FastQCFastQC Report
Mon 16 Jan 2017
ERR1146562_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146562_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3448550
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33800.040.0569841
GTATCAA54050.036.878451
CAACGCA58300.031.5125755
ATCAACG59850.030.7754153
TATCAAC69350.028.1857012
AACGCAG70000.027.4521126
GTACATG120800.027.1249121
TACATGG121450.026.269652
GTACTAG15550.025.6975081
TCAACGC74700.025.160264
ACATGGG124900.024.9797253
ACGCAGA73200.024.9030347
CGCAGAG76100.023.9564678
CATGGGG99900.022.9525034
GTGATCG15600.021.385018
TGATCGC15500.021.2198359
GTATAAG23450.021.0498451
GGGCTAT23500.020.5930126
ATGGGGG62900.020.318615
GCAGAGT88200.019.9773739
Produced by FastQC (version 0.11.5)