##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146562_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3448550 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.609028432239636 33.0 31.0 34.0 30.0 34.0 2 31.6828519232721 33.0 31.0 34.0 29.0 34.0 3 31.862346783430716 34.0 31.0 34.0 30.0 34.0 4 35.35164634411564 37.0 35.0 37.0 33.0 37.0 5 35.325550738716274 37.0 35.0 37.0 33.0 37.0 6 35.40362500181236 37.0 35.0 37.0 33.0 37.0 7 35.28335677313654 37.0 35.0 37.0 33.0 37.0 8 35.23310695799684 37.0 35.0 37.0 33.0 37.0 9 36.88016905656001 39.0 38.0 39.0 33.0 39.0 10-11 36.86763204825216 39.0 37.5 39.0 33.0 39.0 12-13 36.84211900653898 39.0 38.0 39.0 33.0 39.0 14-15 38.27220744950776 41.0 39.0 41.0 33.0 41.0 16-17 38.2855500137739 41.0 38.5 41.0 33.0 41.0 18-19 38.31340041466703 40.5 38.5 41.0 33.5 41.0 20-21 38.40560757419785 41.0 39.0 41.0 34.0 41.0 22-23 38.42402488002204 40.5 39.0 41.0 34.0 41.0 24-25 38.476106624523354 41.0 39.0 41.0 34.0 41.0 26-27 38.502096098360184 41.0 39.0 41.0 33.5 41.0 28-29 38.46811819460353 40.0 39.0 41.0 33.0 41.0 30-31 38.458116019776426 40.0 38.0 41.0 33.0 41.0 32-33 38.468489800060894 40.0 38.0 41.0 33.0 41.0 34-35 38.413196850850355 40.0 38.0 41.0 33.0 41.0 36-37 38.32847008162851 40.0 38.0 41.0 33.0 41.0 38-39 38.28518087312059 40.0 38.0 41.0 33.0 41.0 40-41 38.21534021545287 40.0 38.0 41.0 33.0 41.0 42-43 38.09023952095808 40.0 38.0 41.0 33.0 41.0 44-45 37.953446376013105 40.0 38.0 41.0 32.5 41.0 46-47 37.845010656652796 40.0 37.0 41.0 32.0 41.0 48-49 37.71822447115454 40.0 37.0 41.0 32.0 41.0 50-51 37.579510373925274 40.0 37.0 41.0 32.0 41.0 52-53 37.44132809441649 40.0 36.0 41.0 31.5 41.0 54-55 37.24676298734251 40.0 36.0 41.0 31.0 41.0 56-57 37.0934431862667 39.0 35.0 41.0 31.0 41.0 58-59 36.850592277913904 39.0 35.0 41.0 31.0 41.0 60-61 36.65160299256209 39.0 35.0 41.0 31.0 41.0 62-63 36.400083948326106 38.0 35.0 40.0 31.0 41.0 64-65 36.135979179655216 37.0 35.0 40.0 30.0 41.0 66-67 35.86565571037103 37.0 35.0 40.0 30.0 41.0 68-69 35.56751605747343 36.0 35.0 39.5 30.0 41.0 70-71 35.31148482695626 36.0 35.0 39.0 30.0 41.0 72-73 35.003782459294484 35.0 34.0 39.0 30.0 41.0 74-75 34.69318945643821 35.0 34.0 37.5 29.5 40.0 76-77 34.40182525989184 35.0 34.0 37.0 29.0 39.0 78-79 34.138695538704674 35.0 34.0 37.0 29.0 39.0 80-81 33.87956648446449 35.0 34.0 36.0 29.0 39.0 82-83 33.63943237012657 35.0 34.0 36.0 29.0 37.0 84-85 33.42466079946644 35.0 34.0 35.5 29.0 37.0 86-87 33.24119238520538 35.0 34.0 35.0 28.0 37.0 88-89 33.106902611242404 35.0 34.0 35.0 28.0 36.0 90-91 32.9496749358426 35.0 34.0 35.0 28.0 36.0 92-93 32.861775528845456 35.0 34.0 35.0 28.0 36.0 94-95 32.77354670803671 35.0 34.0 35.0 27.5 36.0 96-97 32.69501123660669 35.0 33.0 35.0 27.0 35.0 98-99 32.62473981818445 35.0 33.0 35.0 27.0 35.0 100 31.718814864218295 34.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35228370928339814 1101 2 0.4039131506535263 1101 3 0.4765974146266956 1101 4 0.41077437665909144 1101 5 0.4559406705243134 1101 6 0.4373931833972122 1101 7 0.3908232110932772 1101 8 0.5175847145356585 1101 9 0.5906344152346463 1101 10-11 0.6274125870318912 1101 12-13 0.616274327817969 1101 14-15 0.73261734635458 1101 16-17 0.7487429329283799 1101 18-19 0.6195645504627052 1101 20-21 0.65830429376269 1101 22-23 0.6093264520497428 1101 24-25 0.5780135227598109 1101 26-27 0.6110142510645318 1101 28-29 0.6381538102757176 1101 30-31 0.6367675645727857 1101 32-33 0.6054271481741793 1101 34-35 0.6264165189148159 1101 36-37 0.661295971724158 1101 38-39 0.6207777872951539 1101 40-41 0.6040548270273263 1101 42-43 0.6180311622460621 1101 44-45 0.5311487196286961 1101 46-47 0.47623759373249896 1101 48-49 0.47319276063065985 1101 50-51 0.45033865738968615 1101 52-53 0.476589974484412 1101 54-55 0.49741315480838466 1101 56-57 0.4362367234577036 1101 58-59 0.4497875432899221 1101 60-61 0.47472242744937887 1101 62-63 0.3988511678593696 1101 64-65 0.39364166420713786 1101 66-67 0.40613933739039965 1101 68-69 0.34075635012380445 1101 70-71 0.33953487034217034 1101 72-73 0.352529871289903 1101 74-75 0.3530950539565154 1101 76-77 0.3852260436153543 1101 78-79 0.37379279625954354 1101 80-81 0.40801876651026703 1101 82-83 0.3590973788616765 1101 84-85 0.38372421901247833 1101 86-87 0.4130891822695446 1101 88-89 0.3357466010390553 1101 90-91 0.3526814499104418 1101 92-93 0.2936388217891164 1101 94-95 0.3128888841457709 1101 96-97 0.3818444187222667 1101 98-99 0.3355752998029473 1101 100 0.2795156141321904 1102 1 0.28186371143124944 1102 2 0.2469192412055925 1102 3 0.3585041638709612 1102 4 0.35067661335563116 1102 5 0.3253127933383695 1102 6 0.21522235093954833 1102 7 0.20809395727711433 1102 8 0.2753830413680589 1102 9 0.42414781542549207 1102 10-11 0.40456659378067883 1102 12-13 0.40185722083276687 1102 14-15 0.43321532829720155 1102 16-17 0.44715693837200376 1102 18-19 0.3922573164612615 1102 20-21 0.40721343596241155 1102 22-23 0.446784323547476 1102 24-25 0.4203011614518033 1102 26-27 0.3811823899700215 1102 28-29 0.42963161056541566 1102 30-31 0.37861859294757494 1102 32-33 0.3889957862243776 1102 34-35 0.3889949245600661 1102 36-37 0.3974998667383147 1102 38-39 0.3750245766909188 1102 40-41 0.42454406489387964 1102 42-43 0.4060691597272168 1102 44-45 0.37050376752120684 1102 46-47 0.34528761559641197 1102 48-49 0.330253212430101 1102 50-51 0.3022763758040341 1102 52-53 0.2442019107949065 1102 54-55 0.28607580255680887 1102 56-57 0.2559440244639646 1102 58-59 0.24870698799271196 1102 60-61 0.23350175297200337 1102 62-63 0.24246312780653057 1102 64-65 0.22046820037200376 1102 66-67 0.19647358553119432 1102 68-69 0.2563261299277073 1102 70-71 0.2247284006785435 1102 72-73 0.16783751533610314 1102 74-75 0.1383208277875454 1102 76-77 0.16614251934743152 1102 78-79 0.21212226979393023 1102 80-81 0.2526075373471457 1102 82-83 0.2126157992747082 1102 84-85 0.24471082370313013 1102 86-87 0.25344128636952235 1102 88-89 0.20544642566527926 1102 90-91 0.27883231144119947 1102 92-93 0.2705563334237553 1102 94-95 0.1774560325722092 1102 96-97 0.19428095299106474 1102 98-99 0.14574453832721446 1102 100 0.22438442807001024 1103 1 0.09259795820722516 1103 2 0.12596832725301255 1103 3 0.22563176512350225 1103 4 0.14269779203670652 1103 5 0.13801528714010658 1103 6 0.12736483153971534 1103 7 0.097536818124496 1103 8 0.2685963735444119 1103 9 0.2762174566910147 1103 10-11 0.29521660534207683 1103 12-13 0.2613386481065376 1103 14-15 0.29530669797462394 1103 16-17 0.22964848801606053 1103 18-19 0.21951940113485335 1103 20-21 0.1967482362812234 1103 22-23 0.23928145075247897 1103 24-25 0.2657931680345271 1103 26-27 0.31066769946696837 1103 28-29 0.3404780104554703 1103 30-31 0.352984835060866 1103 32-33 0.30959037829751423 1103 34-35 0.28226731768983626 1103 36-37 0.27939752150433605 1103 38-39 0.28903014823292494 1103 40-41 0.269100375437624 1103 42-43 0.2678374622276394 1103 44-45 0.23442261601569214 1103 46-47 0.2456237489121591 1103 48-49 0.2160810413494545 1103 50-51 0.1719411904730208 1103 52-53 0.21191064173427065 1103 54-55 0.20608156570207115 1103 56-57 0.19435985169599945 1103 58-59 0.22779773019453842 1103 60-61 0.14452465859034191 1103 62-63 0.18494257434394967 1103 64-65 0.2002979693997844 1103 66-67 0.19754769235653669 1103 68-69 0.16634241620505463 1103 70-71 0.15881883440914635 1103 72-73 0.17081127822857667 1103 74-75 0.23684688708524249 1103 76-77 0.16114119768619162 1103 78-79 0.15111589121096358 1103 80-81 0.19325684669763632 1103 82-83 0.1416723588525386 1103 84-85 0.2090791373386054 1103 86-87 0.150229975557437 1103 88-89 0.12991882702094415 1103 90-91 0.226113400951526 1103 92-93 0.20574941383743806 1103 94-95 0.22555289052948524 1103 96-97 0.14606045575551718 1103 98-99 0.09688748690080473 1103 100 0.10978024680530041 1104 1 0.13850862251713636 1104 2 0.1565236448285603 1104 3 0.24900747643322063 1104 4 0.1996932205660542 1104 5 0.19555537675085333 1104 6 0.12097638354010343 1104 7 0.15385297030343992 1104 8 0.21119954163444987 1104 9 0.14297633434724588 1104 10-11 0.260066754968868 1104 12-13 0.25236315390974795 1104 14-15 0.3548642787400311 1104 16-17 0.32021375190332435 1104 18-19 0.31978649924674585 1104 20-21 0.3441493255282282 1104 22-23 0.23825027712527458 1104 24-25 0.2122407622069744 1104 26-27 0.28140235877479114 1104 28-29 0.29055312446042336 1104 30-31 0.24512573638383373 1104 32-33 0.22593379922753343 1104 34-35 0.24802083628684812 1104 36-37 0.24643213970425393 1104 38-39 0.22939675831044326 1104 40-41 0.2542223351804296 1104 42-43 0.2401054956009574 1104 44-45 0.1855072036289087 1104 46-47 0.1924073176202441 1104 48-49 0.20146053616549864 1104 50-51 0.17519318725052102 1104 52-53 0.18922331890411925 1104 54-55 0.2433108603143026 1104 56-57 0.18861033304187202 1104 58-59 0.22396199351364743 1104 60-61 0.24878611018820607 1104 62-63 0.22167535185619158 1104 64-65 0.15262133688199242 1104 66-67 0.15862828566322662 1104 68-69 0.06867570369592357 1104 70-71 0.13479315586552332 1104 72-73 0.14569233206415788 1104 74-75 0.12919417343680806 1104 76-77 0.17580519684749163 1104 78-79 0.1272984764854641 1104 80-81 0.18425223126330081 1104 82-83 0.16682056716370397 1104 84-85 0.16652356838102378 1104 86-87 0.12152404444437792 1104 88-89 0.04724532456649655 1104 90-91 0.1104875742841358 1104 92-93 0.03660734804071808 1104 94-95 0.03733566263081656 1104 96-97 -0.015843620391066793 1104 98-99 0.01138293046046357 1104 100 0.028189772568765648 1105 1 0.07565631619968372 1105 2 0.15235568046814052 1105 3 0.17548111290781065 1105 4 0.13928158684677783 1105 5 0.09444201688845055 1105 6 0.13034406647634 1105 7 0.08025225243328293 1105 8 0.08596832417324407 1105 9 0.09183159309335309 1105 10-11 0.1624614427367419 1105 12-13 0.14932111862790265 1105 14-15 0.1751780391689337 1105 16-17 0.15313397144504393 1105 18-19 0.17649596613311047 1105 20-21 0.17544190391244285 1105 22-23 0.21198315808958057 1105 24-25 0.16981821084986137 1105 26-27 0.15848524492987792 1105 28-29 0.23054978202218734 1105 30-31 0.22555701876480327 1105 32-33 0.19231015952623665 1105 34-35 0.2007848275298656 1105 36-37 0.21422384109298775 1105 38-39 0.20120653166297586 1105 40-41 0.21744942071457274 1105 42-43 0.18553944614549778 1105 44-45 0.0862318779077782 1105 46-47 0.13118208252723917 1105 48-49 0.1026975583511529 1105 50-51 0.1055155378859709 1105 52-53 0.05169697922822536 1105 54-55 0.12733822744799994 1105 56-57 0.018959650279597895 1105 58-59 0.06453751017112097 1105 60-61 0.01952496615542998 1105 62-63 0.01623929518976297 1105 64-65 0.05424330501344343 1105 66-67 0.047809491760851586 1105 68-69 0.07963854160485795 1105 70-71 0.06432185278458746 1105 72-73 0.07134732544943034 1105 74-75 0.03210390533327967 1105 76-77 0.06628828351379923 1105 78-79 0.0834615046994287 1105 80-81 0.09016998220570827 1105 82-83 0.07579289629952513 1105 84-85 0.10769707196476475 1105 86-87 0.10990709873848914 1105 88-89 0.027571235091564006 1105 90-91 0.015897514863787876 1105 92-93 0.056173106391909755 1105 94-95 0.012925648573634874 1105 96-97 -0.02948455186161425 1105 98-99 -0.005983763071974124 1105 100 -0.07826491231611854 1106 1 0.18773546972542832 1106 2 0.13111757969438287 1106 3 0.18505874247624376 1106 4 0.22383320002824547 1106 5 0.1982407713700809 1106 6 0.14220989700567088 1106 7 0.12704695702550595 1106 8 0.2039264468398656 1106 9 0.20066078224877515 1106 10-11 0.22543669557300205 1106 12-13 0.2269705858995934 1106 14-15 0.2251635907918299 1106 16-17 0.22482865955143438 1106 18-19 0.21927913105373165 1106 20-21 0.20373774213860685 1106 22-23 0.2698682176816334 1106 24-25 0.29171483493536243 1106 26-27 0.26614271931219236 1106 28-29 0.2374570164083778 1106 30-31 0.2862453324168328 1106 32-33 0.24000078760763444 1106 34-35 0.23343733237374664 1106 36-37 0.24798503393114402 1106 38-39 0.22743344916532493 1106 40-41 0.2361721386725364 1106 42-43 0.21868259594962325 1106 44-45 0.19551034229660758 1106 46-47 0.19138686681203154 1106 48-49 0.1939831081330965 1106 50-51 0.17877067140366165 1106 52-53 0.19859539772561874 1106 54-55 0.24992558117757113 1106 56-57 0.17799052719576736 1106 58-59 0.19102406407908745 1106 60-61 0.19200040443148225 1106 62-63 0.1689035159402863 1106 64-65 0.1428370094097886 1106 66-67 0.12889252225054548 1106 68-69 0.11486132689204709 1106 70-71 0.14989914560344175 1106 72-73 0.15493080428353778 1106 74-75 0.1773466453082122 1106 76-77 0.18578430509059274 1106 78-79 0.16167899900540306 1106 80-81 0.1700668392027609 1106 82-83 0.17327097706688477 1106 84-85 0.16088989439989376 1106 86-87 0.19379549314422206 1106 88-89 0.22192480518521762 1106 90-91 0.23323376779664784 1106 92-93 0.20622474615342412 1106 94-95 0.1781127131701865 1106 96-97 0.22327759103232125 1106 98-99 0.23616096353929805 1106 100 0.21584120475648305 1107 1 0.05026846919966488 1107 2 0.03765034213714458 1107 3 0.09632354401806253 1107 4 0.10932536247911884 1107 5 -0.00711840687838361 1107 6 0.04941867639697506 1107 7 -0.011659566987447079 1107 8 -0.009860234377462973 1107 9 0.04818937118767508 1107 10-11 0.06000594654558 1107 12-13 0.04269269274352183 1107 14-15 0.06339949199266215 1107 16-17 0.06132951819397903 1107 18-19 0.025271513434816484 1107 20-21 0.03130760477966277 1107 22-23 0.11403571223507925 1107 24-25 0.11013192204125488 1107 26-27 0.15647957046835614 1107 28-29 0.106062694664395 1107 30-31 0.07575118144687565 1107 32-33 0.06260358807883648 1107 34-35 0.06034668851513203 1107 36-37 0.1299666449020478 1107 38-39 0.036844871378036714 1107 40-41 0.012663866035431681 1107 42-43 -0.02109711192897379 1107 44-45 -0.019859717128291265 1107 46-47 -0.008527348474594021 1107 48-49 0.011099067430500043 1107 50-51 0.012624135045982143 1107 52-53 -0.026337231247737236 1107 54-55 -0.0021661022788208584 1107 56-57 0.001058999066579247 1107 58-59 0.050961270623382404 1107 60-61 0.004838124570490265 1107 62-63 -0.006074530809001999 1107 64-65 0.03384665611358173 1107 66-67 0.03830094179943444 1107 68-69 0.07570213639430534 1107 70-71 0.03597213459420345 1107 72-73 0.026814814760648176 1107 74-75 -0.02860183057145349 1107 76-77 0.043714280584083554 1107 78-79 0.08137876046265546 1107 80-81 0.04621230573718549 1107 82-83 0.09066497110291749 1107 84-85 0.10157799756744623 1107 86-87 0.05912502655014862 1107 88-89 0.07449397184459627 1107 90-91 0.06035720790284671 1107 92-93 0.06578477039744968 1107 94-95 0.1132861756671204 1107 96-97 0.08708268593638024 1107 98-99 0.09377269643822927 1107 100 0.003130869150140825 1108 1 0.09135434984916557 1108 2 0.14270969459093052 1108 3 0.12797378898563494 1108 4 0.11749736277087663 1108 5 0.058014024593546765 1108 6 0.011854447094293619 1108 7 0.02131056978158341 1108 8 -0.06533456515894898 1108 9 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-0.23423274428826346 2214 84-85 -0.2225391347256931 2214 86-87 -0.1896043081705514 2214 88-89 -0.2810298390021231 2214 90-91 -0.2095459247842797 2214 92-93 -0.2292959081662218 2214 94-95 -0.23498414108684784 2214 96-97 -0.27408799298086706 2214 98-99 -0.2726585683046423 2214 100 -0.31571892148210523 2215 1 -0.3865684789764643 2215 2 -0.3636720959543709 2215 3 -0.43562355215194515 2215 4 -0.3969155426058322 2215 5 -0.39691956818965934 2215 6 -0.3614681044248371 2215 7 -0.3274901977942051 2215 8 -0.3218585852713076 2215 9 -0.40632895895833343 2215 10-11 -0.37194359332857374 2215 12-13 -0.3962351990650248 2215 14-15 -0.5749920067547478 2215 16-17 -0.5639789009186273 2215 18-19 -0.555301945100247 2215 20-21 -0.48984068391796853 2215 22-23 -0.42240129616652666 2215 24-25 -0.4414627525756032 2215 26-27 -0.45980665350422356 2215 28-29 -0.41441298417296935 2215 30-31 -0.45972292869008413 2215 32-33 -0.49103788583737895 2215 34-35 -0.45011078866776444 2215 36-37 -0.453937740049156 2215 38-39 -0.4896251743271165 2215 40-41 -0.44255959100188136 2215 42-43 -0.4201415841913487 2215 44-45 -0.3705288979198329 2215 46-47 -0.260062940631407 2215 48-49 -0.3259659729379152 2215 50-51 -0.3403299091267371 2215 52-53 -0.3394799845613221 2215 54-55 -0.29447529234067105 2215 56-57 -0.24127703232635866 2215 58-59 -0.26764592048847646 2215 60-61 -0.24767140193424098 2215 62-63 -0.24148739068385794 2215 64-65 -0.25759836921423584 2215 66-67 -0.2606978883757378 2215 68-69 -0.19070024235461602 2215 70-71 -0.22111401622490234 2215 72-73 -0.1979941427551637 2215 74-75 -0.19737527239474417 2215 76-77 -0.2731084152787986 2215 78-79 -0.29667508143441523 2215 80-81 -0.1805281634224798 2215 82-83 -0.14251859995290062 2215 84-85 -0.16573820829019326 2215 86-87 -0.12028936734027695 2215 88-89 -0.17235049663398883 2215 90-91 -0.198604091706315 2215 92-93 -0.17467197367420084 2215 94-95 -0.2004769631841512 2215 96-97 -0.21936609165973664 2215 98-99 -0.21751146221020434 2215 100 -0.16183689760381625 2216 1 -0.17913731250233766 2216 2 -0.16447346796419637 2216 3 -0.21397631566371444 2216 4 -0.20494124636966404 2216 5 -0.12601446735303767 2216 6 -0.14644992953800795 2216 7 -0.17626294309342683 2216 8 -0.18905993724954584 2216 9 -0.2459732182588894 2216 10-11 -0.2838425250202974 2216 12-13 -0.28332250309969 2216 14-15 -0.46835680177174766 2216 16-17 -0.39786242805838157 2216 18-19 -0.461862400329359 2216 20-21 -0.44395754012884225 2216 22-23 -0.40762037885787095 2216 24-25 -0.4734083708007617 2216 26-27 -0.37897240716471714 2216 28-29 -0.4405623693631995 2216 30-31 -0.4439317689211961 2216 32-33 -0.45269249709783566 2216 34-35 -0.38825365391154065 2216 36-37 -0.35810629136851446 2216 38-39 -0.4367808621609015 2216 40-41 -0.41709796597656634 2216 42-43 -0.39107865051717994 2216 44-45 -0.3327324099209932 2216 46-47 -0.3079421066488024 2216 48-49 -0.23696701186155167 2216 50-51 -0.35650918087317507 2216 52-53 -0.23422940414634752 2216 54-55 -0.22687832558783327 2216 56-57 -0.22536748353217462 2216 58-59 -0.22151939383496 2216 60-61 -0.33715021856603755 2216 62-63 -0.2826118792320642 2216 64-65 -0.24811478399735165 2216 66-67 -0.2429407560194079 2216 68-69 -0.23971506312700086 2216 70-71 -0.26096443166882466 2216 72-73 -0.22246919633953866 2216 74-75 -0.22067040540019178 2216 76-77 -0.214031782723211 2216 78-79 -0.2558505011946508 2216 80-81 -0.20089108708312153 2216 82-83 -0.2089861774761701 2216 84-85 -0.13894042038708676 2216 86-87 -0.14639947424574729 2216 88-89 -0.19537038759619918 2216 90-91 -0.1462071878168345 2216 92-93 -0.1273685347987339 2216 94-95 -0.17684921447044388 2216 96-97 -0.2291190605405049 2216 98-99 -0.23159553773498232 2216 100 -0.3932423393234714 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 86.0 1 43.0 2 45.0 3 46.0 4 37.0 5 58.0 6 130.0 7 723.0 8 2091.0 9 2494.0 10 2400.0 11 2880.0 12 3685.0 13 4468.0 14 5498.0 15 6228.0 16 6856.0 17 7670.0 18 8667.0 19 9683.0 20 11201.0 21 12667.0 22 13979.0 23 16725.0 24 20790.0 25 26638.0 26 30702.0 27 30067.0 28 31184.0 29 39168.0 30 45075.0 31 49575.0 32 62134.0 33 84181.0 34 128348.0 35 202027.0 36 387887.0 37 864672.0 38 1001526.0 39 325649.0 40 567.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6914605530749 19.785163883554077 13.076881339329471 24.446494224041555 2 15.378491832754534 20.699664320048722 39.5741287183986 24.34771512879815 3 17.832793382428218 26.063083627669652 30.412368592692957 25.69175439720917 4 12.619658904153685 17.413062145634335 39.05245547074898 30.914823479462996 5 13.467316368090929 38.41111228404349 34.99026143889415 13.131309908971426 6 30.306587768274447 38.08795330130842 18.253131826128115 13.352327104289028 7 26.868163782426496 32.86630265803246 22.933761892651574 17.331771666889473 8 25.88476635904289 34.86214348352612 20.8624485553746 18.390641602056395 9 26.728155635433538 15.30338730655407 20.578340435836054 37.390116622176336 10-11 24.73667993640386 26.213455963008098 28.620292910538385 20.429571190049657 12-13 25.242286552286174 24.226422273064653 27.988198394910953 22.54309277973822 14-15 22.917344858571884 25.41258839211931 25.31839433350816 26.351672415800646 16-17 22.54016790726095 28.0999607622847 26.300767962999966 23.05910336745439 18-19 22.46960380988396 27.970918372969354 27.435117191283574 22.12436062586311 20-21 23.78398756495917 27.116378410356347 26.61181386878248 22.487820155902007 22-23 23.936630254755784 26.84708925826155 26.2892894638896 22.926991023093066 24-25 23.3854851232917 27.102469168918297 26.661833545869822 22.85021216192018 26-27 23.21506428571325 27.419209142109462 26.34762351001314 23.018103062164148 28-29 23.052236681863864 27.436832893338188 26.481951354183114 23.028979070614835 30-31 23.34097009049557 27.2224775863594 26.370302488084484 23.066249835060546 32-33 23.400489241472712 27.469444158465517 25.981477265641878 23.148589334419892 34-35 23.615515042049353 27.300722517767134 26.438985189306557 22.644777250876956 36-37 23.47299119766818 27.086152641424615 26.35476152496411 23.0860946359431 38-39 23.456029425135874 27.22513886204111 26.518469815206863 22.800361897616153 40-41 23.412116918833593 26.79733377610883 26.780497672890903 23.01005163216667 42-43 23.264075857261215 26.895499760205134 26.554508121773157 23.285916260760494 44-45 23.150686262580585 27.33796193232817 26.478094258262246 23.033257546829 46-47 23.257203554772772 27.10077696115542 26.377114772472147 23.264904711599655 48-49 23.457428447943286 26.934218808466447 26.426835175736553 23.181517567853714 50-51 23.295065590242565 27.061138274395326 26.271847943879905 23.371948191482204 52-53 23.18949101166779 27.195988426479868 26.22007092044293 23.394449641409416 54-55 23.222160612428745 27.057019742997717 26.25752993590535 23.46328970866819 56-57 23.510324723377828 26.939415660950257 26.207838742682128 23.342420872989788 58-59 23.222150024221786 26.911882018153282 26.651418062199223 23.21454989542571 60-61 23.240226072808067 27.167158380487432 26.31653529077061 23.27608025593389 62-63 23.35654047267958 26.91651071718487 26.251464677823094 23.475484132312456 64-65 22.982365962196656 27.013398921553755 26.351574984618697 23.652660131630892 66-67 23.419269507947725 26.8000538971513 26.198685843570917 23.58199075133006 68-69 23.046465756428887 26.8490072144174 26.471584356496276 23.632942672657432 70-71 23.197820854325474 26.672777866818866 26.344107466228245 23.785293812627412 72-73 23.372757421114958 26.807838211607926 26.484922806480643 23.33448156079647 74-75 23.28844870491527 27.11351715449335 26.394812902069205 23.203221238522175 76-77 23.29263428437989 26.885536463069894 26.325163064525253 23.496666188024964 78-79 23.14734759762079 26.851731386523376 26.382390408903483 23.61853060695235 80-81 23.107442298442706 26.989681863252198 26.543746196115382 23.359129642189718 82-83 23.509356580442457 26.551895088640144 26.87529751950321 23.063450811414192 84-85 23.57729378738797 26.87064751300443 26.293818571046295 23.258240128561308 86-87 23.061258143915964 27.084886722318664 26.516638237321626 23.337216896443746 88-89 23.465131800884752 26.613722189718903 26.37318633646129 23.547959672935054 90-91 23.31919336016873 26.606940170335236 26.577352972657657 23.496513496838375 92-93 23.57911796354008 26.62067901239044 26.50669371720421 23.293509306865268 94-95 22.980130625292805 26.702255266131264 26.75879284012108 23.558821268454846 96-97 23.203160159893464 26.585834249114665 26.622339804500207 23.58866578649167 98-99 23.073499198116203 26.88461546270972 26.48492084280032 23.556964496373755 100 23.488842730813822 26.493449159742234 26.265810760207252 23.751897349236696 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1079.0 1 803.0 2 587.5 3 609.5 4 569.5 5 534.0 6 476.0 7 453.5 8 475.5 9 499.0 10 438.0 11 383.0 12 395.0 13 407.0 14 438.5 15 511.5 16 560.0 17 653.5 18 849.5 19 1076.5 20 1462.5 21 2165.5 22 3305.0 23 4748.5 24 6418.0 25 8543.5 26 11736.5 27 15811.5 28 20226.5 29 26129.0 30 33203.0 31 40225.0 32 49906.0 33 58097.0 34 63257.5 35 70525.5 36 79784.5 37 91723.0 38 97158.5 39 97826.5 40 98914.5 41 96382.5 42 97167.0 43 103817.0 44 111177.0 45 122413.5 46 131192.0 47 136346.5 48 141874.0 49 140760.0 50 136716.0 51 135588.5 52 135474.5 53 134962.5 54 131884.0 55 122455.0 56 113668.0 57 102685.5 58 90884.0 59 82316.0 60 68808.5 61 53850.0 62 42825.5 63 33788.0 64 24764.0 65 16992.0 66 12001.5 67 8834.5 68 6419.0 69 4626.5 70 3600.0 71 2793.5 72 2140.0 73 1853.0 74 1497.5 75 1081.0 76 812.5 77 540.5 78 335.0 79 203.5 80 126.5 81 99.5 82 81.0 83 54.0 84 36.5 85 28.0 86 25.0 87 19.0 88 12.0 89 15.5 90 15.0 91 12.5 92 10.5 93 9.0 94 8.5 95 6.0 96 5.0 97 5.5 98 7.0 99 8.5 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06115613808702208 2 0.009424250772063622 3 0.011512084789259254 4 0.009018283046497804 5 0.011657073262675616 6 0.011657073262675616 7 0.00281277638427745 8 0.005277580432355628 9 0.006814458250569079 10-11 0.006596975540444534 12-13 0.005959026257412536 14-15 0.010134694291803801 16-17 0.009772223108262893 18-19 0.0074234098389178065 20-21 0.006669469777152716 22-23 0.0026242913688361777 24-25 0.0035522175987009034 26-27 0.004509141523248902 28-29 0.0062780008989285355 30-31 0.009134273825230894 32-33 0.010294181612561801 34-35 0.011584579025967434 36-37 0.017398616809963605 38-39 0.01779008568818779 40-41 0.01747111104667179 42-43 0.02353162923547578 44-45 0.02686636412405214 46-47 0.029461657798205046 48-49 0.030737556364269038 50-51 0.03149149642603413 52-53 0.029722637050354498 54-55 0.03556567252903394 56-57 0.03882791318090212 58-59 0.03595714140725812 60-61 0.0363486102854823 62-63 0.03246291919792376 64-65 0.03430427281031158 66-67 0.03605863333864958 68-69 0.03930637514317612 70-71 0.03849443969204448 72-73 0.035014716330051765 74-75 0.03782749271432921 76-77 0.03755201461483812 78-79 0.03681257340041467 80-81 0.041060735671514116 82-83 0.03761001000420467 84-85 0.033854808542720854 86-87 0.0323179307245074 88-89 0.030128604775920317 90-91 0.03227443418248249 92-93 0.03408679010018704 94-95 0.03726203766800539 96-97 0.03275289614475649 98-99 0.030244595554653402 100 0.028330747705557408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3448550.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.870938024115446 #Duplication Level Percentage of deduplicated Percentage of total 1 69.26382574675576 36.62043438369855 2 15.121802726496474 15.9900778953099 3 6.192789660551689 9.822557950182338 4 3.2096610627478954 6.787911645078419 5 1.8980993902227468 5.017714761203908 6 1.133823017815929 3.596777190315692 7 0.7814111185979605 2.8919757175923246 8 0.5014325002070852 2.1208965313380848 9 0.3696633955184703 1.7590005426817004 >10 1.4705374018838675 12.566992534471854 >50 0.042589679096198776 1.5180624949648276 >100 0.014268607284549803 1.2383861821096906 >500 0.0 0.0 >1k 9.569282138879672E-5 0.06921217105271754 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.1599077873309071E-4 0.0 0.0 0.0 6 0.0 1.1599077873309071E-4 0.0 0.0 2.8997694683272678E-5 7 0.0 1.1599077873309071E-4 0.0 0.0 2.8997694683272678E-5 8 0.0 1.1599077873309071E-4 0.0 0.0 2.8997694683272678E-5 9 0.0 1.1599077873309071E-4 0.0 0.0 2.8997694683272678E-5 10-11 0.0 1.1599077873309071E-4 0.0 0.0 5.7995389366545356E-5 12-13 0.0 1.4498847341636338E-4 0.0 0.0 1.0149193139145438E-4 14-15 0.0 1.7398616809963607E-4 0.0 2.8997694683272678E-5 1.1599077873309071E-4 16-17 0.0 1.7398616809963607E-4 1.4498847341636339E-5 5.7995389366545356E-5 1.3048962607472707E-4 18-19 0.0 1.7398616809963607E-4 2.8997694683272678E-5 5.7995389366545356E-5 1.449884734163634E-4 20-21 0.0 1.7398616809963607E-4 2.8997694683272678E-5 5.7995389366545356E-5 1.7398616809963607E-4 22-23 0.0 1.7398616809963607E-4 2.8997694683272678E-5 1.4498847341636338E-4 1.7398616809963607E-4 24-25 0.0 1.7398616809963607E-4 2.8997694683272678E-5 5.799538936654535E-4 1.7398616809963607E-4 26-27 0.0 1.7398616809963607E-4 2.8997694683272678E-5 0.0014643835815052702 2.0298386278290876E-4 28-29 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.004538139217932174 2.0298386278290876E-4 30-31 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.013817401516579432 2.0298386278290876E-4 32-33 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.03397079932145394 2.3198155746618143E-4 34-35 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.06553478998419626 2.3198155746618143E-4 36-37 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.11454089399892708 2.3198155746618143E-4 38-39 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.1822070145423439 2.6097925214945414E-4 40-41 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.251612996766757 2.6097925214945414E-4 42-43 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.3205405170288962 2.754780994910905E-4 44-45 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.40443084774760407 3.189746415159995E-4 46-47 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.5061257630018414 3.4797233619927214E-4 48-49 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.6126052978788187 3.4797233619927214E-4 50-51 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.7174319641588494 4.349654202490902E-4 52-53 2.8997694683272678E-5 2.0298386278290876E-4 2.8997694683272678E-5 0.8201272998796596 4.6396311493236285E-4 54-55 2.8997694683272678E-5 3.189746415159995E-4 2.8997694683272678E-5 0.9314784474634267 4.6396311493236285E-4 56-57 2.8997694683272678E-5 3.4797233619927214E-4 2.8997694683272678E-5 1.059329283321976 4.6396311493236285E-4 58-59 2.8997694683272678E-5 3.7697003088254486E-4 2.8997694683272678E-5 1.202737382378101 4.6396311493236285E-4 60-61 2.8997694683272678E-5 3.7697003088254486E-4 2.8997694683272678E-5 1.3513940641718984 4.6396311493236285E-4 62-63 2.8997694683272678E-5 3.7697003088254486E-4 2.8997694683272678E-5 1.4972959649707849 4.929608096156356E-4 64-65 2.8997694683272678E-5 4.349654202490902E-4 2.8997694683272678E-5 1.6480259819344363 4.929608096156356E-4 66-67 2.8997694683272678E-5 4.929608096156356E-4 2.8997694683272678E-5 1.8155456641197025 5.364573516405445E-4 68-69 2.8997694683272678E-5 4.929608096156356E-4 2.8997694683272678E-5 2.0011454089399896 5.509561989821809E-4 70-71 2.8997694683272678E-5 5.219585042989083E-4 2.8997694683272678E-5 2.1974018065563787 5.509561989821809E-4 72-73 2.8997694683272678E-5 5.509561989821809E-4 2.8997694683272678E-5 2.392759275637587 5.654550463238173E-4 74-75 2.8997694683272678E-5 5.509561989821809E-4 2.8997694683272678E-5 2.594017775586841 6.089515883487262E-4 76-77 2.8997694683272678E-5 5.509561989821809E-4 2.8997694683272678E-5 2.8096156355569732 6.37949283031999E-4 78-79 2.8997694683272678E-5 5.509561989821809E-4 2.8997694683272678E-5 3.04314856968871 6.37949283031999E-4 80-81 2.8997694683272678E-5 5.509561989821809E-4 2.8997694683272678E-5 3.285206826057329 6.37949283031999E-4 82-83 2.8997694683272678E-5 5.509561989821809E-4 2.8997694683272678E-5 3.5368633193661108 6.669469777152717E-4 84-85 2.8997694683272678E-5 5.509561989821809E-4 2.8997694683272678E-5 3.790767134012846 6.669469777152717E-4 86-87 2.8997694683272678E-5 5.9445274100709E-4 2.8997694683272678E-5 4.055936553044033 6.959446723985444E-4 88 2.8997694683272678E-5 6.089515883487262E-4 2.8997694683272678E-5 4.266459816444593 7.24942367081817E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3380 0.0 40.056984 1 GTATCAA 5405 0.0 36.87845 1 CAACGCA 5830 0.0 31.512575 5 ATCAACG 5985 0.0 30.775415 3 TATCAAC 6935 0.0 28.185701 2 AACGCAG 7000 0.0 27.452112 6 GTACATG 12080 0.0 27.124912 1 TACATGG 12145 0.0 26.26965 2 GTACTAG 1555 0.0 25.697508 1 TCAACGC 7470 0.0 25.16026 4 ACATGGG 12490 0.0 24.979725 3 ACGCAGA 7320 0.0 24.903034 7 CGCAGAG 7610 0.0 23.956467 8 CATGGGG 9990 0.0 22.952503 4 GTGATCG 1560 0.0 21.38501 8 TGATCGC 1550 0.0 21.219835 9 GTATAAG 2345 0.0 21.049845 1 GGGCTAT 2350 0.0 20.593012 6 ATGGGGG 6290 0.0 20.31861 5 GCAGAGT 8820 0.0 19.977373 9 >>END_MODULE