##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146565_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4609120
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47968202173083	31.0	31.0	34.0	30.0	34.0
2	31.777391562814593	33.0	31.0	34.0	30.0	34.0
3	31.90219759954178	34.0	31.0	34.0	30.0	34.0
4	35.4325127139237	37.0	35.0	37.0	33.0	37.0
5	35.38187398028257	37.0	35.0	37.0	33.0	37.0
6	35.52003896622349	37.0	35.0	37.0	33.0	37.0
7	35.488113783108275	37.0	35.0	37.0	33.0	37.0
8	35.446068880827575	37.0	35.0	37.0	33.0	37.0
9	37.12859309369251	39.0	38.0	39.0	34.0	39.0
10-11	37.10100865241087	39.0	38.0	39.0	34.0	39.0
12-13	37.0701126245357	39.0	38.0	39.0	34.0	39.0
14-15	38.49822688495851	41.0	39.0	41.0	34.0	41.0
16-17	38.50452505033499	41.0	39.0	41.0	34.0	41.0
18-19	38.52959784080258	40.5	39.0	41.0	34.0	41.0
20-21	38.59832755493456	41.0	39.0	41.0	34.0	41.0
22-23	38.60076760856736	41.0	39.0	41.0	34.0	41.0
24-25	38.63113327489846	41.0	39.0	41.0	34.0	41.0
26-27	38.63290780018745	41.0	39.0	41.0	34.0	41.0
28-29	38.58066387943903	40.0	39.0	41.0	34.0	41.0
30-31	38.565643224737045	40.0	39.0	41.0	34.0	41.0
32-33	38.55505866629639	40.0	38.0	41.0	34.0	41.0
34-35	38.482652219946544	40.0	38.0	41.0	33.5	41.0
36-37	38.39552658641997	40.0	38.0	41.0	33.0	41.0
38-39	38.34646613670289	40.0	38.0	41.0	33.0	41.0
40-41	38.27801228867983	40.0	38.0	41.0	33.0	41.0
42-43	38.15190763095775	40.0	38.0	41.0	33.0	41.0
44-45	38.00536534088937	40.0	38.0	41.0	33.0	41.0
46-47	37.875555420557504	40.0	37.0	41.0	33.0	41.0
48-49	37.7424184009095	40.0	37.0	41.0	32.0	41.0
50-51	37.59509168778422	40.0	37.0	41.0	32.0	41.0
52-53	37.46512620630402	40.0	36.0	41.0	32.0	41.0
54-55	37.26221241798868	39.5	36.0	41.0	31.0	41.0
56-57	37.06644543860867	39.0	35.0	41.0	31.0	41.0
58-59	36.823942965251504	39.0	35.0	41.0	31.0	41.0
60-61	36.605652705939534	38.5	35.0	41.0	31.0	41.0
62-63	36.33936402176555	38.0	35.0	40.0	30.5	41.0
64-65	36.07546201878016	37.0	35.0	40.0	30.0	41.0
66-67	35.76434709879543	37.0	35.0	40.0	30.0	41.0
68-69	35.44525831395147	36.0	35.0	39.0	30.0	41.0
70-71	35.14258415923213	36.0	34.0	39.0	29.5	41.0
72-73	34.82835129048495	35.0	34.0	38.5	29.0	40.5
74-75	34.52763521019197	35.0	34.0	37.0	29.0	40.0
76-77	34.2230974676294	35.0	34.0	37.0	29.0	39.0
78-79	33.94887082566737	35.0	34.0	36.5	29.0	39.0
80-81	33.66785818117124	35.0	34.0	36.0	29.0	38.5
82-83	33.44385609400493	35.0	34.0	36.0	28.0	37.0
84-85	33.24864279949318	35.0	34.0	35.0	28.0	37.0
86-87	33.08708755684383	35.0	34.0	35.0	28.0	36.5
88-89	32.9486868426077	35.0	33.0	35.0	27.5	36.0
90-91	32.77762121619745	35.0	33.0	35.0	27.0	36.0
92-93	32.662353442739615	35.0	33.0	35.0	27.0	36.0
94-95	32.57591275558024	35.0	33.0	35.0	27.0	35.5
96-97	32.52461326674072	35.0	33.0	35.0	27.0	35.0
98-99	32.46713504964072	35.0	33.0	35.0	26.5	35.0
100	31.480517973062103	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18374743238665303
1101	2	0.23356790398853278
1101	3	0.2793892659323234
1101	4	0.2737090227591352
1101	5	0.18828762469350124
1101	6	0.23609514635891315
1101	7	0.24859494550162253
1101	8	0.24405756674507018
1101	9	0.4093174554024088
1101	10-11	0.3671507219261514
1101	12-13	0.33050392279783836
1101	14-15	0.41201775704029586
1101	16-17	0.42009012405728186
1101	18-19	0.3116532577645188
1101	20-21	0.35128642351789807
1101	22-23	0.4024111418487024
1101	24-25	0.43362969291325726
1101	26-27	0.3882464511281398
1101	28-29	0.4584367209147402
1101	30-31	0.44227177687558594
1101	32-33	0.43464478626898284
1101	34-35	0.43510769339324185
1101	36-37	0.436364753017898
1101	38-39	0.42664348263004115
1101	40-41	0.4410175320898375
1101	42-43	0.4528872241104054
1101	44-45	0.3378276205678432
1101	46-47	0.3225969536026625
1101	48-49	0.3629425914591877
1101	50-51	0.34873555471673967
1101	52-53	0.3411678846707815
1101	54-55	0.3793591333366848
1101	56-57	0.3515953565164551
1101	58-59	0.34289314030480966
1101	60-61	0.400783203193285
1101	62-63	0.44388255692727085
1101	64-65	0.38765377894915787
1101	66-67	0.38235204965811675
1101	68-69	0.3294247138477786
1101	70-71	0.3212991457804364
1101	72-73	0.33098280495572396
1101	74-75	0.3199672416996293
1101	76-77	0.30265309126966855
1101	78-79	0.2474528415139261
1101	80-81	0.2580772775525233
1101	82-83	0.2643301537368501
1101	84-85	0.3258834538726205
1101	86-87	0.2565933809158949
1101	88-89	0.26135828091626934
1101	90-91	0.2909338403017614
1101	92-93	0.27597659875386427
1101	94-95	0.2482480731772938
1101	96-97	0.2501069861013008
1101	98-99	0.23104736457548114
1101	100	0.13732092077599134
1102	1	0.15442051243798005
1102	2	0.2105740044778308
1102	3	0.3066677828745057
1102	4	0.2741986851688978
1102	5	0.23891896900089193
1102	6	0.32228803054071165
1102	7	0.2905704693445088
1102	8	0.2926787210059061
1102	9	0.44393120542424214
1102	10-11	0.3961917581360552
1102	12-13	0.42800217824350995
1102	14-15	0.4948864561684019
1102	16-17	0.45862598208292127
1102	18-19	0.453440390858745
1102	20-21	0.4459280104967078
1102	22-23	0.4308942374511844
1102	24-25	0.4562122252013552
1102	26-27	0.4470969778962228
1102	28-29	0.4666161636094017
1102	30-31	0.4544547619886856
1102	32-33	0.3902035562032182
1102	34-35	0.4604833390609855
1102	36-37	0.4832852165231287
1102	38-39	0.4380921453590929
1102	40-41	0.43808917508273737
1102	42-43	0.42958015812030936
1102	44-45	0.4158804593598333
1102	46-47	0.3808183363764357
1102	48-49	0.40033841048763463
1102	50-51	0.3690127382292445
1102	52-53	0.3574765566896545
1102	54-55	0.3388013987767451
1102	56-57	0.3396574309156861
1102	58-59	0.37034818171579076
1102	60-61	0.38114449574222675
1102	62-63	0.3648261499180947
1102	64-65	0.33033984695487106
1102	66-67	0.3481105834767817
1102	68-69	0.3537020151669239
1102	70-71	0.32686035860442075
1102	72-73	0.31774561017852676
1102	74-75	0.3048283599929107
1102	76-77	0.2655134617418824
1102	78-79	0.2632168290268808
1102	80-81	0.28286070795648044
1102	82-83	0.31993072391188093
1102	84-85	0.33124288571582383
1102	86-87	0.3198787942271508
1102	88-89	0.28538161873242274
1102	90-91	0.2802586974925987
1102	92-93	0.2953420792605783
1102	94-95	0.32345042207285957
1102	96-97	0.32418783171100785
1102	98-99	0.28031708323334925
1102	100	0.13198092360472202
1103	1	0.16548194648996173
1103	2	0.21554238891104305
1103	3	0.2573383713750417
1103	4	0.19655682132164287
1103	5	0.16896577043745253
1103	6	0.22368863862978827
1103	7	0.2366696076509882
1103	8	0.2854710931293596
1103	9	0.3675600763816007
1103	10-11	0.3913551444202099
1103	12-13	0.3687858600152225
1103	14-15	0.4605580783064127
1103	16-17	0.3800948465039511
1103	18-19	0.3842130012730536
1103	20-21	0.39156708947950136
1103	22-23	0.392293473172856
1103	24-25	0.4120847650371502
1103	26-27	0.4035944846939259
1103	28-29	0.421638825255755
1103	30-31	0.39230223450037727
1103	32-33	0.3882343834357158
1103	34-35	0.4199081813484611
1103	36-37	0.42102586301722766
1103	38-39	0.41136099614368504
1103	40-41	0.39968122106582626
1103	42-43	0.3719957276041157
1103	44-45	0.2663651185194098
1103	46-47	0.31630261891193356
1103	48-49	0.2983128436152427
1103	50-51	0.3167682863871164
1103	52-53	0.3410678922804067
1103	54-55	0.34699773640955556
1103	56-57	0.31567442428113424
1103	58-59	0.3175809271329868
1103	60-61	0.224589856040339
1103	62-63	0.2112684455076561
1103	64-65	0.24211374252556084
1103	66-67	0.2275925155945444
1103	68-69	0.24678681005293157
1103	70-71	0.23767063905945207
1103	72-73	0.2704217661724826
1103	74-75	0.24721403672994313
1103	76-77	0.2656086605305674
1103	78-79	0.22823799426386415
1103	80-81	0.2566345321553385
1103	82-83	0.2121108985787572
1103	84-85	0.24742077484843605
1103	86-87	0.2045540356366402
1103	88-89	0.1324776017660909
1103	90-91	0.24267896203237171
1103	92-93	0.2232530691564989
1103	94-95	0.22330777110446576
1103	96-97	0.25853343640992676
1103	98-99	0.15526822638079096
1103	100	0.046869062710761966
1104	1	0.07284722704309843
1104	2	0.14239784269576816
1104	3	0.17283927626316853
1104	4	0.15793141311390713
1104	5	0.1261496121250758
1104	6	0.1549960249459943
1104	7	0.1462567403547368
1104	8	0.1871133840046042
1104	9	0.21256273100799916
1104	10-11	0.26540662048423513
1104	12-13	0.2599641290444694
1104	14-15	0.353554977873479
1104	16-17	0.2977244354448203
1104	18-19	0.26985169094322714
1104	20-21	0.2830123799126554
1104	22-23	0.28119544829819176
1104	24-25	0.2507188461062242
1104	26-27	0.2633596704230996
1104	28-29	0.29815087436183774
1104	30-31	0.28036568396819916
1104	32-33	0.21135361251084817
1104	34-35	0.2696967912137467
1104	36-37	0.25198186812659884
1104	38-39	0.2790636898439587
1104	40-41	0.2971340650654497
1104	42-43	0.28675008669436153
1104	44-45	0.27903003863773534
1104	46-47	0.28314983986677333
1104	48-49	0.2604209616919846
1104	50-51	0.26230706132169246
1104	52-53	0.2084908005984829
1104	54-55	0.36061622408792005
1104	56-57	0.3422254667659601
1104	58-59	0.3350332334867616
1104	60-61	0.338997073251079
1104	62-63	0.3285005393773588
1104	64-65	0.30294148409074495
1104	66-67	0.25843284251899235
1104	68-69	0.29619535234093775
1104	70-71	0.2436748067858474
1104	72-73	0.2107383363435531
1104	74-75	0.26527863073948055
1104	76-77	0.21474034842398027
1104	78-79	0.2364011269524866
1104	80-81	0.22228454560798383
1104	82-83	0.23693742410350183
1104	84-85	0.21628875460606878
1104	86-87	0.21360103868646974
1104	88-89	0.18960105539863292
1104	90-91	0.21046823869095022
1104	92-93	0.22630331499787815
1104	94-95	0.1894045749046427
1104	96-97	0.1896924079394111
1104	98-99	0.14172018972979572
1104	100	0.1796556548453374
1105	1	0.06850077843611757
1105	2	0.15325514280662844
1105	3	0.1619077504896893
1105	4	0.13104562932249308
1105	5	0.09609635043237574
1105	6	0.1680909129566004
1105	7	0.14648016758566484
1105	8	0.18403242607196546
1105	9	0.22374389411759665
1105	10-11	0.30940414216510703
1105	12-13	0.26106102073654114
1105	14-15	0.3689662414084367
1105	16-17	0.27053544454089007
1105	18-19	0.3138190092895883
1105	20-21	0.29073108648098867
1105	22-23	0.2911981731326705
1105	24-25	0.27631422389246296
1105	26-27	0.2599972745280965
1105	28-29	0.31273687050349963
1105	30-31	0.32302174681204576
1105	32-33	0.3604650790543218
1105	34-35	0.303619008092042
1105	36-37	0.3377229997511577
1105	38-39	0.31097261730346304
1105	40-41	0.2836273178026687
1105	42-43	0.27782224238789865
1105	44-45	0.21966273460407137
1105	46-47	0.2610779545269608
1105	48-49	0.2549893590669967
1105	50-51	0.3002658280101542
1105	52-53	0.3076103879183165
1105	54-55	0.3133110984586196
1105	56-57	0.2957700519336939
1105	58-59	0.31060115600630667
1105	60-61	0.26834923520115694
1105	62-63	0.2645206077506401
1105	64-65	0.2831473851591966
1105	66-67	0.25950663398447205
1105	68-69	0.3016997648472355
1105	70-71	0.22993604079874075
1105	72-73	0.2680607197419249
1105	74-75	0.29696432457916444
1105	76-77	0.2561560295354255
1105	78-79	0.2121934326578625
1105	80-81	0.2313929201818965
1105	82-83	0.2100670807988081
1105	84-85	0.21993581934897577
1105	86-87	0.23384001966662282
1105	88-89	0.23235238701354888
1105	90-91	0.2266473276204266
1105	92-93	0.2404902786239802
1105	94-95	0.20216956166974853
1105	96-97	0.22861983525591967
1105	98-99	0.2655915427890889
1105	100	0.15552500567524064
1106	1	0.06097281414638189
1106	2	0.08133625843506209
1106	3	0.089687810333821
1106	4	0.16283660769030917
1106	5	0.08861369917013917
1106	6	0.10824611526957284
1106	7	0.08937788144763203
1106	8	0.11754577269758215
1106	9	0.11612552306668533
1106	10-11	0.17764410730892877
1106	12-13	0.1923660555494351
1106	14-15	0.24485646754075674
1106	16-17	0.20969124414096996
1106	18-19	0.19988785023520705
1106	20-21	0.17517953079333637
1106	22-23	0.17262377914825322
1106	24-25	0.19343010624709933
1106	26-27	0.21467909939890717
1106	28-29	0.21820618523918256
1106	30-31	0.24507097827377322
1106	32-33	0.20765152207977167
1106	34-35	0.17327834669034559
1106	36-37	0.1994833058391592
1106	38-39	0.1561872972931866
1106	40-41	0.19183450429186877
1106	42-43	0.14169895309607483
1106	44-45	0.12722142311162798
1106	46-47	0.17737393192815176
1106	48-49	0.17905610517957626
1106	50-51	0.19798938507126707
1106	52-53	0.22985255177118802
1106	54-55	0.25008831036858936
1106	56-57	0.19499787223972476
1106	58-59	0.2909447432491774
1106	60-61	0.2544559678098608
1106	62-63	0.2833536299368262
1106	64-65	0.2612380977775288
1106	66-67	0.2978377041671507
1106	68-69	0.2196299774756767
1106	70-71	0.18761280748454823
1106	72-73	0.23299103336094618
1106	74-75	0.20288204900185747
1106	76-77	0.18757125372007266
1106	78-79	0.23264744010808158
1106	80-81	0.1925831777655631
1106	82-83	0.17037702375355934
1106	84-85	0.2644373212230775
1106	86-87	0.1736544624727756
1106	88-89	0.11227521867380119
1106	90-91	0.16899811116300612
1106	92-93	0.1469854267483015
1106	94-95	0.19293314850865784
1106	96-97	0.1736891234455058
1106	98-99	0.13184739519279987
1106	100	0.053119918058705196
1107	1	0.08522510493407154
1107	2	0.12195838609699905
1107	3	0.17265050500564172
1107	4	0.19098809548712836
1107	5	0.13941482907911507
1107	6	0.1292927087384328
1107	7	0.06798131617095038
1107	8	0.09720841098505417
1107	9	0.20690574354892988
1107	10-11	0.20363829111078502
1107	12-13	0.1182131289796331
1107	14-15	0.12738766363884935
1107	16-17	0.15217561067809982
1107	18-19	0.15824220502329922
1107	20-21	0.14795692372558022
1107	22-23	0.19883212347606616
1107	24-25	0.188499392332389
1107	26-27	0.20840490482013507
1107	28-29	0.19400227944269943
1107	30-31	0.20634956416546402
1107	32-33	0.2015754337646598
1107	34-35	0.1715392201199819
1107	36-37	0.18191136580099254
1107	38-39	0.16353117236991466
1107	40-41	0.1307555912043128
1107	42-43	0.14090896117875928
1107	44-45	0.13616430271299862
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2216	48-49	-0.5330495181399186
2216	50-51	-0.5251887053120043
2216	52-53	-0.5112062923183913
2216	54-55	-0.4841277411586802
2216	56-57	-0.5212006483937088
2216	58-59	-0.493335911074702
2216	60-61	-0.6309909225936252
2216	62-63	-0.5609414516852169
2216	64-65	-0.5286649748664018
2216	66-67	-0.5707010497202418
2216	68-69	-0.6256485776047214
2216	70-71	-0.6732893323962372
2216	72-73	-0.5564961069304246
2216	74-75	-0.550181671565042
2216	76-77	-0.49970615172448873
2216	78-79	-0.5489992679538958
2216	80-81	-0.506722147406613
2216	82-83	-0.5191039430354465
2216	84-85	-0.4830549524239416
2216	86-87	-0.49040094744820095
2216	88-89	-0.47692427683397653
2216	90-91	-0.5002790626699323
2216	92-93	-0.5157589940436793
2216	94-95	-0.5222320137352767
2216	96-97	-0.5254572235129089
2216	98-99	-0.49095057420498023
2216	100	-0.4515117331426204
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	120.0
1	69.0
2	61.0
3	57.0
4	51.0
5	51.0
6	129.0
7	514.0
8	1674.0
9	2313.0
10	2519.0
11	3183.0
12	4447.0
13	5782.0
14	7516.0
15	8294.0
16	8874.0
17	9760.0
18	10859.0
19	12069.0
20	13778.0
21	15273.0
22	17394.0
23	20911.0
24	26078.0
25	32883.0
26	37909.0
27	37314.0
28	40469.0
29	50274.0
30	59918.0
31	69125.0
32	89840.0
33	122856.0
34	187107.0
35	301888.0
36	574396.0
37	1173288.0
38	1276759.0
39	382877.0
40	441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88930306189395	19.12295380564687	12.880218417040906	24.10752471541828
2	14.998170835700254	21.203073690368267	38.82992504115077	24.968830432780706
3	19.338670462472837	26.269915234469508	29.592099110466425	24.799315192591234
4	12.682390088585288	17.16125664129273	38.64385051986844	31.512502750253546
5	13.439830576512888	38.34449731695515	34.5418643961142	13.673807710417766
6	30.29077975095984	36.83358453178792	18.665600248580148	14.210035468672089
7	26.598170098004122	32.494624635766186	23.02070518703664	17.886500079193056
8	25.75919910954312	33.69461494900051	21.160740051249004	19.38544589020736
9	26.500517056799005	16.264918813620444	20.747799091212464	36.486765038368084
10-11	24.880261829202617	25.747601439505157	28.003673836564175	21.36846289472805
12-13	26.123485982502782	23.624119004167422	27.529347361715413	22.723047651614383
14-15	23.766786466479793	24.814303418531264	25.536270245985794	25.882639869003153
16-17	22.753232652234036	27.65025624490499	26.586393448705557	23.01011765415541
18-19	22.651340369343906	28.152638221893188	27.125281013338277	22.070740395424632
20-21	23.88484975251671	26.951626845709814	26.076810516238947	23.08671288553453
22-23	23.647816113102017	26.65779201463569	25.656112043223196	24.038279829039098
24-25	23.845377512385436	27.020390690573965	25.729714729510018	23.40451706753058
26-27	23.803307563576574	26.495809946470857	26.316287367177914	23.384595122774655
28-29	23.204904485236803	27.340140176193916	26.217830181873214	23.237125156696067
30-31	23.57652467516349	26.817577526692432	26.1304536222013	23.475444175942776
32-33	23.44766314491226	27.243044884928647	25.831750165802692	23.477541804356402
34-35	23.802210835716213	27.11721673558893	26.148272762898024	22.932299665796837
36-37	23.976987619300992	26.417127176126915	26.380248082699442	23.225637121872655
38-39	23.99134081052088	26.62506549347496	26.24657315462471	23.137020541379453
40-41	23.531332853329236	26.660690485459476	26.539778061035168	23.268198600176117
42-43	23.718004640612918	26.534240796247925	25.84616750129991	23.90158706183925
44-45	23.57659088433077	26.655079184503993	26.087829324157102	23.680500607008135
46-47	23.75385277149967	26.848635833498633	26.197826170936526	23.199685224065167
48-49	23.924468647535114	26.50988263931752	26.33383944530822	23.231809267839147
50-51	23.12379588600761	26.74410831920282	26.509932088893212	23.622163705896355
52-53	23.31160530587796	27.101625098747316	25.675073572178864	23.911696023195855
54-55	23.293539960656986	26.622980664581554	25.93713126777633	24.146348106985126
56-57	23.906843456557965	26.139691622622212	26.00423200081089	23.949232920008935
58-59	23.848510488863162	26.236043073428387	26.33037973735709	23.585066700351355
60-61	23.740781797947538	26.639178233675555	26.50463358613359	23.11540638224332
62-63	23.26226510486066	26.663027039262943	26.188301994448537	23.88640586142786
64-65	22.767257336199812	27.068179862383413	26.11391203255814	24.050650768858635
66-67	23.73949110182413	26.214615385450163	25.814737363005857	24.231156149719855
68-69	23.486971777411643	26.230897840303157	26.061090012799344	24.22104036948586
70-71	23.577240713274193	26.192651057123946	25.995789063350383	24.234319166251474
72-73	23.754742954003724	25.84105128765809	26.691329394459135	23.71287636387905
74-75	23.40422137697591	27.04220259193193	26.02981701679854	23.523759014293617
76-77	23.386414574991402	26.217963973601865	26.118612315069452	24.27700913633728
78-79	23.41108639596144	26.33552152299824	26.384193378218512	23.869198702821805
80-81	23.525120518146636	26.336682446350164	26.45492154653921	23.68327548896399
82-83	23.504469539834528	25.8085362887827	26.917491787856086	23.76950238352668
84-85	23.46149793419483	26.526386017831044	26.144597571950495	23.867518476023626
86-87	23.11370851480464	26.65053387425212	26.4109185002797	23.82483911066354
88-89	23.4033668887878	26.09405004149579	26.076090957389553	24.426492112326855
90-91	23.564184872364695	25.81739671223666	26.239525323430275	24.37889309196837
92-93	23.812296698616127	26.321077148042743	26.261924075495855	23.604702077845268
94-95	23.07255630464914	26.130618364672415	26.830967329130477	23.965858001547964
96-97	22.97442328297945	25.975917122816295	26.98184451781615	24.067815076388108
98-99	23.12050486865066	26.56754404345688	26.16892811989339	24.143022967999066
100	23.222410526153592	25.729811565131204	25.948701321390104	25.099076587325097
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1600.0
1	1109.0
2	648.5
3	662.0
4	618.0
5	508.5
6	394.5
7	406.0
8	465.0
9	455.0
10	389.5
11	323.0
12	329.0
13	369.0
14	365.0
15	398.5
16	458.0
17	531.0
18	671.5
19	843.0
20	1106.5
21	1636.5
22	2414.5
23	3510.5
24	5001.0
25	7335.5
26	11065.0
27	15388.0
28	20333.0
29	28189.5
30	38070.0
31	47246.5
32	58010.0
33	67320.0
34	75693.5
35	85297.5
36	94914.5
37	106927.5
38	112470.0
39	113402.5
40	113703.0
41	113519.5
42	117719.0
43	128816.5
44	146778.0
45	165635.5
46	178078.5
47	199822.0
48	245413.5
49	253015.5
50	235159.5
51	219904.0
52	193403.0
53	182309.0
54	173392.5
55	156036.5
56	142822.0
57	128298.0
58	112738.0
59	103922.0
60	90277.5
61	72798.5
62	57995.0
63	44965.5
64	32220.5
65	22085.0
66	16162.0
67	12241.5
68	9199.5
69	7135.5
70	5767.5
71	4574.5
72	3531.0
73	3274.0
74	2909.5
75	2349.0
76	1749.5
77	1101.5
78	656.5
79	368.0
80	254.0
81	202.5
82	148.5
83	106.0
84	80.5
85	65.0
86	58.5
87	56.5
88	41.0
89	33.0
90	36.0
91	30.5
92	19.0
93	9.0
94	7.5
95	8.0
96	7.0
97	5.5
98	6.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06261498941229562
2	0.00993682091158399
3	0.011390460651924878
4	0.009850036449474086
5	0.011932863540111778
6	0.011846079078001875
7	0.0028204950185718746
8	0.00542402888186899
9	0.007420071510396778
10-11	0.006812580275627452
12-13	0.005923039539000937
14-15	0.009969365084875205
16-17	0.009611379178671851
18-19	0.007658728781199014
20-21	0.007029541430902211
22-23	0.0024516610546047833
24-25	0.003872756621654459
26-27	0.004968410455791995
28-29	0.006020672058874579
30-31	0.009047280174957475
32-33	0.010403287395424723
34-35	0.011846079078001875
36-37	0.017747422501475336
38-39	0.01814880063873364
40-41	0.017920991425695143
42-43	0.024039296004443364
44-45	0.02764085118200437
46-47	0.030331169507411396
48-49	0.03132919082167529
50-51	0.032088554865136946
52-53	0.03037456173846634
54-55	0.036764067761308014
56-57	0.039985940917138196
58-59	0.03656880272156073
60-61	0.03748003957371472
62-63	0.03266350192661506
64-65	0.03489820182594508
66-67	0.03720883812962127
68-69	0.040202902072412956
70-71	0.039736435588572226
72-73	0.03531042802096712
74-75	0.038716718158780854
76-77	0.03793565799979172
78-79	0.037631912382407054
80-81	0.04187350296802861
82-83	0.03832618807928628
84-85	0.03459445620856042
86-87	0.03314081646821953
88-89	0.03089526851112577
90-91	0.03296724754399972
92-93	0.034334102822230705
94-95	0.038488908945742355
96-97	0.033216752872565695
98-99	0.030949508799944455
100	0.028660568611795743
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4609120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.516522754541555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88502350714668	39.18921519738116
2	14.114751735871907	15.389743683667314
3	5.692720278545999	9.310419438017792
4	2.7933252940246627	6.0912952781012795
5	1.6328556706315729	4.4508806661434175
6	1.0126615006777524	3.312407024660813
7	0.682966842186879	2.606308417487486
8	0.4526156708311638	1.9740026014343381
9	0.3308475378793289	1.6232991594374233
>10	1.356054696882625	11.782110737350799
>50	0.03471824731441591	1.2620194419528867
>100	0.010771811270410998	0.8849817933766497
>500	1.3744134733720934E-4	0.041023213258567164
>1k	2.7488269462883877E-4	0.36471322466584677
>5k	1.3744134731441939E-4	0.43615730473135983
>10k+	1.3744134731441939E-4	1.2814228183328837
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	20936	0.4542298746832367	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	13268	0.2878640608185511	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	6079	0.13189068629152637	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	5563	0.12069549067934876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.084805776373798E-4
2	0.0	0.0	0.0	0.0	1.084805776373798E-4
3	0.0	0.0	0.0	0.0	1.084805776373798E-4
4	0.0	0.0	0.0	0.0	1.084805776373798E-4
5	0.0	2.169611552747596E-5	0.0	0.0	1.518728086923317E-4
6	0.0	2.169611552747596E-5	0.0	0.0	2.169611552747596E-4
7	0.0	2.169611552747596E-5	0.0	2.169611552747596E-5	2.169611552747596E-4
8	0.0	2.169611552747596E-5	0.0	2.169611552747596E-5	2.169611552747596E-4
9	0.0	2.169611552747596E-5	0.0	4.339223105495192E-5	2.169611552747596E-4
10-11	0.0	2.169611552747596E-5	0.0	4.339223105495192E-5	2.2780921303849758E-4
12-13	0.0	2.169611552747596E-5	0.0	4.339223105495192E-5	8.244523900440865E-4
14-15	0.0	2.169611552747596E-5	0.0	5.424028881868991E-5	0.00107395771861006
16-17	0.0	2.169611552747596E-5	0.0	7.593640434616587E-5	0.0012909188738848196
18-19	0.0	2.169611552747596E-5	0.0	8.678446210990384E-5	0.0013451591627035096
20-21	0.0	2.169611552747596E-5	0.0	1.735689242198077E-4	0.001681448953379387
22-23	0.0	2.169611552747596E-5	0.0	4.5561842607699517E-4	0.0017790814732530288
24-25	0.0	2.169611552747596E-5	0.0	9.546290832089424E-4	0.0017790814732530288
26-27	0.0	2.169611552747596E-5	0.0	0.0018007775887805048	0.0018550178775991947
28-29	0.0	2.169611552747596E-5	0.0	0.005782014788072344	0.0018658659353629327
30-31	0.0	2.169611552747596E-5	0.0	0.01569713958412886	0.0018658659353629327
32-33	0.0	2.169611552747596E-5	0.0	0.03742579928489603	0.0018875620508904086
34-35	0.0	2.169611552747596E-5	0.0	0.0713476759121047	0.0019960426285277884
36-37	0.0	2.169611552747596E-5	0.0	0.12231185128614572	0.0019960426285277884
38-39	0.0	2.169611552747596E-5	0.0	0.19478772520567916	0.002006890686291526
40-41	0.0	2.169611552747596E-5	0.0	0.2729262852778839	0.0020177387440552643
42-43	0.0	2.169611552747596E-5	0.0	0.3445994029229007	0.0020285868018190024
44-45	0.0	2.169611552747596E-5	0.0	0.43186117957440895	0.0021262193216926442
46-47	0.0	2.169611552747596E-5	0.0	0.5402441246919152	0.002213003783802548
48-49	0.0	2.169611552747596E-5	0.0	0.6504495435137293	0.002234699899330024
50-51	0.0	2.169611552747596E-5	0.0	0.7613600860901863	0.002299788245912452
52-53	0.0	2.169611552747596E-5	0.0	0.8703939146735169	0.0024950532856597356
54-55	0.0	2.169611552747596E-5	0.0	0.9886919845870795	0.0025167494011872115
56-57	0.0	2.169611552747596E-5	0.0	1.1245313639046066	0.002646926094352067
58-59	0.0	2.169611552747596E-5	0.0	1.2697543131877669	0.0027445586142257087
60-61	0.0	2.169611552747596E-5	0.0	1.4215186413024612	0.002755406671989447
62-63	0.0	2.169611552747596E-5	0.0	1.5697681987017045	0.0030157600583191587
64-65	0.0	2.169611552747596E-5	0.0	1.7289851425000866	0.0030591522893741105
66-67	0.0	2.169611552747596E-5	0.0	1.9064376713993125	0.0030700003471378487
68-69	0.0	2.169611552747596E-5	0.0	2.09956564376714	0.0030808484049015864
70-71	0.0	2.169611552747596E-5	0.0	2.3067960912278265	0.0031133925781928005
72-73	0.0	2.169611552747596E-5	0.0	2.510522616030826	0.003189328982538966
74-75	0.0	2.169611552747596E-5	0.0	2.712708716631374	0.003254417329121394
76-77	0.0	2.169611552747596E-5	0.0	2.930418387891832	0.0032869615024126077
78-79	0.0	2.169611552747596E-5	0.0	3.1775913840386014	0.0033412017912312977
80-81	0.0	2.169611552747596E-5	0.0	3.433149928836741	0.0033737459645225122
82-83	0.0	2.169611552747596E-5	0.0	3.695521053910508	0.0034930745999236295
84-85	2.169611552747596E-5	2.169611552747596E-5	0.0	3.959248186204742	0.0035798590620335336
86-87	2.169611552747596E-5	2.169611552747596E-5	0.0	4.231990054500642	0.0036991876974346513
88	2.169611552747596E-5	2.169611552747596E-5	0.0	4.446250043392231	0.003818516332835769
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1715	0.0	26.31852	1
TACGCGT	180	5.680842E-6	20.884626	8
GTATAAG	2000	0.0	19.982197	1
GTGATCG	1225	0.0	19.946949	8
CATGGGG	10820	0.0	19.631495	4
GGGCTAT	2985	0.0	19.520515	6
GTATATA	1470	0.0	19.51043	1
TAGGCAT	2530	0.0	19.503006	5
TGATCGC	1275	0.0	18.796164	9
GGCTATT	3055	0.0	18.610577	7
ACGCGTA	205	1.7459482E-5	18.33772	9
CTATTGA	3075	0.0	18.184906	9
ATGGGGG	6605	0.0	17.502298	5
GCTATTG	3195	0.0	17.35483	8
TACCTGG	4370	0.0	17.313759	2
CTAGGCA	2885	0.0	17.266417	4
CTAACGC	980	0.0	17.26328	3
ATTTAGG	3460	0.0	17.121775	1
AGGGCTA	3655	0.0	16.842892	5
TTTAGGG	3510	0.0	16.73593	2
>>END_MODULE