##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146565_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4609120
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48757550248204	34.0	31.0	34.0	31.0	34.0
2	32.519346860138164	34.0	31.0	34.0	31.0	34.0
3	32.671100557156244	34.0	31.0	34.0	31.0	34.0
4	36.1506291873503	37.0	35.0	37.0	35.0	37.0
5	36.17386290658521	37.0	35.0	37.0	35.0	37.0
6	36.27314910438435	37.0	37.0	37.0	35.0	37.0
7	36.269613722359146	37.0	37.0	37.0	35.0	37.0
8	36.28627633908425	37.0	37.0	37.0	35.0	37.0
9	38.10388642517444	39.0	39.0	39.0	37.0	39.0
10-11	38.074258101329534	39.0	38.0	39.0	36.0	39.0
12-13	38.06191225223036	39.0	38.0	39.0	35.0	39.0
14-15	39.52337463550526	41.0	39.0	41.0	37.0	41.0
16-17	39.518702377026415	41.0	39.0	41.0	37.0	41.0
18-19	39.561600912972544	41.0	39.5	41.0	37.0	41.0
20-21	39.58842349515743	41.0	40.0	41.0	37.0	41.0
22-23	39.55227299354324	41.0	39.5	41.0	37.0	41.0
24-25	39.53927962387614	41.0	39.0	41.0	37.0	41.0
26-27	39.4636918761065	41.0	39.0	41.0	36.0	41.0
28-29	39.38362388048044	41.0	39.0	41.0	36.0	41.0
30-31	39.314284722463285	41.0	39.0	41.0	36.0	41.0
32-33	39.21388811747145	41.0	39.0	41.0	35.0	41.0
34-35	39.14579681153886	41.0	39.0	41.0	35.0	41.0
36-37	39.07135950029507	41.0	39.0	41.0	35.0	41.0
38-39	38.97608057503385	41.0	39.0	41.0	35.0	41.0
40-41	38.90271884871733	40.0	39.0	41.0	35.0	41.0
42-43	38.816604796577224	40.0	38.0	41.0	35.0	41.0
44-45	38.682710257055575	40.0	38.0	41.0	35.0	41.0
46-47	38.48034147516229	40.0	38.0	41.0	35.0	41.0
48-49	38.280237550768916	40.0	38.0	41.0	34.0	41.0
50-51	38.110242519179366	40.0	37.5	41.0	34.0	41.0
52-53	37.97931839483459	40.0	37.0	41.0	34.0	41.0
54-55	37.83031381261499	40.0	37.0	41.0	34.0	41.0
56-57	37.60214531190336	40.0	36.0	41.0	33.0	41.0
58-59	37.38196651421529	39.0	36.0	41.0	33.0	41.0
60-61	37.15428802027285	39.0	35.0	41.0	33.0	41.0
62-63	36.880093380081234	39.0	35.0	41.0	33.0	41.0
64-65	36.58117731801298	37.5	35.0	40.5	32.5	41.0
66-67	36.29377516749401	37.0	35.0	40.0	32.0	41.0
68-69	35.96487583313083	37.0	35.0	39.5	32.0	41.0
70-71	35.65524753098205	36.0	35.0	39.0	32.0	41.0
72-73	35.32949901499636	35.5	35.0	39.0	31.5	41.0
74-75	35.02130048686083	35.0	35.0	37.5	31.0	40.0
76-77	34.7209145129656	35.0	35.0	37.0	31.0	39.0
78-79	34.44734689051272	35.0	35.0	37.0	31.0	39.0
80-81	34.21031021105981	35.0	34.5	36.0	31.0	39.0
82-83	33.98316923837956	35.0	34.0	36.0	31.0	37.0
84-85	33.80541990661992	35.0	34.0	35.5	31.0	37.0
86-87	33.65691075519839	35.0	34.0	35.0	31.0	36.5
88-89	33.51822571336828	35.0	34.0	35.0	30.0	36.0
90-91	33.4379050664769	35.0	34.0	35.0	30.0	36.0
92-93	33.35703963012462	35.0	34.0	35.0	30.0	36.0
94-95	33.28757593640435	35.0	34.0	35.0	30.0	36.0
96-97	33.24682585829834	35.0	34.0	35.0	30.0	35.0
98-99	33.217440856389075	35.0	34.0	35.0	30.0	35.0
100	32.63320872530982	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2529191656286116
1101	2	0.212136547516927
1101	3	0.2217465491086017
1101	4	0.16164909567947916
1101	5	0.1511461465171564
1101	6	0.13236620022282608
1101	7	0.1303010227487178
1101	8	0.17997722617165834
1101	9	0.20813893513341952
1101	10-11	0.19294944119526747
1101	12-13	0.21682280592575864
1101	14-15	0.30568878540232447
1101	16-17	0.3225704780114995
1101	18-19	0.33381352447387513
1101	20-21	0.32498839457923623
1101	22-23	0.3065442394252358
1101	24-25	0.2921936903400848
1101	26-27	0.30252211205918655
1101	28-29	0.3354479331760132
1101	30-31	0.3369092508455509
1101	32-33	0.338144687944947
1101	34-35	0.34561563976171783
1101	36-37	0.3444896363612244
1101	38-39	0.3758056281425226
1101	40-41	0.3240974034740418
1101	42-43	0.2653891889906248
1101	44-45	0.28793119571098913
1101	46-47	0.2770586589402271
1101	48-49	0.2895091228882123
1101	50-51	0.2730558094772064
1101	52-53	0.2581717830943191
1101	54-55	0.30208211210025837
1101	56-57	0.28921691583253306
1101	58-59	0.2032761072045588
1101	60-61	0.22508922926813568
1101	62-63	0.23136765676171933
1101	64-65	0.22591770765489372
1101	66-67	0.23046258429274502
1101	68-69	0.254172556967859
1101	70-71	0.19301059661398057
1101	72-73	0.21794018006293214
1101	74-75	0.18815170869275732
1101	76-77	0.21477323528351633
1101	78-79	0.176919247801834
1101	80-81	0.1784746166620863
1101	82-83	0.2162507400117022
1101	84-85	0.22445237251851324
1101	86-87	0.21954565647345703
1101	88-89	0.20670858057854247
1101	90-91	0.2282896770391929
1101	92-93	0.21393906486557057
1101	94-95	0.219592742415486
1101	96-97	0.2050213711168567
1101	98-99	0.22238887500606097
1101	100	0.24162552419458194
1102	1	0.2521874402418405
1102	2	0.26206541832995356
1102	3	0.2730055391426376
1102	4	0.14792690452610202
1102	5	0.14922770752827574
1102	6	0.14908917892277174
1102	7	0.16745623426016465
1102	8	0.19139105774490872
1102	9	0.20589062041342032
1102	10-11	0.20721272101219768
1102	12-13	0.20363737986352248
1102	14-15	0.2947126905624913
1102	16-17	0.30431886676018394
1102	18-19	0.27616281044802093
1102	20-21	0.2960994967483117
1102	22-23	0.28414232687087804
1102	24-25	0.29590549864126814
1102	26-27	0.3192233872818804
1102	28-29	0.36360049666346583
1102	30-31	0.3714719741516461
1102	32-33	0.3512929284307802
1102	34-35	0.3456717891259302
1102	36-37	0.2935634900551847
1102	38-39	0.34690403070952414
1102	40-41	0.379673801597491
1102	42-43	0.3224751702623365
1102	44-45	0.3163656332875604
1102	46-47	0.3615486263687515
1102	48-49	0.32914931833308714
1102	50-51	0.3092851343823426
1102	52-53	0.37686938439347983
1102	54-55	0.3601215623724485
1102	56-57	0.3299071400612732
1102	58-59	0.35963650455997964
1102	60-61	0.31484342433196844
1102	62-63	0.2950955869937033
1102	64-65	0.3106916039396097
1102	66-67	0.3175168724946076
1102	68-69	0.304193735337968
1102	70-71	0.3537963772148558
1102	72-73	0.29821506394861075
1102	74-75	0.33087608668908075
1102	76-77	0.29838909908990274
1102	78-79	0.3367913847835666
1102	80-81	0.26661950102835874
1102	82-83	0.33847754645732664
1102	84-85	0.31910236406542936
1102	86-87	0.28828888787093376
1102	88-89	0.2833995368751303
1102	90-91	0.23228117623948918
1102	92-93	0.24024601944162782
1102	94-95	0.27847023800144655
1102	96-97	0.23678511639560185
1102	98-99	0.2602377130980642
1102	100	0.24898244648226608
1103	1	0.2135078343550063
1103	2	0.2866411819124295
1103	3	0.25582046754494314
1103	4	0.1936068110306408
1103	5	0.17690040329706846
1103	6	0.13612404171697534
1103	7	0.11448773183715844
1103	8	0.15408030248183024
1103	9	0.1645933477196806
1103	10-11	0.20023530681576318
1103	12-13	0.20770084419596913
1103	14-15	0.2919829770713136
1103	16-17	0.2649965054780097
1103	18-19	0.2528614211427467
1103	20-21	0.23908087472569406
1103	22-23	0.23891792694152514
1103	24-25	0.2745091210642272
1103	26-27	0.25614020439598306
1103	28-29	0.3064888431785491
1103	30-31	0.29551592218717104
1103	32-33	0.28677186762075735
1103	34-35	0.2977187661840901
1103	36-37	0.28271363612888933
1103	38-39	0.31780205816717455
1103	40-41	0.3579970755830715
1103	42-43	0.3053032389327228
1103	44-45	0.2843339807262808
1103	46-47	0.3194046106117696
1103	48-49	0.3126578184371809
1103	50-51	0.3532593277682139
1103	52-53	0.3151995845543851
1103	54-55	0.2601371262293881
1103	56-57	0.2889117527588283
1103	58-59	0.3335492148495902
1103	60-61	0.32379756286076145
1103	62-63	0.236916941014357
1103	64-65	0.26356558639691485
1103	66-67	0.32671056192720016
1103	68-69	0.3224361895867247
1103	70-71	0.24694401093474028
1103	72-73	0.25337590287458056
1103	74-75	0.2556414545256729
1103	76-77	0.22166172429382414
1103	78-79	0.21411744055299664
1103	80-81	0.23870980334966418
1103	82-83	0.2109446745909409
1103	84-85	0.19912415128419525
1103	86-87	0.20918894498203144
1103	88-89	0.2561798259495802
1103	90-91	0.2770137430226498
1103	92-93	0.21102234947871068
1103	94-95	0.20891477790519275
1103	96-97	0.23076139395885065
1103	98-99	0.22659849049922087
1103	100	0.23593451468276072
1104	1	0.16903143893723183
1104	2	0.191013558700881
1104	3	0.18850485586047938
1104	4	0.1485040479730344
1104	5	0.1031897701723139
1104	6	0.08887450272145969
1104	7	0.13889953666956956
1104	8	0.1498291949863102
1104	9	0.14177089455561998
1104	10-11	0.16094859931986605
1104	12-13	0.1747681575333857
1104	14-15	0.23082164720838705
1104	16-17	0.24614495279434578
1104	18-19	0.2357426903099551
1104	20-21	0.18185083740249297
1104	22-23	0.2100626350127328
1104	24-25	0.20265581926835807
1104	26-27	0.2173577826453652
1104	28-29	0.25214579032063256
1104	30-31	0.2520099468693573
1104	32-33	0.19534276278091056
1104	34-35	0.2588710552151383
1104	36-37	0.2627490236518355
1104	38-39	0.25489551300097446
1104	40-41	0.24341419076706927
1104	42-43	0.2867299895276787
1104	44-45	0.3193019150318861
1104	46-47	0.3176603152348605
1104	48-49	0.3106453261949085
1104	50-51	0.31099751452742197
1104	52-53	0.28673630902419944
1104	54-55	0.2770864883971669
1104	56-57	0.29060284572768325
1104	58-59	0.2778905650771364
1104	60-61	0.2369351641430626
1104	62-63	0.24895845770002722
1104	64-65	0.25262090302573625
1104	66-67	0.1838206485185907
1104	68-69	0.21915350135132883
1104	70-71	0.22165830161005573
1104	72-73	0.16182052536452574
1104	74-75	0.18204011376366225
1104	76-77	0.134821046059713
1104	78-79	0.1590329398224526
1104	80-81	0.18955472632845272
1104	82-83	0.18305375935696588
1104	84-85	0.186812955629577
1104	86-87	0.1574079381548259
1104	88-89	0.16670304761245092
1104	90-91	0.1630574941583518
1104	92-93	0.15026228638505046
1104	94-95	0.22031068515789798
1104	96-97	0.14454786274662723
1104	98-99	0.10833383039297928
1104	100	0.16552273749925206
1105	1	0.16655629019488316
1105	2	0.1179102839406383
1105	3	0.14696796208805551
1105	4	0.13999258857864305
1105	5	0.11286822058443136
1105	6	0.08931191864129318
1105	7	0.14823316567935052
1105	8	0.13100445852311537
1105	9	0.09845972429040017
1105	10-11	0.1351952227601103
1105	12-13	0.16373245687738347
1105	14-15	0.22692820021629245
1105	16-17	0.2064545403612783
1105	18-19	0.21316802109637223
1105	20-21	0.1718769364233026
1105	22-23	0.24505150394649888
1105	24-25	0.22194879212133856
1105	26-27	0.2242268268194607
1105	28-29	0.24362034252291664
1105	30-31	0.22368641901145736
1105	32-33	0.2525896378430019
1105	34-35	0.24768001793849237
1105	36-37	0.24813465138058177
1105	38-39	0.2596731335692155
1105	40-41	0.2314886776110967
1105	42-43	0.2662614583649656
1105	44-45	0.2904741633175263
1105	46-47	0.2737910125173144
1105	48-49	0.30124029098443117
1105	50-51	0.3156739369840764
1105	52-53	0.30979809171164163
1105	54-55	0.26259933073157526
1105	56-57	0.2991159266151868
1105	58-59	0.29869044956874546
1105	60-61	0.3155701384766445
1105	62-63	0.2851965660748661
1105	64-65	0.33867273209296656
1105	66-67	0.29750752430575744
1105	68-69	0.31159939811580273
1105	70-71	0.2438963415917712
1105	72-73	0.2111599714203649
1105	74-75	0.20760845859744848
1105	76-77	0.193646420471552
1105	78-79	0.23168563310679247
1105	80-81	0.2603390239149306
1105	82-83	0.2760449497364448
1105	84-85	0.27972979933949205
1105	86-87	0.2739558417445238
1105	88-89	0.267177694950405
1105	90-91	0.2554508376047977
1105	92-93	0.23208131657194997
1105	94-95	0.20752593795799612
1105	96-97	0.17706037286856002
1105	98-99	0.13703965550536168
1105	100	0.2743796935147529
1106	1	0.1316725303447015
1106	2	0.13230149833785276
1106	3	0.10136620542916575
1106	4	0.1186935035532386
1106	5	0.031398305676205496
1106	6	0.039721322883472965
1106	7	0.07358631240133917
1106	8	0.09153138693810803
1106	9	0.11687017883453876
1106	10-11	0.1048808073656815
1106	12-13	0.12430592174852961
1106	14-15	0.18850087159007956
1106	16-17	0.14501912383287419
1106	18-19	0.1328580088909348
1106	20-21	0.14447648571679395
1106	22-23	0.16403753550749656
1106	24-25	0.14853641298541476
1106	26-27	0.15670975796986397
1106	28-29	0.1854117537074771
1106	30-31	0.20247134660122157
1106	32-33	0.18786572587215744
1106	34-35	0.18856537632517956
1106	36-37	0.21285372734569563
1106	38-39	0.20767472633482953
1106	40-41	0.17014409488970728
1106	42-43	0.15065645269505978
1106	44-45	0.15111774450095794
1106	46-47	0.18396454894750747
1106	48-49	0.23214662655590956
1106	50-51	0.19628342374998198
1106	52-53	0.2187871529898402
1106	54-55	0.2495058048044072
1106	56-57	0.20559023028518908
1106	58-59	0.23076822962281796
1106	60-61	0.22157214097543942
1106	62-63	0.22347582520801978
1106	64-65	0.2418442515971364
1106	66-67	0.19857875151434712
1106	68-69	0.2230520522923385
1106	70-71	0.19685819889497225
1106	72-73	0.16935601432115988
1106	74-75	0.18242129421076925
1106	76-77	0.20005719818553303
1106	78-79	0.13742967682705398
1106	80-81	0.17363891686492394
1106	82-83	0.20378942627345964
1106	84-85	0.22531139250907017
1106	86-87	0.16339055422436388
1106	88-89	0.13724310798742323
1106	90-91	0.14457926587407144
1106	92-93	0.11306810411952029
1106	94-95	0.08665618405760256
1106	96-97	0.09005413165112941
1106	98-99	0.07734683997473724
1106	100	0.1575869463691788
1107	1	0.08918073107297175
1107	2	0.10299208159642603
1107	3	0.13092484653863323
1107	4	0.060777946750867784
1107	5	0.0227729116107227
1107	6	0.058971905475431186
1107	7	0.07935926957978978
1107	8	0.058903370861628446
1107	9	0.1059369706763107
1107	10-11	0.11354403171705485
1107	12-13	0.08996294382174597
1107	14-15	0.13269544480502304
1107	16-17	0.1441837911235666
1107	18-19	0.1219786075093765
1107	20-21	0.1814574066981649
1107	22-23	0.18765956595504463
1107	24-25	0.17588447661362494
1107	26-27	0.17343523286216112
1107	28-29	0.18668501780565094
1107	30-31	0.18138284539715244
1107	32-33	0.13741607876039552
1107	34-35	0.14070230973887732
1107	36-37	0.16138328468690588
1107	38-39	0.18946864849110057
1107	40-41	0.11989535381607652
1107	42-43	0.14199481710460304
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2216	38-39	-0.39253610761438296
2216	40-41	-0.417743055508339
2216	42-43	-0.43884960394871086
2216	44-45	-0.42415779120617714
2216	46-47	-0.41830753574654267
2216	48-49	-0.4068236787292605
2216	50-51	-0.38819441207107275
2216	52-53	-0.3709473737749889
2216	54-55	-0.4164834694721762
2216	56-57	-0.4042808883778619
2216	58-59	-0.44170262416076866
2216	60-61	-0.4240423014827641
2216	62-63	-0.4280642278099833
2216	64-65	-0.36214527113555306
2216	66-67	-0.3609547995339
2216	68-69	-0.3967797724071431
2216	70-71	-0.5028528508741559
2216	72-73	-0.4481529227289087
2216	74-75	-0.4254992756380318
2216	76-77	-0.4076248032757235
2216	78-79	-0.4392664129371866
2216	80-81	-0.42411044146561494
2216	82-83	-0.4142617093604173
2216	84-85	-0.4353315276501917
2216	86-87	-0.3941939471684677
2216	88-89	-0.3934328820995958
2216	90-91	-0.47295821414942196
2216	92-93	-0.4525602097689614
2216	94-95	-0.4618979656856297
2216	96-97	-0.45289085637876525
2216	98-99	-0.4420212814234361
2216	100	-0.5484484565192318
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	22.0
8	106.0
9	167.0
10	226.0
11	286.0
12	200.0
13	224.0
14	381.0
15	575.0
16	1151.0
17	2173.0
18	4031.0
19	6367.0
20	9225.0
21	13839.0
22	20300.0
23	25247.0
24	25525.0
25	22786.0
26	20671.0
27	22755.0
28	28098.0
29	34973.0
30	44274.0
31	57011.0
32	74911.0
33	101263.0
34	150819.0
35	251609.0
36	494451.0
37	1198848.0
38	1501037.0
39	494546.0
40	1020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56588598966817	19.27896316293194	13.175114990400019	23.98003585699987
2	15.184458649290796	21.397694055171918	38.45557545044703	24.96227184509026
3	19.19146072271779	26.40733487781351	29.98210931326753	24.419095086201175
4	12.878575519838927	17.500130176693165	38.151686222098796	31.46960808136911
5	13.128095780611506	38.31595612073851	34.57005751116874	13.985890587481245
6	30.09126013010009	37.131792246892054	18.217983285863717	14.558964337144136
7	26.106290594796967	32.91980528166012	22.927765588174225	18.046138535368687
8	25.912039608428504	33.31783507480821	21.00778456625126	19.762340750512028
9	26.577068941576698	15.610311729787899	20.878801159440414	36.933818169194986
10-11	24.87026807720346	25.510032283819907	27.96215980490853	21.65753983406811
12-13	26.513206425521574	23.404185614607563	27.806707137154163	22.2759008227167
14-15	23.840927917228417	24.43920941056402	26.334463556801975	25.385399115405594
16-17	22.280413614746415	27.28295206026313	27.331453726524803	23.10518059846565
18-19	22.148924306592146	27.202470319713957	28.816791491651333	21.831813882042557
20-21	23.330950376644566	25.9911978859305	28.010845888152186	22.667005849272744
22-23	23.025020725308785	25.749262491432706	27.636247344654564	23.589469438603945
24-25	23.29868825285521	26.134609209567117	27.65551775610095	22.911184781476724
26-27	23.25324573888291	25.61945664248273	28.266740722741	22.860556895893357
28-29	22.583925474694837	26.43625603938627	28.237728294713154	22.742090191205737
30-31	23.09477400418996	25.864233717904884	28.05409154735229	22.98690073055287
32-33	23.00677764324778	26.308390415770017	27.725330846342427	22.959501094639776
34-35	23.29541207729935	26.192288094655137	28.065009231257896	22.447290596787617
36-37	23.526688245768305	25.561537214837898	28.148013631327146	22.76376090806665
38-39	23.515822901873676	25.744069686572928	28.04935980375321	22.690747607800187
40-41	23.068067603843083	25.852790944786435	28.189092889381396	22.890048561989083
42-43	23.400697598910675	25.742078895956915	27.39968178138205	23.457541723750357
44-45	23.32418476492157	25.838276453103642	27.510826408626414	23.326712373348375
46-47	23.561941994614358	26.144872113499655	27.39592803941501	22.897257852470972
48-49	23.849349871666643	25.991627414522174	27.20837518902864	22.95064752478255
50-51	22.96259790307689	26.410732365296248	27.208319913284335	23.41834981834253
52-53	23.1747028057429	26.72229906958343	26.382913495786404	23.72008462888727
54-55	23.12915052453257	26.32177825567752	26.604949039456237	23.944122180333675
56-57	23.769319490500916	25.8102925618931	26.621583154627952	23.79880479297803
58-59	23.656494189092875	25.895404756558086	26.989621404185936	23.458479650163106
60-61	23.52245994960987	26.318477556322765	27.210507616828895	22.948554877238468
62-63	23.031755500642156	26.335259641548507	26.883080999649493	23.74990385815984
64-65	22.610097188786128	26.741415254539756	26.752003426401842	23.896484130272267
66-67	23.54757475204877	25.8945017602278	26.5290104266281	24.028913061095324
68-69	23.32760896992919	25.89717191858024	26.718789834138107	24.05642927735246
70-71	23.440892946311244	25.88437568513209	26.607083250118546	24.06764811843812
72-73	23.627047072857764	25.531461099811715	27.262124264845955	23.579367562484567
74-75	23.33094667777546	26.76179658751649	26.439023120183297	23.468233614524753
76-77	23.35280555821945	25.941536026147343	26.4636713981772	24.241987017456008
78-79	23.246878366652126	26.16394293401587	26.724585603803618	23.864593095528384
80-81	23.32450084278579	26.202675608939586	26.796238066320267	23.67658548195436
82-83	23.371606777354607	25.691835784491335	27.1853223851699	23.75123505298415
84-85	23.3551542133608	26.326221885209044	26.452992356996756	23.8656315444334
86-87	23.017549987850174	26.480423594959557	26.665220258964833	23.836806158225432
88-89	23.19075527013064	25.99398474218193	26.379156507896045	24.43610347979139
90-91	23.341071614538134	25.672698909987158	26.52518268129274	24.46104679418197
92-93	23.572395598292083	26.198905647932797	26.471951261846076	23.756747491929044
94-95	22.869137709584475	26.043322803485264	27.09161401742632	23.99592546950394
96-97	22.757493085146024	25.91236097202935	27.182836907730024	24.1473090350946
98-99	22.932441877632908	26.422262700200832	26.36601516069168	24.27928026147458
100	22.925604643705313	25.6875019255478	26.22693736144192	25.159956069304968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	367.0
1	258.0
2	329.0
3	491.0
4	311.0
5	215.0
6	465.0
7	1125.5
8	2226.0
9	2731.0
10	2023.5
11	1328.5
12	1291.5
13	1480.0
14	1730.5
15	2082.0
16	2534.5
17	3045.0
18	3571.0
19	4108.5
20	4740.5
21	5621.5
22	6613.0
23	7823.0
24	9446.0
25	11625.0
26	15387.5
27	19675.5
28	23934.0
29	31199.0
30	40483.5
31	48657.0
32	58482.0
33	66679.0
34	74023.5
35	83432.0
36	93129.0
37	104929.5
38	110102.0
39	110900.5
40	111536.0
41	111647.5
42	115690.5
43	126545.0
44	144511.5
45	163762.0
46	175304.5
47	197386.0
48	245567.0
49	252073.0
50	234103.5
51	218112.0
52	188292.5
53	177007.5
54	170188.5
55	154146.0
56	140603.5
57	126988.0
58	112165.0
59	102993.0
60	89452.5
61	72193.5
62	57089.5
63	43756.0
64	30977.5
65	21078.5
66	15407.0
67	11710.0
68	8836.0
69	6869.5
70	5444.0
71	4200.5
72	3256.0
73	3149.0
74	2721.5
75	2148.0
76	1592.5
77	914.5
78	527.0
79	274.5
80	144.5
81	107.5
82	77.0
83	44.5
84	35.5
85	29.5
86	20.5
87	12.5
88	5.5
89	2.5
90	2.5
91	2.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005575901690561322
2	0.08257541569757351
3	3.9053007949456733E-4
4	0.0
5	0.03994254868608324
6	0.004491095914187524
7	2.169611552747596E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.339223105495192E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.5561842607699517E-4
24-25	0.0
26-27	0.0
28-29	2.169611552747596E-5
30-31	1.7356892421980768E-4
32-33	0.0015078800291595793
34-35	0.001117349949665012
36-37	6.725795813517548E-4
38-39	5.532509459506371E-4
40-41	0.0013560072204672475
42-43	5.315548304231611E-4
44-45	4.3392231054951915E-4
46-47	2.9289755962092547E-4
48-49	6.508834658242788E-4
50-51	7.268198701704447E-4
52-53	0.0012041344117749157
54-55	0.0013885513937584614
56-57	9.546290832089423E-4
58-59	0.0024950532856597356
60-61	0.0017899295310167666
62-63	9.654771409726803E-4
64-65	0.004415159509841358
66-67	0.004328375047731454
68-69	0.003579859062033533
70-71	0.0013777033359947235
72-73	0.004816537647099663
74-75	0.003471378484396153
76-77	0.0035147707154511054
78-79	7.485159856979207E-4
80-81	2.169611552747596E-5
82-83	1.3017669316485578E-4
84-85	5.4240288818689894E-5
86-87	0.0
88-89	1.627208664560697E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	4.447703683132572E-4
98-99	6.400354080605408E-4
100	9.112368521539903E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4609120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.714746431792015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.66675280745841	34.82416956924796
2	15.776691200459581	16.002217875279836
3	6.246352047555758	9.503464806464853
4	3.1499518255454886	6.389960324195991
5	1.8082473023469527	4.585240171224882
6	1.1188733653848602	3.404602740886737
7	0.7486003251381869	2.657555296769814
8	0.5166798778784574	2.096263119441218
9	0.3657143558257621	1.6692399739952715
>10	1.5406567681018513	12.611364961532557
>50	0.04236632238554169	1.4507110023728993
>100	0.01723840358295005	1.4593040960540995
>500	7.501593344504889E-4	0.2716351626706641
>1k	7.501593344475184E-4	0.728849642356939
>5k	1.500318668895037E-4	0.5627108810993572
>10k+	2.2504780033425552E-4	1.7827103764069219
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	22880	0.49640712326865	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	12579	0.27291543722012007	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11400	0.24733571701322596	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9745	0.21142864581525322	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5046	0.10947859895164369	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	4800	0.10414135453188463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7356892421980768E-4
2	0.0	0.0	0.0	0.0	1.7356892421980768E-4
3	0.0	0.0	0.0	0.0	1.7356892421980768E-4
4	0.0	0.0	0.0	0.0	1.7356892421980768E-4
5	0.0	0.0	0.0	0.0	2.169611552747596E-4
6	0.0	0.0	0.0	0.0	2.169611552747596E-4
7	0.0	0.0	0.0	2.169611552747596E-5	2.169611552747596E-4
8	0.0	0.0	0.0	2.169611552747596E-5	2.169611552747596E-4
9	0.0	0.0	0.0	4.339223105495192E-5	2.169611552747596E-4
10-11	0.0	0.0	0.0	4.339223105495192E-5	2.8204950185718746E-4
12-13	0.0	0.0	0.0	4.339223105495192E-5	6.617315235880167E-4
14-15	0.0	0.0	0.0	5.424028881868991E-5	8.244523900440865E-4
16-17	0.0	0.0	0.0	7.593640434616587E-5	9.980213142638942E-4
18-19	0.0	0.0	0.0	8.678446210990384E-5	0.0011281980074287499
20-21	0.0	0.0	0.0	1.735689242198077E-4	0.0013126149894122957
22-23	0.0	0.0	0.0	4.5561842607699517E-4	0.0013451591627035096
24-25	0.0	0.0	0.0	9.654771409726803E-4	0.0013451591627035096
26-27	0.0	0.0	0.0	0.0018116256465442427	0.0015512722602145312
28-29	0.0	0.0	0.0	0.005782014788072344	0.0015838164335057452
30-31	0.0	0.0	0.0	0.015773075988475025	0.0016055125490332211
32-33	0.0	0.0	0.0	0.037577672093588366	0.0017248411844343389
34-35	0.0	0.0	0.0	0.07173820599159926	0.0018875620508904086
36-37	0.0	0.0	0.0	0.12284340611656888	0.0018875620508904086
38-39	0.0	0.0	0.0	0.19535182420939357	0.0019418023397090984
40-41	0.0	0.0	0.0	0.27357716874370813	0.0019526503974728365
42-43	0.0	0.0	0.0	0.34554318394834593	0.0019526503974728365
44-45	0.0	0.0	0.0	0.43321718679487625	0.0020394348595827406
46-47	0.0	0.0	0.0	0.5419038775297671	0.00214791543722012
48-49	0.0	0.0	0.0	0.6529120526260979	0.002267244072621238
50-51	0.0	0.0	0.0	0.7640612524733572	0.0024299649390773074
52-53	0.0	0.0	0.0	0.8735832436560558	0.0026252299788245914
54-55	0.0	0.0	0.0	0.9921091227826571	0.002690318325407019
56-57	0.0	0.0	0.0	1.1283932724684973	0.002712014440934495
58-59	0.0	0.0	0.0	1.2743972819106468	0.002733710556461971
60-61	0.0	0.0	0.0	1.426454507584962	0.002777102787516923
62-63	0.0	0.0	0.0	1.5751922275835732	0.0027987989030443987
64-65	0.0	0.0	0.0	1.7347129169993405	0.002809646960808137
66-67	0.0	0.0	0.0	1.9128922657687366	0.0028313430763356132
68-69	0.0	0.0	0.0	2.1065084007359323	0.002842191134099351
70-71	0.0	0.0	0.0	2.315040615128267	0.002874735307390565
72-73	0.0	0.0	0.0	2.5198627937654043	0.0029615197695004687
74-75	0.0	0.0	0.0	2.7229167389870517	0.0031133925781928005
76-77	0.0	0.0	0.0	2.9416895199083557	0.0032110250980664423
78-79	0.0	0.0	0.0	3.1897737529072794	0.00327611344464887
80-81	0.0	0.0	0.0	3.446308622904155	0.003297809560176346
82-83	0.0	0.0	0.0	3.71015508383379	0.0035256187732148436
84-85	0.0	0.0	0.0	3.974446315131739	0.003569011004269796
86-87	0.0	0.0	0.0	4.248099420279793	0.0037642760440170795
88	0.0	0.0	0.0	4.463606935814211	0.003948693026000625
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1570	0.0	30.273394	1
GAGTACT	13360	0.0	22.653933	12-13
GTACTTT	14065	0.0	21.368057	14-15
GTGATCG	1340	0.0	21.043148	8
CTATTGA	3135	0.0	20.987211	9
TAGTACT	2955	0.0	20.845112	4
CATGGGG	10525	0.0	20.684704	4
GGCTATT	3095	0.0	20.499222	7
CTAGTAC	2710	0.0	20.300512	3
ATTTAGG	3410	0.0	20.148273	1
TGATCGC	1385	0.0	20.02011	9
TAGGCAT	2570	0.0	19.942694	5
GGGCTAT	3220	0.0	19.704512	6
AGTACTT	13630	0.0	19.37781	12-13
GCTATTG	3340	0.0	19.136242	8
CTGTGCG	3050	0.0	19.106718	9
ATGGGGG	6625	0.0	18.950289	5
CTAGGCA	3130	0.0	18.778292	4
ACTTTTT	16600	0.0	18.289143	16-17
GTATAAG	2315	0.0	18.09166	1
>>END_MODULE