FastQCFastQC Report
Mon 16 Jan 2017
ERR1146565_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146565_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4609120
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT209360.4542298746832367No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA132680.2878640608185511No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG60790.13189068629152637No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT55630.12069549067934876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17150.026.318521
TACGCGT1805.680842E-620.8846268
GTATAAG20000.019.9821971
GTGATCG12250.019.9469498
CATGGGG108200.019.6314954
GGGCTAT29850.019.5205156
GTATATA14700.019.510431
TAGGCAT25300.019.5030065
TGATCGC12750.018.7961649
GGCTATT30550.018.6105777
ACGCGTA2051.7459482E-518.337729
CTATTGA30750.018.1849069
ATGGGGG66050.017.5022985
GCTATTG31950.017.354838
TACCTGG43700.017.3137592
CTAGGCA28850.017.2664174
CTAACGC9800.017.263283
ATTTAGG34600.017.1217751
AGGGCTA36550.016.8428925
TTTAGGG35100.016.735932
Produced by FastQC (version 0.11.5)