##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146560_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4028412
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.649374244739615	33.0	31.0	34.0	30.0	34.0
2	31.80900141296372	33.0	31.0	34.0	30.0	34.0
3	31.87567408696032	34.0	31.0	34.0	30.0	34.0
4	35.34485127141911	37.0	35.0	37.0	33.0	37.0
5	35.41324646039184	37.0	35.0	37.0	33.0	37.0
6	35.42474776661374	37.0	35.0	37.0	33.0	37.0
7	35.42557042328342	37.0	35.0	37.0	33.0	37.0
8	35.388513885868676	37.0	35.0	37.0	33.0	37.0
9	37.02498379013865	39.0	38.0	39.0	34.0	39.0
10-11	37.01358053744255	39.0	38.0	39.0	34.0	39.0
12-13	36.99610590972324	39.0	38.0	39.0	34.0	39.0
14-15	38.36924152743066	40.0	39.0	41.0	34.0	41.0
16-17	38.36231919674552	40.0	38.5	41.0	34.0	41.0
18-19	38.43736675891145	40.5	39.0	41.0	34.0	41.0
20-21	38.52641636456251	41.0	39.0	41.0	34.0	41.0
22-23	38.52837991744638	41.0	39.0	41.0	34.0	41.0
24-25	38.55658594503244	41.0	39.0	41.0	34.0	41.0
26-27	38.523800445436066	40.5	39.0	41.0	34.0	41.0
28-29	38.447821374774975	40.0	38.0	41.0	34.0	41.0
30-31	38.437950984159514	40.0	38.0	41.0	34.0	41.0
32-33	38.419706450085044	40.0	38.0	41.0	33.0	41.0
34-35	38.31820714961627	40.0	38.0	41.0	33.0	41.0
36-37	38.20547364569463	40.0	38.0	41.0	33.0	41.0
38-39	38.13355684572482	40.0	38.0	41.0	33.0	41.0
40-41	38.07430657539497	40.0	38.0	41.0	33.0	41.0
42-43	37.93557151056049	40.0	38.0	41.0	32.5	41.0
44-45	37.80639976745179	40.0	38.0	41.0	32.0	41.0
46-47	37.68316137971985	40.0	37.0	41.0	32.0	41.0
48-49	37.55052623713761	40.0	37.0	41.0	31.0	41.0
50-51	37.367064615039375	40.0	37.0	41.0	31.0	41.0
52-53	37.26409835935351	40.0	36.0	41.0	31.0	41.0
54-55	37.08429574730688	39.5	36.0	41.0	30.0	41.0
56-57	36.83110094002302	39.0	35.0	41.0	30.0	41.0
58-59	36.63523095452997	39.0	35.0	41.0	30.0	41.0
60-61	36.389064102678674	39.0	35.0	41.0	29.0	41.0
62-63	36.156693009553145	38.0	35.0	40.0	29.0	41.0
64-65	35.8933625706606	38.0	35.0	40.0	29.0	41.0
66-67	35.51866380598608	37.0	34.5	40.0	28.5	41.0
68-69	35.195114476870785	36.5	34.0	39.5	28.0	41.0
70-71	34.793861824460855	36.0	34.0	39.0	28.0	41.0
72-73	34.43884674655919	35.5	34.0	39.0	27.5	40.5
74-75	34.15805793945604	35.0	33.0	37.5	27.0	40.0
76-77	33.80306942288922	35.0	33.0	37.0	26.5	39.0
78-79	33.4533404229756	35.0	33.0	37.0	26.0	39.0
80-81	33.09169940909719	35.0	33.0	36.0	26.0	39.0
82-83	32.83564578300333	35.0	32.5	36.0	26.0	37.0
84-85	32.63116967678579	35.0	32.0	35.5	26.0	37.0
86-87	32.51247613203416	35.0	32.0	35.0	26.0	36.5
88-89	32.30256897755244	35.0	32.0	35.0	25.0	36.0
90-91	32.06744369741725	35.0	32.0	35.0	25.0	36.0
92-93	31.904181970463796	35.0	32.0	35.0	25.0	36.0
94-95	31.786338760782165	35.0	32.0	35.0	24.5	35.5
96-97	31.71091027928623	35.0	32.0	35.0	24.0	35.0
98-99	31.68691025148371	35.0	32.0	35.0	24.0	35.0
100	30.596428816119104	34.0	29.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20716422549950053
1101	2	0.2276244867313011
1101	3	0.33588448604255206
1101	4	0.2558521692772189
1101	5	0.23325067546023348
1101	6	0.2663564660405484
1101	7	0.2967709618129106
1101	8	0.27615578824990195
1101	9	0.42598320595312344
1101	10-11	0.39049008571900146
1101	12-13	0.412330915477952
1101	14-15	0.5026118327939031
1101	16-17	0.4752926969102518
1101	18-19	0.3792908283516212
1101	20-21	0.40802066573144913
1101	22-23	0.42222983617481447
1101	24-25	0.39090551713346144
1101	26-27	0.40313554898431647
1101	28-29	0.43862652410924596
1101	30-31	0.4697555387561181
1101	32-33	0.47749036185485494
1101	34-35	0.47441937312109417
1101	36-37	0.48526782778495203
1101	38-39	0.47791051572530563
1101	40-41	0.4926324379367557
1101	42-43	0.5195528852832965
1101	44-45	0.42596206862583585
1101	46-47	0.4442043555309141
1101	48-49	0.5132372832947212
1101	50-51	0.5034859638295757
1101	52-53	0.5226000999760387
1101	54-55	0.5359201862581102
1101	56-57	0.46308623561616713
1101	58-59	0.518658381654987
1101	60-61	0.5215935282921507
1101	62-63	0.40908647639151496
1101	64-65	0.4601269879005443
1101	66-67	0.4328260437807856
1101	68-69	0.30245567722582933
1101	70-71	0.30664652824832217
1101	72-73	0.361660851866624
1101	74-75	0.46641565146273223
1101	76-77	0.465909670228406
1101	78-79	0.42207006918166456
1101	80-81	0.36028005852683975
1101	82-83	0.41452034324331066
1101	84-85	0.4768944882199193
1101	86-87	0.41325961471221717
1101	88-89	0.36226105759616445
1101	90-91	0.46770090809155107
1101	92-93	0.4023375983287174
1101	94-95	0.41345615631006893
1101	96-97	0.38584883125355773
1101	98-99	0.3448611124850238
1101	100	0.3170113351543904
1102	1	0.2886373578800203
1102	2	0.30280202042693105
1102	3	0.3567765664177429
1102	4	0.2823412382639887
1102	5	0.27663577520289806
1102	6	0.2495036529442558
1102	7	0.24022039024993092
1102	8	0.2737015833186973
1102	9	0.2963923761220144
1102	10-11	0.4012669521882799
1102	12-13	0.3669747736790754
1102	14-15	0.47924838864360453
1102	16-17	0.45863151604539354
1102	18-19	0.4218380485472579
1102	20-21	0.39176758251347366
1102	22-23	0.39354873430701076
1102	24-25	0.36649962607620523
1102	26-27	0.41748526387220153
1102	28-29	0.45390349537945696
1102	30-31	0.45212252622654603
1102	32-33	0.44596502133477145
1102	34-35	0.4423580544273591
1102	36-37	0.4663167520612035
1102	38-39	0.4510091253762383
1102	40-41	0.47035414924445007
1102	42-43	0.3947712834256478
1102	44-45	0.3070358751113389
1102	46-47	0.4116361721305495
1102	48-49	0.3865188439334375
1102	50-51	0.4160493223893056
1102	52-53	0.3697427828906328
1102	54-55	0.4411320927006983
1102	56-57	0.40573726870302096
1102	58-59	0.4518733705047495
1102	60-61	0.39949325034904604
1102	62-63	0.3813653647005637
1102	64-65	0.35564584456491843
1102	66-67	0.3735618670496663
1102	68-69	0.3629366234044582
1102	70-71	0.4013931577422696
1102	72-73	0.38396512395112836
1102	74-75	0.30553154033345464
1102	76-77	0.3010086925966817
1102	78-79	0.33586185011806435
1102	80-81	0.31544237779613127
1102	82-83	0.3345687477762951
1102	84-85	0.35894880007145247
1102	86-87	0.3353884819290869
1102	88-89	0.2434888788476215
1102	90-91	0.36734703239142164
1102	92-93	0.3656767478187497
1102	94-95	0.30040007871920693
1102	96-97	0.32973714897712014
1102	98-99	0.3027825738543619
1102	100	0.3139644901529053
1103	1	0.14423556836019458
1103	2	0.14800423630042658
1103	3	0.1702926906522677
1103	4	0.13398797827588993
1103	5	0.07710280582724494
1103	6	0.10533168822730232
1103	7	0.09802691570641997
1103	8	0.08710758393890927
1103	9	0.17141812278637758
1103	10-11	0.22662056991171653
1103	12-13	0.1886312443585041
1103	14-15	0.1984619048713796
1103	16-17	0.18513184674905148
1103	18-19	0.12778773381485564
1103	20-21	0.17477155206669437
1103	22-23	0.1415531783014714
1103	24-25	0.18449677928093422
1103	26-27	0.1790449152603415
1103	28-29	0.22522705845303648
1103	30-31	0.20086161454057105
1103	32-33	0.19023056893645673
1103	34-35	0.23354267759123104
1103	36-37	0.18291272464315966
1103	38-39	0.12253395802939337
1103	40-41	0.21438582283717267
1103	42-43	0.13951606104065917
1103	44-45	0.12176552237778537
1103	46-47	0.14501142614890483
1103	48-49	0.09531983591867288
1103	50-51	0.09795803900988886
1103	52-53	0.12019812443691791
1103	54-55	0.15910843779334982
1103	56-57	0.10992741675899964
1103	58-59	0.1741902426627817
1103	60-61	0.15072040016131183
1103	62-63	0.16756107011627108
1103	64-65	0.17261755019184477
1103	66-67	0.20994596593934745
1103	68-69	0.20843128810393807
1103	70-71	0.19210616857736085
1103	72-73	0.18516637363597965
1103	74-75	0.13364170595597358
1103	76-77	0.2168654132692751
1103	78-79	0.16235421923200732
1103	80-81	0.17443312038651015
1103	82-83	0.12755371453471298
1103	84-85	0.23877851375967651
1103	86-87	0.19649904862638579
1103	88-89	0.16131486342040802
1103	90-91	0.1721735418448489
1103	92-93	0.21502843848987752
1103	94-95	0.1961153826252584
1103	96-97	0.13537078169038708
1103	98-99	0.065077978933612
1103	100	0.10277198897197692
1104	1	0.1057429604138953
1104	2	0.13215830477159685
1104	3	0.1138995378324239
1104	4	0.11985685811377067
1104	5	0.1226183976534756
1104	6	0.14778400577897344
1104	7	0.11943731970067262
1104	8	0.1350946671797857
1104	9	0.20684749157251048
1104	10-11	0.18821810061144362
1104	12-13	0.23840401736772776
1104	14-15	0.26221168097323755
1104	16-17	0.2307516932071607
1104	18-19	0.24636763333221268
1104	20-21	0.24915363268646473
1104	22-23	0.2676294739409215
1104	24-25	0.2256217903463522
1104	26-27	0.26535546041795754
1104	28-29	0.260091577118466
1104	30-31	0.29558500635494056
1104	32-33	0.3438436766616917
1104	34-35	0.28252874774676684
1104	36-37	0.3116084135192523
1104	38-39	0.3169800128588989
1104	40-41	0.3004007925943739
1104	42-43	0.30687608480045725
1104	44-45	0.25740610072446657
1104	46-47	0.20025214215805676
1104	48-49	0.20849617467508352
1104	50-51	0.19723513549627114
1104	52-53	0.1282856186882526
1104	54-55	0.1243862377614704
1104	56-57	0.14214768119776267
1104	58-59	0.14769855780363628
1104	60-61	0.263247930284102
1104	62-63	0.19087068871041168
1104	64-65	0.17720419216419714
1104	66-67	0.17921572808252506
1104	68-69	0.23024791294994884
1104	70-71	0.13369321833314984
1104	72-73	0.21153849398803004
1104	74-75	0.17416601510575447
1104	76-77	0.15654808660945463
1104	78-79	0.2011336239805459
1104	80-81	0.18183556795098355
1104	82-83	0.17432085354497673
1104	84-85	0.20120806243684086
1104	86-87	0.22670081487331117
1104	88-89	0.18516499786112206
1104	90-91	0.21219834875871157
1104	92-93	0.23317258946266506
1104	94-95	0.20527191700464442
1104	96-97	0.13801345576937862
1104	98-99	0.14610224540184902
1104	100	0.12648305286047545
1105	1	0.14522569298812726
1105	2	0.16151011278974536
1105	3	0.1847579060828508
1105	4	0.14989720256805583
1105	5	0.137120338364781
1105	6	0.08524756891878127
1105	7	0.0812123075030442
1105	8	0.07018250316740904
1105	9	0.11131467504927883
1105	10-11	0.12351848667454846
1105	12-13	0.23139398675886014
1105	14-15	0.23549443445153173
1105	16-17	0.24579525587484596
1105	18-19	0.18645256463433668
1105	20-21	0.2123187542652687
1105	22-23	0.18923695043286415
1105	24-25	0.15268067966229637
1105	26-27	0.21917048100071668
1105	28-29	0.24974353365349344
1105	30-31	0.27431905678102453
1105	32-33	0.3096230317390791
1105	34-35	0.2134330221784424
1105	36-37	0.18444836096177397
1105	38-39	0.2203784866103078
1105	40-41	0.21732971747139374
1105	42-43	0.21826985146159217
1105	44-45	0.15464452897029446
1105	46-47	0.14517934160520696
1105	48-49	0.06345766454119683
1105	50-51	0.15159748907716164
1105	52-53	0.1346038683271189
1105	54-55	0.252220921609954
1105	56-57	0.16872119155861043
1105	58-59	0.2188109101512694
1105	60-61	0.18094468403196373
1105	62-63	0.18815941716335516
1105	64-65	0.21299717792562944
1105	66-67	0.17760811121483044
1105	68-69	0.2281073813953114
1105	70-71	0.21879497436426476
1105	72-73	0.2524067087365722
1105	74-75	0.34941171488168266
1105	76-77	0.2423993465165779
1105	78-79	0.20526464395241106
1105	80-81	0.20335328342039105
1105	82-83	0.2290374671839288
1105	84-85	0.24570166304074803
1105	86-87	0.22516169739910197
1105	88-89	0.2309856128148482
1105	90-91	0.24039793341202653
1105	92-93	0.23526842590796448
1105	94-95	0.2477646003324807
1105	96-97	0.16046554732998786
1105	98-99	0.18695299595026782
1105	100	0.20799458318890274
1106	1	0.1471754242792045
1106	2	0.14907062771748514
1106	3	0.13853369203336996
1106	4	0.17045316283186196
1106	5	0.14065229745722263
1106	6	0.11655068687632308
1106	7	0.12295323773875566
1106	8	0.1279635754123305
1106	9	0.18675696278532428
1106	10-11	0.2040201639654029
1106	12-13	0.19203166811036
1106	14-15	0.23487981437001793
1106	16-17	0.2150978907069927
1106	18-19	0.19583051464584855
1106	20-21	0.16757250633673237
1106	22-23	0.22546633812111594
1106	24-25	0.21840867564329614
1106	26-27	0.14874274146115596
1106	28-29	0.20487420077204632
1106	30-31	0.1684502033960129
1106	32-33	0.1852047032046471
1106	34-35	0.1854191961455598
1106	36-37	0.2318620385858594
1106	38-39	0.23477125304599866
1106	40-41	0.2090138702166513
1106	42-43	0.16109027191954084
1106	44-45	0.1662784947371847
1106	46-47	0.17749621822316186
1106	48-49	0.19579543136266153
1106	50-51	0.1340299954960784
1106	52-53	0.20733992802135504
1106	54-55	0.2971774600464272
1106	56-57	0.194559107246441
1106	58-59	0.1978625958912943
1106	60-61	0.13639272761864873
1106	62-63	0.15064633909126002
1106	64-65	0.1467664443655039
1106	66-67	0.1738197895643836
1106	68-69	0.19212458962157797
1106	70-71	0.17812329436431895
1106	72-73	0.21059083252901445
1106	74-75	0.15934601645385982
1106	76-77	0.22322622621283017
1106	78-79	0.17365978562317963
1106	80-81	0.18334280360680566
1106	82-83	0.18903357946025068
1106	84-85	0.23614710876337597
1106	86-87	0.1827646822368223
1106	88-89	0.19770859151803677
1106	90-91	0.2456455241357034
1106	92-93	0.2831458218715106
1106	94-95	0.24634513940387137
1106	96-97	0.2363491327263496
1106	98-99	0.30965148010092847
1106	100	0.12980210962629357
1107	1	0.09001948442624652
1107	2	0.08509378353803498
1107	3	0.17965630958973122
1107	4	0.11777793592320052
1107	5	0.07150550628004027
1107	6	0.11127164045027627
1107	7	0.11800708000680515
1107	8	0.08567743233646752
1107	9	0.1258536045343277
1107	10-11	0.19473266449490012
1107	12-13	0.164189672668563
1107	14-15	0.133227488255514
1107	16-17	0.11317610110751986
1107	18-19	0.07017763986438297
1107	20-21	0.08583364990968079
1107	22-23	0.14046936818051137
1107	24-25	0.19597718769250605
1107	26-27	0.2192541976215452
1107	28-29	0.16360273136757542
1107	30-31	0.18923992649497734
1107	32-33	0.19505752559764744
1107	34-35	0.20257146477106147
1107	36-37	0.20671410166296766
1107	38-39	0.23477711721231032
1107	40-41	0.2353273354087193
1107	42-43	0.20649481150468318
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2216	64-65	-0.9569071921496004
2216	66-67	-1.0850000261691477
2216	68-69	-1.072936866084362
2216	70-71	-1.1177935214604489
2216	72-73	-1.0445362977120283
2216	74-75	-0.945519492840198
2216	76-77	-0.9832410997047845
2216	78-79	-1.0188761847139602
2216	80-81	-1.0047297739607544
2216	82-83	-0.9690757062041655
2216	84-85	-0.9323608733899036
2216	86-87	-0.8807587853751997
2216	88-89	-0.9346016650338882
2216	90-91	-0.9461632442617116
2216	92-93	-0.9815951420174507
2216	94-95	-0.9807338332234252
2216	96-97	-0.9106372847852633
2216	98-99	-0.8778641906008211
2216	100	-0.9394353016658634
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	116.0
1	60.0
2	37.0
3	45.0
4	42.0
5	74.0
6	149.0
7	625.0
8	2000.0
9	2891.0
10	2883.0
11	3815.0
12	4732.0
13	5971.0
14	6928.0
15	7699.0
16	8383.0
17	9278.0
18	10297.0
19	11556.0
20	13254.0
21	15015.0
22	17923.0
23	20979.0
24	25673.0
25	32746.0
26	38254.0
27	38623.0
28	41507.0
29	53256.0
30	64291.0
31	74161.0
32	95461.0
33	130019.0
34	191469.0
35	292234.0
36	488009.0
37	894299.0
38	1082021.0
39	340782.0
40	855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79036605685342	18.65364342964561	12.956751380798284	22.59923913270269
2	13.336774210205798	22.420515371856755	39.22495365615511	25.017756761782334
3	20.863805883997337	27.140187222159607	30.863155427759388	21.13285146608367
4	11.483923534848664	17.133507661683836	40.87473507527623	30.50783372819127
5	11.73030210946329	41.15230996260127	34.984324508980265	12.13306341895518
6	27.890919003571586	37.06379940346175	20.244315532686212	14.800966060280455
7	25.234746882971	34.23810439952585	22.973853572558976	17.553295144944176
8	23.709609871055942	33.07547500968799	22.422502861713514	20.792412257542548
9	25.613554693560904	18.877020946470132	21.78199534522886	33.72742901474011
10-11	23.686210275370474	25.689692282064886	28.588886127755835	22.035211314808805
12-13	27.429189007764194	23.39013455363918	27.75427455218194	21.426401886414688
14-15	24.87165759536166	24.88628025683749	27.165404520785717	23.07665762701513
16-17	21.463742883295353	28.229357180929703	28.918479363201254	21.38842057257369
18-19	20.80736683509152	30.33391367210454	28.294854133715912	20.56386535908803
20-21	22.190333940687797	28.931174065952014	26.027428887208202	22.85106310615198
22-23	21.759777812200866	27.993790935341522	25.12257019331212	25.123861059145487
24-25	23.003340901541957	28.525708656901973	25.183007327740505	23.287943113815565
26-27	23.586749962452352	26.05894918717099	28.16123270480846	22.193068145568198
28-29	22.149480159675882	28.397419665554498	27.482051358436603	21.971048816333017
30-31	22.081622261659504	27.758182577176505	27.35561861051815	22.804576550645837
32-33	21.866326314775517	28.83129244675009	27.37110934789279	21.9312718905816
34-35	23.003603219572955	28.22810402029531	26.899653432021026	21.86863932811071
36-37	23.446853460331475	26.27969442882679	27.791970569588546	22.48148154125319
38-39	23.655208235312518	27.19774974420447	27.404136575570103	21.742905444912903
40-41	22.973259841346692	27.339937680781595	28.328392486934067	21.358409990937645
42-43	22.78253882669916	27.349859978440268	26.489022550566236	23.378578644294336
44-45	22.249417378201287	27.51773875575002	27.312686672694735	22.92015719335396
46-47	23.00915456532638	28.599207731680625	26.29333345914539	22.098304243847604
48-49	22.707254530923564	28.079949711103303	26.52412765761436	22.68866810035878
50-51	21.490085260181992	27.745242526542153	27.64027826579275	23.124393947483103
52-53	22.33046365180425	28.647173194012655	25.974818482503796	23.047544671679297
54-55	22.18335863812068	27.3107939445321	27.211387601299858	23.294459816047357
56-57	22.738626750503162	26.78278680636877	27.49754675323982	22.981039689888252
58-59	23.16761002783025	27.261937369891033	26.976942387382095	22.59351021489662
60-61	22.85254554141614	27.382797716547085	28.466973471299394	21.297683270737373
62-63	22.46902485548078	27.676161725447564	27.500909782932187	22.35390363613947
64-65	21.188685706424447	28.70069781873599	27.14613219792415	22.964484276915414
66-67	22.923269704939642	26.41767019604055	26.987343274716274	23.671716824303534
68-69	23.0439274836615	27.037819349370455	26.68700393604141	23.23124923092664
70-71	23.113024574425076	27.36342216178254	26.74029524275924	22.78325802103314
72-73	22.635123222167426	26.23297907053321	28.377751521373227	22.754146185926135
74-75	21.757266898088908	29.72166626498679	26.497002549270356	22.02406428765395
76-77	22.48284235177956	26.817671437105222	26.37936341533584	24.32012279577938
78-79	22.404625819121325	27.469718461656136	27.033958830617085	23.09169688860546
80-81	23.26866428348008	27.187697859887848	27.242842524296996	22.300795332335074
82-83	23.00069223056555	26.111210099737075	28.15119805554607	22.736899614151305
84-85	22.10852705093325	27.524102333352207	27.611201997717405	22.756168617997137
86-87	22.09435537690314	28.30196948768488	27.637763257957882	21.9659118774541
88-89	22.387148535739403	27.788980897666637	26.477446095901026	23.346424470692938
90-91	22.81476141687273	26.213526175135943	26.773176799188292	24.198535608803034
92-93	23.652428628844664	27.61177101185512	26.68770717218511	22.048093187115107
94-95	23.02882860935612	26.92503732135077	27.64601020077602	22.400123868517095
96-97	22.064912145214244	26.59365693388424	28.473286726762044	22.86814419413947
98-99	22.29486792849196	27.975291741279918	27.112027335423228	22.617812994804893
100	22.16626705389707	26.77911280005721	25.953607837839986	25.101012308205732
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1838.0
1	1242.5
2	677.0
3	684.5
4	591.0
5	488.5
6	424.0
7	404.0
8	439.5
9	471.0
10	429.0
11	367.0
12	394.0
13	410.5
14	431.0
15	517.5
16	593.0
17	734.0
18	937.0
19	1213.5
20	1786.0
21	2615.0
22	3872.5
23	5833.0
24	8108.5
25	10982.5
26	15451.5
27	20595.0
28	26026.0
29	32743.5
30	41015.0
31	48146.5
32	56971.0
33	67798.0
34	76428.0
35	84026.5
36	93363.0
37	102913.5
38	106725.0
39	107964.0
40	107866.0
41	106627.5
42	106550.0
43	120368.0
44	164767.5
45	197025.0
46	182905.0
47	200504.0
48	319615.5
49	323752.5
50	223013.0
51	173283.5
52	127079.0
53	107795.0
54	98831.0
55	87491.5
56	77967.5
57	67789.0
58	58713.0
59	53659.0
60	45438.0
61	35694.0
62	28660.5
63	22568.5
64	16208.0
65	10987.5
66	7964.0
67	5983.0
68	4493.0
69	3516.0
70	2970.0
71	2469.0
72	2051.5
73	1981.5
74	1642.5
75	1289.5
76	980.0
77	639.5
78	437.0
79	264.0
80	187.0
81	142.5
82	107.0
83	72.5
84	55.0
85	53.0
86	41.5
87	23.5
88	21.0
89	27.0
90	19.5
91	11.0
92	8.5
93	9.5
94	10.5
95	10.0
96	9.0
97	8.0
98	6.0
99	6.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06156271999984113
2	0.009159936967718297
3	0.011667128387066666
4	0.009457821096749787
5	0.011617481032228084
6	0.012560780774161133
7	0.0034008438064428363
8	0.00573426948385617
9	0.007124395419336453
10-11	0.006826511290304964
12-13	0.005994918096758723
14-15	0.0103638853225539
16-17	0.009656410516104112
18-19	0.007571221612883687
20-21	0.007111983580626808
22-23	0.002680957161283404
24-25	0.003922141032247943
26-27	0.004890264451600283
28-29	0.006255566709661276
30-31	0.00884964099997716
32-33	0.01052523922577929
34-35	0.012076719064484962
36-37	0.018406756806404113
38-39	0.019387292064466096
40-41	0.018915642193499574
42-43	0.024736794548323258
44-45	0.028361051451539714
46-47	0.030806183677339855
48-49	0.03178671893540184
50-51	0.032941019935398864
52-53	0.031079244128952056
54-55	0.03738445819345191
56-57	0.03985441409667134
58-59	0.037210692451516876
60-61	0.03793057909667631
62-63	0.033574023709590776
64-65	0.035621977096682265
66-67	0.038253286903127084
68-69	0.04097147958053943
70-71	0.04031365212892822
72-73	0.036192921677325955
74-75	0.039060056419254044
76-77	0.038811819645061134
78-79	0.03812916851603063
80-81	0.042957373774082694
82-83	0.039084880096673325
84-85	0.03515032722571575
86-87	0.03349955267733291
88-89	0.03127783354830638
90-91	0.03341266980636539
92-93	0.035187562741844675
94-95	0.03876217229022255
96-97	0.03398361438700907
98-99	0.031190950677338864
100	0.029291939354763118
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4028412.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.825006053746115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7630437034407	39.702962738804345
2	14.067985322074557	15.144187902493492
3	5.390160854482259	8.703763218695187
4	2.5419349248530425	5.472786508737747
5	1.3986309964468144	3.764066092535337
6	0.8030931429804902	2.5935895969588127
7	0.5201108778451934	1.9596479804045719
8	0.3396838373028771	1.4626787679349655
9	0.2339324314083607	1.1332273083050306
>10	0.8836159708740412	7.5838358755756525
>50	0.034367746694198266	1.2764409995767594
>100	0.02115068093542547	2.0303938320824977
>500	8.585664983206442E-4	0.30284491380716133
>1k	7.154720819269305E-4	0.9331813264647937
>5k	1.430944163853861E-4	0.6615682571532969
>10k+	5.723776655415444E-4	7.274824680470382
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	55802	1.3852108473512639	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	44894	1.1144341740616401	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	19132	0.47492659638587115	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	16828	0.41773284361182517	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	15121	0.3753588262570958	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	13269	0.32938537567656934	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	12885	0.3198530835475617	No Hit
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA	10975	0.2724398596767163	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	9405	0.23346668612842977	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	7774	0.1929792682575665	No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC	4658	0.11562868941905644	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4823677419290777E-5
2	0.0	0.0	0.0	0.0	2.4823677419290777E-5
3	0.0	0.0	0.0	0.0	2.4823677419290777E-5
4	0.0	0.0	0.0	0.0	2.4823677419290777E-5
5	0.0	0.0	0.0	0.0	2.4823677419290777E-5
6	0.0	0.0	0.0	0.0	4.964735483858155E-5
7	0.0	0.0	0.0	0.0	4.964735483858155E-5
8	0.0	0.0	0.0	0.0	4.964735483858155E-5
9	0.0	0.0	0.0	0.0	4.964735483858155E-5
10-11	0.0	0.0	0.0	6.205919354822695E-5	4.964735483858155E-5
12-13	0.0	0.0	0.0	1.241183870964539E-4	6.205919354822695E-5
14-15	0.0	0.0	0.0	1.8617758064468082E-4	7.447103225787233E-5
16-17	0.0	0.0	0.0	2.606486129025532E-4	9.92947096771631E-5
18-19	0.0	0.0	0.0	3.475314838700709E-4	9.92947096771631E-5
20-21	0.0	0.0	0.0	5.461209032243972E-4	1.4894206451574466E-4
22-23	0.0	0.0	0.0	9.184760645137588E-4	1.4894206451574466E-4
24-25	0.0	0.0	0.0	0.0016507745483828368	1.4894206451574466E-4
26-27	0.0	0.0	0.0	0.0026064861290255315	1.7376574193503545E-4
28-29	0.0	0.0	0.0	0.006677569225789219	1.7376574193503545E-4
30-31	0.0	0.0	0.0	0.015266561612863827	1.985894193543262E-4
32-33	0.0	0.0	0.0	0.03498897332249035	2.358249354832624E-4
34-35	0.0	0.0	0.0	0.07068542145143049	2.482367741929078E-4
36-37	0.0	0.0	0.0	0.12569469061257885	2.482367741929078E-4
38-39	0.0	0.0	0.0	0.20201508683818836	2.606486129025532E-4
40-41	0.0	0.0	0.0	0.27798795157992773	2.7306045161219853E-4
42-43	0.0	0.0	0.0	0.34395687432169303	2.7306045161219853E-4
44-45	0.0	0.0	0.0	0.420575154676334	2.854722903218439E-4
46-47	0.0	0.0	0.0	0.5146320684180268	2.978841290314893E-4
48-49	0.0	0.0	0.0	0.6129710665145471	3.599433225797163E-4
50-51	0.0	0.0	0.0	0.716510625030409	3.723551612893617E-4
52-53	0.0	0.0	0.0	0.8163886911269255	3.971788387086524E-4
54-55	0.0	0.0	0.0	0.9205240179008503	3.971788387086524E-4
56-57	0.0	0.0	0.0	1.0454739981908503	3.971788387086524E-4
58-59	0.0	0.0	0.0	1.1828854645453344	3.971788387086524E-4
60-61	0.0	0.0	0.0	1.327632327577219	3.971788387086524E-4
62-63	0.0	0.0	0.0	1.4813281263187579	4.220025161279432E-4
64-65	0.0	0.0	0.0	1.6509855496409007	4.220025161279432E-4
66-67	0.0	0.0	0.0	1.819327318059821	4.220025161279432E-4
68-69	0.0	0.0	0.0	1.9940984189303377	4.220025161279432E-4
70-71	0.0	0.0	0.0	2.182572189736303	4.344143548375886E-4
72-73	0.0	0.0	0.0	2.360409014768102	4.592380322568794E-4
74-75	0.0	0.0	0.0	2.53828307531603	4.716498709665248E-4
76-77	0.0	0.0	0.0	2.7251060715736126	4.716498709665248E-4
78-79	0.0	0.0	0.0	2.951895188476253	4.716498709665248E-4
80-81	0.0	0.0	0.0	3.18477851818533	4.716498709665248E-4
82-83	0.0	0.0	0.0	3.411505575894422	5.212972258051064E-4
84-85	0.0	1.2411838709645388E-5	0.0	3.6452701461518835	5.212972258051064E-4
86-87	0.0	2.4823677419290777E-5	0.0	3.882435560215787	5.337090645147517E-4
88	0.0	2.4823677419290777E-5	0.0	4.071505099279816	5.461209032243971E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	1260	0.0	24.99957	1
TACCTGG	3900	0.0	24.338507	2
GTACCCG	370	0.0	21.60108	1
CATGGGG	7030	0.0	21.32298	4
CGTTAAC	270	1.1405064E-9	20.895163	1
GTACCTG	4880	0.0	20.231483	1
ACCTGGG	4670	0.0	19.420168	3
GTGATCG	680	0.0	19.34818	8
TGATCGC	715	0.0	18.401066	9
ATGGGGG	4200	0.0	17.56538	5
TACCCCG	295	7.183189E-8	17.521763	5
GTATTAT	1330	0.0	16.614012	1
CGACGAG	680	0.0	16.583435	24-25
TACGCAG	1290	0.0	16.39213	4
GCATCGT	1355	0.0	16.298573	8
TAGTACT	2135	0.0	16.287199	4
GTCCTAC	1510	0.0	16.190292	1
TAGGCAT	2030	0.0	15.972034	5
ATGGGGA	3185	0.0	15.933877	5
GGGCTAT	2035	0.0	15.932197	6
>>END_MODULE