FastQCFastQC Report
Mon 16 Jan 2017
ERR1146560_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146560_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4028412
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT558021.3852108473512639No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG448941.1144341740616401No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA191320.47492659638587115No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA168280.41773284361182517No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA151210.3753588262570958No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC132690.32938537567656934No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT128850.3198530835475617No Hit
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA109750.2724398596767163No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA94050.23346668612842977No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG77740.1929792682575665No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC46580.11562868941905644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12600.024.999571
TACCTGG39000.024.3385072
GTACCCG3700.021.601081
CATGGGG70300.021.322984
CGTTAAC2701.1405064E-920.8951631
GTACCTG48800.020.2314831
ACCTGGG46700.019.4201683
GTGATCG6800.019.348188
TGATCGC7150.018.4010669
ATGGGGG42000.017.565385
TACCCCG2957.183189E-817.5217635
GTATTAT13300.016.6140121
CGACGAG6800.016.58343524-25
TACGCAG12900.016.392134
GCATCGT13550.016.2985738
TAGTACT21350.016.2871994
GTCCTAC15100.016.1902921
TAGGCAT20300.015.9720345
ATGGGGA31850.015.9338775
GGGCTAT20350.015.9321976
Produced by FastQC (version 0.11.5)