##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146564_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4383839
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44757277810613	34.0	31.0	34.0	31.0	34.0
2	32.537671889866395	34.0	31.0	34.0	31.0	34.0
3	32.701551767754246	34.0	31.0	34.0	31.0	34.0
4	36.19890648356384	37.0	37.0	37.0	35.0	37.0
5	36.20611751480837	37.0	37.0	37.0	35.0	37.0
6	36.29159100049067	37.0	37.0	37.0	35.0	37.0
7	36.28271635888088	37.0	37.0	37.0	35.0	37.0
8	36.295638822502376	37.0	37.0	37.0	35.0	37.0
9	38.110848048936106	39.0	39.0	39.0	37.0	39.0
10-11	38.07878676657605	39.0	38.0	39.0	36.0	39.0
12-13	38.051049434069085	39.0	38.0	39.0	35.0	39.0
14-15	39.50317872075138	41.0	39.0	41.0	37.0	41.0
16-17	39.49655176661369	41.0	39.0	41.0	36.5	41.0
18-19	39.54514593259469	41.0	39.5	41.0	37.0	41.0
20-21	39.575943710524044	41.0	40.0	41.0	37.0	41.0
22-23	39.532001175225645	41.0	39.5	41.0	36.5	41.0
24-25	39.508071122137466	41.0	39.0	41.0	36.0	41.0
26-27	39.427856953688305	41.0	39.0	41.0	36.0	41.0
28-29	39.34221820190021	41.0	39.0	41.0	36.0	41.0
30-31	39.231514661008305	41.0	39.0	41.0	35.5	41.0
32-33	39.09704747368687	41.0	39.0	41.0	35.0	41.0
34-35	39.00880894576648	41.0	39.0	41.0	35.0	41.0
36-37	38.91582252450421	41.0	38.5	41.0	35.0	41.0
38-39	38.79812693851211	40.0	38.0	41.0	35.0	41.0
40-41	38.69240122641365	40.0	38.0	41.0	35.0	41.0
42-43	38.572751189083355	40.0	38.0	41.0	35.0	41.0
44-45	38.389495941798955	40.0	38.0	41.0	34.5	41.0
46-47	38.1101080582567	40.0	38.0	41.0	34.0	41.0
48-49	37.8397720582348	40.0	37.0	41.0	33.0	41.0
50-51	37.649247497455995	40.0	37.0	41.0	33.0	41.0
52-53	37.49801749106206	40.0	36.5	41.0	33.0	41.0
54-55	37.33646685017401	40.0	36.0	41.0	33.0	41.0
56-57	37.097904028866026	39.5	35.5	41.0	32.5	41.0
58-59	36.84880614913094	39.0	35.0	41.0	32.0	41.0
60-61	36.640316170370305	39.0	35.0	41.0	32.0	41.0
62-63	36.35762102577216	38.0	35.0	41.0	32.0	41.0
64-65	36.04963070039753	37.0	35.0	40.0	31.0	41.0
66-67	35.7831546733354	37.0	35.0	40.0	31.0	41.0
68-69	35.475904224584895	36.0	35.0	39.5	31.0	41.0
70-71	35.216441114739844	36.0	35.0	39.0	31.0	41.0
72-73	34.91915339500379	35.0	35.0	39.0	31.0	41.0
74-75	34.61463160485593	35.0	35.0	37.5	30.0	40.0
76-77	34.34395378114935	35.0	35.0	37.0	30.0	39.0
78-79	34.09687981242012	35.0	35.0	37.0	30.0	39.0
80-81	33.882011634095136	35.0	34.5	36.0	30.0	39.0
82-83	33.663084570395945	35.0	34.0	36.0	30.0	37.0
84-85	33.48421166926979	35.0	34.0	35.5	30.0	37.0
86-87	33.325575026820104	35.0	34.0	35.0	29.5	37.0
88-89	33.20481967973733	35.0	34.0	35.0	29.0	36.0
90-91	33.130137420648886	35.0	34.0	35.0	29.0	36.0
92-93	33.053589901453954	35.0	34.0	35.0	29.0	36.0
94-95	32.991124331892664	35.0	34.0	35.0	29.0	36.0
96-97	32.94561650188339	35.0	34.0	35.0	29.0	35.0
98-99	32.900856532368095	35.0	34.0	35.0	29.0	35.0
100	32.352458427419435	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19771981247723858
1101	2	0.20706651925671338
1101	3	0.22153721815992355
1101	4	0.1537061419721084
1101	5	0.1576676276320228
1101	6	0.1330339649643193
1101	7	0.15981395409100685
1101	8	0.15399929577161942
1101	9	0.2029969850282427
1101	10-11	0.2177033541606761
1101	12-13	0.2306142454598188
1101	14-15	0.312145724079933
1101	16-17	0.32974710835775767
1101	18-19	0.2982695011336034
1101	20-21	0.2704388510954914
1101	22-23	0.2998288306698669
1101	24-25	0.29928112120386174
1101	26-27	0.33085191731411356
1101	28-29	0.3442429719544009
1101	30-31	0.38207009906584233
1101	32-33	0.39926054906590025
1101	34-35	0.4036155981258389
1101	36-37	0.3544644649802322
1101	38-39	0.3896569286589724
1101	40-41	0.38037375146971897
1101	42-43	0.35523529938538445
1101	44-45	0.4497358041267958
1101	46-47	0.42453079529453674
1101	48-49	0.4793850388597818
1101	50-51	0.4726237145358354
1101	52-53	0.47431237659324665
1101	54-55	0.45053710648819134
1101	56-57	0.4869400696282682
1101	58-59	0.5034183656084892
1101	60-61	0.4670492466904932
1101	62-63	0.42616664111587
1101	64-65	0.43126688627256726
1101	66-67	0.42746332144684374
1101	68-69	0.43708412361316107
1101	70-71	0.35068682488289227
1101	72-73	0.3616436059528212
1101	74-75	0.39028265423782216
1101	76-77	0.38786292850294757
1101	78-79	0.3560114219602326
1101	80-81	0.44434102155526745
1101	82-83	0.3935144535135393
1101	84-85	0.39432795649086927
1101	86-87	0.34762844803670134
1101	88-89	0.39030393143539044
1101	90-91	0.3645348102984798
1101	92-93	0.34163623785995867
1101	94-95	0.36434411959473323
1101	96-97	0.3319626944727361
1101	98-99	0.31523379343020963
1101	100	0.3683442579987357
1102	1	0.17182534525177573
1102	2	0.180878615373409
1102	3	0.19464484038419982
1102	4	0.14156541989201799
1102	5	0.08999947763342675
1102	6	0.13006470728627306
1102	7	0.12634297244121484
1102	8	0.12766002977995328
1102	9	0.1653796103651004
1102	10-11	0.1825260973340619
1102	12-13	0.18717859167591655
1102	14-15	0.2532314748560722
1102	16-17	0.20859397759695497
1102	18-19	0.22608282822837822
1102	20-21	0.25453911506297544
1102	22-23	0.2983640320241392
1102	24-25	0.23598527781319945
1102	26-27	0.23247583443495046
1102	28-29	0.2806718277472271
1102	30-31	0.3131890440794791
1102	32-33	0.2646903471694273
1102	34-35	0.30733994689224176
1102	36-37	0.3236168421936796
1102	38-39	0.3313285486903794
1102	40-41	0.3106148966608657
1102	42-43	0.28550091854928183
1102	44-45	0.36707740857518445
1102	46-47	0.3323122384506618
1102	48-49	0.4519007047124859
1102	50-51	0.39905670956773776
1102	52-53	0.4244310941871916
1102	54-55	0.42522386473854823
1102	56-57	0.38998184557018334
1102	58-59	0.36590952987679515
1102	60-61	0.3275737076104477
1102	62-63	0.3545080412379633
1102	64-65	0.42080746336633723
1102	66-67	0.36290016554437443
1102	68-69	0.3920748561354799
1102	70-71	0.3127972870920672
1102	72-73	0.394633454399937
1102	74-75	0.3451161257968067
1102	76-77	0.2986468888385474
1102	78-79	0.3220391003918124
1102	80-81	0.3104216390725014
1102	82-83	0.3472411994741975
1102	84-85	0.30105701881892344
1102	86-87	0.2909775392616183
1102	88-89	0.3369981127044426
1102	90-91	0.29517870922573763
1102	92-93	0.3091409519236308
1102	94-95	0.3710702575968128
1102	96-97	0.3099189244668352
1102	98-99	0.34609478277110384
1102	100	0.28413044734846693
1103	1	0.14907048633546083
1103	2	0.1599228994332833
1103	3	0.15848502672717757
1103	4	0.16515896083821247
1103	5	0.09754564870445392
1103	6	0.06920728629143724
1103	7	0.14059263570690206
1103	8	0.130451514798267
1103	9	0.18794911552553373
1103	10-11	0.15239437126593458
1103	12-13	0.18826386039889087
1103	14-15	0.22864050213355114
1103	16-17	0.25557295805637636
1103	18-19	0.2199073881506024
1103	20-21	0.25073984706043007
1103	22-23	0.22307815133400766
1103	24-25	0.23766741935352087
1103	26-27	0.21768119490554483
1103	28-29	0.25403248681222124
1103	30-31	0.24615930554318766
1103	32-33	0.22036394570018558
1103	34-35	0.19755314680251956
1103	36-37	0.21635294216080325
1103	38-39	0.27612098246012806
1103	40-41	0.2492438808923083
1103	42-43	0.2664009571688055
1103	44-45	0.2733142939226454
1103	46-47	0.2645086417384661
1103	48-49	0.3230104460016108
1103	50-51	0.36359169146702897
1103	52-53	0.32497345688802426
1103	54-55	0.29509800279362963
1103	56-57	0.3321478828167912
1103	58-59	0.300700292465109
1103	60-61	0.2600754637248741
1103	62-63	0.23045474741178396
1103	64-65	0.30320653175137835
1103	66-67	0.2561507814334405
1103	68-69	0.30131665185865586
1103	70-71	0.2734390438709653
1103	72-73	0.23908282447195006
1103	74-75	0.23841153446936403
1103	76-77	0.27638826339402556
1103	78-79	0.22441161285447464
1103	80-81	0.22843115768732503
1103	82-83	0.2027042703612736
1103	84-85	0.2515844901440474
1103	86-87	0.22853525934547037
1103	88-89	0.24575316746623344
1103	90-91	0.25542398195555904
1103	92-93	0.20484508511253807
1103	94-95	0.2643110062711145
1103	96-97	0.24744674626543883
1103	98-99	0.20153117158533007
1103	100	0.2574842836716087
1104	1	0.14170706388758703
1104	2	0.1229632207145599
1104	3	0.15618770866296927
1104	4	0.1313064443779126
1104	5	0.10544355923983773
1104	6	0.11044468584373845
1104	7	0.1337024673788676
1104	8	0.10513975959272415
1104	9	0.1270628466177257
1104	10-11	0.14274443674814563
1104	12-13	0.1575371064308868
1104	14-15	0.24143560150314158
1104	16-17	0.23353074257813233
1104	18-19	0.21706388307228508
1104	20-21	0.2074789569715847
1104	22-23	0.23969456205549733
1104	24-25	0.2552343035395026
1104	26-27	0.2565807766166941
1104	28-29	0.25963221661999114
1104	30-31	0.2279759045735119
1104	32-33	0.243805507170606
1104	34-35	0.21161198752999866
1104	36-37	0.21619374292554738
1104	38-39	0.2059796821285289
1104	40-41	0.2216626095994627
1104	42-43	0.20807987088125657
1104	44-45	0.19679102481818234
1104	46-47	0.25927169973073205
1104	48-49	0.16855240884054723
1104	50-51	0.15084949606613662
1104	52-53	0.14474545404068806
1104	54-55	0.07308269030621517
1104	56-57	0.16884792406420956
1104	58-59	0.1208921645473211
1104	60-61	0.17213380011208557
1104	62-63	0.15283786325456106
1104	64-65	0.14089389883878312
1104	66-67	0.17494894056420662
1104	68-69	0.11597823097038429
1104	70-71	0.08320182989723435
1104	72-73	0.1036042147257703
1104	74-75	0.04719368408068192
1104	76-77	0.04688949716917534
1104	78-79	0.048221586084970625
1104	80-81	0.07288344042940054
1104	82-83	0.014304408375309663
1104	84-85	0.0552396948996261
1104	86-87	0.07950163457215353
1104	88-89	0.042161136914266706
1104	90-91	0.038865045805394516
1104	92-93	0.0376287252851597
1104	94-95	0.015567475527802799
1104	96-97	-0.005588247388239154
1104	98-99	-0.00967183554429596
1104	100	0.019515101096040155
1105	1	0.12943941458161845
1105	2	0.15887792491592734
1105	3	0.16513458196610742
1105	4	0.13842560270142457
1105	5	0.10501895069209866
1105	6	0.09756611876836274
1105	7	0.10363165244474004
1105	8	0.09660101814106525
1105	9	0.10163557320266392
1105	10-11	0.12676985759428305
1105	12-13	0.1549210564293162
1105	14-15	0.17290218597334217
1105	16-17	0.17144275190007363
1105	18-19	0.19444079357244703
1105	20-21	0.14563324181406756
1105	22-23	0.18920359191143632
1105	24-25	0.16647804464174953
1105	26-27	0.18895082872609237
1105	28-29	0.20733625844346193
1105	30-31	0.20073100645313957
1105	32-33	0.19156591018895597
1105	34-35	0.14642539752363604
1105	36-37	0.17020913559136375
1105	38-39	0.20650026402770294
1105	40-41	0.14948758547132002
1105	42-43	0.15889653765049871
1105	44-45	0.20509865677487227
1105	46-47	0.26037930138119236
1105	48-49	0.22453203885075368
1105	50-51	0.20827602511580068
1105	52-53	0.18667314471105811
1105	54-55	0.14323508422311448
1105	56-57	0.20188168473314505
1105	58-59	0.20123103762735894
1105	60-61	0.17853243659330076
1105	62-63	0.17093425960772635
1105	64-65	0.16748356913219453
1105	66-67	0.09782554241908059
1105	68-69	0.15768600540555155
1105	70-71	0.11732772318609364
1105	72-73	0.06951195568465351
1105	74-75	0.1099930459069185
1105	76-77	0.07202501050650767
1105	78-79	0.04083037163371728
1105	80-81	0.04559189010954867
1105	82-83	0.09068078579307581
1105	84-85	0.10471210563855493
1105	86-87	0.12220251804443194
1105	88-89	0.09578582489874776
1105	90-91	0.1196092728476259
1105	92-93	0.10169078758036676
1105	94-95	0.1337993593457938
1105	96-97	0.07129602078745023
1105	98-99	0.11439170938123056
1105	100	0.14817966132375915
1106	1	0.03548021371356924
1106	2	0.05815302022877944
1106	3	0.08576603913717662
1106	4	0.07865556900976145
1106	5	0.02977560140612212
1106	6	0.02380096149262556
1106	7	0.02650611987419893
1106	8	0.013046892638293173
1106	9	0.06865774094603694
1106	10-11	0.10278696466204451
1106	12-13	0.08716277763659264
1106	14-15	0.08988320579472031
1106	16-17	0.14242848345369907
1106	18-19	0.15521580411562752
1106	20-21	0.12549612449727476
1106	22-23	0.15087274748943003
1106	24-25	0.12104540775890626
1106	26-27	0.14591320995206303
1106	28-29	0.15744396219007228
1106	30-31	0.18788873258948513
1106	32-33	0.1438532322179995
1106	34-35	0.13144451018521863
1106	36-37	0.11663482739191977
1106	38-39	0.176555126390312
1106	40-41	0.195068997354376
1106	42-43	0.15546252237233205
1106	44-45	0.1502242061102379
1106	46-47	0.06861310509550833
1106	48-49	0.0403321943240158
1106	50-51	0.03801132394028883
1106	52-53	0.02540837908298954
1106	54-55	0.0419474719275712
1106	56-57	0.09224415701049082
1106	58-59	0.06356566967201616
1106	60-61	0.038005561743347016
1106	62-63	-0.01607551954694486
1106	64-65	0.07854260792839085
1106	66-67	0.014844346975984024
1106	68-69	-0.02635325718974002
1106	70-71	-0.03544112485456452
1106	72-73	0.01685589194889303
1106	74-75	-0.02199290701388179
1106	76-77	-0.0791660916265684
1106	78-79	-0.08083229831006378
1106	80-81	-0.02754419029290034
1106	82-83	0.02954710265768057
1106	84-85	-0.03209616088977896
1106	86-87	-0.012184650346270587
1106	88-89	0.050233853675358375
1106	90-91	-0.019159506498155565
1106	92-93	-0.015955634594917
1106	94-95	-0.028020881506407136
1106	96-97	-0.06552236596765226
1106	98-99	-0.03654116085461112
1106	100	-0.030262246964596784
1107	1	0.07258699862811113
1107	2	0.08792249329181345
1107	3	0.09162282864254223
1107	4	0.06545999858924034
1107	5	0.04907527819742796
1107	6	0.06583924617277859
1107	7	0.08770415303302315
1107	8	0.0812014563506267
1107	9	0.06610788716928795
1107	10-11	0.09619145650043492
1107	12-13	0.08178894280605675
1107	14-15	0.16656586762640302
1107	16-17	0.1455042620170346
1107	18-19	0.1468407089753896
1107	20-21	0.13689517698060172
1107	22-23	0.11492784501542275
1107	24-25	0.14668695887270644
1107	26-27	0.1436453796694721
1107	28-29	0.18584933620119415
1107	30-31	0.17109248716359104
1107	32-33	0.097491162677521
1107	34-35	0.08700636312695309
1107	36-37	0.08359121544625481
1107	38-39	0.11400808880858904
1107	40-41	0.14881442654646548
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2216	30-31	-0.4041431131853628
2216	32-33	-0.4535052885896249
2216	34-35	-0.4245552543096096
2216	36-37	-0.412844754067919
2216	38-39	-0.4036708941234224
2216	40-41	-0.35385890341944304
2216	42-43	-0.423019711269653
2216	44-45	-0.3737310806048342
2216	46-47	-0.397091545900345
2216	48-49	-0.3696314456515708
2216	50-51	-0.3483061025445906
2216	52-53	-0.35706516650100895
2216	54-55	-0.40318562244520706
2216	56-57	-0.3756467269059769
2216	58-59	-0.3907601730276866
2216	60-61	-0.3898553968504359
2216	62-63	-0.4013982024977025
2216	64-65	-0.3458507027417639
2216	66-67	-0.2835402452331479
2216	68-69	-0.30421867883948295
2216	70-71	-0.32074550408735547
2216	72-73	-0.34697355825267095
2216	74-75	-0.34238241319053486
2216	76-77	-0.3021884021183183
2216	78-79	-0.3481179288482963
2216	80-81	-0.3491118868049483
2216	82-83	-0.32962080711980946
2216	84-85	-0.29599765171431613
2216	86-87	-0.32438256913671637
2216	88-89	-0.30646880708700763
2216	90-91	-0.3374050071997914
2216	92-93	-0.30481821545446053
2216	94-95	-0.3176300843842199
2216	96-97	-0.29206045948377124
2216	98-99	-0.35571988901419616
2216	100	-0.486375405668376
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	35.0
8	120.0
9	204.0
10	334.0
11	305.0
12	256.0
13	277.0
14	432.0
15	827.0
16	1800.0
17	3204.0
18	5688.0
19	9306.0
20	13565.0
21	20646.0
22	30561.0
23	36495.0
24	34953.0
25	28658.0
26	24118.0
27	25038.0
28	29911.0
29	36937.0
30	42877.0
31	54709.0
32	71407.0
33	95937.0
34	146000.0
35	245447.0
36	457250.0
37	1119533.0
38	1358333.0
39	487647.0
40	1027.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.5170317878039	20.317398977271484	13.43957707511324	23.72599215981137
2	15.748259986598159	20.914658578377374	39.279131597182186	24.05794983784228
3	17.653874001041103	26.700311646433907	31.036467331871286	24.609347020653704
4	12.624277488292796	18.020027651562934	38.73547819616551	30.620216663978766
5	12.969311937492012	38.135120579805395	35.01875787282984	13.876809609872756
6	29.34104170720574	38.52338392533496	18.005301997362462	14.130272370096844
7	25.54622228284896	33.55678289206855	23.157425069083303	17.739569755999195
8	26.202923966870134	33.72648949927221	20.68949156207607	19.381094971781582
9	26.663114224769657	14.274908362282465	21.058939436416345	38.00303797653153
10-11	24.78460089433029	25.889454425675762	28.239711814234052	21.086232865759897
12-13	25.868707769605592	24.05708786294387	28.141498809605004	21.932705557845534
14-15	23.22377634022433	24.820402765887508	26.451690608022147	25.50413028586601
16-17	21.839784718371273	27.59957653554339	27.33634834673445	23.22429039935089
18-19	21.568253304922923	26.490651230576674	30.25237696913596	21.68871849536445
20-21	22.935639287847934	25.56727106082135	29.7467470862867	21.75034256504402
22-23	22.988259543739975	25.34928473275338	29.507340180336033	22.15511554317061
24-25	22.63266283273633	25.64955054234428	29.827908826031248	21.889877798888143
26-27	22.411874614920848	25.99462480259882	29.314374912034864	22.27912567044547
28-29	21.86746201244914	26.1218281927649	29.703439053112714	22.30727074167325
30-31	22.484630749632544	25.519701986924918	29.610230495699597	22.38543676774294
32-33	22.654803970098797	25.926109262119134	29.070480431227004	22.348606336555065
34-35	22.617005325648346	26.05226692775	29.504351889479995	21.826375857121658
36-37	22.73596795477901	25.66962946572669	29.202621923192297	22.391780656302
38-39	22.714336175952944	25.584078319114532	29.535927493183156	22.165658011749372
40-41	22.63640829480778	25.244466617097554	29.64438098320889	22.474744104885776
42-43	22.703165955014263	25.448893046610237	29.06584829206274	22.78209270631276
44-45	22.70619675800755	26.044771718889237	28.811139791092987	22.437891732010222
46-47	23.073563700570723	25.792501140270947	28.127709889978536	23.006225269179794
48-49	23.49098763610019	25.87310447462265	27.8920441597505	22.743863729526662
50-51	23.098861332170873	26.15164171232903	27.526097750197486	23.223399205302606
52-53	22.881184363977496	26.39075228897386	27.480586136987707	23.247477210060936
54-55	22.921666161774688	26.391939277919306	27.282279021555844	23.404115538750155
56-57	23.402646153551363	26.175345254353704	27.076230947737773	23.345777644357163
58-59	22.79070404044955	26.31185867294594	27.668955621721903	23.228481664882608
60-61	22.9230980851339	26.58023843952887	27.36640271475149	23.130260760585735
62-63	23.065152453524856	26.296028897336672	27.18376429554758	23.455054353590896
64-65	22.55111015503196	26.393894395405464	27.359517862363152	23.695477587199417
66-67	23.05361358802032	26.136291959986362	27.251126371586455	23.558968080406867
68-69	22.706255742886576	25.931294317747355	27.589203510107023	23.773246429259043
70-71	22.772635321942218	25.81493819928427	27.3929870376352	24.019439441138307
72-73	23.07790312453128	25.803791047006268	27.548651313669588	23.569654514792862
74-75	22.995666314793556	26.22693363399379	27.362782318290613	23.414617732922036
76-77	23.005443825532964	26.1205648584911	26.941723875043856	23.932267440932076
78-79	22.653220204822066	26.11962667135057	27.203228702006587	24.02392442182077
80-81	22.50515672627008	26.296458297097008	27.463654845782948	23.734730130849968
82-83	23.045083170405064	25.75637610117726	27.849305778438993	23.349234949978683
84-85	23.219541426679903	26.00586860524306	27.062546028472102	23.712043939604943
86-87	22.354566934270835	26.45279091061303	27.327230157683747	23.865411997432386
88-89	22.83538954451146	25.78022216410462	27.19946010698632	24.1849281843976
90-91	22.67168114522454	25.826598558934304	27.29845918155297	24.203261114288185
92-93	22.94064631479395	25.847105698909107	27.193859081047457	24.018388905249484
94-95	22.10648018779887	25.890663411680947	27.60689888474463	24.395957515775557
96-97	22.32856519787291	25.82798716371825	27.40774948994303	24.435698148465814
98-99	22.22442983516659	26.12005054952074	27.246111838533515	24.409407776779158
100	22.696800015876626	25.668010172902388	26.94981959727661	24.685370213944378
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	577.0
1	376.5
2	405.0
3	578.5
4	365.5
5	318.0
6	686.0
7	1656.5
8	3158.5
9	3912.0
10	2847.5
11	1707.0
12	1673.5
13	1958.5
14	2415.0
15	3056.0
16	3849.5
17	4589.5
18	5525.0
19	6581.0
20	7549.5
21	8527.5
22	9522.0
23	10772.0
24	12522.5
25	14925.0
26	18330.5
27	22384.5
28	26922.0
29	34423.0
30	44306.5
31	52617.0
32	62426.5
33	70915.0
34	78060.5
35	86124.5
36	96660.0
37	113653.5
38	121163.5
39	118956.0
40	118296.5
41	117884.5
42	119635.5
43	125741.5
44	134520.5
45	153681.0
46	169980.0
47	177998.0
48	185875.0
49	183778.5
50	177632.5
51	179080.0
52	180842.0
53	178395.5
54	173435.0
55	155429.0
56	140984.0
57	125586.5
58	104915.5
59	94409.5
60	80584.0
61	62645.5
62	47747.0
63	37102.0
64	27758.5
65	18944.0
66	13612.0
67	9682.0
68	7081.0
69	5669.5
70	4480.5
71	3549.5
72	2850.5
73	2324.0
74	1746.5
75	1255.0
76	872.5
77	525.5
78	288.5
79	153.5
80	72.5
81	34.0
82	18.0
83	9.5
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	1.0
90	1.5
91	0.5
92	1.5
93	1.5
94	0.0
95	0.5
96	0.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006181796366153045
2	0.08768570196122621
3	5.702764175417939E-4
4	0.0
5	0.03829976420210687
6	0.004744699793947725
7	2.2811056701671752E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.5622113403343503E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	5.246543041384504E-4
24-25	0.0
26-27	0.0
28-29	3.4216585052507626E-5
30-31	1.026497551575229E-4
32-33	0.0015739629124153509
34-35	0.001220391533539439
36-37	7.755759278568397E-4
38-39	6.957372294009885E-4
40-41	0.001380068930451141
42-43	6.273040592959733E-4
44-45	5.132487757876145E-4
46-47	2.8513820877089694E-4
48-49	6.38709587646809E-4
50-51	7.299538144534961E-4
52-53	0.0011747694201360952
54-55	0.0014370965722053206
56-57	9.580643814702137E-4
58-59	0.0024293775387280417
60-61	0.001961750876343771
62-63	0.0010036864948735572
64-65	0.004436750528475157
66-67	0.004185828904756767
68-69	0.0035357137887591216
70-71	0.0016195850258186946
72-73	0.0050526490594202935
74-75	0.0035699303738116295
76-77	0.0035813359021624656
78-79	7.755759278568397E-4
80-81	3.4216585052507626E-5
82-83	1.8248845361337401E-4
84-85	7.983869845585113E-5
86-87	2.2811056701671752E-5
88-89	1.7108292526253816E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	3.421658505250763E-4
98-99	4.334100773317633E-4
100	8.211980412601832E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4383839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.60053492898541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.45862480254699	28.93747236853924
2	16.7243312962968	15.252769068814123
3	7.572586136506468	10.359419358615419
4	4.001403730752958	7.298646023566914
5	2.3986384460980115	5.468959812164983
6	1.4514861403685189	3.9713126665687724
7	1.0288208290095822	3.2840346104243427
8	0.7277272444471296	2.654780130334851
9	0.5262097521813345	2.1595901565885933
>10	2.048098513458995	14.778037353286186
>50	0.04171443860226609	1.2488990595617053
>100	0.01709436190328578	1.4114024335420088
>500	0.0017720528207683752	0.5474431557391578
>1k	0.0011191912552212277	0.8234613410048907
>5k	1.865318758702046E-4	0.5497822929020876
>10k+	1.865318758702046E-4	1.2539901683467203
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15123	0.34497161049938196	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14362	0.3276123963494097	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7471	0.17042140461818966	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	5456	0.12445712536432109	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.2811056701671755E-5
6	0.0	0.0	0.0	0.0	2.2811056701671755E-5
7	0.0	0.0	0.0	0.0	2.2811056701671755E-5
8	0.0	0.0	0.0	0.0	2.2811056701671755E-5
9	0.0	0.0	0.0	0.0	2.2811056701671755E-5
10-11	0.0	0.0	0.0	2.2811056701671755E-5	2.2811056701671755E-5
12-13	0.0	0.0	0.0	3.421658505250763E-5	1.5967739691170229E-4
14-15	0.0	0.0	0.0	4.562211340334351E-5	1.8248845361337404E-4
16-17	0.0	0.0	0.0	4.562211340334351E-5	2.052995103150458E-4
18-19	0.0	0.0	0.0	4.562211340334351E-5	2.2811056701671755E-4
20-21	0.0	0.0	0.0	1.2546081185919465E-4	2.623271520692252E-4
22-23	0.0	0.0	0.0	5.132487757876145E-4	2.7373268042006106E-4
24-25	0.0	0.0	0.0	0.001208986005188603	2.7373268042006106E-4
26-27	0.0	0.0	0.0	0.0023609443686230264	3.421658505250763E-4
28-29	0.0	0.0	0.0	0.006626611971835644	3.7638243557758396E-4
30-31	0.0	0.0	0.0	0.017484674961831398	3.8778796392841984E-4
32-33	0.0	0.0	0.0	0.041333634743429215	4.3341007733176335E-4
34-35	0.0	0.0	0.0	0.08151531112342401	4.562211340334351E-4
36-37	0.0	0.0	0.0	0.14373246827723374	4.562211340334351E-4
38-39	0.0	0.0	0.0	0.22355976120473403	5.018432474367786E-4
40-41	0.0	0.0	0.0	0.3060103256529266	5.018432474367786E-4
42-43	0.0	0.0	0.0	0.3896014429362027	5.360598324892862E-4
44-45	0.0	0.0	0.0	0.49648264911188567	5.58870889190958E-4
46-47	0.0	0.0	0.0	0.6200159266797891	5.816819458926298E-4
48-49	0.0	0.0	0.0	0.7463435586936473	5.930874742434656E-4
50-51	0.0	0.0	0.0	0.8757506833622312	6.387095876468091E-4
52-53	0.0	0.0	0.0	0.9999226705177813	6.843317010501527E-4
54-55	0.0	0.0	0.0	1.1338121678282436	7.071427577518244E-4
56-57	0.0	0.0	0.0	1.2904100720852203	7.41359342804332E-4
58-59	0.0	2.2811056701671755E-5	0.0	1.4659981810463387	7.755759278568397E-4
60-61	0.0	2.2811056701671755E-5	0.0	1.642418893577068	7.983869845585114E-4
62-63	0.0	2.2811056701671755E-5	0.0	1.8154065420741956	8.440090979618549E-4
64-65	0.0	2.2811056701671755E-5	0.0	1.9985907329169708	8.668201546635267E-4
66-67	0.0	2.2811056701671755E-5	0.0	2.2010502666726586	8.668201546635267E-4
68-69	0.0	2.2811056701671755E-5	0.0	2.4208576090499676	8.668201546635267E-4
70-71	0.0	2.2811056701671755E-5	0.0	2.657157345422585	8.782256830143626E-4
72-73	0.0	2.2811056701671755E-5	0.0	2.8933088099266415	9.124422680668702E-4
74-75	0.0	2.2811056701671755E-5	0.0	3.1329731771627563	9.580643814702137E-4
76-77	0.0	2.2811056701671755E-5	0.0	3.3892668047343895	9.808754381718855E-4
78-79	0.0	2.2811056701671755E-5	0.0	3.6616992549224547	9.808754381718855E-4
80-81	0.0	2.2811056701671755E-5	0.0	3.9572963331910684	9.922809665227213E-4
82-83	0.0	2.2811056701671755E-5	0.0	4.259930622452147	0.001026497551575229
84-85	0.0	2.2811056701671755E-5	0.0	4.561618252860107	0.001026497551575229
86-87	0.0	2.2811056701671755E-5	0.0	4.87781371533033	0.0010379030799260649
88	0.0	2.2811056701671755E-5	0.0	5.123842367386211	0.0010493086082769007
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9485	0.0	58.045475	1
GTATCAA	17900	0.0	42.692623	1
CAACGCA	22020	0.0	33.842308	5
ATCAACG	22020	0.0	33.842308	3
TATCAAC	23140	0.0	32.941753	2
AACGCAG	23850	0.0	31.865158	6
GTGGTAT	2300	0.0	31.302818	1
TCAACGC	24470	0.0	30.703709	4
CGCAGAG	25875	0.0	28.770306	8
ACGCAGA	25975	0.0	28.659546	7
GCAGAGT	28945	0.0	25.540232	9
GTACATG	16750	0.0	24.019897	1
GTACTAG	2355	0.0	23.578217	1
GAGTACT	19105	0.0	23.406225	12-13
TACATGG	16840	0.0	23.38721	2
TATTCGC	365	0.0	23.176579	9
ACATGGG	16370	0.0	23.00551	3
CAGAGTA	27965	0.0	22.704409	10-11
AGAGTAC	26820	0.0	22.631083	10-11
AGTACTT	20115	0.0	21.354815	12-13
>>END_MODULE