FastQCFastQC Report
Mon 16 Jan 2017
ERR1146564_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146564_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4383839
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53250.036.467611
GTATCAA81950.032.8762661
CAACGCA82400.030.3975775
ATCAACG86450.029.1369273
TATTCGC4450.027.4560159
TATCAAC99800.026.6516972
GTACTAG23300.026.4357871
AACGCAG100000.026.3161326
GTACATG169050.025.5331461
TACATGG169900.025.1425572
ACATGGG170000.024.5198973
CGCAGAG104800.023.9444078
ACGCAGA104750.023.910437
TCAACGC108950.023.3353314
GCTATTG32750.022.3839868
CATGGGG133600.022.2307724
TAGGCAT39850.021.9342925
GTATAAG31700.021.5072841
CTATTGA34700.021.261529
GGGCTAT35700.021.061336
Produced by FastQC (version 0.11.5)