FastQCFastQC Report
Mon 16 Jan 2017
ERR1146565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4609120
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT228800.49640712326865No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA125790.27291543722012007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114000.24733571701322596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97450.21142864581525322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50460.10947859895164369No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC48000.10414135453188463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15700.030.2733941
GAGTACT133600.022.65393312-13
GTACTTT140650.021.36805714-15
GTGATCG13400.021.0431488
CTATTGA31350.020.9872119
TAGTACT29550.020.8451124
CATGGGG105250.020.6847044
GGCTATT30950.020.4992227
CTAGTAC27100.020.3005123
ATTTAGG34100.020.1482731
TGATCGC13850.020.020119
TAGGCAT25700.019.9426945
GGGCTAT32200.019.7045126
AGTACTT136300.019.3778112-13
GCTATTG33400.019.1362428
CTGTGCG30500.019.1067189
ATGGGGG66250.018.9502895
CTAGGCA31300.018.7782924
ACTTTTT166000.018.28914316-17
GTATAAG23150.018.091661
Produced by FastQC (version 0.11.5)