FastQCFastQC Report
Mon 16 Jan 2017
ERR1146562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3448550
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115860.33596729060039726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105990.30734656594800713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60130.17436313813051862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66600.060.0552981
GTATCAA121150.042.9574361
CAACGCA157650.032.5938645
ATCAACG158400.032.5285723
TATCAAC164300.031.6446132
AACGCAG170600.030.6335166
TCAACGC172450.029.9328984
GTGGTAT19750.028.5904351
ACGCAGA185300.027.7464037
CGCAGAG189000.027.178358
GCAGAGT205250.024.8892129
GTACATG120400.023.918371
TACATGG117700.023.7856832
ACATGGG120800.022.8444543
GTACTAG15300.022.7586731
GAGTACT141050.022.4903112-13
AGAGTAC193400.022.21030210-11
CAGAGTA202000.022.10227610-11
TGGTATC25450.021.815712
CATGGGG97450.021.2748434
Produced by FastQC (version 0.11.5)