##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146562_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3448550 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44984500732192 34.0 31.0 34.0 31.0 34.0 2 32.54146699337403 34.0 31.0 34.0 31.0 34.0 3 32.71009902712734 34.0 31.0 34.0 31.0 34.0 4 36.20629510953879 37.0 37.0 37.0 35.0 37.0 5 36.211406533180615 37.0 37.0 37.0 35.0 37.0 6 36.29481144249032 37.0 37.0 37.0 35.0 37.0 7 36.286177088921434 37.0 37.0 37.0 35.0 37.0 8 36.29664177697873 37.0 37.0 37.0 35.0 37.0 9 38.114247437328736 39.0 39.0 39.0 37.0 39.0 10-11 38.08016267706717 39.0 38.0 39.0 36.0 39.0 12-13 38.05472937901437 39.0 38.0 39.0 35.0 39.0 14-15 39.51901712313871 41.0 39.0 41.0 37.0 41.0 16-17 39.51113917443563 41.0 39.0 41.0 37.0 41.0 18-19 39.55920792796972 41.0 39.5 41.0 37.0 41.0 20-21 39.58997085731684 41.0 40.0 41.0 37.0 41.0 22-23 39.547319018138055 41.0 39.5 41.0 37.0 41.0 24-25 39.530166736744434 41.0 39.0 41.0 36.5 41.0 26-27 39.457385857824306 41.0 39.0 41.0 36.0 41.0 28-29 39.37224877122269 41.0 39.0 41.0 36.0 41.0 30-31 39.27143582085224 41.0 39.0 41.0 35.5 41.0 32-33 39.14995809833118 41.0 39.0 41.0 35.0 41.0 34-35 39.065824476954084 41.0 39.0 41.0 35.0 41.0 36-37 38.98066462716214 41.0 39.0 41.0 35.0 41.0 38-39 38.87568659291586 41.0 39.0 41.0 35.0 41.0 40-41 38.781118876049355 40.0 38.0 41.0 35.0 41.0 42-43 38.67617679894448 40.0 38.0 41.0 35.0 41.0 44-45 38.51409940409738 40.0 38.0 41.0 35.0 41.0 46-47 38.26084934247727 40.0 38.0 41.0 34.0 41.0 48-49 38.01888648852416 40.0 38.0 41.0 34.0 41.0 50-51 37.85274941642139 40.0 37.0 41.0 33.0 41.0 52-53 37.71162416087921 40.0 37.0 41.0 33.0 41.0 54-55 37.55499659277088 40.0 36.5 41.0 33.0 41.0 56-57 37.33562018819504 40.0 36.0 41.0 33.0 41.0 58-59 37.098691044061994 39.0 35.0 41.0 33.0 41.0 60-61 36.89565875512897 39.0 35.0 41.0 32.5 41.0 62-63 36.621477722521064 38.5 35.0 41.0 32.0 41.0 64-65 36.31437720201244 37.5 35.0 40.5 32.0 41.0 66-67 36.04553971959229 37.0 35.0 40.0 31.5 41.0 68-69 35.73074915544214 36.5 35.0 39.5 31.0 41.0 70-71 35.460530657812704 36.0 35.0 39.0 31.0 41.0 72-73 35.1507919270418 35.5 35.0 39.0 31.0 41.0 74-75 34.836949587507796 35.0 35.0 38.0 31.0 40.5 76-77 34.55433153064331 35.0 35.0 37.0 31.0 39.5 78-79 34.29459584463035 35.0 35.0 37.0 31.0 39.0 80-81 34.06014904815067 35.0 35.0 36.0 30.0 39.0 82-83 33.834472314451 35.0 34.0 36.0 30.0 37.0 84-85 33.64536573342419 35.0 34.0 36.0 30.0 37.0 86-87 33.477088631453796 35.0 34.0 35.0 30.0 37.0 88-89 33.34287425149701 35.0 34.0 35.0 30.0 36.0 90-91 33.26423003871192 35.0 34.0 35.0 30.0 36.0 92-93 33.18449507764133 35.0 34.0 35.0 30.0 36.0 94-95 33.119841237621614 35.0 34.0 35.0 30.0 36.0 96-97 33.07740267648722 35.0 34.0 35.0 30.0 35.5 98-99 33.03160850212409 35.0 34.0 35.0 29.0 35.0 100 32.48603239042496 35.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20166923488518051 1101 2 0.2357739698650292 1101 3 0.2775031152292726 1101 4 0.22676211288366233 1101 5 0.16363690297430367 1101 6 0.19920194834434568 1101 7 0.16861233740168302 1101 8 0.1639556074297417 1101 9 0.20571825848882952 1101 10-11 0.24121879666267176 1101 12-13 0.2502455714584926 1101 14-15 0.3629696450657889 1101 16-17 0.33671367757693815 1101 18-19 0.3345286300195056 1101 20-21 0.3627377631522677 1101 22-23 0.33816178333314895 1101 24-25 0.31520016065154977 1101 26-27 0.2771083050503975 1101 28-29 0.3541693203317493 1101 30-31 0.3703432472066197 1101 32-33 0.36352230383985784 1101 34-35 0.39518328755396936 1101 36-37 0.4420042222252434 1101 38-39 0.43542877213347 1101 40-41 0.4500603773846521 1101 42-43 0.43790479492053436 1101 44-45 0.4671497222734189 1101 46-47 0.4959881939319004 1101 48-49 0.5032799405320674 1101 50-51 0.47728527240548857 1101 52-53 0.5125415115312606 1101 54-55 0.47144312634196694 1101 56-57 0.46286757762446484 1101 58-59 0.4551847595830836 1101 60-61 0.4489961497975301 1101 62-63 0.47483829803741173 1101 64-65 0.5179730294463667 1101 66-67 0.44775594181304257 1101 68-69 0.4531020425851153 1101 70-71 0.4090565690798158 1101 72-73 0.4092510358623471 1101 74-75 0.37098717544332516 1101 76-77 0.42215853431364536 1101 78-79 0.3702805129526965 1101 80-81 0.42643850717167453 1101 82-83 0.4328731600412681 1101 84-85 0.42170238917713476 1101 86-87 0.47251517926412845 1101 88-89 0.44777071967399706 1101 90-91 0.43144261292174235 1101 92-93 0.425112838882967 1101 94-95 0.3742509349774963 1101 96-97 0.3432916148733227 1101 98-99 0.39261357152366827 1101 100 0.26677513363134864 1102 1 0.20435626128476514 1102 2 0.1793658900306525 1102 3 0.16882183048402055 1102 4 0.17702044622127744 1102 5 0.13165772790420505 1102 6 0.10152419933592682 1102 7 0.1722785897415804 1102 8 0.1628725856978832 1102 9 0.16701804899603445 1102 10-11 0.18867202304181063 1102 12-13 0.19775051588921144 1102 14-15 0.2804186168451537 1102 16-17 0.2835807146541214 1102 18-19 0.2587236345644257 1102 20-21 0.21809128802137678 1102 22-23 0.2517745500099764 1102 24-25 0.2542478805097943 1102 26-27 0.25293326321093446 1102 28-29 0.2265636734296237 1102 30-31 0.2541773609781188 1102 32-33 0.28808470437959244 1102 34-35 0.2754420216872049 1102 36-37 0.26152810784639513 1102 38-39 0.24099777603814942 1102 40-41 0.1969409541604037 1102 42-43 0.18625012557435383 1102 44-45 0.2724278410415053 1102 46-47 0.2673266303870321 1102 48-49 0.31832876309213276 1102 50-51 0.3336682254368668 1102 52-53 0.2410212012126678 1102 54-55 0.22362255535206543 1102 56-57 0.2483190559180457 1102 58-59 0.2807711158832049 1102 60-61 0.25296474591834794 1102 62-63 0.1878277247403659 1102 64-65 0.23031278184715376 1102 66-67 0.21359795434150897 1102 68-69 0.15467876231952005 1102 70-71 0.15874096821266193 1102 72-73 0.23576949892414945 1102 74-75 0.1918090993753907 1102 76-77 0.19596304573092738 1102 78-79 0.20173300145680173 1102 80-81 0.18052279449280917 1102 82-83 0.15451841069012318 1102 84-85 0.22540286329780912 1102 86-87 0.2019826760230785 1102 88-89 0.1736908924888496 1102 90-91 0.13156611449846878 1102 92-93 0.18830258632520014 1102 94-95 0.2551386613306832 1102 96-97 0.2192026156343232 1102 98-99 0.18657324737249326 1102 100 0.24912378575050553 1103 1 0.1766118580311229 1103 2 0.1636535697903767 1103 3 0.20301102516346958 1103 4 0.1596133399471782 1103 5 0.11731943378516974 1103 6 0.10797200023706921 1103 7 0.14583483156897614 1103 8 0.1255660761525803 1103 9 0.17200259283229968 1103 10-11 0.18108932391360355 1103 12-13 0.20784946242236657 1103 14-15 0.2650792709144909 1103 16-17 0.2633338172977986 1103 18-19 0.2867273542759392 1103 20-21 0.2746673678085898 1103 22-23 0.30802585725082565 1103 24-25 0.2695568307395888 1103 26-27 0.2581840452983357 1103 28-29 0.33058949874061483 1103 30-31 0.31399800627984575 1103 32-33 0.27474776301972526 1103 34-35 0.2770149239700501 1103 36-37 0.2874817478820475 1103 38-39 0.28879907843875685 1103 40-41 0.2677468114019277 1103 42-43 0.2597578897699293 1103 44-45 0.197026464808431 1103 46-47 0.17592211828066695 1103 48-49 0.18289469894115484 1103 50-51 0.2070835987397146 1103 52-53 0.20041452609606836 1103 54-55 0.16309917248109684 1103 56-57 0.14820381809175132 1103 58-59 0.17744837924690415 1103 60-61 0.1293129532491335 1103 62-63 0.13452098848125615 1103 64-65 0.13886405553235193 1103 66-67 0.1490513794504622 1103 68-69 0.1691935370878781 1103 70-71 0.14391574098473114 1103 72-73 0.19395830638443812 1103 74-75 0.19030764705276226 1103 76-77 0.1580391172420761 1103 78-79 0.13471494510638138 1103 80-81 0.1723500124886712 1103 82-83 0.178683494456358 1103 84-85 0.20389308977588172 1103 86-87 0.17305110998741924 1103 88-89 0.12790493540296666 1103 90-91 0.14503324074545532 1103 92-93 0.10808319055762183 1103 94-95 0.14102535057175913 1103 96-97 0.1352899917379844 1103 98-99 0.13334445652451166 1103 100 0.1973718949222345 1104 1 0.1614056871845193 1104 2 0.12236134587473657 1104 3 0.17340092659465256 1104 4 0.08825550494397305 1104 5 0.1277316852964674 1104 6 0.08588363550540379 1104 7 0.11072617365596216 1104 8 0.11909073413773541 1104 9 0.21325169852848802 1104 10-11 0.1876065159649727 1104 12-13 0.15464519629258433 1104 14-15 0.202259641847661 1104 16-17 0.22566253745135612 1104 18-19 0.21893809208409465 1104 20-21 0.23097541301076063 1104 22-23 0.24542938140905335 1104 24-25 0.22705931637812427 1104 26-27 0.19787856539537074 1104 28-29 0.2244421182241112 1104 30-31 0.24686026132327754 1104 32-33 0.19571995768637152 1104 34-35 0.25118372869918204 1104 36-37 0.2153758728320767 1104 38-39 0.24214812408883546 1104 40-41 0.18407468534866922 1104 42-43 0.19573940511137522 1104 44-45 0.13041449909859182 1104 46-47 0.16873311806723024 1104 48-49 0.2528799842616465 1104 50-51 0.30316282804521677 1104 52-53 0.23905793860910762 1104 54-55 0.2212925172074094 1104 56-57 0.28310508582376315 1104 58-59 0.2395015561296745 1104 60-61 0.22342288235112306 1104 62-63 0.2368907338827313 1104 64-65 0.21666660828124407 1104 66-67 0.1899435621078851 1104 68-69 0.17763437475746002 1104 70-71 0.20212308269296386 1104 72-73 0.22155880471456868 1104 74-75 0.20084418049070507 1104 76-77 0.21395377210092903 1104 78-79 0.23134329761839467 1104 80-81 0.16797586170153522 1104 82-83 0.2003813997563455 1104 84-85 0.18315797016462199 1104 86-87 0.14668713697886204 1104 88-89 0.1498489621298731 1104 90-91 0.14892379111054055 1104 92-93 0.1449856227633859 1104 94-95 0.14129397050316328 1104 96-97 0.09700439468873867 1104 98-99 0.08582526656837075 1104 100 0.18824454756261133 1105 1 0.17074347055524441 1105 2 0.20816862712916873 1105 3 0.16375081990318563 1105 4 0.11020025159908187 1105 5 0.09802817675343789 1105 6 0.10802658734483117 1105 7 0.12326465729029223 1105 8 0.13353182971393096 1105 9 0.15044082196091324 1105 10-11 0.15146103204445183 1105 12-13 0.16894808310001963 1105 14-15 0.22153949108371052 1105 16-17 0.2125461636075272 1105 18-19 0.20120935953106311 1105 20-21 0.17095815105339085 1105 22-23 0.20201814962410936 1105 24-25 0.21343859561095968 1105 26-27 0.1987756838839303 1105 28-29 0.22555975696862163 1105 30-31 0.22610770504194022 1105 32-33 0.18242916240351548 1105 34-35 0.16826701643787345 1105 36-37 0.21731601267435252 1105 38-39 0.23410161176354904 1105 40-41 0.16539655390842967 1105 42-43 0.1925922127945583 1105 44-45 0.1687805299303946 1105 46-47 0.1992902986627172 1105 48-49 0.14069102742504214 1105 50-51 0.1294080353465361 1105 52-53 0.1285486622031371 1105 54-55 0.14628463459467866 1105 56-57 0.17253283181389634 1105 58-59 0.07086912455321226 1105 60-61 0.06341945661498727 1105 62-63 0.09631233687121465 1105 64-65 0.07530128714763862 1105 66-67 0.04598107790818062 1105 68-69 0.0404080829208624 1105 70-71 -0.003852744428975541 1105 72-73 0.04792735868389997 1105 74-75 0.04570888188786171 1105 76-77 0.03360222335604135 1105 78-79 0.06661315391109213 1105 80-81 0.06038374385830991 1105 82-83 0.10042239548027254 1105 84-85 0.046136393901612394 1105 86-87 0.11223211837624802 1105 88-89 0.1056323930006755 1105 90-91 0.05468016331295189 1105 92-93 0.09290776955569413 1105 94-95 0.05701189589769484 1105 96-97 0.04298052694407062 1105 98-99 0.0636066013130332 1105 100 0.07422242293630887 1106 1 0.08042533115043682 1106 2 0.0903499204419802 1106 3 0.08486644560065315 1106 4 0.11827553953754943 1106 5 0.049530073658836216 1106 6 0.06650176540530595 1106 7 0.09173616792448058 1106 8 0.06853076340922115 1106 9 0.12519974504900233 1106 10-11 0.08662672710860164 1106 12-13 0.09753340598970084 1106 14-15 0.13841656073357456 1106 16-17 0.13944877285825186 1106 18-19 0.1441916162620771 1106 20-21 0.14795647950957402 1106 22-23 0.12285088912949504 1106 24-25 0.11112714486358044 1106 26-27 0.15192399249298916 1106 28-29 0.21170855187881443 1106 30-31 0.22473693982817622 1106 32-33 0.17968754923652597 1106 34-35 0.19353181888811832 1106 36-37 0.22408831969647025 1106 38-39 0.3035047065914398 1106 40-41 0.2589783717480074 1106 42-43 0.21101659296746078 1106 44-45 0.22102960547213968 1106 46-47 0.28337534406495735 1106 48-49 0.20058005726978223 1106 50-51 0.18706018337651642 1106 52-53 0.15073501917051857 1106 54-55 0.21144516051147377 1106 56-57 0.1899472321607334 1106 58-59 0.18734463894956832 1106 60-61 0.15405587401318854 1106 62-63 0.17133784647902672 1106 64-65 0.18641377495132616 1106 66-67 0.14859978161675258 1106 68-69 0.09252692656254169 1106 70-71 0.09726744434936307 1106 72-73 0.15753525020750914 1106 74-75 0.08433389282498638 1106 76-77 0.07015281139567975 1106 78-79 0.05290012370891617 1106 80-81 0.1129934186240007 1106 82-83 0.1564834431313713 1106 84-85 0.13108540467904817 1106 86-87 0.07322420993241252 1106 88-89 0.13699021155842672 1106 90-91 0.10260283419216876 1106 92-93 0.1331920788663865 1106 94-95 0.09941849287981341 1106 96-97 0.10431356710393658 1106 98-99 0.06071827040982214 1106 100 0.1179536578867797 1107 1 0.013821435402924465 1107 2 0.06632622365773244 1107 3 0.06488039705055826 1107 4 0.07221654574411929 1107 5 0.08827752139458767 1107 6 0.06834309430875152 1107 7 0.08016298684277956 1107 8 0.09536634549250778 1107 9 0.09510684939137093 1107 10-11 0.07999608975642758 1107 12-13 0.07873472207117516 1107 14-15 0.12224093234165423 1107 16-17 0.12283030126583583 1107 18-19 0.13773044050641658 1107 20-21 0.12157529392267463 1107 22-23 0.13512371076648577 1107 24-25 0.13121524421317332 1107 26-27 0.13427147109056392 1107 28-29 0.18256252935447037 1107 30-31 0.10106544954447827 1107 32-33 0.11013585291043881 1107 34-35 0.12220294328430015 1107 36-37 0.1963551598680624 1107 38-39 0.16120051407734337 1107 40-41 0.1579372626952349 1107 42-43 0.10881311496803647 1107 44-45 0.07409977951527935 1107 46-47 0.1368733479894999 1107 48-49 0.07989679415769046 1107 50-51 0.012106774378707996 1107 52-53 -0.00297728745594128 1107 54-55 0.031722805500365325 1107 56-57 2.3085861504057448E-4 1107 58-59 0.035742847055253435 1107 60-61 -0.015196232027378187 1107 62-63 0.002049092031704447 1107 64-65 0.03861846594739404 1107 66-67 -0.00430934226559998 1107 68-69 0.012016640748711893 1107 70-71 0.023728667829473693 1107 72-73 0.005253026852273024 1107 74-75 -0.02801819118279525 1107 76-77 -0.04666435302382865 1107 78-79 -0.047880906932576295 1107 80-81 0.025939135234580135 1107 82-83 0.009248319839208818 1107 84-85 -0.01022234092095431 1107 86-87 -0.005890334334800684 1107 88-89 -0.024712263034260218 1107 90-91 -0.018712320629951762 1107 92-93 -0.03922596829651326 1107 94-95 0.0019375687434077804 1107 96-97 -0.040437140212297606 1107 98-99 -0.06788214224765454 1107 100 0.057541334477214434 1108 1 0.014317810938131004 1108 2 0.05434228893689408 1108 3 0.0569975159153131 1108 4 0.04703206351260292 1108 5 0.03464157253903721 1108 6 -0.0024061324565778364 1108 7 0.003962475025829804 1108 8 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42-43 -0.25284385781163365 2214 44-45 -0.20328402011800506 2214 46-47 -0.16754932484623453 2214 48-49 -0.14236677686318444 2214 50-51 -0.13435342049241683 2214 52-53 -0.14551272086487899 2214 54-55 -0.19683733027298445 2214 56-57 -0.10913664367058118 2214 58-59 -0.14043759309689108 2214 60-61 -0.1686038248285584 2214 62-63 -0.18043738367001794 2214 64-65 -0.11699765360184955 2214 66-67 -0.09077822501460986 2214 68-69 -0.09441055786527386 2214 70-71 -0.12966718118328657 2214 72-73 -0.10272043723698943 2214 74-75 -0.07905827412172073 2214 76-77 -0.04935323860144791 2214 78-79 -0.10559247191234533 2214 80-81 -0.12699453887907453 2214 82-83 -0.11235867238715969 2214 84-85 -0.04880466361500879 2214 86-87 -0.08775123231790616 2214 88-89 -0.07459709814136772 2214 90-91 -0.09443285887429909 2214 92-93 -0.07920195035504207 2214 94-95 -0.10496304344891172 2214 96-97 -0.09051997811771173 2214 98-99 -0.09233371995618 2214 100 -0.12550442336333845 2215 1 -0.2289294344769246 2215 2 -0.29164174962863854 2215 3 -0.32002665547961584 2215 4 -0.1672802561566158 2215 5 -0.18049249135997059 2215 6 -0.17882930350106108 2215 7 -0.21453039134960505 2215 8 -0.18844269558221782 2215 9 -0.24348885631096806 2215 10-11 -0.23772683518402005 2215 12-13 -0.2505829878469541 2215 14-15 -0.3808671046071481 2215 16-17 -0.3393066707167023 2215 18-19 -0.3536093872991586 2215 20-21 -0.3432012726887521 2215 22-23 -0.3257793876927195 2215 24-25 -0.3278062020497714 2215 26-27 -0.31839516194232687 2215 28-29 -0.3348892626689661 2215 30-31 -0.30001971275689243 2215 32-33 -0.27583834945664165 2215 34-35 -0.3595928403012536 2215 36-37 -0.3134425171940691 2215 38-39 -0.31420045047633494 2215 40-41 -0.35128496145392063 2215 42-43 -0.334839402914362 2215 44-45 -0.3654404178414197 2215 46-47 -0.3266366309946491 2215 48-49 -0.2865047174704003 2215 50-51 -0.22833558337011794 2215 52-53 -0.2851914174263186 2215 54-55 -0.303100924601182 2215 56-57 -0.2257120564559898 2215 58-59 -0.21832394962699198 2215 60-61 -0.26078790798274554 2215 62-63 -0.22868939536272848 2215 64-65 -0.20023686361370352 2215 66-67 -0.18707121669704918 2215 68-69 -0.22155181371088872 2215 70-71 -0.15363932090741628 2215 72-73 -0.23241533253935387 2215 74-75 -0.1741189853078069 2215 76-77 -0.1414999784465465 2215 78-79 -0.12073501937133813 2215 80-81 -0.16958025437541835 2215 82-83 -0.1725262192860697 2215 84-85 -0.11113076823179568 2215 86-87 -0.1447159847148427 2215 88-89 -0.1242151663124531 2215 90-91 -0.1733355329815396 2215 92-93 -0.13184866195218348 2215 94-95 -0.11866061061817135 2215 96-97 -0.11192218710777269 2215 98-99 -0.20806897525437051 2215 100 -0.1274202678359444 2216 1 -0.2354824276235945 2216 2 -0.302084593571891 2216 3 -0.3018001063403375 2216 4 -0.2344405587647529 2216 5 -0.2700114231878601 2216 6 -0.2857656269210551 2216 7 -0.2801956310221243 2216 8 -0.30294419986248755 2216 9 -0.32950556300650646 2216 10-11 -0.35302893563468984 2216 12-13 -0.3488748766900187 2216 14-15 -0.458732362741479 2216 16-17 -0.38877306170326165 2216 18-19 -0.39990511706763243 2216 20-21 -0.403904917430836 2216 22-23 -0.3762034992983274 2216 24-25 -0.39358435689505455 2216 26-27 -0.4035482666153527 2216 28-29 -0.35737727385752294 2216 30-31 -0.37763739216054404 2216 32-33 -0.42181837364971386 2216 34-35 -0.46063086143208665 2216 36-37 -0.4581398616676111 2216 38-39 -0.38445126307765776 2216 40-41 -0.3789470299562936 2216 42-43 -0.43880948636505224 2216 44-45 -0.49595900271301474 2216 46-47 -0.47577791811768577 2216 48-49 -0.463351287125775 2216 50-51 -0.41240066032898426 2216 52-53 -0.40172306503625066 2216 54-55 -0.4224727891665836 2216 56-57 -0.42176423710877486 2216 58-59 -0.42015922154480023 2216 60-61 -0.46438197169373296 2216 62-63 -0.4901392189429501 2216 64-65 -0.4955324334416389 2216 66-67 -0.45865656811577793 2216 68-69 -0.42596330229590507 2216 70-71 -0.3754423133811642 2216 72-73 -0.3437134003440576 2216 74-75 -0.3784502887390886 2216 76-77 -0.35696026871137576 2216 78-79 -0.39295956747824334 2216 80-81 -0.3601296185840539 2216 82-83 -0.33926218748922565 2216 84-85 -0.3657411940344062 2216 86-87 -0.32769677517941176 2216 88-89 -0.3025723156920037 2216 90-91 -0.30885125889614784 2216 92-93 -0.35000591030895123 2216 94-95 -0.37474327655806405 2216 96-97 -0.35781639622191364 2216 98-99 -0.3603056355040408 2216 100 -0.5047986991695979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 20.0 8 81.0 9 105.0 10 233.0 11 226.0 12 185.0 13 197.0 14 328.0 15 588.0 16 1130.0 17 2141.0 18 3916.0 19 6328.0 20 9313.0 21 14044.0 22 20469.0 23 25643.0 24 25348.0 25 21523.0 26 18433.0 27 19195.0 28 22822.0 29 28047.0 30 33197.0 31 42299.0 32 55198.0 33 74457.0 34 110926.0 35 182563.0 36 344603.0 37 861108.0 38 1117670.0 39 405093.0 40 1120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.540586030320554 19.802201282118947 13.497512146374763 24.159700541185735 2 15.597428127195844 20.821425036722587 39.24805256358646 24.333094272495106 3 17.53413114024875 26.378925323985214 31.006307892303315 25.080635643462724 4 12.586797349610707 17.721999101071464 38.55777065723275 31.13343289208508 5 12.913266684052763 38.10864995029298 35.17232396505104 13.805759400603215 6 29.784012061291264 38.36431351817531 17.845502839443483 14.00617158108994 7 25.88877985749365 33.61179835687367 22.906423225079074 17.592998560553603 8 26.0972582679677 34.1119890968668 20.72821910658103 19.062533528584478 9 26.843861912977918 14.36966261182236 20.667787910861087 38.118687564338636 10-11 24.65375302663438 25.86362384190457 28.52191500775688 20.960708123704165 12-13 25.74218149657102 23.979382639080193 28.300039146887823 21.978396717460964 14-15 23.03692408559717 24.810032394788706 26.478024015031252 25.67501950458287 16-17 21.802714184222356 27.584898000608955 27.35690362616172 23.255484189006975 18-19 21.757796755157965 26.438561134389815 29.96527526061678 21.838366849835438 20-21 22.880413507126182 25.723854953531195 29.507314668483854 21.88841687085877 22-23 22.977672386732618 25.48800105552144 29.23958234408404 22.2947442136619 24-25 22.473996317292773 25.720491220947935 29.585883922228184 22.219628539531108 26-27 22.302851923272097 26.05567557379188 29.286279740760605 22.355192762175406 28-29 22.189498304793787 26.01919706496763 29.49806701255137 22.29323761768721 30-31 22.495411471300002 25.750986901579232 29.30781977503269 22.445781852088075 32-33 22.673449526982196 26.00764217212373 28.928596698386777 22.390311602507296 34-35 22.76115302138173 26.023421743625136 29.221474233037213 21.99395100195592 36-37 22.695768430481543 25.79286560508067 29.044550857259182 22.466815107178604 38-39 22.773362926666174 25.826596273275715 29.21513244287521 22.184908357182906 40-41 22.59824422110728 25.63788640735764 29.315038025250413 22.448831346284674 42-43 22.758606290691322 25.654120429493894 28.900407826652796 22.686865453161992 44-45 22.6597821883843 26.17021946014397 28.616725297920375 22.553273053551358 46-47 22.95741429089039 26.049447889461614 28.111755715716757 22.881382103931244 48-49 23.290433380624048 26.101937711095534 27.839606904686132 22.768022003594286 50-51 23.077379236670087 26.34915769712358 27.466346452190244 23.10711661401609 52-53 22.87176948732414 26.540519230872395 27.415246232098582 23.17246504970488 54-55 22.890625747607874 26.501341598185178 27.31743578873011 23.290596865476836 56-57 23.258043427104628 26.362916554359856 27.22743372544903 23.151606293086488 58-59 22.825556160010766 26.361677127350465 27.713813786295827 23.098952926342946 60-61 22.95478673351353 26.663595747574508 27.364310468470304 23.01730705044166 62-63 23.014947610722423 26.425738528889713 27.332622688458873 23.226691171928987 64-65 22.65760932606426 26.458908479294745 27.449324324324326 23.434157870316668 66-67 22.97207988609243 26.27203551781836 27.315327933743376 23.440556662345834 68-69 22.71323737093417 26.190061923760176 27.552665934740233 23.54403477056542 70-71 22.844471483630677 26.004373504600416 27.443973352997965 23.70718165877095 72-73 23.03917623891925 26.011544266384913 27.52248629319548 23.426793201500356 74-75 22.993053212499547 26.471333246433847 27.212554936255746 23.32305860481086 76-77 22.975818964336042 26.27555677221387 27.07812594472463 23.670498318725457 78-79 22.762329927041165 26.27909943975967 27.205235869300452 23.753334763898714 80-81 22.727753825668078 26.384336248183292 27.29877908035498 23.589130845793647 82-83 23.086395301901323 25.987866183525572 27.634503141035754 23.291235373537354 84-85 23.259738086794236 26.224566462339066 26.994267440751134 23.521428010115564 86-87 22.676084539311383 26.541303814835775 27.197115772877844 23.585495872975002 88-89 22.931573015804204 26.11839574637938 27.11175685858193 23.838274379234488 90-91 22.792275014136376 26.0074524075336 27.31242116251758 23.88785141581244 92-93 23.032825390381465 26.05458816024126 27.14762726363254 23.764959185744733 94-95 22.446915725002643 26.082879193121627 27.48107573923471 23.989129342641014 96-97 22.638486000357545 25.96784228198964 27.331580994767485 24.06209072288533 98-99 22.53365783893882 26.21931734351584 27.15957359197895 24.087451225566387 100 22.817220221851244 25.92538785400259 26.966876002420744 24.29051592172542 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 367.0 1 252.0 2 334.0 3 518.0 4 324.5 5 241.0 6 591.0 7 1564.0 8 2918.0 9 3573.5 10 2623.5 11 1579.5 12 1546.5 13 1735.5 14 2085.0 15 2587.5 16 3083.5 17 3596.0 18 4304.0 19 4999.5 20 5783.0 21 6721.0 22 7938.0 23 9396.5 24 10847.5 25 12986.0 26 15888.0 27 19171.5 28 23035.5 29 28618.5 30 35052.0 31 40966.5 32 49659.0 33 57005.0 34 61663.5 35 68558.0 36 77246.0 37 89142.0 38 94685.5 39 95174.5 40 96182.0 41 94031.5 42 94846.5 43 101128.0 44 108887.5 45 120503.0 46 128536.5 47 133936.5 48 140194.5 49 138313.0 50 134261.5 51 132905.0 52 131807.5 53 131345.0 54 129763.5 55 120807.0 56 111765.0 57 101596.0 58 90492.0 59 81879.0 60 67801.5 61 52661.0 62 41528.0 63 32554.5 64 23739.0 65 15989.0 66 11132.0 67 8233.5 68 5965.5 69 4152.5 70 3191.5 71 2589.5 72 2040.0 73 1751.0 74 1363.5 75 916.0 76 632.5 77 401.5 78 252.0 79 131.0 80 62.5 81 42.5 82 25.5 83 18.0 84 11.5 85 3.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005683548157921445 2 0.09055980049586057 3 3.7697003088254486E-4 4 0.0 5 0.03917588551710139 6 0.004726624233373447 7 5.799538936654536E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.349654202490902E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.074596569572719E-4 24-25 0.0 26-27 0.0 28-29 5.799538936654536E-5 30-31 1.3048962607472707E-4 32-33 0.0015658755128967249 34-35 0.0013048962607472706 36-37 8.844296878398167E-4 38-39 7.394412144234534E-4 40-41 0.0015078801235301794 42-43 7.539400617650897E-4 44-45 5.654550463238173E-4 46-47 3.044757941743631E-4 48-49 6.524481303736353E-4 50-51 8.119354511316352E-4 52-53 0.001319395108088907 54-55 0.0014788824288469067 56-57 0.0010294181612561801 58-59 0.00237781096402836 60-61 0.0020588363225123602 62-63 0.0011309100926476345 64-65 0.004349654202490901 66-67 0.0040596772556581754 68-69 0.0033637325832596306 70-71 0.0016528685969465426 72-73 0.004973104638181265 74-75 0.0035232199040176305 76-77 0.003610212988067449 78-79 8.699308404981804E-4 80-81 5.799538936654536E-5 82-83 1.7398616809963607E-4 84-85 8.699308404981803E-5 86-87 1.449884734163634E-5 88-89 1.5948732075799974E-4 90-91 0.0 92-93 0.0 94-95 1.449884734163634E-5 96-97 4.4946426759072655E-4 98-99 6.37949283031999E-4 100 6.669469777152716E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3448550.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.7366872663172 #Duplication Level Percentage of deduplicated Percentage of total 1 66.27511069578483 32.30029343521019 2 16.447080096336304 16.03152398198425 3 6.918258821316527 10.115190498058315 4 3.5122777488466794 6.847071289519407 5 2.0460111410719217 4.985790256291152 6 1.2516961931918091 3.6602115552017374 7 0.8334558175120472 2.843391286986417 8 0.577047523216361 2.2498707741438957 9 0.43148152790539557 1.8926082258046182 >10 1.6260867763419107 12.821291462136333 >50 0.05548964310525672 1.8301718571856997 >100 0.023743865259774607 2.0488048394286618 >500 0.0012328091515561594 0.42443656656915824 >1k 7.191386717410927E-4 0.5374928090267681 >5k 1.0273409596301325E-4 0.30106606992808166 >10k+ 2.054681919260265E-4 1.1107850925252771 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11586 0.33596729060039726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10599 0.30734656594800713 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6013 0.17436313813051862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.8997694683272678E-5 2 0.0 0.0 0.0 0.0 2.8997694683272678E-5 3 0.0 0.0 0.0 0.0 2.8997694683272678E-5 4 0.0 0.0 0.0 0.0 2.8997694683272678E-5 5 0.0 2.8997694683272678E-5 0.0 0.0 2.8997694683272678E-5 6 0.0 2.8997694683272678E-5 0.0 0.0 5.7995389366545356E-5 7 0.0 2.8997694683272678E-5 0.0 0.0 5.7995389366545356E-5 8 0.0 2.8997694683272678E-5 0.0 0.0 5.7995389366545356E-5 9 0.0 2.8997694683272678E-5 0.0 0.0 5.7995389366545356E-5 10-11 0.0 2.8997694683272678E-5 0.0 0.0 8.699308404981803E-5 12-13 0.0 5.7995389366545356E-5 0.0 0.0 2.6097925214945414E-4 14-15 0.0 8.699308404981803E-5 0.0 2.8997694683272678E-5 3.3347348885763583E-4 16-17 0.0 8.699308404981803E-5 0.0 5.7995389366545356E-5 4.349654202490902E-4 18-19 0.0 1.1599077873309071E-4 0.0 5.7995389366545356E-5 4.929608096156356E-4 20-21 0.0 1.7398616809963607E-4 0.0 5.7995389366545356E-5 5.219585042989083E-4 22-23 0.0 1.7398616809963607E-4 0.0 1.4498847341636338E-4 5.509561989821809E-4 24-25 0.0 1.7398616809963607E-4 0.0 5.799538936654535E-4 5.509561989821809E-4 26-27 0.0 1.7398616809963607E-4 0.0 0.001449884734163634 5.799538936654536E-4 28-29 0.0 1.7398616809963607E-4 0.0 0.00455263806527381 5.799538936654536E-4 30-31 0.0 1.7398616809963607E-4 0.0 0.013933392295312522 5.9445274100709E-4 32-33 0.0 1.7398616809963607E-4 0.0 0.03418828203157849 6.37949283031999E-4 34-35 0.0 1.7398616809963607E-4 0.0 0.06589726116773717 6.37949283031999E-4 36-37 0.0 1.7398616809963607E-4 0.0 0.11514984558727581 6.37949283031999E-4 38-39 0.0 1.7398616809963607E-4 0.0 0.1829319569094257 6.669469777152717E-4 40-41 0.0 1.7398616809963607E-4 0.0 0.2525554218439634 6.669469777152717E-4 42-43 0.0 1.7398616809963607E-4 0.0 0.32178741790027693 7.394412144234534E-4 44-45 0.0 1.884850154412724E-4 0.0 0.4058082382450595 7.829377564483623E-4 46-47 0.0 2.0298386278290876E-4 0.0 0.5081121050876456 8.264342984732714E-4 48-49 0.0 2.0298386278290876E-4 0.0 0.6147221295906975 8.699308404981804E-4 50-51 0.0 2.0298386278290876E-4 0.0 0.7198242739702194 9.279262298647257E-4 52-53 0.0 2.174827101245451E-4 0.0 0.8228530831798873 9.859216192312711E-4 54-55 0.0 3.044757941743631E-4 0.0 0.9346826927259283 9.859216192312711E-4 56-57 0.0 3.4797233619927214E-4 0.0 1.0619535746908122 0.0010294181612561801 58-59 0.0 4.059677255658175E-4 0.0 1.2056661495411114 0.0010439170085978166 60-61 0.0 4.059677255658175E-4 0.0 1.3540618520827594 0.001072914703281089 62-63 0.0 4.059677255658175E-4 0.0 1.4999927505763293 0.0011309100926476345 64-65 0.0 4.349654202490902E-4 0.0 1.651230227196938 0.0011309100926476345 66-67 0.0 4.929608096156356E-4 0.0 1.819822824085485 0.0011309100926476345 68-69 0.0 4.929608096156356E-4 0.0 2.0061475112728537 0.0011309100926476345 70-71 0.0 5.219585042989083E-4 0.0 2.2033608328137912 0.0011309100926476345 72-73 0.0 5.509561989821809E-4 0.0 2.3998927085296717 0.0011309100926476345 74-75 0.0 5.509561989821809E-4 0.0 2.6020791347087906 0.001145408939989271 76-77 0.0 5.509561989821809E-4 0.0 2.8188514013135957 0.0011599077873309072 78-79 0.0 6.089515883487262E-4 0.0 3.053312261675197 0.0011599077873309072 80-81 0.0 6.669469777152717E-4 0.0 3.296080961563556 0.0011599077873309072 82-83 0.0 6.669469777152717E-4 0.0 3.5492598338432098 0.0011599077873309072 84-85 0.0 6.669469777152717E-4 0.0 3.8039030897043684 0.0011599077873309072 86-87 0.0 6.959446723985443E-4 0.0 4.069942439576053 0.001232402024039089 88 0.0 6.959446723985443E-4 0.0 4.281045656870279 0.0013048962607472706 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6660 0.0 60.055298 1 GTATCAA 12115 0.0 42.957436 1 CAACGCA 15765 0.0 32.593864 5 ATCAACG 15840 0.0 32.528572 3 TATCAAC 16430 0.0 31.644613 2 AACGCAG 17060 0.0 30.633516 6 TCAACGC 17245 0.0 29.932898 4 GTGGTAT 1975 0.0 28.590435 1 ACGCAGA 18530 0.0 27.746403 7 CGCAGAG 18900 0.0 27.17835 8 GCAGAGT 20525 0.0 24.889212 9 GTACATG 12040 0.0 23.91837 1 TACATGG 11770 0.0 23.785683 2 ACATGGG 12080 0.0 22.844454 3 GTACTAG 1530 0.0 22.758673 1 GAGTACT 14105 0.0 22.49031 12-13 AGAGTAC 19340 0.0 22.210302 10-11 CAGAGTA 20200 0.0 22.102276 10-11 TGGTATC 2545 0.0 21.81571 2 CATGGGG 9745 0.0 21.274843 4 >>END_MODULE