.09405797533781168
1106	12-13	0.09521563311076875
1106	14-15	0.14992101411833403
1106	16-17	0.15244941838771808
1106	18-19	0.15170642232146747
1106	20-21	0.1351303274634077
1106	22-23	0.1694162816713316
1106	24-25	0.12606529440326852
1106	26-27	0.18942915086197587
1106	28-29	0.23022756947064948
1106	30-31	0.21493810866705587
1106	32-33	0.174151316272237
1106	34-35	0.189615271149151
1106	36-37	0.2222682600063095
1106	38-39	0.24479810815090275
1106	40-41	0.24067337264605726
1106	42-43	0.2110618004527609
1106	44-45	0.16840551733812248
1106	46-47	0.1985270783856805
1106	48-49	0.25720989229149893
1106	50-51	0.30504292883849615
1106	52-53	0.28694433108772444
1106	54-55	0.25845442658368967
1106	56-57	0.23841196490788974
1106	58-59	0.284748290247677
1106	60-61	0.2149938614882032
1106	62-63	0.21905195096270091
1106	64-65	0.2411266720437979
1106	66-67	0.18969657741710222
1106	68-69	0.15680019446013915
1106	70-71	0.11111380724199194
1106	72-73	0.08321630893284038
1106	74-75	0.12512014310240005
1106	76-77	0.170913482262101
1106	78-79	0.12788655073779864
1106	80-81	0.15286923935122587
1106	82-83	0.19017349906277303
1106	84-85	0.15053885533658473
1106	86-87	0.1944831260842932
1106	88-89	0.21243635945907613
1106	90-91	0.2626715841400369
1106	92-93	0.11763633765890091
1106	94-95	0.1637741526799701
1106	96-97	0.271849880490052
1106	98-99	0.21396838362852577
1106	100	0.25997010687236966
1107	1	0.07362726255115604
1107	2	0.09056171920761358
1107	3	0.13887731281086957
1107	4	0.08760810879049075
1107	5	0.08124161388892048
1107	6	0.06465816904176336
1107	7	0.037861612998526084
1107	8	0.027205035694926494
1107	9	0.04876460299748686
1107	10-11	0.05878409832653375
1107	12-13	0.06149413662334524
1107	14-15	0.11211152053287776
1107	16-17	0.13817989313070456
1107	18-19	0.12929873035988493
1107	20-21	0.09124727175068159
1107	22-23	0.06855423295827734
1107	24-25	0.08624751938446451
1107	26-27	0.08273941871645718
1107	28-29	0.12025164368261443
1107	30-31	0.0945316900982931
1107	32-33	0.10848145709801571
1107	34-35	0.07187752237864231
1107	36-37	0.07158939589818658
1107	38-39	0.03433944384360643
1107	40-41	0.06405295241049203
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2216	34-35	-0.5506506894097569
2216	36-37	-0.5969540138285225
2216	38-39	-0.6134120641032226
2216	40-41	-0.5802789774907353
2216	42-43	-0.6797584292863803
2216	44-45	-0.6698582778551625
2216	46-47	-0.685555800037676
2216	48-49	-0.6630831264728414
2216	50-51	-0.698735439135362
2216	52-53	-0.7373823312174821
2216	54-55	-0.7361831317538261
2216	56-57	-0.7937930616479747
2216	58-59	-0.7883942025112844
2216	60-61	-0.8011184325760112
2216	62-63	-0.8867522512885984
2216	64-65	-0.8010907326733161
2216	66-67	-0.8487165299002797
2216	68-69	-0.7886548869095051
2216	70-71	-0.7785139643994015
2216	72-73	-0.7561719368668847
2216	74-75	-0.8217130835583148
2216	76-77	-0.7862521699804148
2216	78-79	-0.8242504328677143
2216	80-81	-0.7687037918001351
2216	82-83	-0.726753924548099
2216	84-85	-0.7668225426480362
2216	86-87	-0.818806229169823
2216	88-89	-0.8192996621425692
2216	90-91	-0.8835314614372436
2216	92-93	-0.9531403867882737
2216	94-95	-0.9391373155431104
2216	96-97	-0.943494873019354
2216	98-99	-0.9581631422391368
2216	100	-1.2061080578620746
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	23.0
8	60.0
9	107.0
10	139.0
11	176.0
12	140.0
13	162.0
14	274.0
15	507.0
16	849.0
17	1595.0
18	2975.0
19	4671.0
20	6969.0
21	10053.0
22	14798.0
23	18269.0
24	19089.0
25	18395.0
26	18112.0
27	20115.0
28	24958.0
29	30597.0
30	38291.0
31	48714.0
32	63660.0
33	85228.0
34	127025.0
35	205276.0
36	399791.0
37	923559.0
38	1034968.0
39	301722.0
40	893.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.03637446481711	17.328163588071284	12.49052809193325	22.144933855178362
2	13.5414671238786	22.667466056400844	38.07441636019331	25.71665045952724
3	23.2876836419699	26.148606080157844	30.327998672178996	20.235711605693265
4	11.801567663034266	15.776946984422722	40.778378826964456	31.643106525578553
5	12.510282508072537	40.6116243351814	34.1505844440196	12.727508712726458
6	27.53895171614904	37.22947416900035	18.650242709438622	16.58133140541199
7	25.667384730831134	34.696350028812176	21.534941048997066	18.10132419135962
8	23.214784563217894	30.966838526733376	20.978421840683264	24.839955069365466
9	24.5622068375449	19.594122315587448	22.277687451565733	33.56598339530192
10-11	24.408546755795456	23.67203909573007	28.256565144160245	23.66284900431423
12-13	31.077499500316176	21.59727003147716	25.86809106752336	21.45713940068331
14-15	26.083434085903285	23.51733735613081	28.400448546788766	21.998780011177143
16-17	21.10623570349054	26.564697074716463	30.306335406485484	22.02273181530751
18-19	20.234039046638326	30.848828402145546	28.77261288471271	20.144519666503417
20-21	22.049732274665974	27.60972297061158	25.791239110691365	24.549305644031087
22-23	20.929371886094998	26.91765410633666	25.07531814794793	27.077655859620414
24-25	23.735770699475538	26.822501785419984	26.251649541050632	23.190077974053843
26-27	25.279063189256856	23.243655184263528	29.078866471624387	22.39841515485523
28-29	21.660683365946717	26.446793088641147	29.87160023184164	22.020923313570496
30-31	22.129777373095155	27.963226739365382	27.69604172568762	22.21095416185185
32-33	22.045679121115462	27.60515769435265	26.797746595266286	23.5514165892656
34-35	23.60165365208288	25.301388246621798	27.36456687333665	23.732391227958672
36-37	25.076450767459047	24.319569906172664	29.298388696887358	21.305590629480935
38-39	25.697151082567586	23.601460722553302	29.58341588396975	21.117972310909366
40-41	22.27569626115994	25.716869745121258	30.53384344634572	21.47359054737308
42-43	21.55902621422971	27.888119424373397	27.265645066886336	23.287209294510554
44-45	22.386972620838996	27.510102212164206	25.7725816238434	24.330343543153397
46-47	23.15309413130202	26.730999405053424	25.023640067840276	25.09226639580428
48-49	24.27192679219987	26.164611768803752	26.126127179149712	23.437334259846665
50-51	23.951616190645815	27.045735902118455	27.547264698529705	21.45538320870602
52-53	22.21987313774989	27.70109729680606	27.474279960153552	22.604749605290493
54-55	20.686440285454754	26.311873770589767	28.509752073167544	24.491933870787935
56-57	23.84393705444442	27.146191321667313	25.485866705317033	23.52400491857123
58-59	23.798770136289658	25.889614420909584	26.282021217172623	24.029594225628138
60-61	23.03139672056033	25.02922905144167	28.67814847510702	23.26122575289098
62-63	25.5237636320415	24.697246349898112	26.81694926596166	22.962040752098726
64-65	23.34358507098801	27.036548426127656	27.94200737780286	21.677859125081476
66-67	22.57076467948762	25.64581212576108	27.971128820230458	23.812294374520842
68-69	21.518378668468316	28.236381012968394	27.018836507866702	23.226403810696596
70-71	22.81871340073513	27.563236121994166	24.849826035920188	24.76822444135052
72-73	22.75870634226597	25.56294158172006	25.999425774820445	25.678926301193528
74-75	22.838028196795758	28.120598862291978	25.913489714003763	23.127883226908505
76-77	24.472821426499785	24.665278517057626	26.69320817290981	24.168691883532777
78-79	23.014326131792465	25.206376832261	28.576989005119025	23.202308030827513
80-81	22.9265739026966	27.79755897003123	27.196316246863823	22.07955088040835
82-83	22.48732123678046	26.451309472863553	27.064153497228872	23.997215793127115
84-85	22.75141683156006	26.024685282879044	26.443587318595956	24.780310566964932
86-87	23.93608254893687	26.3005513470424	26.912050386831254	22.851315717189475
88-89	24.731426778073953	24.342797731723667	27.01077799447016	23.914997495732226
90-91	23.00189295428273	24.675030185578482	28.474614308373297	23.848462551765493
92-93	22.718724175697012	27.818596654046974	26.991532258535827	22.471146911720187
94-95	21.82381435367047	28.105207145305467	26.17458607319406	23.89639242783
96-97	20.962227580122807	26.553123136224478	26.136119571509514	26.348529712143204
98-99	22.95871806942476	27.043926466131975	25.120647803092268	24.876707661351006
100	21.77213673315528	27.63060541064948	26.33457427223901	24.26268358395624
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	378.0
1	242.0
2	297.0
3	515.0
4	335.0
5	176.0
6	390.0
7	884.5
8	1601.0
9	1959.5
10	1479.5
11	971.5
12	1006.0
13	1162.0
14	1335.5
15	1572.0
16	1818.5
17	2167.0
18	2634.0
19	3139.0
20	3636.5
21	4365.0
22	5344.0
23	6601.0
24	8207.0
25	10184.0
26	12788.5
27	15774.0
28	18756.5
29	22068.5
30	26158.5
31	30426.0
32	36013.0
33	41282.0
34	45189.0
35	49567.5
36	55574.0
37	62648.0
38	64432.5
39	62979.5
40	63995.5
41	65457.5
42	68156.0
43	72360.0
44	80495.0
45	94567.0
46	113757.5
47	181366.0
48	440143.0
49	446321.0
50	224453.0
51	163137.0
52	112634.5
53	95322.5
54	88626.5
55	78760.0
56	71239.0
57	63926.5
58	56400.0
59	51808.0
60	43531.5
61	33802.5
62	26629.5
63	21184.5
64	15699.0
65	10555.0
66	7235.5
67	4967.5
68	3717.0
69	2986.5
70	2481.5
71	2071.5
72	1761.5
73	1705.5
74	1436.0
75	1087.0
76	811.0
77	563.5
78	397.0
79	267.5
80	187.0
81	114.0
82	68.0
83	44.5
84	29.0
85	13.0
86	8.5
87	6.0
88	3.0
89	3.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005931919101480818
2	0.08442026740974425
3	4.675404217915915E-4
4	0.0
5	0.03816298692873866
6	0.004996838257897635
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.383191454296171E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.090978690676426E-4
24-25	0.0
26-27	0.0
28-29	1.4610638180987235E-5
30-31	1.0227446726691065E-4
32-33	0.0015633382853656342
34-35	0.0012126829690219405
36-37	7.889744617733108E-4
38-39	6.428680799634383E-4
40-41	0.0013587893508318128
42-43	4.3831914542961703E-4
44-45	5.113723363345532E-4
46-47	2.3377021089579576E-4
48-49	4.3831914542961703E-4
50-51	8.03585099954298E-4
52-53	7.889744617733108E-4
54-55	0.0013295680744698384
56-57	9.35080843583183E-4
58-59	0.0022646489180530214
60-61	0.001607170199908596
62-63	9.789127581261447E-4
64-65	0.004164031881581362
66-67	0.0039594829470475405
68-69	0.003287393590722128
70-71	0.0014464531799177362
72-73	0.004719236132458877
74-75	0.00317050848527423
76-77	0.002995180827102383
78-79	7.597531854113362E-4
80-81	5.844255272394894E-5
82-83	1.3149574362888511E-4
84-85	1.0227446726691065E-4
86-87	0.0
88-89	1.7532765817184683E-4
90-91	0.0
92-93	0.0
94-95	2.922127636197447E-5
96-97	3.2143403998171916E-4
98-99	3.798765927056681E-4
100	7.013106326873873E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3422164.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.13940644784264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97073697700753	36.294013641701234
2	15.51721648090722	15.870824811125535
3	5.836484705013489	8.954230906689054
4	2.9480129354694102	6.030385268818715
5	1.593819318871597	4.075348697609916
6	0.9400377468483293	2.8843783447434554
7	0.5781164987739085	2.069517422350184
8	0.391911361556738	1.603369152814195
9	0.2747592066969912	1.2645920471907819
>10	0.926936847687903	7.04111667094897
>50	0.014041456369087905	0.4915179156114854
>100	0.005366626196932261	0.5217463201245103
>500	7.199546064992007E-4	0.24856275485468318
>1k	8.799445190542498E-4	1.171345845910478
>5k	4.7996973766595443E-4	1.895066006564348
>10k+	4.7996973766595443E-4	9.583984192942518
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	74705	2.182975450621303	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	60694	1.7735561475136785	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	54079	1.5802574043792175	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	16852	0.49243694925199377	No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG	14227	0.41573109880181075	No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG	13719	0.4008866904099278	No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA	9174	0.2680759893447538	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	8987	0.26261161066506455	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7349	0.2147471599841504	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	7085	0.20703274302458913	No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA	6917	0.20212356859577743	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6812	0.19905533457777008	No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC	4252	0.12424886709111545	No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG	3630	0.10607323319396732	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3587	0.10481671831040242	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	3514	0.1026835651359783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.3149574362888511E-4	0.0
12-13	0.0	0.0	0.0	2.48380849076783E-4	0.0
14-15	0.0	0.0	0.0	4.090978690676426E-4	0.0
16-17	0.0	0.0	0.0	7.159212708683746E-4	0.0
18-19	0.0	0.0	0.0	0.0010957978635740427	0.0
20-21	0.0	0.0	0.0	0.0016363914762705704	0.0
22-23	0.0	0.0	0.0	0.002513029767129804	0.0
24-25	0.0	0.0	0.0	0.003930261670685566	0.0
26-27	0.0	0.0	0.0	0.005552042508775149	0.0
28-29	0.0	0.0	0.0	0.009964455239433293	0.0
30-31	0.0	0.0	0.0	0.019432148780713022	0.0
32-33	0.0	0.0	0.0	0.043408206035713076	0.0
34-35	0.0	0.0	0.0	0.09409250988555778	0.0
36-37	0.0	0.0	0.0	0.15798483065101498	0.0
38-39	0.0	0.0	0.0	0.22846654923609738	0.0
40-41	0.0	0.0	0.0	0.30669190605710306	0.0
42-43	0.0	0.0	0.0	0.37707135017491855	0.0
44-45	0.0	0.0	0.0	0.4571522580449096	0.0
46-47	0.0	0.0	0.0	0.561968976355312	0.0
48-49	0.0	0.0	0.0	0.6731413222744439	0.0
50-51	0.0	0.0	0.0	0.7775781639921406	0.0
52-53	0.0	0.0	0.0	0.8758785376738227	0.0
54-55	0.0	0.0	0.0	0.980943636833302	0.0
56-57	0.0	0.0	0.0	1.112410159185825	0.0
58-59	0.0	0.0	0.0	1.283003970587032	0.0
60-61	0.0	0.0	0.0	1.4800284264576449	0.0
62-63	0.0	0.0	0.0	1.6415490315484589	0.0
64-65	0.0	0.0	0.0	1.7823809729749946	0.0
66-67	0.0	0.0	0.0	1.939985927033304	0.0
68-69	0.0	0.0	0.0	2.1147437703160925	0.0
70-71	0.0	0.0	0.0	2.3134192283011568	0.0
72-73	0.0	0.0	0.0	2.511641756502611	0.0
74-75	0.0	0.0	0.0	2.6990378018119525	0.0
76-77	0.0	0.0	0.0	2.8773314195345403	0.0
78-79	0.0	0.0	0.0	3.0947231050294493	0.0
80-81	0.0	0.0	0.0	3.331766098877786	0.0
82-83	0.0	0.0	0.0	3.568589933153408	0.0
84-85	0.0	0.0	0.0	3.867976520120018	0.0
86-87	0.0	0.0	0.0	4.170840438973702	0.0
88	0.0	0.0	0.0	4.367674956547962	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTGCG	1730	0.0	24.449152	9
TATTCGC	180	2.1649612E-7	23.498354	9
CTATTGA	1835	0.0	23.050156	9
CTAACGC	540	0.0	22.634325	3
GCTATTG	1875	0.0	22.55842	8
CTAGTAC	2100	0.0	22.385597	3
GGGCTAT	1900	0.0	22.263874	6
GGCTATT	1890	0.0	22.130722	7
TAGTACT	1980	0.0	22.08034	4
GAGTACT	8680	0.0	22.0094	12-13
TACGGGC	965	0.0	21.921648	4
TAACGCC	630	0.0	21.639414	4
AGGGCTA	1960	0.0	21.586113	5
ATACGGG	985	0.0	21.476538	3
GTACCCG	510	0.0	21.221878	1
GGGCACC	1915	0.0	20.614742	94
CCTAGTA	2270	0.0	20.520947	2
ATTTAGG	2105	0.0	20.343021	1
CGATAAT	980	0.0	20.143799	66-67
CGGGCGT	1035	0.0	19.98132	6
>>END_MODULE