FastQCFastQC Report
Mon 16 Jan 2017
ERR1146564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4383839
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151230.34497161049938196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143620.3276123963494097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74710.17042140461818966No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT54560.12445712536432109No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94850.058.0454751
GTATCAA179000.042.6926231
CAACGCA220200.033.8423085
ATCAACG220200.033.8423083
TATCAAC231400.032.9417532
AACGCAG238500.031.8651586
GTGGTAT23000.031.3028181
TCAACGC244700.030.7037094
CGCAGAG258750.028.7703068
ACGCAGA259750.028.6595467
GCAGAGT289450.025.5402329
GTACATG167500.024.0198971
GTACTAG23550.023.5782171
GAGTACT191050.023.40622512-13
TACATGG168400.023.387212
TATTCGC3650.023.1765799
ACATGGG163700.023.005513
CAGAGTA279650.022.70440910-11
AGAGTAC268200.022.63108310-11
AGTACTT201150.021.35481512-13
Produced by FastQC (version 0.11.5)