FastQCFastQC Report
Mon 16 Jan 2017
ERR1146563_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146563_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4331259
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48000.039.5834161
GTATCAA74600.033.727811
CAACGCA74250.032.216885
ATCAACG76500.031.2700483
TATCAAC89550.028.0251452
AACGCAG88800.027.7839166
GTACTAG20550.027.6915131
ACGCAGA92050.025.729357
TCAACGC96200.024.9639324
CGCAGAG95650.024.8597988
GTACATG153000.024.006721
TGATCGC16750.023.8471369
TACATGG151250.023.8326442
GTATAAG26300.023.0678521
ACATGGG152000.022.6328743
GTGATCG18550.021.7864598
TAACGCC10750.021.4217414
TAGGCAT35350.021.1382925
CATGGGG121650.020.7071294
GCAGAGT111700.020.6987449
Produced by FastQC (version 0.11.5)