FastQCFastQC Report
Mon 16 Jan 2017
ERR1146561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3732861
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120660.32323732386499254No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA113220.3033062307972357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107790.28875974755020345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59430.15920764260978376No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50120.13426698717150198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12800.022.4286731
GAGTACT127000.022.16642212-13
AGTACTT135350.020.2607312-13
GTACTTT140350.019.99099314-15
CATGGGG78850.019.5593364
CTCGTCG9750.018.316889
TCTAACG5450.018.1335662
CTAACGC5450.018.117773
TGATCGC7350.017.9037179
ATGGGGG48100.017.5958165
GTATCAA300850.017.426851
ACTTTTT162700.017.33150116-17
TACTTTT158700.017.0724114-15
CGTTAAC3602.1609594E-916.9951321
TAACGCC5900.016.7359074
TAGGACT20400.016.3647774
GACTCGT11500.016.346877
GTAGGAC15200.016.0857643
TACCTGG43250.015.777652
GGGCTAT21850.015.7020216
Produced by FastQC (version 0.11.5)