FastQCFastQC Report
Mon 16 Jan 2017
ERR1146563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4331259
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145000.33477563913864306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138410.31956066353916956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73320.16928103352858834No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT44070.10174870632303448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83500.057.7674371
GTATCAA158350.043.686271
ATCAACG202450.033.4878123
CAACGCA202550.033.4712755
TATCAAC210150.032.8273052
AACGCAG220850.031.112426
TCAACGC222700.030.590564
ACGCAGA235700.028.7923017
CGCAGAG239550.028.329568
GTGGTAT22700.027.7795161
GCAGAGT262850.025.7110429
GTACTAG18900.025.1481081
GTACATG153800.023.590861
TACATGG149100.023.5446282
AGAGTAC249100.022.8003610-11
ACATGGG151800.022.79533
GAGTACT183200.022.3825112-13
CAGAGTA258900.022.01910-11
CATGGGG120300.021.5731094
TAGTACT32800.021.0708854
Produced by FastQC (version 0.11.5)