FastQCFastQC Report
Mon 16 Jan 2017
ERR1146561_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146561_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3732861
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA116150.31115543814784424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14000.023.1743531
GTATTAC9050.020.7825091
GTATAAG17450.020.748271
CATGGGG80350.020.3544244
TGATCGC8800.019.2250659
GTATTAT14850.018.0482461
GGGCTAT22450.018.0023866
CTAACGC6300.017.9034253
TCTAACG6200.017.4341832
GGCTATT23050.017.3287357
TAACGCC6600.017.0896344
GTGATCG10450.017.0889458
TAGTACT18750.017.0440624
ATGGGGG50750.016.5761015
GTATAGA13350.016.5540071
ATTTAGG24750.016.5283931
AGGGCTA25300.016.3466075
GCTATTG23900.016.3203018
TACCTGG42000.016.224982
GTACATG253200.016.0077461
Produced by FastQC (version 0.11.5)