##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146563_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4331259 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4471937605209 34.0 31.0 34.0 31.0 34.0 2 32.54224741582067 34.0 31.0 34.0 31.0 34.0 3 32.708774515677774 34.0 31.0 34.0 31.0 34.0 4 36.20658058084266 37.0 37.0 37.0 35.0 37.0 5 36.21439516777916 37.0 37.0 37.0 35.0 37.0 6 36.29786420068622 37.0 37.0 37.0 35.0 37.0 7 36.28973769520594 37.0 37.0 37.0 35.0 37.0 8 36.30082292469695 37.0 37.0 37.0 35.0 37.0 9 38.11824737333879 39.0 39.0 39.0 37.0 39.0 10-11 38.08727254592718 39.0 38.0 39.0 37.0 39.0 12-13 38.06176691350021 39.0 38.0 39.0 35.0 39.0 14-15 39.51983511029934 41.0 39.0 41.0 37.0 41.0 16-17 39.51321186287867 41.0 39.0 41.0 37.0 41.0 18-19 39.56653169436416 41.0 39.5 41.0 37.0 41.0 20-21 39.595371461277196 41.0 40.0 41.0 37.0 41.0 22-23 39.55422095515415 41.0 39.5 41.0 37.0 41.0 24-25 39.53692540667737 41.0 39.0 41.0 36.5 41.0 26-27 39.46128365909312 41.0 39.0 41.0 36.0 41.0 28-29 39.376493763129844 41.0 39.0 41.0 36.0 41.0 30-31 39.27218852532255 41.0 39.0 41.0 35.5 41.0 32-33 39.145596926898165 41.0 39.0 41.0 35.0 41.0 34-35 39.059560049399025 41.0 39.0 41.0 35.0 41.0 36-37 38.969084855004056 41.0 39.0 41.0 35.0 41.0 38-39 38.860174951440214 41.0 38.5 41.0 35.0 41.0 40-41 38.76834472378586 40.0 38.0 41.0 35.0 41.0 42-43 38.66202783070696 40.0 38.0 41.0 35.0 41.0 44-45 38.4965724746546 40.0 38.0 41.0 35.0 41.0 46-47 38.239504725993065 40.0 38.0 41.0 34.0 41.0 48-49 38.00238914366469 40.0 38.0 41.0 34.0 41.0 50-51 37.839506942438675 40.0 37.5 41.0 33.0 41.0 52-53 37.70273620210659 40.0 37.0 41.0 33.0 41.0 54-55 37.555242713492774 40.0 37.0 41.0 33.0 41.0 56-57 37.34633925147399 40.0 36.0 41.0 33.0 41.0 58-59 37.12373342254527 39.0 35.5 41.0 33.0 41.0 60-61 36.93705548432915 39.0 35.0 41.0 32.5 41.0 62-63 36.678401476337484 39.0 35.0 41.0 32.0 41.0 64-65 36.385914349615206 38.0 35.0 41.0 32.0 41.0 66-67 36.12951834558959 37.0 35.0 40.0 32.0 41.0 68-69 35.82122599918407 37.0 35.0 40.0 31.0 41.0 70-71 35.55255977534476 36.0 35.0 39.0 31.0 41.0 72-73 35.24088584866433 36.0 35.0 39.0 31.0 41.0 74-75 34.92208766550326 35.0 35.0 38.5 31.0 40.5 76-77 34.628881925555596 35.0 35.0 37.0 31.0 39.5 78-79 34.352609945514686 35.0 35.0 37.0 30.5 39.0 80-81 34.10864231393228 35.0 35.0 36.5 30.0 39.0 82-83 33.86381038400151 35.0 34.5 36.0 30.0 37.5 84-85 33.669293385595275 35.0 34.0 36.0 30.0 37.0 86-87 33.490317480436985 35.0 34.0 35.5 30.0 37.0 88-89 33.34915609987766 35.0 34.0 35.0 30.0 36.0 90-91 33.266641062102266 35.0 34.0 35.0 30.0 36.0 92-93 33.18174842464974 35.0 34.0 35.0 30.0 36.0 94-95 33.10773403299133 35.0 34.0 35.0 30.0 36.0 96-97 33.06391905910037 35.0 34.0 35.0 29.0 35.5 98-99 33.01687176869358 35.0 34.0 35.0 29.0 35.0 100 32.478143191159894 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2518515652144586 1101 2 0.2389891256936849 1101 3 0.25146702906136653 1101 4 0.18019519887118207 1101 5 0.15050062147402343 1101 6 0.15276930661087817 1101 7 0.17369920886156365 1101 8 0.17396321211625576 1101 9 0.20196968394584047 1101 10-11 0.2123559698730304 1101 12-13 0.2340256406308825 1101 14-15 0.30160152701414233 1101 16-17 0.34717434397143876 1101 18-19 0.2855174338615569 1101 20-21 0.2911487916686113 1101 22-23 0.3251633973817434 1101 24-25 0.3125709376637431 1101 26-27 0.31169486602701824 1101 28-29 0.34520753914386404 1101 30-31 0.37864238459362554 1101 32-33 0.3373947823709571 1101 34-35 0.35351657370026857 1101 36-37 0.40951938741741145 1101 38-39 0.4073177209849348 1101 40-41 0.4276049386466383 1101 42-43 0.38290325422056526 1101 44-45 0.43404786282933827 1101 46-47 0.4185151547499686 1101 48-49 0.47210121447164255 1101 50-51 0.4429135456697537 1101 52-53 0.42661139087338995 1101 54-55 0.4088571394480667 1101 56-57 0.3884901365541893 1101 58-59 0.34183731887791424 1101 60-61 0.3106232605717949 1101 62-63 0.3577782399968896 1101 64-65 0.4181399954708951 1101 66-67 0.37274689812904427 1101 68-69 0.4004133059913144 1101 70-71 0.42914159625679105 1101 72-73 0.39916485096069465 1101 74-75 0.363620392726105 1101 76-77 0.29448476751370123 1101 78-79 0.30581569694508914 1101 80-81 0.2880978177574747 1101 82-83 0.2570477198208607 1101 84-85 0.29058245487811263 1101 86-87 0.2983533481392229 1101 88-89 0.318313629983237 1101 90-91 0.2718717021549537 1101 92-93 0.3125372053726423 1101 94-95 0.30053331748464984 1101 96-97 0.24340053743053858 1101 98-99 0.2469403559367649 1101 100 0.2717674714583893 1102 1 0.2589571400035027 1102 2 0.2919875069271072 1102 3 0.2786827284339992 1102 4 0.17600421152739898 1102 5 0.14204086676854644 1102 6 0.17731798781654362 1102 7 0.1901817464783946 1102 8 0.18864363767390557 1102 9 0.23291469001200937 1102 10-11 0.2454697789604836 1102 12-13 0.2526524646997004 1102 14-15 0.33200707241214644 1102 16-17 0.3281542933596242 1102 18-19 0.2934325842264869 1102 20-21 0.344467666956092 1102 22-23 0.34610549895552367 1102 24-25 0.31494168991573446 1102 26-27 0.3321248597325095 1102 28-29 0.37938022366686397 1102 30-31 0.3746929094933691 1102 32-33 0.3673505208297385 1102 34-35 0.3830123620901631 1102 36-37 0.4091713002049602 1102 38-39 0.4412607189037203 1102 40-41 0.4324604211620482 1102 42-43 0.37780803811470065 1102 44-45 0.4413297165588759 1102 46-47 0.4380851937332366 1102 48-49 0.45206194758051765 1102 50-51 0.4417786207976562 1102 52-53 0.42178989505308806 1102 54-55 0.4623596005751196 1102 56-57 0.44599129820692696 1102 58-59 0.4278973994710853 1102 60-61 0.3885092285277736 1102 62-63 0.3859753712890779 1102 64-65 0.43970626005620517 1102 66-67 0.4074279409273771 1102 68-69 0.38315152604415204 1102 70-71 0.3762593081897947 1102 72-73 0.3447696982806363 1102 74-75 0.32938415740973426 1102 76-77 0.38346933832326613 1102 78-79 0.3652021347058394 1102 80-81 0.34749882124469167 1102 82-83 0.38813565267891903 1102 84-85 0.37308945964653617 1102 86-87 0.32056199491209014 1102 88-89 0.36641004336297556 1102 90-91 0.3398816749184874 1102 92-93 0.32916338124258715 1102 94-95 0.3510643704116987 1102 96-97 0.32473002579665433 1102 98-99 0.34289753520067023 1102 100 0.3144160147078807 1103 1 0.15526280992597208 1103 2 0.17542538128358842 1103 3 0.16429450344318042 1103 4 0.16046638841918792 1103 5 0.10455351775641475 1103 6 0.11953113131319526 1103 7 0.11092030272435949 1103 8 0.13484840946296828 1103 9 0.17221051694550482 1103 10-11 0.15920800833764304 1103 12-13 0.1472968405983366 1103 14-15 0.24042200523675916 1103 16-17 0.210041058716385 1103 18-19 0.19501046970493974 1103 20-21 0.2158470961028769 1103 22-23 0.2500324907364728 1103 24-25 0.18586202512106098 1103 26-27 0.24375177739180032 1103 28-29 0.250499229564376 1103 30-31 0.24662461125816293 1103 32-33 0.1715379637392047 1103 34-35 0.1861553240767364 1103 36-37 0.1884040345183493 1103 38-39 0.2257627569394529 1103 40-41 0.19139756656886675 1103 42-43 0.18201185234416783 1103 44-45 0.14775596922970635 1103 46-47 0.15230586747949815 1103 48-49 0.18903633992420055 1103 50-51 0.18360152592540402 1103 52-53 0.17289380526155185 1103 54-55 0.16401856203433596 1103 56-57 0.13826450601668938 1103 58-59 0.11541273306313826 1103 60-61 0.10788209762313983 1103 62-63 0.13875691586441974 1103 64-65 0.18385252963797427 1103 66-67 0.0912557624693946 1103 68-69 0.14132728890739799 1103 70-71 0.09908596821995985 1103 72-73 0.08568954776733051 1103 74-75 0.08671770336887619 1103 76-77 0.08417803526408818 1103 78-79 0.05650200016673068 1103 80-81 0.09312425585135031 1103 82-83 0.07529097211574509 1103 84-85 0.06685231817999693 1103 86-87 0.10118565639792365 1103 88-89 0.03979028323858813 1103 90-91 -0.009658382371526386 1103 92-93 0.01952224429421534 1103 94-95 0.06065233232144607 1103 96-97 0.01344752849413311 1103 98-99 0.03588894368062512 1103 100 0.013774705032020051 1104 1 0.14123147536535896 1104 2 0.16227951345403113 1104 3 0.19330890182749982 1104 4 0.14281380934627919 1104 5 0.10980246099215663 1104 6 0.1025100221436972 1104 7 0.11181195472202177 1104 8 0.11992307479422948 1104 9 0.1874038225627359 1104 10-11 0.16510256741060658 1104 12-13 0.21100113491439743 1104 14-15 0.2622819401036125 1104 16-17 0.2756031093109499 1104 18-19 0.24953193395455742 1104 20-21 0.2268949272276899 1104 22-23 0.26902498182281676 1104 24-25 0.2575569393657773 1104 26-27 0.25195392771119174 1104 28-29 0.2729392336237453 1104 30-31 0.3051479430001294 1104 32-33 0.2532943881694578 1104 34-35 0.2433048107454212 1104 36-37 0.28766891558254315 1104 38-39 0.29537134639627993 1104 40-41 0.2773145563966182 1104 42-43 0.27401786488147906 1104 44-45 0.3200552470196598 1104 46-47 0.2912967973587328 1104 48-49 0.257668774041953 1104 50-51 0.2517581526825907 1104 52-53 0.27723939603698255 1104 54-55 0.2717332331660032 1104 56-57 0.341769398791655 1104 58-59 0.325580647015542 1104 60-61 0.24159868458385603 1104 62-63 0.25542169640657875 1104 64-65 0.25725328886800014 1104 66-67 0.31387539125260133 1104 68-69 0.31872213822972384 1104 70-71 0.3107887732612724 1104 72-73 0.2746550550554119 1104 74-75 0.2849776046062047 1104 76-77 0.3205900926853502 1104 78-79 0.28091495536799016 1104 80-81 0.2634504622303311 1104 82-83 0.25811347878896385 1104 84-85 0.24396633921723065 1104 86-87 0.20916134005315 1104 88-89 0.15733738060751534 1104 90-91 0.2011192098737098 1104 92-93 0.2080237993160594 1104 94-95 0.169415205693916 1104 96-97 0.14524711234022902 1104 98-99 0.14797863142087664 1104 100 0.17569662314275547 1105 1 0.14674636697355936 1105 2 0.15924852171357884 1105 3 0.1394072362234695 1105 4 0.11196740115722292 1105 5 0.06324107539099089 1105 6 0.0737136805143237 1105 7 0.13122703643585965 1105 8 0.10902596744176662 1105 9 0.12741724051150527 1105 10-11 0.13605204305698493 1105 12-13 0.133500662733276 1105 14-15 0.1625728642523896 1105 16-17 0.1605182367825435 1105 18-19 0.15937927936634821 1105 20-21 0.17021109797616418 1105 22-23 0.1990107168687416 1105 24-25 0.18301517140282186 1105 26-27 0.16553899506163106 1105 28-29 0.2132524027498306 1105 30-31 0.24875799646638797 1105 32-33 0.2161760570922766 1105 34-35 0.15383800870213804 1105 36-37 0.13667139645353643 1105 38-39 0.17346532266325454 1105 40-41 0.1130114275827907 1105 42-43 0.12638135045003906 1105 44-45 0.18463875838997268 1105 46-47 0.18375968327350023 1105 48-49 0.19472183811341637 1105 50-51 0.1201979991874893 1105 52-53 0.14077826752301092 1105 54-55 0.12668635904829983 1105 56-57 0.11732537718880565 1105 58-59 0.10509648341868427 1105 60-61 0.10671525220246991 1105 62-63 0.1314723129247568 1105 64-65 0.1202068017981901 1105 66-67 0.06856228919462382 1105 68-69 0.01312981540797864 1105 70-71 0.025487122542024565 1105 72-73 0.021528788739196614 1105 74-75 0.02507973094651561 1105 76-77 0.03504042518090955 1105 78-79 0.0237265521796175 1105 80-81 0.04974342286978839 1105 82-83 0.05144475067940135 1105 84-85 0.04339391290723427 1105 86-87 0.0363945404113295 1105 88-89 0.07613969778904561 1105 90-91 0.09138675105081973 1105 92-93 0.0597121145586712 1105 94-95 0.013930505552181671 1105 96-97 -0.0030317144524829587 1105 98-99 0.034203047371235584 1105 100 0.08750552143656165 1106 1 0.10457851101184445 1106 2 0.11767123490798781 1106 3 0.14354535165214344 1106 4 0.09277898830414699 1106 5 0.014003285659882181 1106 6 0.04821052088989575 1106 7 0.0699429600658874 1106 8 0.08250167931859664 1106 9 0.09057292852403975 1106 10-11 0.10654098453036198 1106 12-13 0.105482393542637 1106 14-15 0.1269585780508251 1106 16-17 0.15607429657293181 1106 18-19 0.19306049765702937 1106 20-21 0.17536819678691984 1106 22-23 0.1429836858563931 1106 24-25 0.11883725320200966 1106 26-27 0.11077951613536641 1106 28-29 0.14458827759620618 1106 30-31 0.14155926035854094 1106 32-33 0.0949066533203009 1106 34-35 0.08418845402835728 1106 36-37 0.14763442565911333 1106 38-39 0.16103174977600077 1106 40-41 0.0985270701972496 1106 42-43 0.06455201720314818 1106 44-45 0.08241693129293992 1106 46-47 0.07771168308367038 1106 48-49 0.06921053234223251 1106 50-51 0.0802564565297601 1106 52-53 0.07970290305974714 1106 54-55 0.05713826044793535 1106 56-57 0.10963335958634701 1106 58-59 0.10244541053196343 1106 60-61 0.10646234029928792 1106 62-63 0.05497390391276724 1106 64-65 0.07271312433094579 1106 66-67 -0.00820770218041389 1106 68-69 0.048551517802224 1106 70-71 -0.014869116188165776 1106 72-73 -0.04267659225153153 1106 74-75 -0.04703111999637599 1106 76-77 -0.06614456904475219 1106 78-79 -0.046271464779195526 1106 80-81 -0.054794547601964894 1106 82-83 -0.01751982322204526 1106 84-85 0.008588203274037198 1106 86-87 0.0250664176458244 1106 88-89 0.015247409764171493 1106 90-91 0.04250049562367053 1106 92-93 -0.06291077838061909 1106 94-95 -0.09278083886287192 1106 96-97 -0.08135241331348908 1106 98-99 0.002287048233093003 1106 100 -0.07189518487355429 1107 1 0.0319066024215644 1107 2 0.0570734712401233 1107 3 0.08236398544859469 1107 4 0.04111262252953196 1107 5 -0.013476301695483528 1107 6 -4.240053843815872E-4 1107 7 0.03898800552619974 1107 8 0.05191532394076148 1107 9 0.049983282539542984 1107 10-11 0.02171630216353293 1107 12-13 0.0736606138237832 1107 14-15 0.1245778689442929 1107 16-17 0.06641353635316705 1107 18-19 0.06326260748646462 1107 20-21 0.05956517992800059 1107 22-23 0.10404853641547618 1107 24-25 0.07917435472810297 1107 26-27 0.06352517528741686 1107 28-29 0.0730974279154637 1107 30-31 0.08840198088360296 1107 32-33 0.06687003155698079 1107 34-35 0.12363924281365968 1107 36-37 0.07719973267783331 1107 38-39 0.10033227179170012 1107 40-41 0.06847109147223307 1107 42-43 0.12898876649349234 1107 44-45 0.19041476999175444 1107 46-47 0.21292082866548867 1107 48-49 0.22974017917179168 1107 50-51 0.22924881387171325 1107 52-53 0.2300652411062103 1107 54-55 0.1608133815409758 1107 56-57 0.18167860606814656 1107 58-59 0.17586695004933262 1107 60-61 0.12727856439855856 1107 62-63 0.11434148004165934 1107 64-65 0.17266222876097004 1107 66-67 0.12077331089110288 1107 68-69 0.18483340115597713 1107 70-71 0.1123337401096407 1107 72-73 0.01711570260731321 1107 74-75 0.026098157312141268 1107 76-77 0.021030131439616184 1107 78-79 0.06349195724686751 1107 80-81 0.11606141985559759 1107 82-83 0.1235204182086207 1107 84-85 0.09046872736396949 1107 86-87 0.09345485263586539 1107 88-89 0.09498435328994503 1107 90-91 0.09953814298074803 1107 92-93 0.10590698474947402 1107 94-95 0.13526840756259162 1107 96-97 0.11785320606959004 1107 98-99 0.10596720126550707 1107 100 0.08513056764465432 1108 1 0.016071229278743715 1108 2 0.047859975580031744 1108 3 0.027071750254641813 1108 4 0.07012267348970624 1108 5 0.008498349391906856 1108 6 0.023570712973402408 1108 7 0.037462690026039525 1108 8 0.05092948339487435 1108 9 0.05202889177226666 1108 10-11 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54-55 -0.323270065312407 2214 56-57 -0.3174374960890418 2214 58-59 -0.28258701046048884 2214 60-61 -0.34303149576295056 2214 62-63 -0.3509929298004053 2214 64-65 -0.2669881952499793 2214 66-67 -0.2901464037189996 2214 68-69 -0.25937727364138397 2214 70-71 -0.32334762185475796 2214 72-73 -0.2387244624792828 2214 74-75 -0.27626817591301744 2214 76-77 -0.2803048347411021 2214 78-79 -0.27732721197379817 2214 80-81 -0.26650332109674224 2214 82-83 -0.22382408069695714 2214 84-85 -0.2675857445039398 2214 86-87 -0.22897898104057646 2214 88-89 -0.20468389525929354 2214 90-91 -0.23286146205825276 2214 92-93 -0.24035965732254994 2214 94-95 -0.25376143250976924 2214 96-97 -0.26750140557346924 2214 98-99 -0.28318058457567474 2214 100 -0.275758863992273 2215 1 -0.2789207476281419 2215 2 -0.3146064426966433 2215 3 -0.30200977487107394 2215 4 -0.261012063498157 2215 5 -0.21068433746551563 2215 6 -0.20642009603893285 2215 7 -0.20092554703144572 2215 8 -0.21958814116089087 2215 9 -0.26445420875568004 2215 10-11 -0.2639008125090001 2215 12-13 -0.27671187860005375 2215 14-15 -0.41411005098616016 2215 16-17 -0.40896594390560637 2215 18-19 -0.3665503778799035 2215 20-21 -0.40451004855739114 2215 22-23 -0.3805942195289873 2215 24-25 -0.4068781188694075 2215 26-27 -0.4195926477738823 2215 28-29 -0.37577198361758235 2215 30-31 -0.3940360771226352 2215 32-33 -0.4856473179644425 2215 34-35 -0.4731208991522493 2215 36-37 -0.46318476633522465 2215 38-39 -0.4419931644076911 2215 40-41 -0.4656127337310352 2215 42-43 -0.5044610627728119 2215 44-45 -0.4745214269931495 2215 46-47 -0.4838485343609449 2215 48-49 -0.4750753275783097 2215 50-51 -0.4794573080479978 2215 52-53 -0.5069772958380696 2215 54-55 -0.5622677974100299 2215 56-57 -0.5295342035217487 2215 58-59 -0.529183837823048 2215 60-61 -0.45212533246003517 2215 62-63 -0.5265129568733187 2215 64-65 -0.4915682209098122 2215 66-67 -0.4694721037924765 2215 68-69 -0.4669258042959754 2215 70-71 -0.44936101397140504 2215 72-73 -0.400661061280708 2215 74-75 -0.4516328854841447 2215 76-77 -0.41096022486792094 2215 78-79 -0.36879164799569253 2215 80-81 -0.337583108261434 2215 82-83 -0.32026578766116387 2215 84-85 -0.3330409427429828 2215 86-87 -0.35256678804054076 2215 88-89 -0.3373499677854639 2215 90-91 -0.36051627088157545 2215 92-93 -0.3580171528172187 2215 94-95 -0.3342866282410597 2215 96-97 -0.30946091992274916 2215 98-99 -0.324374458237223 2215 100 -0.27122384765846164 2216 1 -0.2789249658237978 2216 2 -0.2728727472968018 2216 3 -0.3164162436015019 2216 4 -0.22778686507310653 2216 5 -0.23092584473722866 2216 6 -0.23669674739176116 2216 7 -0.25326123840220305 2216 8 -0.2552123038915184 2216 9 -0.30414890198245104 2216 10-11 -0.33386742931225655 2216 12-13 -0.3456756547741051 2216 14-15 -0.4555102258289523 2216 16-17 -0.41289267356398796 2216 18-19 -0.3894580254403195 2216 20-21 -0.4421130687854813 2216 22-23 -0.4151914780981514 2216 24-25 -0.41643742783269744 2216 26-27 -0.4270266372584004 2216 28-29 -0.4544411768607688 2216 30-31 -0.4066543423061546 2216 32-33 -0.41187150062533107 2216 34-35 -0.44231994589665646 2216 36-37 -0.47021468664813426 2216 38-39 -0.4547036070747623 2216 40-41 -0.4501421756042987 2216 42-43 -0.4663699208704415 2216 44-45 -0.46551256425266985 2216 46-47 -0.46483913656074094 2216 48-49 -0.450223021046277 2216 50-51 -0.3763845365292511 2216 52-53 -0.3902371599045935 2216 54-55 -0.38516291777865064 2216 56-57 -0.32408847046728795 2216 58-59 -0.31847331532166123 2216 60-61 -0.341572114684503 2216 62-63 -0.39049579419591396 2216 64-65 -0.3699426358828788 2216 66-67 -0.3591792251258923 2216 68-69 -0.39633530503146375 2216 70-71 -0.4051505582817114 2216 72-73 -0.39264820229526975 2216 74-75 -0.3747844664865241 2216 76-77 -0.35486997133454423 2216 78-79 -0.3591168765426218 2216 80-81 -0.3530151505815411 2216 82-83 -0.27346407173315157 2216 84-85 -0.356744343005019 2216 86-87 -0.2754175324246759 2216 88-89 -0.2556345295290896 2216 90-91 -0.2963594825308178 2216 92-93 -0.34399404446565285 2216 94-95 -0.32381464611940913 2216 96-97 -0.30984616291912914 2216 98-99 -0.3012242644267076 2216 100 -0.42527572183959705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 26.0 8 132.0 9 196.0 10 300.0 11 311.0 12 304.0 13 272.0 14 408.0 15 801.0 16 1486.0 17 2822.0 18 5246.0 19 8576.0 20 12354.0 21 18784.0 22 27816.0 23 33902.0 24 32583.0 25 26802.0 26 22245.0 27 23227.0 28 28159.0 29 35321.0 30 41286.0 31 52602.0 32 68344.0 33 91973.0 34 137150.0 35 225441.0 36 415630.0 37 1031386.0 38 1430897.0 39 552710.0 40 1766.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.27988653903655 20.33671168701029 13.613837896746828 23.76956387720633 2 15.384597608928344 21.170823712538983 39.75609553194597 23.688483146586705 3 17.38081132386507 26.827732091626373 31.375018989934066 24.41643759457449 4 12.379010352417161 18.249381992626162 39.2446399534177 30.126967701538977 5 12.56138543656357 38.52707287723375 35.47358769034624 13.43795399585644 6 28.806561368056876 39.103514899857956 18.24387538535708 13.84604834672809 7 25.13239802385358 33.874292411119356 23.50601141746809 17.487298147558977 8 25.579791003031684 34.58737978957158 21.005878429343525 18.826950778053217 9 26.321699995313143 14.519958284646567 21.21510165981762 37.94324006022267 10-11 24.199372515012378 26.228574647694813 28.946098582421413 20.625954254871388 12-13 25.365095922455804 24.456041534343708 28.60485831025113 21.574004232949356 14-15 22.62249473738285 25.234911570138696 27.036132116365746 25.10646157611271 16-17 21.387372586123345 27.992045730814063 27.794943687274298 22.82563799578829 18-19 21.196608191752098 27.004769283019094 30.44069865136211 21.357923873866696 20-21 22.447583947300313 26.17078544598695 30.120491524519778 21.261139082192962 22-23 22.47821523545924 25.88358109159552 29.814547249539274 21.823656423405964 24-25 22.10883717644223 26.118918309895577 30.173593867279696 21.598650646382495 26-27 21.939579230888757 26.4021038686442 29.68943902915988 21.968877871307164 28-29 21.608562871175405 26.57849365917404 30.02892347083959 21.78401999881097 30-31 22.114144745576052 26.119865951092695 29.910256957856323 21.855732345474927 32-33 22.21689138160843 26.46207475034003 29.36117340472863 21.959860463322904 34-35 22.295368337108645 26.557919509310217 29.726757586623044 21.419954566958094 36-37 22.337764255310503 26.148808144018275 29.565067920691313 21.948359679979905 38-39 22.24638264217742 26.23599576517965 29.690980712311926 21.826640880331 40-41 22.170304396918777 26.008129149323047 29.810848185729437 22.010718268028736 42-43 22.257689408802364 26.14493076856743 29.32341329554271 22.27396652708749 44-45 22.31757605478251 26.55046659308491 29.200749023979643 21.931208328152945 46-47 22.549402937330612 26.421303063980094 28.459631300049836 22.569662698639455 48-49 22.92947601356059 26.5174534199708 28.30288219612519 22.250188370343427 50-51 22.651691084873494 26.72715143059296 27.963221058413335 22.65793642612021 52-53 22.472808090034057 26.96115434836791 27.919928989746616 22.646108571851418 54-55 22.426190515495527 26.892087565078647 27.87176953092577 22.809952388500047 56-57 22.873676283998606 26.669311029869032 27.64045649435708 22.816556191775284 58-59 22.328420479063897 26.79368436363972 28.17107676820617 22.70681838909021 60-61 22.523271646946302 27.092254828790985 27.830736756070657 22.55373676819206 62-63 22.671570905145767 26.817931993518222 27.618018435685375 22.892478665650632 64-65 22.147483263102494 26.815816721693427 27.84573435529682 23.19096565990726 66-67 22.49065273881362 26.703607401523154 27.771008234317456 23.03473162534577 68-69 22.294286011470714 26.526797420480374 28.01357340537942 23.165343162669487 70-71 22.35768922628654 26.40945098229355 27.83722256469931 23.395637226720602 72-73 22.661447300475324 26.38505978747184 27.914458146922588 23.039034765130253 74-75 22.600499640969634 26.820295490070674 27.673968105173962 22.905236763785727 76-77 22.55114026971193 26.598735541354657 27.420835687910873 23.429288501022548 78-79 22.471059871839998 26.78095915787364 27.425903494079563 23.322077476206797 80-81 22.38984867558671 26.81122052891106 27.599709784052322 23.199221011449907 82-83 22.748454402046523 26.364302242826987 28.111129517385557 22.77611383774093 84-85 22.88786968211292 26.72503696965429 27.319333541894903 23.067759806337886 86-87 22.19333274535943 26.98481595878149 27.568757160159933 23.253094135699143 88-89 22.52762653209129 26.465863342547756 27.58981061600419 23.41669950935676 90-91 22.44763530595499 26.430715972126347 27.69972372922601 23.421924992692656 92-93 22.754146080850855 26.482450022037472 27.50874514777343 23.25465874933824 94-95 22.139523651151276 26.48713993865755 27.86598860354329 23.507347806647882 96-97 22.28134437251226 26.381532771215632 27.765847655636723 23.571275200635384 98-99 22.049287299530445 26.74570489380922 27.555011702775477 23.64999610388486 100 22.44187786177764 26.26666272628707 27.30071222361162 23.99074718832367 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 278.0 1 220.5 2 419.0 3 621.0 4 383.0 5 309.0 6 711.5 7 1822.0 8 3466.5 9 4234.5 10 3128.5 11 1978.0 12 1921.5 13 2190.5 14 2584.5 15 3050.5 16 3624.5 17 4335.5 18 5179.0 19 6018.5 20 7081.5 21 8178.0 22 9604.0 23 11974.0 24 14675.0 25 17771.5 26 22440.0 27 28342.5 28 33654.5 29 40879.5 30 50962.5 31 59757.0 32 70250.5 33 80541.5 34 88566.0 35 97999.5 36 108560.0 37 119445.5 38 124415.5 39 125557.0 40 125804.5 41 126452.0 42 130249.0 43 135723.0 44 144699.5 45 158855.5 46 170075.0 47 177954.0 48 181844.0 49 175776.5 50 168277.5 51 167328.0 52 165242.5 53 160580.0 54 156285.0 55 139855.0 56 121475.0 57 105069.5 58 90604.0 59 83262.0 60 69692.5 61 53054.5 62 41046.0 63 31961.0 64 22815.0 65 14646.5 66 10353.0 67 8035.5 68 6452.5 69 5198.5 70 4056.5 71 3143.0 72 2396.5 73 1899.0 74 1496.5 75 1017.0 76 657.0 77 414.5 78 250.5 79 138.0 80 67.5 81 32.5 82 18.0 83 5.0 84 3.0 85 2.0 86 2.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005864345678704506 2 0.08835768075748876 3 3.924955769211677E-4 4 0.0 5 0.03807207096135327 6 0.004871562748845082 7 2.3087975113009867E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.46319626695148E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.848474773732072E-4 24-25 0.0 26-27 0.0 28-29 3.46319626695148E-5 30-31 9.235190045203947E-5 32-33 0.0015699823076846709 34-35 0.0011543987556504934 36-37 7.157272285033058E-4 38-39 5.656553902687418E-4 40-41 0.0012813826187720475 42-43 5.887433653817516E-4 44-45 5.19479440042722E-4 46-47 3.116876640256332E-4 48-49 7.041832409468009E-4 50-51 7.041832409468009E-4 52-53 0.0012352066685460278 54-55 0.0013275585689980674 56-57 7.734471662858305E-4 58-59 0.002401149411753026 60-61 0.0017662300961452549 62-63 9.119750169638897E-4 64-65 0.004259731408350321 66-67 0.004098115582559251 68-69 0.003290036453603906 70-71 0.001593070282797681 72-73 0.004836930786175567 74-75 0.00344010829183847 76-77 0.003613268105186044 78-79 7.503591911728207E-4 80-81 3.46319626695148E-5 82-83 1.5007183823456414E-4 84-85 9.235190045203947E-5 86-87 2.3087975113009867E-5 88-89 1.385278506780592E-4 90-91 2.3087975113009867E-5 92-93 0.0 94-95 1.1543987556504933E-5 96-97 4.386715271471874E-4 98-99 4.963914649297121E-4 100 8.080791289553454E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4331259.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.23133860919343 #Duplication Level Percentage of deduplicated Percentage of total 1 65.03163937079806 30.715313794331184 2 16.677279605671437 15.75380480271327 3 7.130678074320916 10.103744119242078 4 3.7070626937214017 7.00358133330657 5 2.1516945120414546 5.081370604088659 6 1.352056329246565 3.831565818320858 7 0.9044209558572649 2.990190868794141 8 0.63189352407649 2.3876141600490577 9 0.48530925132899305 2.0629625021724443 >10 1.8565159941507334 14.039316373101798 >50 0.04896866743024838 1.5486464624776497 >100 0.019674122977786136 1.6576872771086617 >500 0.001629811961754755 0.5330740393969584 >1k 9.054510898631249E-4 0.7655482053140732 >5k 9.05451089863125E-5 0.31355787058615636 >10k+ 1.81090217972625E-4 1.212021768996489 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14500 0.33477563913864306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13841 0.31956066353916956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7332 0.16928103352858834 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 4407 0.10174870632303448 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.3087975113009867E-5 2 0.0 0.0 0.0 0.0 2.3087975113009867E-5 3 0.0 0.0 0.0 0.0 2.3087975113009867E-5 4 0.0 0.0 0.0 0.0 2.3087975113009867E-5 5 0.0 0.0 0.0 0.0 2.3087975113009867E-5 6 0.0 0.0 0.0 0.0 4.6175950226019734E-5 7 0.0 0.0 0.0 0.0 4.6175950226019734E-5 8 0.0 0.0 0.0 0.0 4.6175950226019734E-5 9 0.0 0.0 0.0 0.0 4.6175950226019734E-5 10-11 0.0 0.0 0.0 0.0 4.6175950226019734E-5 12-13 0.0 0.0 0.0 0.0 6.92639253390296E-5 14-15 0.0 0.0 0.0 0.0 1.385278506780592E-4 16-17 0.0 0.0 0.0 0.0 2.077917760170888E-4 18-19 0.0 0.0 0.0 0.0 2.3087975113009865E-4 20-21 0.0 0.0 0.0 2.3087975113009867E-5 2.3087975113009865E-4 22-23 0.0 0.0 0.0 2.3087975113009868E-4 2.424237386866036E-4 24-25 0.0 0.0 0.0 7.734471662858305E-4 2.5396772624310853E-4 26-27 0.0 0.0 0.0 0.0014891743947891363 2.5396772624310853E-4 28-29 0.0 0.0 0.0 0.0050793545248621706 2.5396772624310853E-4 30-31 0.0 0.0 0.0 0.014025944881153492 2.5396772624310853E-4 32-33 0.0 0.0 0.0 0.035162986097114025 2.770557013561184E-4 34-35 0.0 0.0 0.0 0.07040678010712359 2.770557013561184E-4 36-37 0.0 0.0 0.0 0.12452499377201873 3.001436764691283E-4 38-39 0.0 0.0 0.0 0.19778313880559903 3.6940760180815787E-4 40-41 0.0 0.0 0.0 0.2728767778606636 3.924955769211677E-4 42-43 0.0 0.0 0.0 0.34854761629355346 3.924955769211677E-4 44-45 0.0 0.0 0.0 0.4448591044774741 3.924955769211677E-4 46-47 0.0 0.0 0.0 0.5555544011567999 4.0403956447767264E-4 48-49 0.0 0.0 0.0 0.6710058207094057 4.617595022601973E-4 50-51 0.0 0.0 0.0 0.7898396286160676 4.617595022601973E-4 52-53 0.0 0.0 0.0 0.9027167389435727 4.963914649297121E-4 54-55 0.0 0.0 0.0 1.026179685860393 5.771993778252466E-4 56-57 0.0 0.0 0.0 1.1715646651470162 6.002873529382565E-4 58-59 0.0 0.0 0.0 1.3282627522390142 6.002873529382565E-4 60-61 0.0 0.0 0.0 1.4866116295515923 6.464633031642762E-4 62-63 0.0 0.0 0.0 1.6450759467397353 6.926392533902959E-4 64-65 0.0 0.0 0.0 1.8113555435036326 7.041832409468009E-4 66-67 0.0 0.0 0.0 1.9956437608556774 7.388152036163157E-4 68-69 0.0 0.0 0.0 2.199060365588851 7.619031787293256E-4 70-71 0.0 0.0 0.0 2.414736685107032 7.619031787293256E-4 72-73 0.0 0.0 0.0 2.6330450337880973 8.196231165118503E-4 74-75 0.0 0.0 0.0 2.8481441539284535 8.311671040683552E-4 76-77 0.0 0.0 0.0 3.0830873886784422 8.427110916248602E-4 78-79 0.0 0.0 0.0 3.3391099447066086 8.542550791813651E-4 80-81 0.0 0.0 0.0 3.611005483624969 9.004310294073848E-4 82-83 0.0 0.0 0.0 3.888926984047825 0.0010158709049724341 84-85 0.0 0.0 0.0 4.1645281429718235 0.0010158709049724341 86-87 0.0 0.0 0.0 4.45771079494438 0.0010274148925289391 88 0.0 0.0 0.0 4.685958516911596 0.0010620468551984538 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8350 0.0 57.767437 1 GTATCAA 15835 0.0 43.68627 1 ATCAACG 20245 0.0 33.487812 3 CAACGCA 20255 0.0 33.471275 5 TATCAAC 21015 0.0 32.827305 2 AACGCAG 22085 0.0 31.11242 6 TCAACGC 22270 0.0 30.59056 4 ACGCAGA 23570 0.0 28.792301 7 CGCAGAG 23955 0.0 28.32956 8 GTGGTAT 2270 0.0 27.779516 1 GCAGAGT 26285 0.0 25.711042 9 GTACTAG 1890 0.0 25.148108 1 GTACATG 15380 0.0 23.59086 1 TACATGG 14910 0.0 23.544628 2 AGAGTAC 24910 0.0 22.80036 10-11 ACATGGG 15180 0.0 22.7953 3 GAGTACT 18320 0.0 22.38251 12-13 CAGAGTA 25890 0.0 22.019 10-11 CATGGGG 12030 0.0 21.573109 4 TAGTACT 3280 0.0 21.070885 4 >>END_MODULE